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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
29/11/2020 |
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Data da última atualização: |
29/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GALIZA, Y. S. de; ELOY, A. M. X.; PINHEIRO, R. R.; PEIXOTO, R. M.; LIMA, A. M. C.; BARROSO, M. L. da S.; FONSECA, L. M. |
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Afiliação: |
YLANA SANTOS DE GALIZA; ANGELA MARIA XAVIER ELOY, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; RENATO MESQUITA PEIXOTO; ANA MILENA CÉSAR LIMA; MARIA LUANE DA SILVA BARROSO; LUZIANNA MACEDO FONSECA. |
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Título: |
Study of metalloproteinases in the blood of goats experimentally infected with caprine encephalitis arthritis virus. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 41, n. 6, p. 3165-3176, 2020. Suplemento 2. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Caprine arthritis encephalitis is a lentiviral disease that leads to considerable losses in goat farming. In the acute phase of viral infection, though antiviral antibodies are produced by the host?s immune system, they are not sufficient to be detected by serological tests. Acute infections begin with an incubation period, during which the viral genome replicates and host innate responses are initiated. Matrix metalloproteinases (MMPs) are enzymes that play an important role in the physiological and pathological processes of tissue remodeling. The present study aimed to evaluate the expression of MMPs and their activity in the blood serum of male goats experimentally infected with caprine arthritis encephalitis virus (CAEV). Five dairy male goats, aged 3-4 years, were intravenously inoculated with CAEV Cork strain (titer: 105-6 TCID50/mL) after being tested negative for CAEV thrice at consecutive intervals of 30 days using western blot analysis and nested-PCR. The study included three stages: S1 or pre-infection stage; S2 or seroconversion stage, corresponding to the occurrence of first seroconversion; and S3 or post-seroconversion stage, corresponding to 23 weeks after seroconversion. Zymography was performed for the samples using gelatin zymography gels (12.5%), which were subjected to electrophoresis at 170V, 1A, and 300W for 50-70 min. The density of MMP-2 was found to be lower at S1 (1456.20 pixels) than that at S2 and S3 (1943.80 and 2104.40 pixels, respectively) (P < 0.05); and the density of MMP-9 was found to be lower at S3 (133.60 pixels) than that at S1 and S2 (359.60 and 370.60 pixels, respectively). The density of proMMP-2 was low at S1 and S3 (130.45 and 145.20 pixels, respectively). On the other hand, the density of proMMP-9 was statistically different between S1 and S3 (89.22 vs. 415.60 pixels). Both proMMP-2 and proMMP-9 were absent at S2. Thus, MMP-2 and MMP-9 exhibited opposite behaviors depending on the stage of infection. As the greatest activity of MMP-2 was detected at stage S3, we suggest that MMP-2 can be used as a biomarker for complementary diagnosis of acute CAEV infection. In addition, the presence of proMMP-13 can be used to indicate active viral infection. [Estudo de metaloproteinases no sangue de caprinos experimentalmente infectados pelo vírus da artrite encefalite caprina]. Resumo: A encefalite por artrite caprina é uma doença lentiviral que leva a perdas consideráveis na criação de caprinos. Na fase aguda da infecção viral, embora os anticorpos antivirais sejam produzidos pelo sistema imunológico do hospedeiro, eles não são suficientes para serem detectados por testes sorológicos. As infecções agudas começam com um período de incubação, durante o qual o genoma viral se replica e as respostas inatas do hospedeiro são iniciadas. As metaloproteinases da matriz (MMPs) são enzimas que desempenham um papel importante nos processos fisiológicos e patológicos da remodelação tecidual. O presente estudo teve como objetivo avaliar a expressão de MMPs e sua atividade no soro sanguíneo de reprodutores caprinos infectados experimentalmente pelo vírus da encefalite por artrite caprina (CAEV). Cinco machos caprinos, com idades entre 3-4 anos, foram inoculadas por via intravenosa com a cepa de CAEV Cork (título: 105-6 TCID50/mL) após serem testados negativamente para CAEV três vezes em intervalos consecutivos de 30 dias usando a análise de western blot e nested-PCR. O estudo incluiu três etapas: estágio S1 ou pré-infecção; S2 ou estágio de soroconversão, correspondente à ocorrência de primeira soroconversão; e S3 ou pós-soroconversão, correspondendo a 23 semanas após a soroconversão. A zimografia foi realizada para as amostras utilizando gel de gelatina (12,5%), que foram submetidos à eletroforese em 170V, 1A e 300W por 50-70 min. Verificou-se que a densidade de MMP-2 era menor em S1 (1456,20 pixels) do que em S2 e S3 (1943,80 e 2104,40 pixels, respectivamente) (P < 0,05); e a densidade de MMP-9 foi menor em S3 (133,60 pixels) do que em S1 e S2 (359,60 e 370,60 pixels, respectivamente). A densidade do proMMP-2 era baixa em S1 e S3 (130,45 e 145,20 pixels, respectivamente). Por outro lado, a densidade do proMMP-9 foi estatisticamente diferente