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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
18/12/2019 |
Data da última atualização: |
06/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ARAÚJO, R. S.; GARCIA, G. M.; VILELA, J. M. C.; ANDRADE, M. S.; OLIVEIRA, L. A. M.; KANO, E. K.; LANGE, C. C.; BRITO, M. A. V. P. E; BRANDAO, H. de M.; MOSQUEIRA, V. C. F. |
Afiliação: |
RAQUEL SILVA ARAÚJO; GIANI MARTINS GARCIA; JOSÉ MARIO CARNEIRO VILELA; MARGARETH SPANGLER ANDRADE; LASER ANTÔNIO MACHADO OLIVEIRA; EUNICE KAZUE KANO; CARLA CHRISTINE LANGE, CNPGL; MARIA APARECIDA VASCONCELOS PAIVA E BRITO; HUMBERTO DE MELLO BRANDAO, CNPGL; VANESSA CARLA FURTADO MOSQUEIRA. |
Título: |
Cloxacillin benzathine-loaded polymeric nanocapsules: Physicochemical characterization, cell uptake, and intramammary antimicrobial effect. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Materials Science & Engineering C, v. 104, 110006, 2019. |
DOI: |
https://doi.org/10.1016/j.msec.2019.110006 |
Idioma: |
Inglês |
Conteúdo: |
The present work shows the development and evaluation of the veterinary antibiotic cloxacillin benzathine(CLOXB) loaded into poly-ε-caprolactone (PCL) nanocapsules (NC), as a potential new treatment strategy tomanage bovine intramammary infections, such as mastitis.Staphylococcus aureus-induced mastitis is often arecurrent disease due to the persistence of bacteria within infected cells. CLOXB-PCL NC were prepared byinterfacial deposition of preformed biodegradable polymer followed by solvent displacement method. The meandiameter of NC varied from 241 to 428 nm and from 326 to 375 nm, when determined by dynamic light scat-tering and by atomic force microscopy, respectively. The zeta potential of NC was negative and varied from−28to−51 mV.In vitrorelease studies from the NC were performed in two media undersinkconditions: PBS with1% polyethylene glycol or milk. A reversed-phase HPLC method was developed to determine the NC entrapmentefficiency and kinetics of CLOXB release from the NC. Free CLOXB dissolution occurred very fast in both media,while drug release from the NC was slower and incomplete (below 50%) after 9 h. CLOXB release kinetics frompolymeric NC wasfitted with the Korsmeyer-Peppas model indicating that CLOXB release is governed by dif-fusion following Fick's law. Thefluorescence confocal microscopy images of macrophage-like J774A.1 cellsreveal NC uptake and internalizationin vitro. In addition, antimicrobial effect of the intramammary adminis-tration of CLOXB-PCL NC in cows with mastitis resulted in no clinical signs of toxicity and allowed completepathogen elimination after treatment. Thein vivoresults obtained in this work suggest that CLOXB-PCL NC couldbe a promising formulation for the treatment of intramammary infections in cattle, considering their physico-chemical properties, release profiles and effects on bovine mastitis control. MenosThe present work shows the development and evaluation of the veterinary antibiotic cloxacillin benzathine(CLOXB) loaded into poly-ε-caprolactone (PCL) nanocapsules (NC), as a potential new treatment strategy tomanage bovine intramammary infections, such as mastitis.Staphylococcus aureus-induced mastitis is often arecurrent disease due to the persistence of bacteria within infected cells. CLOXB-PCL NC were prepared byinterfacial deposition of preformed biodegradable polymer followed by solvent displacement method. The meandiameter of NC varied from 241 to 428 nm and from 326 to 375 nm, when determined by dynamic light scat-tering and by atomic force microscopy, respectively. The zeta potential of NC was negative and varied from−28to−51 mV.In vitrorelease studies from the NC were performed in two media undersinkconditions: PBS with1% polyethylene glycol or milk. A reversed-phase HPLC method was developed to determine the NC entrapmentefficiency and kinetics of CLOXB release from the NC. Free CLOXB dissolution occurred very fast in both media,while drug release from the NC was slower and incomplete (below 50%) after 9 h. CLOXB release kinetics frompolymeric NC wasfitted with the Korsmeyer-Peppas model indicating that CLOXB release is governed by dif-fusion following Fick's law. Thefluorescence confocal microscopy images of macrophage-like J774A.1 cellsreveal NC uptake and internalizationin vitro. In addition, antimicrobial effect of the intramammary adminis-tra... Mostrar Tudo |
