| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Semiárido. |
|
Data corrente: |
09/02/2021 |
|
Data da última atualização: |
09/02/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SILVA, K. C. D. da; AMORIM, M. C. C. de; GALVÃO, R. S.; GONÇALVES, Y. B. de O.; SILVA, P. T. de S. e; BARROS, E. S. C. |
|
Afiliação: |
Kessia Caroline Dantas da Silva, UNIVASF; Miriam Cleide Cavalcante de Amorim, UNIVASF; Renan Santana Galvão, UNIVASF; Yandra Beatriz de Oliveira Gonçalves, UNIVASF; PAULA TEREZA DE SOUZA E SILVA, CPATSA; Eduardo Souza Costa Barros. |
|
Título: |
Effect of the inoculum/substrate ratio on the biochemical methane potential (BMP) of grape marc. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Revista Ambiente & Agua, v. 15, n. 5, 2020. |
|
DOI: |
10.4136/ambi-agua.2541 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The grape industrialization process produces large volumes of solid organic waste, with the grape bagasse being the main waste generated in the winemaking process. Anaerobic digestion can be used to treat and dispose of agro-industrial biomass waste. The objective of this study was to evaluate the effect of the inoculum/substrate ratio on the Biochemical Methane Potential (BMP) of grape marc. The experiment was performed in laboratory scale through a system of reactor bottles in batches, removing a set of triplicate flasks for sampling and analysis every 48 hours, with the test lasting 12 days. The reactors contained residue, inoculum and 20% of nutritive solution, maintaining 20% of headspace. The reactors were incubated in an incubator at a mesophilic temperature (35 ± 2°C) and shaken manually every 24 hours. Three different inoculum/substrate (I/S) ratios of 0.75, 1.5 and 3 were used to evaluate the methane yield, organic removals and at the end of degradation the morphology of the bacterial community was evaluated by means of scanning electron microscopy. The I/S 3 ratio provided the best results for loading anaerobic systems, indicating that grape marc presents potential for biological treatment through anaerobic digestion. |
|
Palavras-Chave: |
Bagaço da uva; Biometano; Digestão anaeróbica; PBM; Potencial Bioquímico de Metano; Resíduos agroindustriais; Resíduos sólidos orgânicos. |
|
Thesagro: |
Biogás; Resíduo; Resíduo Agrícola; Resíduo Industrial; Uva. |
|
Thesaurus Nal: |
Anaerobic digestion. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/221026/1/Effect-of-the-inoculum-substrate-ratio-on-the-biochemical-methane-potential-BMP-of-grape-marc-2020.pdf
|
|
Marc: |
LEADER 02289naa a2200349 a 4500
001 2129879
005 2021-02-09
008 2020 bl uuuu u00u1 u #d
024 7 $a10.4136/ambi-agua.2541$2DOI
100 1 $aSILVA, K. C. D. da
245 $aEffect of the inoculum/substrate ratio on the biochemical methane potential (BMP) of grape marc.$h[electronic resource]
260 $c2020
520 $aThe grape industrialization process produces large volumes of solid organic waste, with the grape bagasse being the main waste generated in the winemaking process. Anaerobic digestion can be used to treat and dispose of agro-industrial biomass waste. The objective of this study was to evaluate the effect of the inoculum/substrate ratio on the Biochemical Methane Potential (BMP) of grape marc. The experiment was performed in laboratory scale through a system of reactor bottles in batches, removing a set of triplicate flasks for sampling and analysis every 48 hours, with the test lasting 12 days. The reactors contained residue, inoculum and 20% of nutritive solution, maintaining 20% of headspace. The reactors were incubated in an incubator at a mesophilic temperature (35 ± 2°C) and shaken manually every 24 hours. Three different inoculum/substrate (I/S) ratios of 0.75, 1.5 and 3 were used to evaluate the methane yield, organic removals and at the end of degradation the morphology of the bacterial community was evaluated by means of scanning electron microscopy. The I/S 3 ratio provided the best results for loading anaerobic systems, indicating that grape marc presents potential for biological treatment through anaerobic digestion.
650 $aAnaerobic digestion
650 $aBiogás
650 $aResíduo
650 $aResíduo Agrícola
650 $aResíduo Industrial
650 $aUva
653 $aBagaço da uva
653 $aBiometano
653 $aDigestão anaeróbica
653 $aPBM
653 $aPotencial Bioquímico de Metano
653 $aResíduos agroindustriais
653 $aResíduos sólidos orgânicos
700 1 $aAMORIM, M. C. C. de
700 1 $aGALVÃO, R. S.
