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| Registros recuperados : 5 | |
| 1. |  | DEDICOVA, B.; BERMUDEZ, C.; PRIAS, M.; ZUNIGA, E.; BRONDANI, C. High-throughput transformation pipeline for a Brazilian japonica rice with bar gene selection. Protoplasma, New York, v. 252, n. 4, p. 1071-1083, Jul. 2015. Published online: 07 December 2014.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 2. | | CRUZ, T. I. da; ROCHA, D. C.; LANNA, A. C.; DEDICOVA, B.; VIANELLO, R. P.; BRONDANI, C. Calcium-dependent protein kinase 5 (OsCPK5) overexpression in upland rice (Oryza sativa L.) under water deficit. Plants, v. 12, n. 22, 3826, Nov. 2023.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 4. | | ABREU, F. R. M.; DEDICOVA, B.; VIANELLO, R. P.; LANNA, A. C.; OLIVEIRA, J. A. V. de; VIEIRA, A. F.; MORAIS, O. P.; MENDONÇA, J. A.; BRONDANI, C. Overexpression of a phospholipase (OsPLDa1) for drought tolerance in upland rice (Oryza sativa L.). Protoplasma, v. 255, n. 6, p. 1751-1761, Nov. 2018.| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 5. | | ROCHA, D. C.; CRUZ, T. I. da; OLIVEIRA, J. A. V. DE; SOUZA, I. P. DE; DEDICOVA, B.; COELHO, A. S. G.; VIANELLO, R. P.; BRONDANI, C. Physiological and phenotypical effects of the overexpression of the OVP1 gene in Japonica rice. Pesquisa Agropecuária Brasileira, v. 58, e03167, 2023. Título em português: Efeitos fisiológicos e fenotípicos da superexpressão do gene OVP1 em arroz japonica.| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Unidades Centrais. |
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| Registros recuperados : 5 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
08/12/2014 |
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Data da última atualização: |
16/01/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
DEDICOVA, B.; BERMUDEZ, C.; PRIAS, M.; ZUNIGA, E.; BRONDANI, C. |
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Afiliação: |
BEATA DEDICOVA, CIAT; C. BERMUDEZ, CIAT; M. PRIAS, CIAT; E. ZUNIGA, CIAT; CLAUDIO BRONDANI, CNPAF. |
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Título: |
High-throughput transformation pipeline for a Brazilian japonica rice with bar gene selection. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Protoplasma, New York, v. 252, n. 4, p. 1071-1083, Jul. 2015. |
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DOI: |
10.1007/s00709-014-0741-x |
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Idioma: |
Inglês |
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Notas: |
Published online: 07 December 2014. |
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Conteúdo: |
The goal of this work was to establish a transformation pipeline for upland Curinga rice (Oryza sativa L. ssp. japonica) with bar gene selection employing bialaphos and phosphinothricin as selection agents. The following genes of interest: AtNCED3, Lsi1, GLU2, LEW2, PLD-alpha, DA1, TOR, AVP1, and Rubisco were cloned into the binary vector p7i2x-Ubi and were transferred into Agrobacterium strain EHA 105. Embryogenic calli derived from the mature embryos were transformed, and transgenic cells and shoots were selected on the medium supplemented with bialaphos or phosphinothricin (PPT) using a stepwise selection scheme. Molecular analyses were established using polymerase chain reaction and Southern blot for the bar gene and the NOS terminator. Overall, 273 putative transgenic plants were analyzed by Southern blot with 134 events identified. In total, 77 events had a single copy of the transgene integrated in the plant genome while 29 events had two copies. We tested backbone integration in 101 transgenic plants from all constructs and found 60 transgenic plants having no additional sequence integrated in the plant genome. The bar gene activity was evaluated by the chlorophenol red test and the leaf painting test using phosphinothricin with several transgenic plants. The majority of T0 plants carrying the single copy of transgene produced T1 seeds in the screen house. |
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Palavras-Chave: |
Bar selection. |
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Thesagro: |
Arroz; Oryza sativa; Planta transgênica. |
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Thesaurus NAL: |
Agrobacterium; Genetic transformation; Pipelines; Rice. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02230naa a2200289 a 4500
001 2001907
005 2018-01-16
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1007/s00709-014-0741-x$2DOI
100 1 $aDEDICOVA, B.
245 $aHigh-throughput transformation pipeline for a Brazilian japonica rice with bar gene selection.$h[electronic resource]
260 $c2015
500 $aPublished online: 07 December 2014.
520 $aThe goal of this work was to establish a transformation pipeline for upland Curinga rice (Oryza sativa L. ssp. japonica) with bar gene selection employing bialaphos and phosphinothricin as selection agents. The following genes of interest: AtNCED3, Lsi1, GLU2, LEW2, PLD-alpha, DA1, TOR, AVP1, and Rubisco were cloned into the binary vector p7i2x-Ubi and were transferred into Agrobacterium strain EHA 105. Embryogenic calli derived from the mature embryos were transformed, and transgenic cells and shoots were selected on the medium supplemented with bialaphos or phosphinothricin (PPT) using a stepwise selection scheme. Molecular analyses were established using polymerase chain reaction and Southern blot for the bar gene and the NOS terminator. Overall, 273 putative transgenic plants were analyzed by Southern blot with 134 events identified. In total, 77 events had a single copy of the transgene integrated in the plant genome while 29 events had two copies. We tested backbone integration in 101 transgenic plants from all constructs and found 60 transgenic plants having no additional sequence integrated in the plant genome. The bar gene activity was evaluated by the chlorophenol red test and the leaf painting test using phosphinothricin with several transgenic plants. The majority of T0 plants carrying the single copy of transgene produced T1 seeds in the screen house.
650 $aAgrobacterium
650 $aGenetic transformation
650 $aPipelines
650 $aRice
650 $aArroz
650 $aOryza sativa
650 $aPlanta transgênica
653 $aBar selection
700 1 $aBERMUDEZ, C.
700 1 $aPRIAS, M.
700 1 $aZUNIGA, E.
700 1 $aBRONDANI, C.
773 $tProtoplasma, New York$gv. 252, n. 4, p. 1071-1083, Jul. 2015.
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Embrapa Arroz e Feijão (CNPAF) |
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