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| Registros recuperados : 14 | |
| 3. |  | CUNHA, N. B. da; MURAD, A. M.; VIANNA, G. R.; RECH FILHO, E. L. Recombinant biosynthesis of functional human growth hormone and coagulation factor IX in transgenic soybean seeds. BMC Proceedings, v.8, suppl, P.112, 2014. (Open access) Edição do Congress of the Brazilian Biotechnology Society, Florianópolis, 2013.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | LACORTE, C. C.; FERREIRA, A. L.; MURAD, A. M.; CUNHA, N. B. da; PINHEIRO, P. V. Seed-based production of recombinant proteins. In: KOLE, C.; CHAURASIA, A.; HEFFERON, K. L.; PANIGRAHI, J. (ed.). Tools & techniques of plant molecular farming. [S.l.]: Springer Singapore, 2023. Cap. 6. Cap. 6, p. 185-208.| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | CUNHA, N. B. da; LEITE, M. L.; COSTA, L. S. L.; CUNHA, V. A.; DIAS, S. C.; VIANNA, G. R.; RECH, E. L. Basic aspects for choosing crops for recombinant production of therapeutic and industrial molecules. The International Journal of Engineering and Science (IJES), II, v. 7, n. 8, p. 51-56, 2018.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | CUNHA, N. B. da; LEITE, M. L.; COSTA, L. S. L.; CUNHA, V. A.; DIAS, S. C.; VIANNA, G. R.; RECH, E. L. An overview of the early development of the first genetically engineered crop- derived foods The International Journal of Engineering and Science (IJES), Series II, v. 8, n. 12, p. 46-50, 2019.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | MURAD, A. M.; VIANNA, G. R.; MACHADO, A. M.; CUNHA, N. B. da; COELHO, C. M.; LACERDA, V. A. M.; COELHO, M. C.; RECH FILHO, E. L. Mass spectrometry characterisation of fatty acids from metabolically engineered soybean seeds. Analytical and Bioanalytical Chemistry, v. 406, n. 12, p. 2873-2883, 2014.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | CAMPOS, M. L.; PRADO, G. S.; SANTOS, V. O. dos; NASCIMENTO, L. C.; DOHMS, S. M.; CUNHA, N. B. da; RAMADA, M. H. S.; GROSSI-DE-SA, M. F.; DIAS, S. C. Mosses: versatile plants for biotechnological applications. Biotechnology Advances, v. 41, article 107533, 2020.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | FARIA, J. C.; ALBINO, M. M. C.; DIAS, B. B. A.; CANÇADO, L. J.; CUNHA, N. B. da; SILVA, L. de M.; VIANNA, G. R.; ARAGÃO, F. J. L. Partial resistance to Bean golden mosaic virus in a transgenic common bean (Phaseolus vulgaris L.) line expressing a mutated rep gene. Plant Science, Limerick, v. 171, p. 565-571, 2006.| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | O'KEEFE, B. R.; MURAD, A. M.; VIANNA, G. R.; RAMESSAR, K.; SAUCEDO, C. J.; WILSON, J.; BUCKHEIT, K. W.; CUNHA, N. B. da; ARAUJO, A. C. G.; LACORTE, C. C.; MADEIRA, L.; MCMAHON, J. B.; RECH, E. L. Engineering soya bean seeds as a scalable platform to produce cyanovirin-N, a non-ARV microbicide against HIV. Plant Biotechnology Journal, p. 1-9, 2015.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | GÓMEZ, M. L.; HUANG, X.; ALVAREZ, D.; HE, W.; BAYSAL, C.; ZHU, C.; ARMARIO-NAJERA, V.; PERERA, A. B.; BENNASSER, P. C.; SABA-MAYORAL, A.; SOBRINO-MENGUAL, G.; VARGHEESE, A.; ABRANCHES, R.; ABREU, I. A.; BALAMURUGAN, S.; BOCK, R.; BUYEL, J. F.; CUNHA, N. B. da; DANIELL, H.; FALLER, R.; FOLGADO, A.; GOWTHAM, I.; HÄKKINEN, S. T.; KUMAR, S.; RAMALINGAM, S. K.; LACORTE, C. C.; LOMONOSSOFF, G. P.; LUÍS, I. M.; MA, J. K.-C.; MCDONALD, K. A.; MURAD, A. M.; NANDI, S.; O'KEEFE, B.; OKSMAN-CALDENTEY, K.-M.; PARTHIBAN, S.; PAUL, M. J.; PONNDORF, D.; RECH FILHO, E. L.; RODRIGUES, J. C. M.; RUF, S.; SCHILLBERG, S.; SCHWESTKA, J.; SHAH, P. S.; SINGH, R.; STOGER, E.; TWYMAN, R. M.; VARGHESE, I. P.; VIANNA, G. R.; WEBSTER, G.; WILBERS, R. H. P.; CAPELL, T.; CHRISTOU, P. Contributions of the international plant science community to the fight against human infectious diseases - part 1: epidemic and pandemic diseases. Plant Biotechnology Journal, v. 19, p. 1901-1920, 2021. Na publicação: Cristiano Lacorte; Andre Murad; Elibio Rech.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | HE, W.; BAYSAL, C.; GÓMEZ, M. L.; HUANG, X.; ALVAREZ, D.; ZHU, C.; ARMARIO-NAJERA, V.; PERERA, A. B.; BENNASER, P. C.; SABA-MAYORAL, A.; SOBRINO-MENGUAL, G.; VARGHEESE, A.; ABRANCHES, R.; ABREU, I. A.; BALAMURUGAN, S.; BOCK, R.; BUYEL, J. F.; CUNHA, N. B. da; DANIELL, H.; FALLER, R.; FOLGADO, A.; GOWTHAM, I.; HÄKKINEN, S. T.; KUMAR, S.; KUMAR, R. S.; LACORTE, C. C.; LOMONOSSOFF, G. P.; LUÍS, I. M.; MA, J. K.-C.; MCDONALD, K. A.; MURAD, A. M.; NANDI, S.; O’KEEF, B.; PARTHIBAN, S.; PAUL, M. J.; PONNDORF, D.; RECH FILHO, E. L.; RODRIGUES, J. C. M.; RUF, S.; SCHILLBERG, S.; SCHWESTKA, J.; SHAH, P. S.; SINGH, R.; STOGER, E.; TWYMAN, R. M.; VARGHESE, I. P.; VIANNA, G. R.; WEBSTER, G.; WILBERS, R. H. P.; CHRISTOU, P.; OKSMAN-CALDENTEY, K.-M.; CAPELL, T. Contributions of the international plant science community to the fight against infectious diseases in humans - part 2: Affordable drugs in edible plants for endemic and re-emerging diseases. Plant Biotechnology Journal, v. 19, p. 1921-1936, 2021. Na publicação: Cristiano Lacorte; Andre Murad; Elibio Rech.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 14 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
