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| 9. |  | LIRA, A. C.; MASCARIN, G. M.; DELALIBERA JUNIOR, I. Produção de microescleródios de Metarhizium spp. (Hypocreales: Clavicipitaceae) por fermentação com duas fontes de nitrogênio. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 27.; CONGRESSO LATINO-AMERICANO DE ENTOMOLOGIA, 10., 2018, Gramado. Saúde, ambiente e agricultura: anais... Gramado, DF: SEB: UFSM, 2018. p. 314.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 12. | | MASCARIN, G. M.; DUARTE, V. da S.; BRANDÃO, M. M.; DELALIBERA JUNIOR, I. Natural occurrence of Zoophthora radicans (Entomophthorales: Entomophthoraceae) on Thaumastocoris peregrinus (Heteroptera: Thaumastocoridae), an invasive pest recently found in Brazil. Journal of Invertebrate Pathology, New York, v. 110, n. 3, p. 401-404, Jul. 2012.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 16. | | MASCARIN, G. M.; KOBORI, N. N.; JACKSON, M. A.; DUNLAP, C. A.; DELALIBERA JUNIOR, I. Nitrogen sources affect productivity, desiccation tolerance and storage stability of Beauveria bassiana blastospores. Journal of Applied Microbiology, v. 124, n. 3, p. 810-820, 2018.| Biblioteca(s): Embrapa Meio Ambiente. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
30/05/2016 |
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Data da última atualização: |
21/02/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MASCARIN, G. M.; JACKSON, M. A.; BEHLE, R. W.; KOBORI, N. N.; DELALIBERA JÚNIOR, I. |
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Afiliação: |
GABRIEL MOURA MASCARIN, CNPAF; MARK A. JACKSON, USDA; ROBERT W. BEHLE, USDA; NILCE N. KOBORI, USDA; ITALO DELALIBERA JUNIOR, ESALQ. |
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Título: |
Improved shelf life of dried Beauveria bassiana blastospores using convective drying and active packaging processes. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Applied Microbiology and Biotechnology, Berlin, v. 100, n. 19, p. 8359-8370, Oct. 2016. |
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DOI: |
10.1007/s00253-016-7597-2 |
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Idioma: |
Inglês |
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Notas: |
Published online: 20 May 2016. |
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Conteúdo: |
The yeast form (blastospore) of the dimorphic insect-pathogenic fungus Beauveria bassiana can be rapidly produced using liquid fermentation methods but is generally unable to survive rapid dehydration processes or storage under non-refrigerated conditions. In this study, we evaluated the influence of two convective drying methods, various modified atmosphere packaging systems, and storage temperatures on the desiccation tolerance, storage stability, and virulence of blastospores of B. bassiana ESALQ 1432. All blastospore formulations were dried to <5 % water content equivalent to aw < 0.3. The viability of B. bassiana blastospores after air drying and spray drying was greater than 80 %. Vacuum-packaged blastospores remained viable longer when stored at 4 °C compared with 28 °C with virtually no loss in viability over 9 months regardless the drying method. When both oxygen and moisture scavengers were added to sealed packages of dried blastospore formulations stored at 28 °C, viability was significantly prolonged for both air- and spraydried blastospores. The addition of ascorbic acid during spray drying did not improve desiccation tolerance but enhanced cell stability (∼twofold higher half-life) when stored at 28 °C. After storage for 4 months at 28 °C, air-dried blastospores produced a lower LC80 and resulted in higher mortality to whitefly nymphs (Bemisia tabaci) when compared with spray-dried blastospores. These studies identified key storage conditions (low aw and oxygen availability) that improved blastospore storage stability at 28 °C and will facilitate the commercial development of blastospores-based bioinsecticides. MenosThe yeast form (blastospore) of the dimorphic insect-pathogenic fungus Beauveria bassiana can be rapidly produced using liquid fermentation methods but is generally unable to survive rapid dehydration processes or storage under non-refrigerated