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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
21/04/2024 |
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Data da última atualização: |
23/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ONORATO, G. de C.; AMARAL, D. L. A. S. do; OLIVEIRA, L. F. C. de; BRANDAO, H. de M.; MUNK, M. |
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Afiliação: |
GEOVANA DE CARVALHO ONORATO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; DANIELLE LUCIANA AURORA SOARES DO AMARAL, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; LUIZ FERNANDO CAPPA DE OLIVEIRA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; HUMBERTO DE MELLO BRANDAO, CNPGL; MICHELE MUNK, UNIVERSIDADE FEDERAL DE JUIZ DE FORA. |
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Título: |
Barium titanate nanoparticles exhibit cytocompatibility in cultured bovine fibroblasts: a model for dermal exposure. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Current Journal of Applied Science and Technology, v. 43, n. 5, article CJAST.115461, 2024. |
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DOI: |
https://doi.org/10.9734/cjast/2024/v43i54372 |
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Idioma: |
Inglês |
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Conteúdo: |
The emergence of barium titanate nanoparticles (BaTiO3 NPs) represents an advancement in various fields such as technology, health, and agribusiness. However, increased production heightens the risk of their dispersion into the environment, thereby raising concerns about potential exposure to animals and humans, including the risk of dermal exposure. This study explores the chemical-physical properties of BaTiO3 NPs and their cytocompatibility using a bovine fibroblast cell model. The size and Zeta potential of the NPs were analyzed using scanning electron microscopy and dynamic light scattering technique. Raman spectroscopy was used to characterize the composition of the BaTiO3 NPs. Bovine fibroblasts were exposed in vitro to NPs (0.1 to 100 μg mL-1 ) for 24 hours to evaluate the cytocompatibility using the Thiazolyl Blue Tetrazolium Bromide assay and Trypan Blue exclusion test. The data were evaluated by analysis of variance and the means compared by the Tukey test. Scanning electron microscopy revealed that BaTiO3 NPs measured approximately 100 nm. Dynamic light scattering analysis indicated a hydrodynamic size of 149.27 nm with a polydispersion index of 0.37, and the Zeta potential was -13mV. Raman spectroscopy analysis highlighted the cubic phase of BaTiO3 NPs. Cytotoxicity tests demonstrated that BaTiO3 NPs did not affect cell viability, with 10 μg mL-1 resulting in enhanced cell proliferation. Overall, these findings underscore the non-toxic characteristics of BaTiO3 NPs in fibroblast cells, positioning them as promising and cytocompatible nanomaterials. MenosThe emergence of barium titanate nanoparticles (BaTiO3 NPs) represents an advancement in various fields such as technology, health, and agribusiness. However, increased production heightens the risk of their dispersion into the environment, thereby raising concerns about potential exposure to animals and humans, including the risk of dermal exposure. This study explores the chemical-physical properties of BaTiO3 NPs and their cytocompatibility using a bovine fibroblast cell model. The size and Zeta potential of the NPs were analyzed using scanning electron microscopy and dynamic light scattering technique. Raman spectroscopy was used to characterize the composition of the BaTiO3 NPs. Bovine fibroblasts were exposed in vitro to NPs (0.1 to 100 μg mL-1 ) for 24 hours to evaluate the cytocompatibility using the Thiazolyl Blue Tetrazolium Bromide assay and Trypan Blue exclusion test. The data were evaluated by analysis of variance and the means compared by the Tukey test. Scanning electron microscopy revealed that BaTiO3 NPs measured approximately 100 nm. Dynamic light scattering analysis indicated a hydrodynamic size of 149.27 nm with a polydispersion index of 0.37, and the Zeta potential was -13mV. Raman spectroscopy analysis highlighted the cubic phase of BaTiO3 NPs. Cytotoxicity tests demonstrated that BaTiO3 NPs did not affect cell viability, with 10 μg mL-1 resulting in enhanced cell proliferation. Overall, these findings underscore the non-toxic characteristics of BaTiO3 ... Mostrar Tudo |
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Palavras-Chave: |
Fibroblasto bovino; Modelos in vitro; Nanomaterial; Nanomaterial cerâmico; Piezoeletricidade; Titanato. |
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Thesagro: |
Bário. |
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Categoria do assunto: |
W Química e Física |
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Marc: |
LEADER 02467naa a2200265 a 4500
001 2163766
005 2024-04-23
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.9734/cjast/2024/v43i54372$2DOI
100 1 $aONORATO, G. de C.