700 1 $aGONÇALVES, Y. B. de O.
700 1 $aSILVA, P. T. de S. e
700 1 $aBARROS, E. S. C.
773 $tRevista Ambiente & Agua$gv. 15, n. 5, 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Semiárido (CPATSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Unidades Centrais. |
|
Data corrente: |
19/06/2018 |
|
Data da última atualização: |
19/06/2018 |
|
Autoria: |
MOLINARI, P. C. C.; BLAGITZ, M. G.; DELLA LIBERA, A. M. M. P.; BATISTA, C. F.; SOUZA, F. N. |
|
Afiliação: |
Paula C. C. Molinari, Veterinary Clinical Immunology Research Group/DCMD/FMVZ/Universidade de São Paulo; Maiara G. Blagitz, Veterinary Clinical Immunology Research Group/DCMD/FMVZ/Universidade de São Paulo; Alice M. M. P. Della Libera, Veterinary Clinical Immunology Research Group/DCMD/FMVZ/Universidade de São Paulo; Camila F. Batista, Veterinary Clinical Immunology Research Group/DCMD/FMVZ/Universidade de São Paulo; Fernando N. Souza, Veterinary Clinical Immunology Research Group/DCMD/FMVZ/Universidade de São Paulo. |
|
Título: |
Intracellular reactive oxygen species production and phagocytosis of Staphylococcus aureus by milk neutrophils as tool to diagnose mastitis and identify susceptible dairy cows. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 4, p. 659-664, abril 2018 |
|
Idioma: |
Inglês |
|
Notas: |
Título em português: Produção intracelular de espécies reativas de oxigênio e fagocitose de Staphylococcus aureus por neutrófilos lácteos como ferramentas para o diagnóstico da mastite e identificação de vacas leiteiras susceptíveis à mastite. |
|
Conteúdo: |
The immune response capacity of the mammary gland plays a major role to determine if mastitis will or not be established. Thus, we hypothesize that a better understanding of polymorphonuclear neutrophil leukocyte (PMN) function will elucidate mechanisms that will improve our knowledge of how we could avoid an inflammatory process by increasing the immune capacity of the cow, and even further, to search for a tool to diagnose mastitis or a possible way to select and identify non-susceptible animals. The present study utilized 112 quarters from 28 Holstein dairy cows that were divided into quarters milk samples with somatic cell count (SCC) <2×105 cells mL-1 (n=72) and SCC >2×105 cells mL-1 (n=40). The percentages of milk PMNs and the levels of intracellular reactive oxygen species (ROS) and the phagocytosis of Staphylococcus aureus by milk neutrophils were evaluated by flow cytometry. Our results showed a higher percentage of neutrophils in quarter milk samples with high SCC (P=0.0003), and this group also had a significantly higher percentage of neutrophils that produced ROS (P=0.008). On the other hand, the phagocytosis intensity of S. aureus by milk neutrophils was higher in quarters with low SCC (P=0.003), suggesting a better mammary gland immunity against invading pathogens. Analyzing the results of the predictive values of the measured PMN functions, they cannot be used isolated as a good diagnosis test since none of them had a satisfactory sensitivity and specificity values, which was also confirmed by the Youden index values being far from one. In conclusion, the assessment of milk bovine neutrophil functions could improve our understanding of the cellular basis of mastitis. Although, the intracellular ROS production and S. aureus phagocytosis by milk neutrophil did not have high predictive values to detect intramammary infections, our results strengthen the idea that that poor bovine mammary gland neutrophil phagocytic ability may be associated with high SCC, and might be considered to identify susceptible dairy cows to mastitis. MenosThe immune response capacity of the mammary gland plays a major role to determine if mastitis will or not be established. Thus, we hypothesize that a better understanding of polymorphonuclear neutrophil leukocyte (PMN) function will elucidate mechanisms that will improve our knowledge of how we could avoid an inflammatory process by increasing the immune capacity of the cow, and even further, to search for a tool to diagnose mastitis or a possible way to select and identify non-susceptible animals. The present study utilized 112 quarters from 28 Holstein dairy cows that were divided into quarters milk samples with somatic cell count (SCC) <2×105 cells mL-1 (n=72) and SCC >2×105 cells mL-1 (n=40). The percentages of milk PMNs and the levels of intracellular reactive oxygen species (ROS) and the phagocytosis