10/11/2006 |
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Data da última atualização: |
03/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
-- - A |
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Autoria: |
FARIA, J. C.; ALBINO, M. M. C.; DIAS, B. B. A.; CANÇADO, L. J.; CUNHA, N. B. da; SILVA, L. de M.; VIANNA, G. R.; ARAGÃO, F. J. L. |
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Título: |
Partial resistance to Bean golden mosaic virus in a transgenic common bean (Phaseolus vulgaris L.) line expressing a mutated rep gene. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Plant Science, Limerick, v. 171, p. 565-571, 2006. |
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Idioma: |
Inglês |
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Conteúdo: |
The rep gene of Bean golden mosaic virus (BGMV) is essential for virus replication. A mutated rep gene with amino acid codon change in the putative nucleoside triphosphate (NTP) binding motif D262R was created. Phaseolus vulgaris transformation was achieved with a vector that contained the mutated rep and bar genes. A total of 17 initial (T0) transformants were analyzed. One line (M1/4) showed tolerance to glufosinate ammonium and partial resistance to the virus, that is, disease incidence depended on inoculation level. The incidence of BGMVincreased with the increasing number of viruliferous whiteflies per plant, both in the transgenic and in the control plants. However, the number of symptomless plants was significantly higher in the transgenic group. The line M1/4 was studied during several generations and presented stability in the transgene loci and virus resistance. Southern blot analysis with genomic DNA of eight generations led to an estimate of two copies of the rep gene integrated at the same locus. RT-PCR analysis revealed the presence of both bar and rep genes transcripts. The mutated REP protein was present in amounts detectable by Western blot analysis in transgenic plants. |
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Palavras-Chave: |
BGMV; Dry bean; Geminivirus; Rep gene transdominance. |
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Thesagro: |
Feijão; Phaseolus Vulgaris; Planta Transgênica; Resistência; Variedade Resistente; Vírus. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178023/1/ID-27510-1.pdf
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Marc: |
LEADER 02135naa a2200325 a 4500
001 1187707
005 2022-06-03
008 2006 bl uuuu u00u1 u #d
100 1 $aFARIA, J. C.
245 $aPartial resistance to Bean golden mosaic virus in a transgenic common bean (Phaseolus vulgaris L.) line expressing a mutated rep gene.
260 $c2006
520 $aThe rep gene of Bean golden mosaic virus (BGMV) is essential for virus replication. A mutated rep gene with amino acid codon change in the putative nucleoside triphosphate (NTP) binding motif D262R was created. Phaseolus vulgaris transformation was achieved with a vector that contained the mutated rep and bar genes. A total of 17 initial (T0) transformants were analyzed. One line (M1/4) showed tolerance to glufosinate ammonium and partial resistance to the virus, that is, disease incidence depended on inoculation level. The incidence of BGMVincreased with the increasing number of viruliferous whiteflies per plant, both in the transgenic and in the control plants. However, the number of symptomless plants was significantly higher in the transgenic group. The line M1/4 was studied during several generations and presented stability in the transgene loci and virus resistance. Southern blot analysis with genomic DNA of eight generations led to an estimate of two copies of the rep gene integrated at the same locus. RT-PCR analysis revealed the presence of both bar and rep genes transcripts. The mutated REP protein was present in amounts detectable by Western blot analysis in transgenic plants.
650 $aFeijão
650 $aPhaseolus Vulgaris
650 $aPlanta Transgênica
650 $aResistência
650 $aVariedade Resistente
650 $aVírus
653 $aBGMV
653 $aDry bean
653 $aGeminivirus
653 $aRep gene transdominance
700 1 $aALBINO, M. M. C.
700 1 $aDIAS, B. B. A.
700 1 $aCANÇADO, L. J.
700 1 $aCUNHA, N. B. da
700 1 $aSILVA, L. de M.
700 1 $aVIANNA, G. R.
700 1 $aARAGÃO, F. J. L.
773 $tPlant Science, Limerick$gv. 171, p. 565-571, 2006.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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