Data/hora: 08/06/2024 02:40:16

Biblioteca(s): | Embrapa Solos.
Tipo da produção científica: | Artigo em Periódico Indexado
Data corrente: | 08/10/2021
Data da última atualização: | 08/10/2021
Autoria: | ELMERICH, C.; QUIVIGER, B.; ROSENBERG, C.; FRANCHE, C.; LAURENT, P.; DOBEREINER, J.
Afiliação: | CLAUDINE ELMERICH, INSTITUT PASTEUR, PARIS; BERNADETTE QUIVIGER, INSTITUT PASTEUR, PARIS; CHARLES ROSENBERG, LABORATOIRE DE BIOLOGIE MOLECULAIRE DES RELATIONS PLANTES MICROORGANISMES; CLAUDINE FRANCHE, INSTITUT PASTEUR, PARIS; PATRICK LAURENT, INSTITUT PASTEUR, PARIS; JOHANNA DOBEREINER, SNLCS.
Título: | Characterization of a temperate bacteriophage for Azospirillum.
Ano de publicação: | 1982
Fonte/Imprenta: | Virology, v. 122, n. 1, p. 29-37, Oct. 1982.
DOI: | https://doi.org/10.1016/0042-6822(82)90374-9
Idioma: | Inglês
Conteúdo: | A temperate bacteriophage, forming plaques on Azospirillum lipoferum strain Br17, was isolated from Brazilian soil samples. The phage morphology as revealed by electron microscopy showed an icosahedral head of 56 nm and a tail of 250 nm, to which six spikes are attached. The phage genome is double-stranded linear DNA of 36 kb with cohesive ends. Its host range is limited to strains of Brazilian origin, three being A. lipoferum and one A. brasilense. Spontaneous mutant phages having altered plaque morphology were isolated with a frequency of 10-4. Stable lysogens were obtained with all sensitive strains and shown to contain the prophage DNA maintained as a plasmid. Restriction analysis of the linear phage DNA and of the circular prophage provided proof of their identity and allowed identification of the restriction fragments carrying the cohesive ends.
Thesaurus Nal: | Azospirillum.
Categoria do assunto: | P Recursos Naturais, Ciências Ambientais e da Terra
Registro original: | Embrapa Solos (CNPS)
Exemplares: | CNPS - (20820-1) - UPC - AP - DD -  -  -  - DIS