245 $aBarium titanate nanoparticles exhibit cytocompatibility in cultured bovine fibroblasts$ba model for dermal exposure.$h[electronic resource]
260 $c2024
520 $aThe emergence of barium titanate nanoparticles (BaTiO3 NPs) represents an advancement in various fields such as technology, health, and agribusiness. However, increased production heightens the risk of their dispersion into the environment, thereby raising concerns about potential exposure to animals and humans, including the risk of dermal exposure. This study explores the chemical-physical properties of BaTiO3 NPs and their cytocompatibility using a bovine fibroblast cell model. The size and Zeta potential of the NPs were analyzed using scanning electron microscopy and dynamic light scattering technique. Raman spectroscopy was used to characterize the composition of the BaTiO3 NPs. Bovine fibroblasts were exposed in vitro to NPs (0.1 to 100 μg mL-1 ) for 24 hours to evaluate the cytocompatibility using the Thiazolyl Blue Tetrazolium Bromide assay and Trypan Blue exclusion test. The data were evaluated by analysis of variance and the means compared by the Tukey test. Scanning electron microscopy revealed that BaTiO3 NPs measured approximately 100 nm. Dynamic light scattering analysis indicated a hydrodynamic size of 149.27 nm with a polydispersion index of 0.37, and the Zeta potential was -13mV. Raman spectroscopy analysis highlighted the cubic phase of BaTiO3 NPs. Cytotoxicity tests demonstrated that BaTiO3 NPs did not affect cell viability, with 10 μg mL-1 resulting in enhanced cell proliferation. Overall, these findings underscore the non-toxic characteristics of BaTiO3 NPs in fibroblast cells, positioning them as promising and cytocompatible nanomaterials.
650 $aBário
653 $aFibroblasto bovino
653 $aModelos in vitro
653 $aNanomaterial
653 $aNanomaterial cerâmico
653 $aPiezoeletricidade
653 $aTitanato
700 1 $aAMARAL, D. L. A. S. do
700 1 $aOLIVEIRA, L. F. C. de
700 1 $aBRANDAO, H. de M.
700 1 $aMUNK, M.
773 $tCurrent Journal of Applied Science and Technology$gv. 43, n. 5, article CJAST.115461, 2024.
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Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
19/05/1998 |
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Data da última atualização: |
14/09/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
NOONAN, M. J.; FLEMING, C. H.; TUCKER, M. A.; KAYS, R.; HARRISON, AUTUMN-LYNN; CROFOOT, M. C.; ABRAHMS, B.; ALBERTS, S.; ALI, A. H.; ALTMANN, J.; ANTUNES, P. C.; ATTIAS, N.; BELANT, J. L.; BEYER JUNIOR, D. E.; BIDNER, L. R.; BLAUM, N.; BOONE, R. B.; CAILLAUD, D.; PAULA, R. C. de; DE LA TORRE, J. A.; DEKKER, J.; DEPERNO, C. S.; FARHADINIA, M.; FENNESSY, J.; FICHTEL, C.; FISCHER, C.; FORD, A.; GOHEEN, J. R.; HAVMØLLER, R. W.; HIRSCH, B. T.; HURTADO, C.; ISBELL, L. A.; JANSSEN, R.; JELTSCH, F.; KACZENSKY, P.; KANEKO, Y.; KAPPELER, P.; KATNA, A.; KAUFFMAN, M.; KOCH, F.; KULKARNI, A; LAPOINT, S.; LEIMGRUBER, P.; MACDONALD, D. W.; MARKHAM, A. C.; MCMAHON, L.; MERTES, K.; MOORMAN, C. E.; MORATO, R. G.; MOßBRUCKER, A. M.; MOURAO, G.; O'CONNOR, D.; OLIVEIRA-SANTOS, L. G. R.; PASTORINI, J.; PATTERSON, B. D.; RACHLOW, J.; RANGLACK, D. H.; REID, N.; SCANTLEBURY, D. M.; SCOTT, D. M.; SELVA, N.; SERGIEL, A.; SONGER, M.; SONGSASEN, N.; STABACH, J. A.; STACY-DAWES, J.; SWINGEN, M. B.; THOMPSON, J. J.; ULLMANN, W.; VANAK, A. T.; THAKER, M.; WILSON, J. W.; YAMAZAKI, K.; YARNELL, R. W.; ZIEBA, F.; ZWIJACZ-KOZICA, T.; FAGAN, W. F.; MUELLER, T.; CALABRESE, J. M. |
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Afiliação: |
