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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
04/09/2015 |
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Data da última atualização: |
16/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FONSECA, F. C. de A.; FIRMINO, A. A. P.; MACEDO. L. L. P. de; COELHO, R. R.; SOUSA JÚNIOR, J. D. A. de; SILVA JUNIOR, O. B.; TOGAWA, R. C.; PAPPAS JUNIOR, G. J.; GÓIS, L. A. B. de; SILVA, M. C. M. da; SA, M. F. G. de. |
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Afiliação: |
FERNANDO CAMPOS DE ASSIS FONSECA, UnB; ALEXANDRE AUGUSTO PEREIRA FIRMINO, UnB; LEONARDO LIMA PEPINO DE MACEDO, UnB; ROBERTA RAMOS COELHO; JOSÉ DIJAIR ANTONINO DE SOUSA JÚNIOR, UnB; ORZENIL BONFIM SILVA-JUNIOR, UnB; ROBERTO COITI TOGAWA, CENARGEN; GEORGIOS JOANNIS PAPPAS JUNIOR, Unb; LUIZ AVELAR BRANDÃO DE GÓIS, USINA TRUNFO, ALAGOAS; MARIA CRISTINA MATTAR DA SILVA, CENARGEN; MARIA FATIMA GROSSI DE SA, CENARGEN. |
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Título: |
Sugarcane giant borer transcriptome analysis and identification of genes related to digestion. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plos One, fev., 2015. |
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DOI: |
10.1371/journal.pone.0118231 |
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Idioma: |
Inglês |
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Conteúdo: |
Sugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the Gen-Bank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB) transcriptome, a of aminopeptidase N (APN) cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST database for the species. Transcriptome analysis will certainly be useful to identify novel developmental genes, to better understand the insect?s biology and to guide the development of new strategies for insect-pest control. MenosSugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the Gen-Bank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB) transcriptome, a of aminopeptidase N (APN) cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST d... Mostrar Tudo |
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Thesagro: |
Broca Gigante; Controle Biológico; Praga. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/129096/1/journal.pone.0118231.pdf
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Marc: |
LEADER 02579naa a2200289 a 4500
001 2023363
005 2023-03-16
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0118231$2DOI
100 1 $aFONSECA, F. C. de A.
245 $aSugarcane giant borer transcriptome analysis and identification of genes related to digestion.$h[electronic resource]
260 $c2015
520 $aSugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the Gen-Bank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB) transcriptome, a of aminopeptidase N (APN) cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST database for the species. Transcriptome analysis will certainly be useful to identify novel developmental genes, to better understand the insect?s biology and to guide the development of new strategies for insect-pest control.
650 $aBroca Gigante
650 $aControle Biológico
650 $aPraga
700 1 $aFIRMINO, A. A. P.
700 1 $aMACEDO. L. L. P. de
700 1 $aCOELHO, R. R.
700 1 $aSOUSA JÚNIOR, J. D. A. de
700 1 $aSILVA JUNIOR, O. B.
700 1 $aTOGAWA, R. C.
700 1 $aPAPPAS JUNIOR, G. J.
700 1 $aGÓIS, L. A. B. de
700 1 $aSILVA, M. C. M. da
700 1 $aSA, M. F. G. de
773 $tPlos One, fev., 2015.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
25/10/2010 |
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Data da última atualização: |
27/01/2020 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
CRUZ, S. A. B. da; MONTEIRO, A. M. V.; SANTOS, R. |
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Afiliação: |
SERGIO APARECIDO BRAGA DA CRUZ, CNPTIA; ANTONIO MIGUEL VIEIRA MONTEIRO, INPE; RAFAEL SANTOS, INPE. |
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Título: |
Desenvolvimento de aplicações em geoprocessamento usando composição automatizada de serviços geográfcos. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: SIMPÓSIO DE GEOTECNOLOGIAS NO PANTANAL, 3., 2010, Cáceres, MT. Anais... Campinas: Embrapa Informática Agropecuária; São José dos Campos: INPE, 2010. |
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Páginas: |
p. 910-921. |
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ISBN: |
978-85-60424-05-4 |
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Idioma: |
Português |
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Notas: |
Geopantanal 2010. |
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Conteúdo: |
Este trabalho apresenta uma abordagem para construção de aplicações em geoprocessamento através da composição automatizada de Web Services geográfcos. O problema de composição é modelado como um problema de planejamento condicional onde, regras semânticas de requisitos de qualidade de geodados são utilizadas como uma indicação de condições de uso de um dado por uma funcionalidade de geoprocessamento. Esta abordagem permite a criação de composições de serviços mais robustas, confáveis e capazes de produzir geodados de melhor qualidade. |
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Palavras-Chave: |
Conditional planning; Geodata quality; Geographic Web Services; Geoprocessamento; Geoprocessing; Planejamento condicional; Qualidade de geodado; Web Services geográfcos. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/23264/1/p329.pdf
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Marc: |
LEADER 01509nam a2200265 a 4500
001 1865086
005 2020-01-27
008 2010 bl uuuu u00u1 u #d
020 $a978-85-60424-05-4
100 1 $aCRUZ, S. A. B. da
245 $aDesenvolvimento de aplicações em geoprocessamento usando composição automatizada de serviços geográfcos.$h[electronic resource]
260 $aIn: SIMPÓSIO DE GEOTECNOLOGIAS NO PANTANAL, 3., 2010, Cáceres, MT. Anais... Campinas: Embrapa Informática Agropecuária; São José dos Campos: INPE$c2010
300 $ap. 910-921.
500 $aGeopantanal 2010.
520 $aEste trabalho apresenta uma abordagem para construção de aplicações em geoprocessamento através da composição automatizada de Web Services geográfcos. O problema de composição é modelado como um problema de planejamento condicional onde, regras semânticas de requisitos de qualidade de geodados são utilizadas como uma indicação de condições de uso de um dado por uma funcionalidade de geoprocessamento. Esta abordagem permite a criação de composições de serviços mais robustas, confáveis e capazes de produzir geodados de melhor qualidade.
653 $aConditional planning
653 $aGeodata quality
653 $aGeographic Web Services
653 $aGeoprocessamento
653 $aGeoprocessing
653 $aPlanejamento condicional
653 $aQualidade de geodado
653 $aWeb Services geográfcos
700 1 $aMONTEIRO, A. M. V.
700 1 $aSANTOS, R.
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Embrapa Agricultura Digital (CNPTIA) |
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