|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Instrumentação. |
Data corrente: |
06/09/2023 |
Data da última atualização: |
13/09/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ZANETTE, R. de S. S.; FAYER, L.; VASCONCELLOS, R.; OLIVEIRA, L. F. C. de; MARANDUBA, C. M. da C.; ALVARENGA, E. L. F. C. de; MARTINS, M. A.; BRANDAO, H. de M.; MUNK, M. |
Afiliação: |
RAFAELLA DE SOUZA SALOMÃO ZANETTE, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; LEONARA FAYER, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; REBECCA VASCONCELLOS, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; LUIZ FERNANDO CAPPA DE OLIVEIRA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; CARLOS MAGNO DA COSTA MARANDUBA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; ERIKA LORENA FONSECA COSTA DE ALVARENGA, UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL-REY; MARIA ALICE MARTINS, CNPDIA; HUMBERTO DE MELLO BRANDAO, CNPGL; MICHELE MUNK, UNIVERSIDADE FEDERAL DE JUIZ DE FORA. |
Título: |
Cytocompatible and osteoinductive cotton cellulose nanofiber/chitosan nanobiocomposite scaffold for bone tissue engineering. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Biomedical Materials, v. 18, 055016, 2023. |
DOI: |
http://doi.org/10.1088/1748-605X/aceac8 |
Idioma: |
Inglês |
Conteúdo: |
Natural polymeric nanobiocomposites hold promise in repairing damaged bone tissue in tissue engineering. These materials create an extracellular matrix (ECM)-like microenvironment that induces stem cell differentiation. In this study, we investigated a new cytocompatible nanobiocomposite made from cotton cellulose nanofibers (CNFs) combined with chitosan polymer to induce osteogenic stem cell differentiation. First, we characterized the chemical composition, nanotopography, swelling properties, and mechanical properties of the cotton CNF/chitosan nanobiocomposite scaffold. Then, we examined the biological characteristics of the nanocomposites to evaluate their cytocompatibility and osteogenic differentiation potential using human mesenchymal stem cells derived from exfoliated deciduous teeth. The results showed that the nanobiocomposite exhibited favorable cytocompatibility and promoted osteogenic differentiation of cells without the need for chemical inducers, as demonstrated by the increase in alkaline phosphatase activity and ECM mineralization. Therefore, the cotton CNF/chitosan nanobiocomposite scaffold holds great promise for bone tissue engineering applications. |
Palavras-Chave: |
Biomedical nanomaterials; Human mesenchymal; Nanofibra; Tecido ósseo. |
Thesagro: |
Algodão; Célula; Celulose. |
Thesaurus Nal: |
Cell differentiation; Nanomaterials; Stem cells. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02235naa a2200349 a 4500 001 2156507 005 2023-09-13 008 2023 bl uuuu u00u1 u #d 024 7 $ahttp://doi.org/10.1088/1748-605X/aceac8$2DOI 100 1 $aZANETTE, R. de S. S. 245 $aCytocompatible and osteoinductive cotton cellulose nanofiber/chitosan nanobiocomposite scaffold for bone tissue engineering.$h[electronic resource] 260 $c2023 520 $aNatural polymeric nanobiocomposites hold promise in repairing damaged bone tissue in tissue engineering. These materials create an extracellular matrix (ECM)-like microenvironment that induces stem cell differentiation. In this study, we investigated a new cytocompatible nanobiocomposite made from cotton cellulose nanofibers (CNFs) combined with chitosan polymer to induce osteogenic stem cell differentiation. First, we characterized the chemical composition, nanotopography, swelling properties, and mechanical properties of the cotton CNF/chitosan nanobiocomposite scaffold. Then, we examined the biological characteristics of the nanocomposites to evaluate their cytocompatibility and osteogenic differentiation potential using human mesenchymal stem cells derived from exfoliated deciduous teeth. The results showed that the nanobiocomposite exhibited favorable cytocompatibility and promoted osteogenic differentiation of cells without the need for chemical inducers, as demonstrated by the increase in alkaline phosphatase activity and ECM mineralization. Therefore, the cotton CNF/chitosan nanobiocomposite scaffold holds great promise for bone tissue engineering applications. 650 $aCell differentiation 650 $aNanomaterials 650 $aStem cells 650 $aAlgodão 650 $aCélula 650 $aCelulose 653 $aBiomedical nanomaterials 653 $aHuman mesenchymal 653 $aNanofibra 653 $aTecido ósseo 700 1 $aFAYER, L. 700 1 $aVASCONCELLOS, R. 700 1 $aOLIVEIRA, L. F. C. de 700 1 $aMARANDUBA, C. M. da C. 700 1 $aALVARENGA, E. L. F. C. de 700 1 $aMARTINS, M. A. 700 1 $aBRANDAO, H. de M. 700 1 $aMUNK, M. 773 $tBiomedical Materials$gv. 18, 055016, 2023.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Instrumentação (CNPDIA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