MICHAEL J. NOONAN, Smithsonian Conservation Biology Institute, National Zoological Park; CHRISTEN H. FLEMING, University of Maryland; MARLEE A. TUCKER, Senckenberg Biodiversity and Climate Research Centre; ROLAND KAYS, Museum of Natural Sciences, Biodiversity Lab, Raleigh; AUTUMN-LYNN HARRISON, Smithsonian Conservation Biology Institute, Washington, D.C; MARGARET C. CROFOOT, University of California, Davis; BRIANA ABRAHMS, NOAA Southwest Fisheries Science Center; SUSAN C. ALBERTS, Duke University, Durham; ABDULLAHI H. ALI, Hirola Conservation Programme, Garissa; JEANNE ALTMANN, Princeton University; PAMELA CASTRO ANTUNES, Federal University of Mato Grosso do Sul, Campo Grande, MS; NINA ATTIAS, Universidade Federal do Mato Grosso do Sul, Campo Grande; JERROLD L. BELANT, College of Environmental Science and Forestry, Syracuse; DEAN E. BEYER JUNIOR, Michigan Department of Natural Resources; LAURA R. BIDNER, Mpala Research Centre, Nanyuki; NIELS BLAUM, University of Potsdam, Plant Ecology and Nature Conservation; RANDALL B. BOONE, Colorado State University, Fort Collins; DAMIEN CAILLAUD, Colorado State University; ROGERIO CUNHA DE PAULA, Chico Mendes Institute for the Conservation of Biodiversity; J. ANTONIO DE LA TORRE, Universidad Nacional Autónoma de Mexico and CONACyT; JASJA DEKKER, Jasja Dekker Dierecologie; CHRISTOPHER S. DEPERNO, University of Oxford, Tubney House; MOHAMMAD FARHADINIA, Future4Leopards Foundation, Tehran; JULIAN FENNESSY, Giraffe Conservation Foundation, PO; CLAUDIA FICHTEL, German Primate Center, Behavioral Ecology & Sociobiology Unit; CHRISTINA FISCHER, Restoration Ecology, Department of Ecology and Ecosystem Management; ADAM FORD, The University of British Columbia; JACOB R. GOHEEN, University of Wyoming, Laramie; RASMUS W. HAVMØLLER, University of California, Davis; BEN T. HIRSCH, James Cook University, Townsville; CINDY HURTADO, Universidad Nacional Mayor de San Marcos, Lima; LYNNE A. ISBELL, Mpala Research Centre, Nanyuki; RENÉ JANSSEN, 6Bionet Natuuronderzoek, Valderstraat; FLORIAN JELTSCH, University of Potsdam, Plant Ecology and Nature Conservation; PETRA KACZENSKY, Norwegian Institute for Nature Research - NINA; YAYOI KANEKO, Tokyo University of Agriculture and Technology, Tokyo; PETER KAPPELER, Ashoka Trust for Research in Ecology and the Environment (ATREE); ANJAN KATNA, Ashoka Trust for Research in Ecology and the Environment (ATREE), Bangalore; MATTHEW KAUFFMAN, University of Wyoming, Laramie, WY; FLAVIA KOCH, German Primate Center, Behavioral Ecology & Sociobiology Unit; ABHIJEET KULKARNI, Ashoka Trust for Research in Ecology and the Environment (ATREE); SCOTT LAPOINT, Manipal Academy of Higher Education, Manipal; PETER LEIMGRUBER, University of Wyoming; DAVID W. MACDONALD, Max Planck Institute for Ornithology; A. CATHERINE MARKHAM, Black Rock Forest; LAURA MCMAHON, Office of Applied Science, Department of Natural Resources; KATHERINE MERTES, Institute for the Conservation of Neotropical Carnivores; CHRISTOPHER E. MOORMAN, Frankfurt Zoological Society, Bernhard-Grzimek-Allee; RONALDO G. MORATO, National Research Center for Carnivores Conservation; ALEXANDER M. MOßBRUCKER, Frankfurt Zoological Society, Bernhard-Grzimek-Allee; GUILHERME DE MIRANDA MOURAO, CPAP; DAVID O'CONNOR, San Diego Zoo Institute of Conservation Research; LUIZ GUSTAVO R. OLIVEIRA-SANTOS, National Geographic Partners; JENNIFER PASTORINI, Federal University of Mato Grosso do Sul; BRUCE D. PATTERSON, Centre for Conservation and Research, Sri Lanka; JANET RACHLOW, Anthropologisches Institut, Switzerland; DUSTIN H. RANGLACK, University of Nebraska at Kearney, Kearney; NEIL REID, Queen's University Belfast, Belfast; DAVID M. SCANTLEBURY, Queen's University Belfast; DAWN M. SCOTT, Keele University, Keele; NURIA SELVA, Institute of Nature Conservation, Polish Academy of Sciences; AGNIESZKA SERGIEL, Treaty Authority, Duluth; MELISSA SONGER, Asociación Guyra Paraguay-CONACYT; NUCHARIN SONGSASEN, Instituto