|
|
Registros recuperados : 66 | |
62. | | OLIVEIRA, Y. de; ADAMUCHIO, L. G.; OLIVEIRA, C. de; DEGENHARDT-GOLDBACH, J.; GERHARDT, I.; BESPALHOK FILHO, J. C.; DIBAZ, R.; QUOIRIN, M. Indirect organogenesis from leaf explants of Eucalyptus benthamii x Eucalyptus dunnii and shoot multiplication. In: IUFRO TREE BIOTECHNOLOGY CONFERENCE, 2011, Arraial d'Ajuda. From genomes do integration and delivery: extended abstracts proceedings. [S.l.]: Embrapa: Veracel: IUFRO, 2011. 1 CD-ROM. Biblioteca(s): Embrapa Florestas. |
| |
63. | | PELEGRINI, L. L.; LEANDRO F. de OLIVEIRA; FERREIRA, D. L.; PADILHA, J. H. D.; SILVA, R. C. da; MATTIA, R. C.; DEGENHARDT-GOLDBACH, J.; QUOIRIN, M. Indução de calogênese em explantes foliares de Calophyllum brasiliense. In: CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 18.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 5., 2011, Joinville. Resumos... Joinville: Sociedade Brasileira de Plantas Ornamentais e Associação Brasileira de Cultura de Tecidos de Plantas, 2011. Biblioteca(s): Embrapa Florestas. |
| |
64. | | BETTENCOURT, G. M. de F.; SOCCOL, C. R.; GIOVANELLA, T. S.; FRANCISCON, L.; KESTRING, D. R.; GERHARDT, I. R.; DEGENHARDT-GOLDBACH, J. Agrobacterium tumefaciens-mediated transformation of Eucalyptus urophylla clone BRS07-01. Journal of Forestry Research, v. 31, n. 2, p. 507-519, Apr. 2020. Biblioteca(s): Embrapa Agricultura Digital; Embrapa Florestas. |
| |
65. | | TOMASI, J. de C.; DEGENHARDT-GOLDBACH, J.; GRUNENNVALDT, R. L.; SANTOS, G. D. dos; VICENTE, V. A.; FRANCISCON, L.; BONA, C.; QUOIRIN, M. In vitro establishment of shoot meristems of Ilex paraguariensis and identification of endophytic bacteria. Journal of Forestry Research, v. 30, n. 5, p. 1765-1777, Oct. 2019. Biblioteca(s): Embrapa Florestas. |
| |
66. | | OLIVEIRA, P. R. D. de; RITSCHEL, P. S.; RUFATO, A. De R.; FIORAVANÇO, J. C.; FAORO, I. D.; LEITE, G. B.; DUTRA, L. F.; MAYER, N. A.; DEGENHARDT-GOLDBACH, J. The Brazilian pear breeding program. Acta Horticulturae, The Hague, n. 909, p. 145-151, out. 2011. Trabalho apresentado no 11º International Pear Symposium. Biblioteca(s): Embrapa Florestas; Embrapa Uva e Vinho. |
| |
Registros recuperados : 66 | |
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Florestas. |
Data corrente: |
13/11/2019 |
Data da última atualização: |
13/03/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
BETTENCOURT, G. M. de F.; SOCCOL, C. R.; GIOVANELLA, T. S.; FRANCISCON, L.; KESTRING, D. R.; GERHARDT, I. R.; DEGENHARDT-GOLDBACH, J. |
Afiliação: |
Gisela Manuela de França Bettencourt, UFPR; Carlos Ricardo Soccol, UFPR; Thais Salete Giovanella, UFPR; LUZIANE FRANCISCON, CNPF; DAIANE RIGONI, CNPF; ISABEL RODRIGUES GERHARDT, CNPTIA; JULIANA DEGENHARDT GOLDBACH, CNPF. |
Título: |
Agrobacterium tumefaciens-mediated transformation of Eucalyptus urophylla clone BRS07-01. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Journal of Forestry Research, v. 31, n. 2, p. 507-519, Apr. 2020. |
DOI: |
10.1007/s11676-018-0777-4 |
Idioma: |
Inglês |
Conteúdo: |
Abstract Genetic transformation is becoming routine for engineering specific traits in important clones of recalcitrant species such as Eucalyptus; however, the efficiency is still low for most species, so many researchers still use seeds instead of clones as initial explants. This work aimed to develop a genetic transformation protocol, based on a highly efficient in vitro organogenesis protocol, for an Eucalyptus urophylla clone selected in our breeding program. Plant growth regulators were evaluated for indirect organogenesis and rooting. In a two-step protocol, the combination of callus induction media supplemented with 0.5 lM thidiazuron + 0.5 lM naphthaleneacetic acid (NAA) and shoot induction media supplemented with 5.0 lM benzylaminopurine + 1.0 lM NAA allowed up to 85.6% shoot formation with more shoots per explants when compared with other concentrations. Transgenic plants expressing the uidA gene were obtained using Agrobacterium tumefaciens and selected for kanamycin resistance. A RAPD analysis was used to check for somaclonal variation. In tests using 11 RAPD primers, we did not observe somaclonal variation in the in vitro stages evaluated. |
Palavras-Chave: |
Plant growth regulators; Regeneração in vitro; Reguladores de crescimento de plantas; Transformação genética. |
Thesagro: |
Eucalipto; Eucalyptus Urophylla. |
Thesaurus NAL: |
Genetic transformation; In vitro regeneration; Somaclonal variation. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02181naa a2200313 a 4500 001 2114265 005 2020-03-13 008 2020 bl uuuu u00u1 u #d 024 7 $a10.1007/s11676-018-0777-4$2DOI 100 1 $aBETTENCOURT, G. M. de F. 245 $aAgrobacterium tumefaciens-mediated transformation of Eucalyptus urophylla clone BRS07-01.$h[electronic resource] 260 $c2020 520 $aAbstract Genetic transformation is becoming routine for engineering specific traits in important clones of recalcitrant species such as Eucalyptus; however, the efficiency is still low for most species, so many researchers still use seeds instead of clones as initial explants. This work aimed to develop a genetic transformation protocol, based on a highly efficient in vitro organogenesis protocol, for an Eucalyptus urophylla clone selected in our breeding program. Plant growth regulators were evaluated for indirect organogenesis and rooting. In a two-step protocol, the combination of callus induction media supplemented with 0.5 lM thidiazuron + 0.5 lM naphthaleneacetic acid (NAA) and shoot induction media supplemented with 5.0 lM benzylaminopurine + 1.0 lM NAA allowed up to 85.6% shoot formation with more shoots per explants when compared with other concentrations. Transgenic plants expressing the uidA gene were obtained using Agrobacterium tumefaciens and selected for kanamycin resistance. A RAPD analysis was used to check for somaclonal variation. In tests using 11 RAPD primers, we did not observe somaclonal variation in the in vitro stages evaluated. 650 $aGenetic transformation 650 $aIn vitro regeneration 650 $aSomaclonal variation 650 $aEucalipto 650 $aEucalyptus Urophylla 653 $aPlant growth regulators 653 $aRegeneração in vitro 653 $aReguladores de crescimento de plantas 653 $aTransformação genética 700 1 $aSOCCOL, C. R. 700 1 $aGIOVANELLA, T. S. 700 1 $aFRANCISCON, L. 700 1 $aKESTRING, D. R. 700 1 $aGERHARDT, I. R. 700 1 $aDEGENHARDT-GOLDBACH, J. 773 $tJournal of Forestry Research$gv. 31, n. 2, p. 507-519, Apr. 2020.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Florestas (CNPF) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|