Palavras-Chave: |
Cell uptake; Cloxacillin benzathine; Intramammary; Release kinetics. |
Thesaurus Nal: |
Mastitis; Nanocapsules. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02882naa a2200313 a 4500 001 2117206 005 2024-02-06 008 2019 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.msec.2019.110006$2DOI 100 1 $aARAÚJO, R. S. 245 $aCloxacillin benzathine-loaded polymeric nanocapsules$bPhysicochemical characterization, cell uptake, and intramammary antimicrobial effect.$h[electronic resource] 260 $c2019 520 $aThe present work shows the development and evaluation of the veterinary antibiotic cloxacillin benzathine(CLOXB) loaded into poly-ε-caprolactone (PCL) nanocapsules (NC), as a potential new treatment strategy tomanage bovine intramammary infections, such as mastitis.Staphylococcus aureus-induced mastitis is often arecurrent disease due to the persistence of bacteria within infected cells. CLOXB-PCL NC were prepared byinterfacial deposition of preformed biodegradable polymer followed by solvent displacement method. The meandiameter of NC varied from 241 to 428 nm and from 326 to 375 nm, when determined by dynamic light scat-tering and by atomic force microscopy, respectively. The zeta potential of NC was negative and varied from−28to−51 mV.In vitrorelease studies from the NC were performed in two media undersinkconditions: PBS with1% polyethylene glycol or milk. A reversed-phase HPLC method was developed to determine the NC entrapmentefficiency and kinetics of CLOXB release from the NC. Free CLOXB dissolution occurred very fast in both media,while drug release from the NC was slower and incomplete (below 50%) after 9 h. CLOXB release kinetics frompolymeric NC wasfitted with the Korsmeyer-Peppas model indicating that CLOXB release is governed by dif-fusion following Fick's law. Thefluorescence confocal microscopy images of macrophage-like J774A.1 cellsreveal NC uptake and internalizationin vitro. In addition, antimicrobial effect of the intramammary adminis-tration of CLOXB-PCL NC in cows with mastitis resulted in no clinical signs of toxicity and allowed completepathogen elimination after treatment. Thein vivoresults obtained in this work suggest that CLOXB-PCL NC couldbe a promising formulation for the treatment of intramammary infections in cattle, considering their physico-chemical properties, release profiles and effects on bovine mastitis control. 650 $aMastitis 650 $aNanocapsules 653 $aCell uptake 653 $aCloxacillin benzathine 653 $aIntramammary 653 $aRelease kinetics 700 1 $aGARCIA, G. M. 700 1 $aVILELA, J. M. C. 700 1 $aANDRADE, M. S. 700 1 $aOLIVEIRA, L. A. M. 700 1 $aKANO, E. K. 700 1 $aLANGE, C. C. 700 1 $aBRITO, M. A. V. P. E 700 1 $aBRANDAO, H. de M. 700 1 $aMOSQUEIRA, V. C. F. 773 $tMaterials Science & Engineering C$gv. 104, 110006, 2019.
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Embrapa Gado de Leite (CNPGL) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
23/02/2015 |
Data da última atualização: |
15/03/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
PAGAN, C.; COYNE, D.; KARIUKI, G.; CARNEIRO, R. M. D. G.; WILLIAMSON, V. M. |
Afiliação: |
C. PAGAN, UNIV. OF CALIFORNIA, USA; D. COYNE, INTERNATIONAL INSTITUTE OF TROPICAL AGRICULTURE (IITA) EAST AFRICA , KENYA; G. KARIUKI, KENYATTA UNIVERSITY, KENYA; REGINA MARIA DECHECHI G CARNEIRO, CENARGEN; V. M. WILLIAMSON, UNIV. OF CALIFORNIA, USA. |
Título: |
Mitochondrial haplotypes for identification of tropical root-knot nematode species and Lineages. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Journal of Nematology, v. 46, n. 2, p. 216, June 2014. |
Idioma: |
Inglês |
Notas: |
Edição dos Proceedings do 6th International Congress of Nematology, Cape Town, South Africa, May 2014. |
Palavras-Chave: |
RKN. |
Thesagro: |
Meloidogyne spp. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00660nam a2200181 a 4500 001 2009409 005 2023-03-15 008 2014 bl uuuu u00u1 u #d 100 1 $aPAGAN, C. 245 $aMitochondrial haplotypes for identification of tropical root-knot nematode species and Lineages.$h[electronic resource] 260 $aJournal of Nematology, v. 46, n. 2, p. 216, June 2014.$c2014 500 $aEdição dos Proceedings do 6th International Congress of Nematology, Cape Town, South Africa, May 2014. 650 $aMeloidogyne spp 653 $aRKN 700 1 $aCOYNE, D. 700 1 $aKARIUKI, G. 700 1 $aCARNEIRO, R. M. D. G. 700 1 $aWILLIAMSON, V. M.
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