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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
11/10/1995 |
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Data da última atualização: |
11/10/1995 |
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Autoria: |
INDIRA, P.; SINHA, S. K. |
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Afiliação: |
Central Tuber Crops Research Institute, Trivandrum. |
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Título: |
Colorimetric method for determination of HCN in tubers and leaves of cassava (Manihot esculenta crantz. |
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Ano de publicação: |
1969 |
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Fonte/Imprenta: |
Indian Journal Agricultural Science, v.39, p.1021-1023. 1969. |
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Idioma: |
Inglês |
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Conteúdo: |
Accurate methods for the extraction and determination of cyanogenic glucosides have been developed recently by Butler and Conn (1964) and Butler (1965). These methods make use of purified linamarase as a reagentfor the release of HCN. Though these methods are sensitive and accurate, they cannot be used for the determination of cyanoglucosides like HCN in a large number of samples in a routine experiment. In sorghum endogenous enzyme was used by Akazawa et al. (1960) for the release ofHCN from dhurrin. The released HCN was trapped in sodium hydroxide and determined by the method of aldridge (1944). A quick qualitaive method with sodiuin picrate has also been described. Attempts were made to make this method quantitative (Gilchrist et al., 1967). |
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Palavras-Chave: |
Enzimas; Linamorose. |
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Thesagro: |
Cianeto. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01243naa a2200169 a 4500
001 1647403
005 1995-10-11
008 1969 bl --- 0-- u #d
100 1 $aINDIRA, P.
245 $aColorimetric method for determination of HCN in tubers and leaves of cassava (Manihot esculenta crantz.
260 $c1969
520 $aAccurate methods for the extraction and determination of cyanogenic glucosides have been developed recently by Butler and Conn (1964) and Butler (1965). These methods make use of purified linamarase as a reagentfor the release of HCN. Though these methods are sensitive and accurate, they cannot be used for the determination of cyanoglucosides like HCN in a large number of samples in a routine experiment. In sorghum endogenous enzyme was used by Akazawa et al. (1960) for the release ofHCN from dhurrin. The released HCN was trapped in sodium hydroxide and determined by the method of aldridge (1944). A quick qualitaive method with sodiuin picrate has also been described. Attempts were made to make this method quantitative (Gilchrist et al., 1967).
650 $aCianeto
653 $aEnzimas
653 $aLinamorose
700 1 $aSINHA, S. K.
773 $tIndian Journal Agricultural Science$gv.39, p.1021-1023. 1969.
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