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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
12/09/2022 |
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Data da última atualização: |
12/04/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ANGELO, P. C. da S.; PEREIRA, L. F. P.; SERA, G. H.; SETA, T. |
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Afiliação: |
PAULA CRISTINA DA SILVA ANGELO, CNPCa. |
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Título: |
Coffee microspore cultivation to attain doubled-haploid stable plantlets. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
INTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França. |
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DOI: |
10.17660/ActaHortic.2023.1359.14 |
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Idioma: |
Inglês |
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Notas: |
E-poster. |
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Conteúdo: |
The aim of this study was to explore the potentials of progenies produced by the introgression of disease resistance from related Coffea species to C. arabica . In the IDR-Parana, Brazil, hybrids of C. arabica (tetraploid) x C. racemosa (diploid) were obtained. Self-pollination of some of these hybrids produced very mild yields, despite the backcros to C. arabica . In an attempt to regenerate stable double-haploids, anthers and isolated microspores were grown in vitro . It was assumed that chromosomes captured in a microspore after meiosis can acquire homo and/or homeologous stability faster in vitro than in vivo. C. arabica anthers served as control. Flowers were collected when the young microspores were uninucleated, and disinfected with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer that worked for a few seconds, washed and centrifuged twice at 100xg and grown in modified N6 liquid medium (10 5 cells/mL in 35 mm diameter plates). Explants were grown continuously for six months on the same N6 medium containing 6.5 mg/L auxins, 1.0 mg/L cytokinins and 0.5 mg/L gibberellin (GA3), at 27 o C in the dark. Isolated microspores produced embryo-like structures or microcalli at very low frequencies (0.3 per plate). The anthers, on the other hand, produced embryogenic calli . One particular endogenous fungus infected 70% of the control anthers but 5% produced friable calli . Some of these were embryogenic, but arrested when compared to calli produced by the interspecific hybrid anthers. Regarding the hybrid anthers, 25% were infected by a same endogenous bacteria but 8% produced embryogenic tissue, with globular embryos multiplying and maturing simultaneously upon transfer to N6 medium without growth regulators. After 1.2 years, the photomorphogenesis has been induced under light, and auxin/cytokinin ratio = 2 plus GA3. MenosThe aim of this study was to explore the potentials of progenies produced by the introgression of disease resistance from related Coffea species to C. arabica . In the IDR-Parana, Brazil, hybrids of C. arabica (tetraploid) x C. racemosa (diploid) were obtained. Self-pollination of some of these hybrids produced very mild yields, despite the backcros to C. arabica . In an attempt to regenerate stable double-haploids, anthers and isolated microspores were grown in vitro . It was assumed that chromosomes captured in a microspore after meiosis can acquire homo and/or homeologous stability faster in vitro than in vivo. C. arabica anthers served as control. Flowers were collected when the young microspores were uninucleated, and disinfected with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer that worked for a few seconds, washed and centrifuged twice at 100xg and grown in modified N6 liquid medium (10 5 cells/mL in 35 mm diameter plates). Explants were grown continuously for six months on the same N6 medium containing 6.5 mg/L auxins, 1.0 mg/L cytokinins and 0.5 mg/L gibberellin (GA3), at 27 o C in the dark. Isolated microspores produced embryo-like structures or microcalli at very low frequencies (0.3 per plate). The anthers, on the other hand, produced embryogenic calli . One particular endogenous fungus infected 70% of the control anthers but 5% produced friable calli . Some of these were embryogenic, but arrested when compared to calli prod... Mostrar Tudo |
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Thesagro: |
Café; Embriogénese; Pólen; Rubiaceae. |
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Thesaurus Nal: |
Embryogenesis; Pollen. |
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Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/249059/1/Coffee-microspore-cultivation.pdf
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Marc: |
LEADER 02577nam a2200241 a 4500
001 2146372
005 2023-04-12
008 2022 bl uuuu u00u1 u #d
024 7 $a10.17660/ActaHortic.2023.1359.14$2DOI
100 1 $aANGELO, P. C. da S.
245 $aCoffee microspore cultivation to attain doubled-haploid stable plantlets.$h[electronic resource]
260 $aINTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França.$c2022
500 $aE-poster.