Saite, Paraguay; JARED A. STABACH, Wellcome Trust/DBT India Alliance, Hyderabad, India; JENNA STACY-DAWES, University of KwaZulu-Natal, Westville, Durban; MORGAN B. SWINGEN, Indian Institute of Science, Bangalore, India; JEFFREY J. THOMPSON, University of Pretoria; WIEBKE ULLMANN, Ibaraki Nature Museum, Osaki; ABI TAMIM VANAK, University of Agriculture, Tokyo; MARIA THAKER, Nottingham Trent University, Brackenhurst Campus; JOHN W. WILSON, University of Pretoria, Pretoria; KOJI YAMAZAKI, Ibaraki Nature Museum, Osaki; RICHARD W. YARNELL, Nottingham Trent University, Brackenhurst Campus; FILIP ZIEBA, Tatra National Park, Zakopane; TOMASZ ZWIJACZ-KOZICA, Tatra National Park, Zakopane; WILLIAM F. FAGAN, University of Maryland, College Park; THOMAS MUELLER, Senckenberg Gesellschaft für Naturforschung, Frankfurt; JUSTIN M. CALABRESE, National Zoological Park, Front Royal. |
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Título: |
Effects of body size on estimation of mammalian area requirements. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Conservation Biology, v.34, n. 4, p. 1017-1028, 2020. |
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DOI: |
10.1111/cobi.13495 |
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Idioma: |
Inglês |
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Conteúdo: |
Accurately quantifying species' area requirements is a prerequisite for effective area-based conservation. This typically involves collecting tracking data on species of interest and then conducting home range analyses. Problematically, autocorrelation in tracking data can result in space needs being severely underestimated. Based on the previous work, we hypothesized the magnitude of underestimation varies with body mass, a relationship that could have serious conservation implications. To evaluate this hypothesis for terrestrial mammals, we estimated home-range areas with global positioning system (GPS) locations from 757 individuals across 61 globally distributed mammalian species with body masses ranging from 0.4 to 4000 kg. We then applied blockcross validation to quantify bias in empirical home range estimates. Area requirements of mammals < 10 kg were underestimated by a mean approximately 15%, and species weighing approximately 100 kg were underestimatedby approximately 50% on average. Thus, we found area estimation was subject to autocorrelation induced bias that was worse for large species. Combined with the fact that extinction risk increases as body mass increases, theallometric scaling of bias we observed suggests the most threatened species are also likely to be those with theleast accurate home range estimates. As a correction, we tested whether data thinning or autocorrelation informedhome range estimation minimized the scaling effect of autocorrelation on area estimates. Data thinning requiredan approximately 93% data loss to achieve statistical independence with 95% confidence and was, therefore, nota viable solution. In contrast, autocorrelation informed home range estimation resulted in consistently accurateestimates irrespective of mass. When relating body mass to home range size, we detected that correcting forautocorrelation resulted in a scaling exponent significantly >1, meaning the scaling of the relationship changedsubstantially at the upper end of the mass spectrum. MenosAccurately quantifying species' area requirements is a prerequisite for effective area-based conservation. This typically involves collecting tracking data on species of interest and then conducting home range analyses. Problematically, autocorrelation in tracking data can result in space needs being severely underestimated. Based on the previous work, we hypothesized the magnitude of underestimation