19/11/2018 |
Data da última atualização: |
22/02/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
LAMMEL, D. R.; BARTH, G.; OVASKAINEN, O.; CRUZ, L. M.; ZANATTA, J. A.; RYO, M.; SOUZA, E. M. de; PEDROSA, F. O. |
Afiliação: |
Daniel R. Lammel, UFPR; Gabriel Barth, ABC Research Foundation; Otso Ovaskainen, University of Helsinki; Leonardo M. Cruz, UFPR; JOSILEIA ACORDI ZANATTA, CNPF; Masahiro Ryo, 3Freie Universität Berlin and Berlin-Brandenburg Institute of Advanced Biodiversity Research (BBIB); Emanuel M. de Souza, UFPR; Fábio O. Pedrosa, UFPR. |
Título: |
Direct and indirect effects of a pH gradient bring insights into the mechanisms driving prokaryotic community structures. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Microbiome, v. 6, article 106, June 2018. 13 p. |
DOI: |
10.1186/s40168-018-0482-8 |
Idioma: |
Inglês |
Conteúdo: |
Background: pH is frequently reported as the main driver for prokaryotic community structure in soils. However, pH changes are also linked to ?spillover effects? on other chemical parameters (e.g., availability of Al, Fe, Mn, Zn, and Cu) and plant growth, but these indirect effects on the microbial communities are rarely investigated. Usually, pH also co-varies with some confounding factors, such as land use, soil management (e.g., tillage and chemical inputs), plant cover, and/or edapho-climatic conditions. So, a more comprehensive analysis of the direct and indirect effects of pH brings a better understanding of the mechanisms driving prokaryotic (archaeal and bacterial) community structures. Results: We evaluated an agricultural soil pH gradient (from 4 to 6, the typical range for tropical farms), in a liming gradient with confounding factors minimized, investigating relationships between prokaryotic communities (16S rRNA) and physical?chemical parameters (indirect effects). Correlations, hierarchical modeling of species communities (HMSC), and random forest (RF) modeling indicated that both direct and indirect effects of the pH gradient affected the prokaryotic communities. Some OTUs were more affected by the pH changes (e.g., some Actinobacteria), while others were more affected by the indirect pH effects (e.g., some Proteobacteria). HMSC detected a phylogenetic signal related to the effects. Both HMSC and RF indicated that the main indirect effect was the pH changes on the availability of some elements (e.g., Al, Fe, and Cu), and secondarily, effects on plant growth and nutrient cycling also affected the OTUs. Additionally, we found that some of the OTUs that responded to pH also correlated with CO2, CH4, and N2O greenhouse gas fluxes. Conclusions: Our results indicate that there are two distinct pH-related mechanisms driving prokaryotic community structures, the direct effect and ?spillover effects? of pH (indirect effects). Moreover, the indirect effects are highly relevant for some OTUs and consequently for the community structure; therefore, it is a mechanism that should be further investigated in microbial ecology. MenosBackground: pH is frequently reported as the main driver for prokaryotic community structure in soils. However, pH changes are also linked to ?spillover effects? on other chemical parameters (e.g., availability of Al, Fe, Mn, Zn, and Cu) and plant growth, but these indirect effects on the microbial communities are rarely investigated. Usually, pH also co-varies with some confounding factors, such as land use, soil management (e.g., tillage and chemical inputs), plant cover, and/or edapho-climatic conditions. So, a more comprehensive analysis of the direct and indirect effects of pH brings a better understanding of the