Data/hora: 05/06/2024 02:27:29

Biblioteca(s): 	Embrapa Suínos e Aves.	
Data corrente: 	12/08/2022	
Data da última atualização: 	12/08/2022	
Autoria: 	IBELLI, A. M. G.; PEIXOTO, J. de O.; ZANELLA, R.; GOUVEIA, J. J. de S.; CANTAO, M. E.; COUTINHO, L. L.; MARCHESI, J. A. P.; DAL PIZZOL, M. S.; MARCELINO, D. E. P.; LEDUR, M. C.	
Afiliação: 	ADRIANA MERCIA GUARATINI IBELLI, CNPSA; JANE DE OLIVEIRA PEIXOTO, CNPSA; RICARDO ZANELLA, UPF; JOÃO JOSÉ DE SIMONI GOUVEIA, UNIVASF; MAURICIO EGIDIO CANTAO, CNPSA; LUIZ LEHMANN COUTINHO, Esalq; JORGE AUGUSTO PETROLI MARCHESI, USP; MARIANE SPUDEIT DAL PIZZOL, UDESC/Chapecó; DÉBORA ESTER PETRY MARCELINO, FACC; MONICA CORREA LEDUR, CNPSA.	
Título: 	Downregulation of growth plate genes involved with the onset of femoral head separation in young broilers.	
Ano de publicação: 	2022	
Fonte/Imprenta: 	Frontiers in Physiology, v. 3, n. 941134, 2022. doi: 10.3389/fphys.2022.941134	
DOI: 	https://doi.org/10.3389/fphys.2022.941134	
Idioma: 	Inglês	
Conteúdo: 	Abstract: Femoral head separation (FHS) is characterized by the detachment of growth plate (GP) and articular cartilage, occurring in tibia and femur. However, the molecular mechanisms involved with this condition are not completely understood. Therefore, genes and biological processes (BP) involved with FHS were identified in 21-day-old broilers through RNA sequencing of the femoral GP. 13,487 genes were expressed in the chicken femoral head transcriptome of normal and FHS-affected broilers. From those, 34 were differentially expressed (DE; FDR &#8804;0.05) between groups, where all of them were downregulated in FHS-affected broilers. The main BP were enriched in receptor signaling pathways, ossification, bone mineralization and formation, skeletal morphogenesis, and vascularization. RNA-Seq datasets comparison of normal and FHS-affected broilers with 21, 35 and 42 days of age has shown three shared DE genes (FBN2, C1QTNF8, and XYLT1) in GP among ages. Twelve genes were exclusively DE at 21 days, where 10 have already been characterized (SHISA3, FNDC1, ANGPTL7, LEPR, ENSGALG00000049529, OXTR, ENSGALG00000045154, COL16A1, RASD2, BOC, GDF10, and THSD7B). Twelve SNPs were associated with FHS (p < 0.0001). Out of those, 5 were novel and 7 were existing variants located in 7 genes (RARS, TFPI2, TTI1, MAP4K3, LINK54, and AREL1). We have shown that genes related to chondrogenesis and bone differentiation were downregulated in the GP of FHS-affected young broilers. Therefore, these findings evince that candidate genes pointed out in our study are probably related to the onset of FHS in broilers.	
Palavras-Chave: 	FBN2; Femoral head necrosis; FHS; Necrose da cabeça femoral; RNA-Seq.	
Thesagro: 	Frango; Galinha; Genética Animal.	
Thesaurus Nal: 	Chickens; Gene expression.	
Categoria do assunto: 	--	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1145416/1/final9937.pdf	
Registro original: 	Embrapa Suínos e Aves (CNPSA)	
Exemplares: 	CNPSA - (22397-1) - UPC - AP - DD -  -  -  - DIS