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| Registros recuperados : 96 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
01/04/2022 |
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Data da última atualização: |
01/04/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PILLAI, V. V.; KEI, T. G.; GURUNG, S.; DAS, M.; SIQUEIRA, L. G. B.; CHEONG, S. H.; HANSEN, P. J.; SELVARAJ, V. |
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Afiliação: |
VIJU VIJAYAN PILLAI, Cornell University; TIFFANY G. KEI, Cornell University; SHAILESH GURUNG, Cornell University; MOUBANI DAS, Cornell University; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; SOON HON CHEONG, Cornell University; PETER J. HANSEN, University of Florida; VIMAL SELVARAJ, Cornell University. |
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Título: |
RhoA/ROCK signaling antagonizes bovine trophoblast stem cell self-renewal and regulates preimplantation embryo size and differentiation. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Development, v. 149, n. 7, dev200115, 2022. |
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DOI: |
https://doi.org/10.1242/dev.200115 |
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Idioma: |
Inglês |
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Conteúdo: |
Exponential proliferation of trophoblast stem cells (TSC) is crucial in Ruminantia to maximize numerical access to caruncles, the restricted uterine sites that permit implantation. When translating systems biology of the undifferentiated bovine trophectoderm, we uncovered that inhibition of RhoA/Rock promoted self-renewing proliferation and substantially increased blastocyst size. Analysis of transcripts suppressed by Rock inhibition revealed transforming growth factor β1 (TGFβ1) as a primary upstream effector. TGFβ1 treatment induced changes consistent with differentiation in bTSCs, a response that could be replicated by induced expression of the bovine ROCK2 transgene. Rocki could partially antagonize TGFβ1 effects, and TGFβ receptor inhibition promoted proliferation identical to Rocki, indicating an all-encompassing upstream regulation. Morphological differentiation included formation of binucleate cells and infrequent multinucleate syncytia, features we also localize in the in vivo bovine placenta. Collectively, we demonstrate a central role for TGFβ1, RhoA and Rock in inducing bTSC differentiation, attenuation of which is sufficient to sustain self-renewal and proliferation linked to blastocyst size and preimplantation development. Unraveling these mechanisms augments evolutionary/comparative physiology of the trophoblast cell lineage and placental development in eutherians. |
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Palavras-Chave: |
Blastocisto; Células-tronco; Trofoblasto. |
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Thesagro: |
Bovino; Embrião Animal; Reprodução Animal. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02295naa a2200289 a 4500
001 2141687
005 2022-04-01
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1242/dev.200115$2DOI
100 1 $aPILLAI, V. V.
245 $aRhoA/ROCK signaling antagonizes bovine trophoblast stem cell self-renewal and regulates preimplantation embryo size and differentiation.$h[electronic resource]
260 $c2022
520 $aExponential proliferation of trophoblast stem cells (TSC) is crucial in Ruminantia to maximize numerical access to caruncles, the restricted uterine sites that permit implantation. When translating systems biology of the undifferentiated bovine trophectoderm, we uncovered that inhibition of RhoA/Rock promoted self-renewing proliferation and substantially increased blastocyst size. Analysis of transcripts suppressed by Rock inhibition revealed transforming growth factor β1 (TGFβ1) as a primary upstream effector. TGFβ1 treatment induced changes consistent with differentiation in bTSCs, a response that could be replicated by induced expression of the bovine ROCK2 transgene. Rocki could partially antagonize TGFβ1 effects, and TGFβ receptor inhibition promoted proliferation identical to Rocki, indicating an all-encompassing upstream regulation. Morphological differentiation included formation of binucleate cells and infrequent multinucleate syncytia, features we also localize in the in vivo bovine placenta. Collectively, we demonstrate a central role for TGFβ1, RhoA and Rock in inducing bTSC differentiation, attenuation of which is sufficient to sustain self-renewal and proliferation linked to blastocyst size and preimplantation development. Unraveling these mechanisms augments evolutionary/comparative physiology of the trophoblast cell lineage and placental development in eutherians.
650 $aBovino
650 $aEmbrião Animal
650 $aReprodução Animal
653 $aBlastocisto
653 $aCélulas-tronco
653 $aTrofoblasto
700 1 $aKEI, T. G.
700 1 $aGURUNG, S.
700 1 $aDAS, M.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aCHEONG, S. H.
700 1 $aHANSEN, P. J.
700 1 $aSELVARAJ, V.
773 $tDevelopment$gv. 149, n. 7, dev200115, 2022.
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