520 $aThe aim of this study was to explore the potentials of progenies produced by the introgression of disease resistance from related Coffea species to C. arabica . In the IDR-Parana, Brazil, hybrids of C. arabica (tetraploid) x C. racemosa (diploid) were obtained. Self-pollination of some of these hybrids produced very mild yields, despite the backcros to C. arabica . In an attempt to regenerate stable double-haploids, anthers and isolated microspores were grown in vitro . It was assumed that chromosomes captured in a microspore after meiosis can acquire homo and/or homeologous stability faster in vitro than in vivo. C. arabica anthers served as control. Flowers were collected when the young microspores were uninucleated, and disinfected with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer that worked for a few seconds, washed and centrifuged twice at 100xg and grown in modified N6 liquid medium (10 5 cells/mL in 35 mm diameter plates). Explants were grown continuously for six months on the same N6 medium containing 6.5 mg/L auxins, 1.0 mg/L cytokinins and 0.5 mg/L gibberellin (GA3), at 27 o C in the dark. Isolated microspores produced embryo-like structures or microcalli at very low frequencies (0.3 per plate). The anthers, on the other hand, produced embryogenic calli . One particular endogenous fungus infected 70% of the control anthers but 5% produced friable calli . Some of these were embryogenic, but arrested when compared to calli produced by the interspecific hybrid anthers. Regarding the hybrid anthers, 25% were infected by a same endogenous bacteria but 8% produced embryogenic tissue, with globular embryos multiplying and maturing simultaneously upon transfer to N6 medium without growth regulators. After 1.2 years, the photomorphogenesis has been induced under light, and auxin/cytokinin ratio = 2 plus GA3.
650 $aEmbryogenesis
650 $aPollen
650 $aCafé
650 $aEmbriogénese
650 $aPólen
650 $aRubiaceae
700 1 $aPEREIRA, L. F. P.
700 1 $aSERA, G. H.
700 1 $aSETA, T.
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Registro original: |
Embrapa Café (CNPCa) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
24/09/2002 |
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Data da última atualização: |
21/03/2019 |
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Autoria: |
ROSA, I. V.; CARVALHO, J. C.; HOUSER, R. H.; DOBEREINER, J. |
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Afiliação: |
IVAN VALADÃO ROSA, EMBRAPA/Centro Nacional de Pesquisa de Gado de Corte; JOSÉ CARLOS CARVALHO, Secretaria de Agricultura do Estado de Mato Grosso; RICHARD H. HOUSER, University of Florida; JURGEN DOBEREINER, EMBRAPA/RJ. Setor de Anatomia Patológica, Patologia Animal. |
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Título: |
Influência de ração balanceada sobre a ?cara inchada? (doença peridentária) de bezerros. |
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Ano de publicação: |
1976 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 11, n. 9, p. 59-63, 1976. |
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Série: |
(Veterinária). |
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Idioma: |
Português |
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Notas: |
Título em inglês: Effect of barn feeding on "Cara inchada" (periodontal disease) in calves. |
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Conteúdo: |
Dois lotes de 6 e 10 bezerros desmamados, afetados por lesões peridentárias da "cara inchada", foram confinados em duas fazendas, na região de Rondonópolis, Mato Grosso; durante 5 a 6 meses os animais receberam exclusivamente ração balanceada, adquirida fora da fazenda. Observou-se rápida melhora do estado de nutrição destes animais e houve regressão e reparação das lesões peridentárias. Nos animais de controle, mantidos em pastos de P. maximum nas mesmas duas fazendas, as lesões peridentárias persistiram ou agravaram-se e vários animais morreram. 0 experimento confirma conclusões, às quais se chegou em trabalhos anteriores, de que a 1~ cara inchada- deve ser causada por fator alimentar existente em certas áreas onde pastejam os bovinos. |
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Palavras-Chave: |
bezerros; bovinos; influência de ração balanceada; peridentite. |
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Thesagro: |
Cara Inchada; Doença Peridentária. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/194806/1/Influencia-de-racao-balanceada.pdf
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Marc: |
LEADER 01589naa a2200253 a 4500
001 1108555
005 2019-03-21
008 1976 bl uuuu u00u1 u #d
100 1 $aROSA, I. V.
245 $aInfluência de ração balanceada sobre a ?cara inchada? (doença peridentária) de bezerros.
260 $c1976
490 $a(Veterinária).
500 $aTítulo em inglês: Effect of barn feeding on "Cara inchada" (periodontal disease) in calves.
520 $aDois lotes de 6 e 10 bezerros desmamados, afetados por lesões peridentárias da "cara inchada", foram confinados em duas fazendas, na região de Rondonópolis, Mato Grosso; durante 5 a 6 meses os animais receberam exclusivamente ração balanceada, adquirida fora da fazenda. Observou-se rápida melhora do estado de nutrição destes animais e houve regressão e reparação das lesões peridentárias. Nos animais de controle, mantidos em pastos de P. maximum nas mesmas duas fazendas, as lesões peridentárias persistiram ou agravaram-se e vários animais morreram. 0 experimento confirma conclusões, às quais se chegou em trabalhos anteriores, de que a 1~ cara inchada- deve ser causada por fator alimentar existente em certas áreas onde pastejam os bovinos.
650 $aCara Inchada
650 $aDoença Peridentária
653 $abezerros
653 $abovinos
653 $ainfluência de ração balanceada
653 $aperidentite
700 1 $aCARVALHO, J. C.
700 1 $aHOUSER, R. H.
700 1 $aDOBEREINER, J.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 11, n. 9, p. 59-63, 1976.
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Embrapa Unidades Centrais (AI-SEDE) |
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