Data/hora: 14/06/2024 01:51:06

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 04/03/2016
Data da última atualização: | 04/03/2016
Autoria: | BORSANELLI, A. C.; GAETTI-JARDIM JUNIOR, E.; DOBEREINER, J.; DUTRA, I. S.
Afiliação: | ANA CAROLINA BORSANELLI, UNESP; ELERSON GAETTI-JARDIM JÚNIOR, UNESP; JURGEN DOBEREINER; IVERALDO S. DUTRA, UNESP.
Título: | Treponema denticola in microflora of bovine periodontitis.
Ano de publicação: | 2015
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 3, p. 237-240, mar. 2015.
Idioma: | Inglês
Conteúdo: | Periodontitis in cattle is an infectious purulent progressive disease associated with strict anaerobic subgingival biofilm and is epidemiologically related to soil management at several locations of Brazil. This study aimed to detect Treponema species in periodontal pockets of cattle with lesions deeper than 5mm in the gingival sulcus of 6 to 24-month-old animals considered periodontally healthy. We used paper cones to collect the materials, after removal of supragingival plaques, and kept frozen (at -80°C) up to DNA extraction and polymerase chain reaction (PCR) using T. amylovorum, T. denticola, T. maltophilum, T. medium and T. vincentii primers. In periodontal pocket, it was possible to identify by PCR directly, the presence of Treponema amylovorum in 73% of animals (19/26), T. denticola in 42.3% (11/26) and T. maltophilum in 54% (14/26). Among the 25 healthy sites, it was possible to identify T. amylovorum in 18 (72%), T. denticola in two (8%) and T. maltophilum in eight (32%). Treponema medium and T. vincentii were not detected over all 51 evaluated samples. The presence of Treponema amylovorum, T. maltophilum and, in particular, the widely recognized T. denticola in subgingival microflora brings an original and potencially important contribution in studies of the bovine periodontitis. INDEX TERMS: Bovine periodontitis, periodontal disease, subgingival microflora, Treponema denticola.
Palavras-Chave: | Bovine periodontitis; Doença periodontal; Microbiota subgengival; Periodontite bovina; Subgtingival microflora.
Thesaurus Nal: | Periodontal diseases; Treponema denticola.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/140641/1/Treponema-denticola-in-microflora.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (59067-1) - UPE - AP - PP - 636.08905 - P474 -  - DIS