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Registro Completo |
Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
08/03/2010 |
Data da última atualização: |
13/08/2019 |
Tipo da produção científica: |
Artigo de Divulgação na Mídia |
Autoria: |
PEREIRA, G. E. |
Afiliação: |
GIULIANO ELIAS PEREIRA, CNPUV. |
Título: |
Vinhos tropicais do Brasil. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Informativo ABE, Bento Gonçalves, n. 67, p. 6, 2010. |
Idioma: |
Português |
Conteúdo: |
o cultivo da videira destinado à produção de uvas para a elaboração de vinhos finos tem apresentado crescimento substancial nos últimos anos, em diversos países do mundo, inclusive no Brasil. Este crescimento representa não somente o aumento das áreas existentes, como também no aparecimento de novos pólos vitivinícolas, que exigem a utilização de tecnologias aplicadas ao sistema de produção das uvas, ao processamento e à elaboração dos vinhos. |
Palavras-Chave: |
Brasil; Vale do Submédio São Francisco; Vinho tropical. |
Thesagro: |
Enologia; Produção; Uva; Variedade; Vinho. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/200651/1/12008-2010-p.6.pdf
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Marc: |
LEADER 00995nam a2200205 a 4500 001 1660129 005 2019-08-13 008 2010 bl uuuu u00u1 u #d 100 1 $aPEREIRA, G. E. 245 $aVinhos tropicais do Brasil.$h[electronic resource] 260 $aInformativo ABE, Bento Gonçalves, n. 67, p. 6$c2010 520 $ao cultivo da videira destinado à produção de uvas para a elaboração de vinhos finos tem apresentado crescimento substancial nos últimos anos, em diversos países do mundo, inclusive no Brasil. Este crescimento representa não somente o aumento das áreas existentes, como também no aparecimento de novos pólos vitivinícolas, que exigem a utilização de tecnologias aplicadas ao sistema de produção das uvas, ao processamento e à elaboração dos vinhos. 650 $aEnologia 650 $aProdução 650 $aUva 650 $aVariedade 650 $aVinho 653 $aBrasil 653 $aVale do Submédio São Francisco 653 $aVinho tropical
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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Registro Completo
Biblioteca(s): |
Embrapa Agricultura Digital. |
Data corrente: |
07/02/2019 |
Data da última atualização: |
07/02/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
GUIMARÃES, M. Z. P.; DE VECCHI, R.; VITÓRIA, G.; SOCHACKI, J. K.; PAULSEN, B. S.; LIMA, I.; SILVA, F. R. da; COSTA, R. F. M. da; CASTRO, N. G.; BRETON, L.; REHEN, S. K. |
Afiliação: |
MARÍLIA Z. P. GUIMARÃES, D’Or Institute for Research and Education, UFRJ; RODRIGO DE VECCHI, L’Oréal Research & Innovation, Unicamp; GABRIELA VITÓRIA, L’Oréal Research & Innovation; JAROSLAW K. SOCHACKI, L’Oréal Research & Innovation; BRUNA S. PAULSEN, L’Oréal Research & Innovation; IGOR LIMA, L’Oréal Research & Innovation; FELIPE RODRIGUES DA SILVA, CNPTIA, Unicamp; RODRIGO F. M. DA COSTA, L’Oréal Research & Innovation; NEWTON G. CASTRO, UFRJ; LIONEL BRETON, L’Oréal Research & Innovation; STEVENS K. REHEN, D’Or Institute for Research and Education, UFRJ. |
Título: |
Generation of iPSC-derived human peripheral sensory neurons releasing substance P elicited by TRPV1 agonists. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Frontiers in Molecular Neuroscience, v. 11, p. 1-17, Aug. 2018. |
DOI: |
10.3389/fnmol.2018.00277 |
Idioma: |
Inglês |
Notas: |
Article 277. |
Conteúdo: |
Neural crest stem cells (NCPCs) have been shown to differentiate into various cell types and tissues during embryonic development, including sensory neurons. The few studies addressing the generation of NCPCs and peripheral sensory neurons (PSNs) from human induced pluripotent stem cells (hiPSCs), generated sensory cells without displaying robust activity. Here, we describe an efficient strategy for hiPSCs differentiation into NCPCs and functional PSNs using chemically defined media and factors to achieve efficient differentiation, confirmed by the expression of specific markers. After 10 days hiPSCs differentiated into NCPCs, cells were then maintained in neural induction medium containing defined growth factors for PSNs differentiation, followed by 10 days in neonatal human epidermal keratinocytes- (HEKn-) conditioned medium (CM). We observed a further increase in PSN markers expression and neurites length after CM treatment. The resulting neurons elicited action potentials after current injection and released substance P (SP) in response to nociceptive agents such as anandamide and resiniferatoxin. Anandamide induced substance P release via activation of TRPV1 and not CB1. Transcriptomic analysis of the PSNs revealed the main dorsal