varies with body mass, a relationship that could have serious conservation implications. To evaluate this hypothesis for terrestrial mammals, we estimated home-range areas with global positioning system (GPS) locations from 757 individuals across 61 globally distributed mammalian species with body masses ranging from 0.4 to 4000 kg. We then applied blockcross validation to quantify bias in empirical home range estimates. Area requirements of mammals < 10 kg were underestimated by a mean approximately 15%, and species weighing approximately 100 kg were underestimatedby approximately 50% on average. Thus, we found area estimation was subject to autocorrelation induced bias that was worse for large species. Combined with the fact that extinction risk increases as body mass increases, theallometric scaling of bias we observed suggests the most threatened species are also likely to be those with theleast accurate home range estimates. As a correction, we tested whether data thinning or autocorrelation informedhome range estimation minimized the scaling effect of autocorrelation on ar... Mostrar Tudo |
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Thesagro: |
Comportamento Animal; Conservação; Mamífero. |
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Thesaurus NAL: |
Animal behavior; Conservation status; Home range; Mammals. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/215878/1/BodySizeEstimation2020.pdf
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Marc: |
LEADER 04945naa a2201153 a 4500
001 1792404
005 2020-09-14
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1111/cobi.13495$2DOI
100 1 $aNOONAN, M. J.
245 $aEffects of body size on estimation of mammalian area requirements.
260 $c2020
520 $aAccurately quantifying species' area requirements is a prerequisite for effective area-based conservation. This typically involves collecting tracking data on species of interest and then conducting home range analyses. Problematically, autocorrelation in tracking data can result in space needs being severely underestimated. Based on the previous work, we hypothesized the magnitude of underestimation varies with body mass, a relationship that could have serious conservation implications. To evaluate this hypothesis for terrestrial mammals, we estimated home-range areas with global positioning system (GPS) locations from 757 individuals across 61 globally distributed mammalian species with body masses ranging from 0.4 to 4000 kg. We then applied blockcross validation to quantify bias in empirical home range estimates. Area requirements of mammals < 10 kg were underestimated by a mean approximately 15%, and species weighing approximately 100 kg were underestimatedby approximately 50% on average. Thus, we found area estimation was subject to autocorrelation induced bias that was worse for large species. Combined with the fact that extinction risk increases as body mass increases, theallometric scaling of bias we observed suggests the most threatened species are also likely to be those with theleast accurate home range estimates. As a correction, we tested whether data thinning or autocorrelation informedhome range estimation minimized the scaling effect of autocorrelation on area estimates. Data thinning requiredan approximately 93% data loss to achieve statistical independence with 95% confidence and was, therefore, nota viable solution. In contrast, autocorrelation informed home range estimation resulted in consistently accurateestimates irrespective of mass. When relating body mass to home range size, we detected that correcting forautocorrelation resulted in a scaling exponent significantly >1, meaning the scaling of the relationship changedsubstantially at the upper end of the mass spectrum.
