Data/hora: 14/06/2024 02:14:52

Biblioteca(s): | Embrapa Meio Ambiente.
Data corrente: | 06/10/2021
Data da última atualização: | 10/06/2022
Autoria: | IWANICKI, N. S.; MASCARIN, G. M.; MORENO, S. G.; EILENBERG, J.; DELALIBERA JUNIOR, I.
Afiliação: | NATASHA SANT´ANNA IWANICKI, ESALQ-USP; GABRIEL MOURA MASCARIN, CNPMA; SARA GIRO MORENO, ESALQ-USP; JØRGEN EILENBERG, University of Copenhagen; ITALO DELALIBERA JUNIOR, ESALQ-USP.
Título: | Development of novel spray-dried and air-dried formulations of Metarhizium robertsii blastospores and their virulence.
Ano de publicação: | 2021
Fonte/Imprenta: | Applied Microbiology and Biotechnology, v. 105, n. 20, p. 7913-7933, 2021.
ISSN: | 1432-0614
DOI: | https://doi.org/10.1007/s00253-021-11576-5
Idioma: | Inglês
Conteúdo: | Abstract: The present research addressed spray-drying and air-drying techniques applied to Metarhizium robertsii blastospores to develop wettable powder (WP) formulations. We investigated the effect of co-formulants on blastospore viability during drying and assessed the wettability and stability of formulations in water. The effect of oxygen-moisture absorbers was studied on the shelf life of these formulations stored at 26 °C and 4 °C for up to 90 days. Additionally, we determined the virulence of the best spray-dried and air-dried formulations against the corn leafhopper Dalbulus maidis. While sucrose and skim milk played an essential role as osmoprotectants in preserving air-dried blastospores, maltodextrin, skim milk, and bentonite were crucial to attain high cell survival during spray drying. The lowest wettability time was achieved with spray-dried formulations containing less Ca-lignin, while charcoal powder amount was positively associated with formulation stability. The addition of oxygen-moisture absorbers inside sealed packages increased from threefold to fourfold the half-life times of air-dried and spray-dried formulations at both storage temperatures. However, the half-life times of all blastospore-based formulations were shorter than 3 months regardless of temperature and packaging system. Spray-dried and air-dried WP formulations were as virulent as fresh blastopores against D. maydis adults sprayed with 5&#8201;×&#8201;107 blastospores mL&#8722;1 that induced 87.8% and 70.6% mortality, respectively. These findings bring innovative advancement for M. robertsii blastospore formulation through spray-drying and underpin the importance of adding protective matrices coupled to oxygen-moisture absorbers to extend cell viability during either cold or non-refrigerated storage.
Palavras-Chave: | Convective drying.
Thesagro: | Cigarrinha; Fermentação; Formulação; Fungo Entomógeno; Fungo Para Controle Biológico; Pó Molhável; Praga de Planta.
Thesaurus Nal: | Biological control agents; Dalbulus maidis; Entomopathogenic fungi; Liquid state fermentation; Metarhizium robertsii; Pesticide formulations; Wettable powders.
Categoria do assunto: | H Saúde e Patologia
Registro original: | Embrapa Meio Ambiente (CNPMA)
Exemplares: | CNPMA - (17036-1) - UPC - AP - DD -  -  -  - DIS