of Staphylococcus aureus by milk neutrophils were evaluated by flow cytometry. Our results showed a higher percentage of neutrophils in quarter milk samples with high SCC (P=0.0003), and this group also had a significantly higher percentage of neutrophils that produced ROS (P=0.008). On the other hand, the phagocytosis intensity of S. aureus by milk neutrophils was higher in quarters with low SCC (P=0.003), suggesting a better mammary gland immunity against invading pathogens. Analyzing the results of the predictive values of the measured PMN functions, they cannot be used isolated as a good diagnosis test since none of them had a satisfactory sensitivity and specificity v... Mostrar Tudo |
|
Palavras-Chave: |
Especificidade; Infecção intramamária; Intracellular reactive; Intramammary infection; Milk neutrophils; Saúde da glândula mamária; Sensibilidade; Sensitivity; Udder health status. |
|
Thesagro: |
Bacteriose; Oxigênio; Vaca Leiteira. |
|
Thesaurus NAL: |
Cattle; Dairy cows; Immune response; Mastitis; Oxygen production; Phagocytosis. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178768/1/Intracellular-reactive-oxygen-species.pdf
|
|
Marc: |
LEADER 03573naa a2200397 a 4500
001 2092602
005 2018-06-19
008 2018 bl uuuu u00u1 u #d
100 1 $aMOLINARI, P. C. C.
245 $aIntracellular reactive oxygen species production and phagocytosis of Staphylococcus aureus by milk neutrophils as tool to diagnose mastitis and identify susceptible dairy cows.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Produção intracelular de espécies reativas de oxigênio e fagocitose de Staphylococcus aureus por neutrófilos lácteos como ferramentas para o diagnóstico da mastite e identificação de vacas leiteiras susceptíveis à mastite.
520 $aThe immune response capacity of the mammary gland plays a major role to determine if mastitis will or not be established. Thus, we hypothesize that a better understanding of polymorphonuclear neutrophil leukocyte (PMN) function will elucidate mechanisms that will improve our knowledge of how we could avoid an inflammatory process by increasing the immune capacity of the cow, and even further, to search for a tool to diagnose mastitis or a possible way to select and identify non-susceptible animals. The present study utilized 112 quarters from 28 Holstein dairy cows that were divided into quarters milk samples with somatic cell count (SCC) <2×105 cells mL-1 (n=72) and SCC >2×105 cells mL-1 (n=40). The percentages of milk PMNs and the levels of intracellular reactive oxygen species (ROS) and the phagocytosis of Staphylococcus aureus by milk neutrophils were evaluated by flow cytometry. Our results showed a higher percentage of neutrophils in quarter milk samples with high SCC (P=0.0003), and this group also had a significantly higher percentage of neutrophils that produced ROS (P=0.008). On the other hand, the phagocytosis intensity of S. aureus by milk neutrophils was higher in quarters with low SCC (P=0.003), suggesting a better mammary gland immunity against invading pathogens. Analyzing the results of the predictive values of the measured PMN functions, they cannot be used isolated as a good diagnosis test since none of them had a satisfactory sensitivity and specificity values, which was also confirmed by the Youden index values being far from one. In conclusion, the assessment of milk bovine neutrophil functions could improve our understanding of the cellular basis of mastitis. Although, the intracellular ROS production and S. aureus phagocytosis by milk neutrophil did not have high predictive values to detect intramammary infections, our results strengthen the idea that that poor bovine mammary gland neutrophil phagocytic ability may be associated with high SCC, and might be considered to identify susceptible dairy cows to mastitis.
650 $aCattle
650 $aDairy cows
650 $aImmune response
650 $aMastitis
650 $aOxygen production
650 $aPhagocytosis
650 $aBacteriose
650 $aOxigênio
650 $aVaca Leiteira
653 $aEspecificidade
653 $aInfecção intramamária
653 $aIntracellular reactive
653 $aIntramammary infection
653 $aMilk neutrophils
653 $aSaúde da glândula mamária
653 $aSensibilidade
653 $aSensitivity
653 $aUdder health status
700 1 $aBLAGITZ, M. G.
700 1 $aDELLA LIBERA, A. M. M. P.
700 1 $aBATISTA, C. F.
700 1 $aSOUZA, F. N.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 4, p. 659-664, abril 2018
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