650 $aAnimal behavior
650 $aConservation status
650 $aHome range
650 $aMammals
650 $aComportamento Animal
650 $aConservação
650 $aMamífero
700 1 $aFLEMING, C. H.
700 1 $aTUCKER, M. A.
700 1 $aKAYS, R.
700 1 $aHARRISON, AUTUMN-LYNN
700 1 $aCROFOOT, M. C.
700 1 $aABRAHMS, B.
700 1 $aALBERTS, S.
700 1 $aALI, A. H.
700 1 $aALTMANN, J.
700 1 $aANTUNES, P. C.
700 1 $aATTIAS, N.
700 1 $aBELANT, J. L.
700 1 $aBEYER JUNIOR, D. E.
700 1 $aBIDNER, L. R.
700 1 $aBLAUM, N.
700 1 $aBOONE, R. B.
700 1 $aCAILLAUD, D.
700 1 $aPAULA, R. C. de
700 1 $aDE LA TORRE, J. A.
700 1 $aDEKKER, J.
700 1 $aDEPERNO, C. S.
700 1 $aFARHADINIA, M.
700 1 $aFENNESSY, J.
700 1 $aFICHTEL, C.
700 1 $aFISCHER, C.
700 1 $aFORD, A.
700 1 $aGOHEEN, J. R.
700 1 $aHAVMØLLER, R. W.
700 1 $aHIRSCH, B. T.
700 1 $aHURTADO, C.
700 1 $aISBELL, L. A.
700 1 $aJANSSEN, R.
700 1 $aJELTSCH, F.
700 1 $aKACZENSKY, P.
700 1 $aKANEKO, Y.
700 1 $aKAPPELER, P.
700 1 $aKATNA, A.
700 1 $aKAUFFMAN, M.
700 1 $aKOCH, F.
700 1 $aKULKARNI, A
700 1 $aLAPOINT, S.
700 1 $aLEIMGRUBER, P.
700 1 $aMACDONALD, D. W.
700 1 $aMARKHAM, A. C.
700 1 $aMCMAHON, L.
700 1 $aMERTES, K.
700 1 $aMOORMAN, C. E.
700 1 $aMORATO, R. G.
700 1 $aMOßBRUCKER, A. M.
700 1 $aMOURAO, G.
700 1 $aO'CONNOR, D.
700 1 $aOLIVEIRA-SANTOS, L. G. R.
700 1 $aPASTORINI, J.
700 1 $aPATTERSON, B. D.
700 1 $aRACHLOW, J.
700 1 $aRANGLACK, D. H.
700 1 $aREID, N.
700 1 $aSCANTLEBURY, D. M.
700 1 $aSCOTT, D. M.
700 1 $aSELVA, N.
700 1 $aSERGIEL, A.
700 1 $aSONGER, M.
700 1 $aSONGSASEN, N.
700 1 $aSTABACH, J. A.
700 1 $aSTACY-DAWES, J.
700 1 $aSWINGEN, M. B.
700 1 $aTHOMPSON, J. J.
700 1 $aULLMANN, W.
700 1 $aVANAK, A. T.
700 1 $aTHAKER, M.
700 1 $aWILSON, J. W.
700 1 $aYAMAZAKI, K.
700 1 $aYARNELL, R. W.
700 1 $aZIEBA, F.
700 1 $aZWIJACZ-KOZICA, T.
700 1 $aFAGAN, W. F.
700 1 $aMUELLER, T.
700 1 $aCALABRESE, J. M.
773 $tConservation Biology$gv.34, n. 4, p. 1017-1028, 2020.
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