conditions. In this study, we evaluated the influence of two convective drying methods, various modified atmosphere packaging systems, and storage temperatures on the desiccation tolerance, storage stability, and virulence of blastospores of B. bassiana ESALQ 1432. All blastospore formulations were dried to <5 % water content equivalent to aw < 0.3. The viability of B. bassiana blastospores after air drying and spray drying was greater than 80 %. Vacuum-packaged blastospores remained viable longer when stored at 4 °C compared with 28 °C with virtually no loss in viability over 9 months regardless the drying method. When both oxygen and moisture scavengers were added to sealed packages of dried blastospore formulations stored at 28 °C, viability was significantly prolonged for both air- and spraydried blastospores. The addition of ascorbic acid during spray drying did not improve desiccation tolerance but enhanced cell stability (∼twofold higher half-life) when stored at 28 °C. After storage for 4 months at 28 °C, air-dried blastospores produced a lower LC80 and resulted in higher mortality to whitefly nymphs (Bemisia tabaci) when compared with spray-dried blastospores. These studies identified key storage conditions (low aw and ... Mostrar Tudo |
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Palavras-Chave: |
Liquid fermentation; Storage stability. |
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Thesagro: |
Beauveria bassiana; Bemisia tabaci; Dessecante; Fungo; Mosca branca; Secagem. |
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Thesaurus NAL: |
Air drying; Desiccants; Free radical scavengers; Mycoinsecticides; Spray drying. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 02737naa a2200349 a 4500
001 2045820
005 2017-02-21
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1007/s00253-016-7597-2$2DOI
100 1 $aMASCARIN, G. M.
245 $aImproved shelf life of dried Beauveria bassiana blastospores using convective drying and active packaging processes.$h[electronic resource]
260 $c2016
500 $aPublished online: 20 May 2016.
520 $aThe yeast form (blastospore) of the dimorphic insect-pathogenic fungus Beauveria bassiana can be rapidly produced using liquid fermentation methods but is generally unable to survive rapid dehydration processes or storage under non-refrigerated conditions. In this study, we evaluated the influence of two convective drying methods, various modified atmosphere packaging systems, and storage temperatures on the desiccation tolerance, storage stability, and virulence of blastospores of B. bassiana ESALQ 1432. All blastospore formulations were dried to <5 % water content equivalent to aw < 0.3. The viability of B. bassiana blastospores after air drying and spray drying was greater than 80 %. Vacuum-packaged blastospores remained viable longer when stored at 4 °C compared with 28 °C with virtually no loss in viability over 9 months regardless the drying method. When both oxygen and moisture scavengers were added to sealed packages of dried blastospore formulations stored at 28 °C, viability was significantly prolonged for both air- and spraydried blastospores. The addition of ascorbic acid during spray drying did not improve desiccation tolerance but enhanced cell stability (∼twofold higher half-life) when stored at 28 °C. After storage for 4 months at 28 °C, air-dried blastospores produced a lower LC80 and resulted in higher mortality to whitefly nymphs (Bemisia tabaci) when compared with spray-dried blastospores. These studies identified key storage conditions (low aw and oxygen availability) that improved blastospore storage stability at 28 °C and will facilitate the commercial development of blastospores-based bioinsecticides.
650 $aAir drying
650 $aDesiccants
650 $aFree radical scavengers
650 $aMycoinsecticides
650 $aSpray drying
650 $aBeauveria bassiana
650 $aBemisia tabaci
650 $aDessecante
650 $aFungo
650 $aMosca branca
650 $aSecagem
653 $aLiquid fermentation
653 $aStorage stability
700 1 $aJACKSON, M. A.
700 1 $aBEHLE, R. W.
700 1 $aKOBORI, N. N.
700 1 $aDELALIBERA JÚNIOR, I.
773 $tApplied Microbiology and Biotechnology, Berlin$gv. 100, n. 19, p. 8359-8370, Oct. 2016.
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