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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
18/06/1997 |
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Data da última atualização: |
08/08/2019 |
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Autoria: |
GONZALEZ-BENITO, M. E.; CARVALHO, J. M. F.-C.; PEREZ, C. |
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Afiliação: |
MARIA ELENA GONZÁLEZ-BENITO, Universidad Politécnica de Madrid/Dep. de Biologia Vegetal; JULITA MARIA FROTA-CHAGAS CARVALHO, Universidad Politécnica de Madrid/Dep. de Biologia Vegetal; CÉSAR PÉREZ, Universidad Politécnica de Madrid/Dep. de Biologia Vegetal. |
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Título: |
Somatic embryogenesis of an early cotton cultivar. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 32, n. 5, p. 485-488, maio 1997. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Embriogênese somática na cultivar precoce do algodão. |
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Conteúdo: |
Somatic embryogenesis in cotton lias been previously reported in a limited number of genotypes, predominantly Coker cultivars. Before tissue culture techniques are widely applied to cotton improvement prograins, regeneration must be possible for a broad range of genotypes. The Mm of the present work was to develop a method to obtain somatic embryoids of an early cotton cultivar (Gossypium hirsutum L. race lar ifolium Hutch cv. CNPA Precoce 2) and their subsequent development. Calius induction was attempted with cotyledon and hypocotyl explants. These explants were cultured on MS medium supplemented with tive concentrations of 2,4-dichlorophenoxyacetic acid (2,4-13) and N6-(2-isopentenyi)-adenine (2iP) either alone or in combination. Based on cailus appearance (light brown and granular), four different growth regulator combinations were selected for further cailus development. Callus was subcultured on 2.45 uM 2iP and subsequently transferred to 0.45 and 22.50 tiM 2,4-D. Somatic embryos of different sizes and shapes subsequently appeared on MS medium supplemented with 2 g L» glutamine and no growth regulators. Plantlets were developed from those embryoids. Plant regeneration through somatic embryogenesis is achieved for fite first time in the early cultivar CNPA Precoce 2. |
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Palavras-Chave: |
Calos; Embrioes somaticos; Plant regeneration; Regeneracao da planta. |
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Thesaurus Nal: |
callus; somatic embryos. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/14199/1/pab97_05_maio.pdf
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Marc: |
LEADER 02006naa a2200229 a 4500
001 1098459
005 2019-08-08
008 1997 bl uuuu u00u1 u #d
100 1 $aGONZALEZ-BENITO, M. E.
245 $aSomatic embryogenesis of an early cotton cultivar.
260 $c1997
500 $aTítulo em português: Embriogênese somática na cultivar precoce do algodão.
520 $aSomatic embryogenesis in cotton lias been previously reported in a limited number of genotypes, predominantly Coker cultivars. Before tissue culture techniques are widely applied to cotton improvement prograins, regeneration must be possible for a broad range of genotypes. The Mm of the present work was to develop a method to obtain somatic embryoids of an early cotton cultivar (Gossypium hirsutum L. race lar ifolium Hutch cv. CNPA Precoce 2) and their subsequent development. Calius induction was attempted with cotyledon and hypocotyl explants. These explants were cultured on MS medium supplemented with tive concentrations of 2,4-dichlorophenoxyacetic acid (2,4-13) and N6-(2-isopentenyi)-adenine (2iP) either alone or in combination. Based on cailus appearance (light brown and granular), four different growth regulator combinations were selected for further cailus development. Callus was subcultured on 2.45 uM 2iP and subsequently transferred to 0.45 and 22.50 tiM 2,4-D. Somatic embryos of different sizes and shapes subsequently appeared on MS medium supplemented with 2 g L» glutamine and no growth regulators. Plantlets were developed from those embryoids. Plant regeneration through somatic embryogenesis is achieved for fite first time in the early cultivar CNPA Precoce 2.
650 $acallus
650 $asomatic embryos
653 $aCalos
653 $aEmbrioes somaticos
653 $aPlant regeneration
653 $aRegeneracao da planta
700 1 $aCARVALHO, J. M. F.-C.
700 1 $aPEREZ, C.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 32, n. 5, p. 485-488, maio 1997.
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Embrapa Unidades Centrais (AI-SEDE) |
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