mechanisms driving prokaryotic (archaeal and bacterial) community structures. Results: We evaluated an agricultural soil pH gradient (from 4 to 6, the typical range for tropical farms), in a liming gradient with confounding factors minimized, investigating relationships between prokaryotic communities (16S rRNA) and physical?chemical parameters (indirect effects). Correlations, hierarchical modeling of species communities (HMSC), and random forest (RF) modeling indicated that both direct and indirect effects of the pH gradient affected the prokaryotic communities. Some OTUs were more affected by the pH changes (e.g., some Actinobacteria), while others were more affected by the indirect pH effects (e.g., some Proteobacteria). HMSC detected a phylogenetic signal related to the effects. Both HMSC and RF indicated that the main indirect effect was the pH changes on... Mostrar Tudo |
Palavras-Chave: |
16S rRNA; Ecologia microbiana; Illumina sequencing; Solo sub tropical; Sub-tropical soil. |
Thesagro: |
Bactéria; Ph; Química do Solo. |
Thesaurus NAL: |
Archaea; Microbial ecology; Soil chemistry. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/186279/1/2018-Josi-Microbiome-Direct.pdf
|
Marc: |
LEADER 03160naa a2200349 a 4500 001 2099575 005 2019-02-22 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1186/s40168-018-0482-8$2DOI 100 1 $aLAMMEL, D. R. 245 $aDirect and indirect effects of a pH gradient bring insights into the mechanisms driving prokaryotic community structures.$h[electronic resource] 260 $c2018 520 $aBackground: pH is frequently reported as the main driver for prokaryotic community structure in soils. However, pH changes are also linked to ?spillover effects? on other chemical parameters (e.g., availability of Al, Fe, Mn, Zn, and Cu) and plant growth, but these indirect effects on the microbial communities are rarely investigated. Usually, pH also co-varies with some confounding factors, such as land use, soil management (e.g., tillage and chemical inputs), plant cover, and/or edapho-climatic conditions. So, a more comprehensive analysis of the direct and indirect effects of pH brings a better understanding of the mechanisms driving prokaryotic (archaeal and bacterial) community structures. Results: We evaluated an agricultural soil pH gradient (from 4 to 6, the typical range for tropical farms), in a liming gradient with confounding factors minimized, investigating relationships between prokaryotic communities (16S rRNA) and physical?chemical parameters (indirect effects). Correlations, hierarchical modeling of species communities (HMSC), and random forest (RF) modeling indicated that both direct and indirect effects of the pH gradient affected the prokaryotic communities. Some OTUs were more affected by the pH changes (e.g., some Actinobacteria), while others were more affected by the indirect pH effects (e.g., some Proteobacteria). HMSC detected a phylogenetic signal related to the effects. Both HMSC and RF indicated that the main indirect effect was the pH changes on the availability of some elements (e.g., Al, Fe, and Cu), and secondarily, effects on plant growth and nutrient cycling also affected the OTUs. Additionally, we found that some of the OTUs that responded to pH also correlated with CO2, CH4, and N2O greenhouse gas fluxes. Conclusions: Our results indicate that there are two distinct pH-related mechanisms driving prokaryotic community structures, the direct effect and ?spillover effects? of pH (indirect effects). Moreover, the indirect effects are highly relevant for some OTUs and consequently for the community structure; therefore, it is a mechanism that should be further investigated in microbial ecology. 650 $aArchaea 650 $aMicrobial ecology 650 $aSoil chemistry 650 $aBactéria 650 $aPh 650 $aQuímica do Solo 653 $a16S rRNA 653 $aEcologia microbiana 653 $aIllumina sequencing 653 $aSolo sub tropical 653 $aSub-tropical soil 700 1 $aBARTH, G. 700 1 $aOVASKAINEN, O. 700 1 $aCRUZ, L. M. 700 1 $aZANATTA, J. A. 700 1 $aRYO, M. 700 1 $aSOUZA, E. M. de 700 1 $aPEDROSA, F. O. 773 $tMicrobiome$gv. 6, article 106, June 2018. 13 p.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Florestas (CNPF) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|