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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
17/12/2015 |
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Data da última atualização: |
05/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
KUMA, K. M.; LOPES-CAITAR, V. S.; ROMERO, C. C. T.; SILVA, S. M. H.; CARVALHO, M. C. C. G.; ABDELNOOR, R. V.; DIAS, W. P.; MARCELINO-GUIMARÃES, F. C. |
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Afiliação: |
UEL; UEL; CNPSo; UEL; UENP; RICARDO VILELA ABDELNOOR, CNPSO; WALDIR PEREIRA DIAS, CNPSO; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO. |
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Título: |
A high efficient protocol for soybean root transformation by Agrobacterium rhizogenes and most stable reference genes for RT-qPCR analysis. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plant Cell Reports, v. 34, n. 11, p. 1987-2000, 2015. |
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ISSN: |
1432-203X |
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DOI: |
10.1007/s00299-015-1845-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Gene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis in composite plants, mainly under nematode infection. MenosGene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis i... Mostrar Tudo |
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Thesagro: |
Soja. |
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Categoria do assunto: |
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Marc: |
LEADER 02326naa a2200241 a 4500
001 2031887
005 2017-08-05
008 2015 bl uuuu u00u1 u #d
022 $a1432-203X
024 7 $a10.1007/s00299-015-1845-2$2DOI
100 1 $aKUMA, K. M.
245 $aA high efficient protocol for soybean root transformation by Agrobacterium rhizogenes and most stable reference genes for RT-qPCR analysis.$h[electronic resource]
260 $c2015
520 $aGene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis in composite plants, mainly under nematode infection.
650 $aSoja
700 1 $aLOPES-CAITAR, V. S.
700 1 $aROMERO, C. C. T.
700 1 $aSILVA, S. M. H.
700 1 $aCARVALHO, M. C. C. G.
700 1 $aABDELNOOR, R. V.
700 1 $aDIAS, W. P.
700 1 $aMARCELINO-GUIMARÃES, F. C.
773 $tPlant Cell Reports$gv. 34, n. 11, p. 1987-2000, 2015.
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca |
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Origem |
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Classificação |
Cutter |
Registro |
Volume |
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URL |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
29/05/2022 |
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Data da última atualização: |
26/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 4 |
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Autoria: |
FILIPPI, A. C. G.; GUARNIERI, P.; CUNHA, C. A. da; WANDER, A. E. |
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Afiliação: |
AMANDA CRISTINA GABAN FILIPPI, UNB; PATRICIA GUARNIERI, UNB; CLEYZER ADRIAN DA CUNHA, UFG; ALCIDO ELENOR WANDER, CNPAF. |
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Título: |
The logic of collective action for rural warehouse condominiums. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Logistics, v. 6, n. 1, 9, Mar. 2022. |
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ISSN: |
2305-6290 |
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DOI: |
https://doi.org/10.3390/logistics6010009 |
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Idioma: |
Inglês |
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Conteúdo: |
Given several bottlenecks in Brazil in distribution logistics, mainly in transport and warehouse activities, some new forms of collective action have appeared. The Condominiums of Rural Warehouses was conceived of to overcome these bottlenecks and provide better income and competitiveness to small producers in agribusiness. This article aims to analyse aspects of collective action with the focus of Rural Condominiums in the context of Brazilian agribusiness. Methods: We conducted exploratory, descriptive and qualitative research under the Theory of Logic of Collective Action lens for this purpose. Besides conducting a literature review, we conducted a semi-structured interview with the managers of the Rural Warehouse Condominiums. We analysed the data through a Categorial Content Analysis. Results: The main results show an approximation of the rural model of the Condominiums of Rural Warehouses with the Theory of Logic of Collective Action, mainly for small producers. Conclusions: We highlight the feasibility of the warehouse structure collectively, as it strengthens and provides greater efficiency to rural business and producers, inserts and integrates the industry into a competitive market environment, provides economic and social benefits, leads to cost reduction, and increased profit. The economic, social and logistical determinants show the product?s commercialisation, logistical gains, and the producers' association regarding the development and growth of rural collective action. This paper can be helpful for practitioners and researchers interested in this field. MenosGiven several bottlenecks in Brazil in distribution logistics, mainly in transport and warehouse activities, some new forms of collective action have appeared. The Condominiums of Rural Warehouses was conceived of to overcome these bottlenecks and provide better income and competitiveness to small producers in agribusiness. This article aims to analyse aspects of collective action with the focus of Rural Condominiums in the context of Brazilian agribusiness. Methods: We conducted exploratory, descriptive and qualitative research under the Theory of Logic of Collective Action lens for this purpose. Besides conducting a literature review, we conducted a semi-structured interview with the managers of the Rural Warehouse Condominiums. We analysed the data through a Categorial Content Analysis. Results: The main results show an approximation of the rural model of the Condominiums of Rural Warehouses with the Theory of Logic of Collective Action, mainly for small producers. Conclusions: We highlight the feasibility of the warehouse structure collectively, as it strengthens and provides greater efficiency to rural business and producers, inserts and integrates the industry into a competitive market environment, provides economic and social benefits, leads to cost reduction, and increased profit. The economic, social and logistical determinants show the product?s commercialisation, logistical gains, and the producers' association regarding the development and growth of rural collect... Mostrar Tudo |
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Palavras-Chave: |
Collective action theory of logic; Rural warehouse condominiums. |
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Thesagro: |
Desenvolvimento Rural. |
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Thesaurus NAL: |
Agribusiness; Collective action; Small businesses; Warehouses. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1143534/1/logistics-2022.pdf
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Marc: |
LEADER 02383naa a2200265 a 4500
001 2143534
005 2022-06-26
008 2022 bl uuuu u00u1 u #d
022 $a2305-6290
024 7 $ahttps://doi.org/10.3390/logistics6010009$2DOI
100 1 $aFILIPPI, A. C. G.
245 $aThe logic of collective action for rural warehouse condominiums.$h[electronic resource]
260 $c2022
520 $aGiven several bottlenecks in Brazil in distribution logistics, mainly in transport and warehouse activities, some new forms of collective action have appeared. The Condominiums of Rural Warehouses was conceived of to overcome these bottlenecks and provide better income and competitiveness to small producers in agribusiness. This article aims to analyse aspects of collective action with the focus of Rural Condominiums in the context of Brazilian agribusiness. Methods: We conducted exploratory, descriptive and qualitative research under the Theory of Logic of Collective Action lens for this purpose. Besides conducting a literature review, we conducted a semi-structured interview with the managers of the Rural Warehouse Condominiums. We analysed the data through a Categorial Content Analysis. Results: The main results show an approximation of the rural model of the Condominiums of Rural Warehouses with the Theory of Logic of Collective Action, mainly for small producers. Conclusions: We highlight the feasibility of the warehouse structure collectively, as it strengthens and provides greater efficiency to rural business and producers, inserts and integrates the industry into a competitive market environment, provides economic and social benefits, leads to cost reduction, and increased profit. The economic, social and logistical determinants show the product?s commercialisation, logistical gains, and the producers' association regarding the development and growth of rural collective action. This paper can be helpful for practitioners and researchers interested in this field.
650 $aAgribusiness
650 $aCollective action
650 $aSmall businesses
650 $aWarehouses
650 $aDesenvolvimento Rural
653 $aCollective action theory of logic
653 $aRural warehouse condominiums
700 1 $aGUARNIERI, P.
700 1 $aCUNHA, C. A. da
700 1 $aWANDER, A. E.
773 $tLogistics$gv. 6, n. 1, 9, Mar. 2022.
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Embrapa Arroz e Feijão (CNPAF) |
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