root ganglia neuronal markers and a transcriptional profile compatible with C fiber-low threshold mechanoreceptors. TRPV1 was detected by immunofluorescence and RNA-Seq in multiple experiments. In conclusion, the developed strategy generated PSNs useful for drug screening that could be applied to patient-derived hiPSCs, consisting in a powerful tool to model human diseases in vitro. MenosNeural crest stem cells (NCPCs) have been shown to differentiate into various cell types and tissues during embryonic development, including sensory neurons. The few studies addressing the generation of NCPCs and peripheral sensory neurons (PSNs) from human induced pluripotent stem cells (hiPSCs), generated sensory cells without displaying robust activity. Here, we describe an efficient strategy for hiPSCs differentiation into NCPCs and functional PSNs using chemically defined media and factors to achieve efficient differentiation, confirmed by the expression of specific markers. After 10 days hiPSCs differentiated into NCPCs, cells were then maintained in neural induction medium containing defined growth factors for PSNs differentiation, followed by 10 days in neonatal human epidermal keratinocytes- (HEKn-) conditioned medium (CM). We observed a further increase in PSN markers expression and neurites length after CM treatment. The resulting neurons elicited action potentials after current injection and released substance P (SP) in response to nociceptive agents such as anandamide and resiniferatoxin. Anandamide induced substance P release via activation of TRPV1 and not CB1. Transcriptomic analysis of the PSNs revealed the main dorsal root ganglia neuronal markers and a transcriptional profile compatible with C fiber-low threshold mechanoreceptors. TRPV1 was detected by immunofluorescence and RNA-Seq in multiple experiments. In conclusion, the developed strategy generated P... Mostrar Tudo |
Palavras-Chave: |
Human induced pluripotent stem cells; Neural crest stem cells; Neurônios sensoriais; Pré-clínico; Substância P; TRPV1. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/192302/1/AP-Generation-iPSC-Felipe.pdf
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Marc: |
LEADER 02655naa a2200337 a 4500 001 2105696 005 2019-02-07 008 2018 bl uuuu u00u1 u #d 024 7 $a10.3389/fnmol.2018.00277$2DOI 100 1 $aGUIMARÃES, M. Z. P. 245 $aGeneration of iPSC-derived human peripheral sensory neurons releasing substance P elicited by TRPV1 agonists.$h[electronic resource] 260 $c2018 500 $aArticle 277. 520 $aNeural crest stem cells (NCPCs) have been shown to differentiate into various cell types and tissues during embryonic development, including sensory neurons. The few studies addressing the generation of NCPCs and peripheral sensory neurons (PSNs) from human induced pluripotent stem cells (hiPSCs), generated sensory cells without displaying robust activity. Here, we describe an efficient strategy for hiPSCs differentiation into NCPCs and functional PSNs using chemically defined media and factors to achieve efficient differentiation, confirmed by the expression of specific markers. After 10 days hiPSCs differentiated into NCPCs, cells were then maintained in neural induction medium containing defined growth factors for PSNs differentiation, followed by 10 days in neonatal human epidermal keratinocytes- (HEKn-) conditioned medium (CM). We observed a further increase in PSN markers expression and neurites length after CM treatment. The resulting neurons elicited action potentials after current injection and released substance P (SP) in response to nociceptive agents such as anandamide and resiniferatoxin. Anandamide induced substance P release via activation of TRPV1 and not CB1. Transcriptomic analysis of the PSNs revealed the main dorsal root ganglia neuronal markers and a transcriptional profile compatible with C fiber-low threshold mechanoreceptors. TRPV1 was detected by immunofluorescence and RNA-Seq in multiple experiments. In conclusion, the developed strategy generated PSNs useful for drug screening that could be applied to patient-derived hiPSCs, consisting in a powerful tool to model human diseases in vitro. 653 $aHuman induced pluripotent stem cells 653 $aNeural crest stem cells 653 $aNeurônios sensoriais 653 $aPré-clínico 653 $aSubstância P 653 $aTRPV1 700 1 $aDE VECCHI, R. 700 1 $aVITÓRIA, G. 700 1 $aSOCHACKI, J. K. 700 1 $aPAULSEN, B. S. 700 1 $aLIMA, I. 700 1 $aSILVA, F. R. da 700 1 $aCOSTA, R. F. M. da 700 1 $aCASTRO, N. G. 700 1 $aBRETON, L. 700 1 $aREHEN, S. K. 773 $tFrontiers in Molecular Neuroscience$gv. 11, p. 1-17, Aug. 2018.
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