entre S1 e S3 (89,22 vs. 415,60 pixels). ProMMP-2 e proMMP-9 estavam ausentes no S2. Assim, MMP-2 e MMP-9 exibiram comportamentos opostos, dependendo do estágio da infecção. Como a maior atividade da MMP-2 foi detectada no estágio S3, sugerimos que a MMP-2 possa ser usada como biomarcador para o diagnóstico complementar de infecção aguda por CAEV. Além disso, a presença de proMMP-13 pode ser usada para indicar infecção viral ativa. MenosAbstract: Caprine arthritis encephalitis is a lentiviral disease that leads to considerable losses in goat farming. In the acute phase of viral infection, though antiviral antibodies are produced by the host?s immune system, they are not sufficient to be detected by serological tests. Acute infections begin with an incubation period, during which the viral genome replicates and host innate responses are initiated. Matrix metalloproteinases (MMPs) are enzymes that play an important role in the physiological and pathological processes of tissue remodeling. The present study aimed to evaluate the expression of MMPs and their activity in the blood serum of male goats experimentally infected with caprine arthritis encephalitis virus (CAEV). Five dairy male goats, aged 3-4 years, were intravenously inoculated with CAEV Cork strain (titer: 105-6 TCID50/mL) after being tested negative for CAEV thrice at consecutive intervals of 30 days using western blot analysis and nested-PCR. The study included three stages: S1 or pre-infection stage; S2 or seroconversion stage, corresponding to the occurrence of first seroconversion; and S3 or post-seroconversion stage, corresponding to 23 weeks after seroconversion. Zymography was performed for the samples using gelatin zymography gels (12.5%), which were subjected to electrophoresis at 170V, 1A, and 300W for 50-70 min. The density of MMP-2 was found to be lower at S1 (1456.20 pixels) than that at S2 and S3 (1943.80 and 2104.40 pixels, respecti... Mostrar Tudo |
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Palavras-Chave: |
CAEV; Caprine arthritis encephalit virus; Encefalite artrite caprina; Experimental infection; Infecção experimental; MMPs; Zimografia; Zymography. |
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Thesagro: |
Caprino; Diagnostico; Doença Animal; Protease; Virose Animal. |
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Thesaurus Nal: |
Brazil; Caprine arthritis encephalitis virus; Caprine arthritis-encephalitis; Disease diagnosis; Goat diseases; Goats; Infectious diseases; Lentivirus; Semiarid zones; Small ruminants. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218348/1/CNPC-2020-Art-41.pdf
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Marc: |
LEADER 06132naa a2200469 a 4500
001 2127176
005 2020-11-29
008 2020 bl uuuu u00u1 u #d
100 1 $aGALIZA, Y. S. de
245 $aStudy of metalloproteinases in the blood of goats experimentally infected with caprine encephalitis arthritis virus.$h[electronic resource]
260 $c2020
520 $aAbstract: Caprine arthritis encephalitis is a lentiviral disease that leads to considerable losses in goat farming. In the acute phase of viral infection, though antiviral antibodies are produced by the host?s immune system, they are not sufficient to be detected by serological tests. Acute infections begin with an incubation period, during which the viral genome replicates and host innate responses are initiated. Matrix metalloproteinases (MMPs) are enzymes that play an important role in the physiological and pathological processes of tissue remodeling. The present study aimed to evaluate the expression of MMPs and their activity in the blood serum of male goats experimentally infected with caprine arthritis encephalitis virus (CAEV). Five dairy male goats, aged 3-4 years, were intravenously inoculated with CAEV Cork strain (titer: 105-6 TCID50/mL) after being tested negative for CAEV thrice at consecutive intervals of 30 days using western blot analysis and nested-PCR. The study included three stages: S1 or pre-infection stage; S2 or seroconversion stage, corresponding to the occurrence of first seroconversion; and S3 or post-seroconversion stage, corresponding to 23 weeks after seroconversion. Zymography was performed for the samples using gelatin zymography gels (12.5%), which were subjected to electrophoresis at 170V, 1A, and 300W for 50-70 min. The density of MMP-2 was found to be lower at S1 (1456.20 pixels) than that at S2 and S3 (1943.80 and 2104.40 pixels, respectively) (P < 0.05); and the density of MMP-9 was found to be lower at S3 (133.60 pixels) than that at S1 and S2 (359.60 and 370.60 pixels, respectively). The density of proMMP-2 was low at S1 and S3 (130.45 and 145.20 pixels, respectively). On the other hand, the density of proMMP-9 was statistically different between S1 and S3 (89.22 vs. 415.60 pixels). Both proMMP-2 and proMMP-9 were absent at S2. Thus, MMP-2 and MMP-9 exhibited opposite behaviors depending on the stage of infection. As the greatest activity of MMP-2 was detected at stage S3, we suggest that MMP-2 can be used as a biomarker for complementary diagnosis of acute CAEV infection. In addition, the presence of proMMP-13 can be used to indicate active viral infection. [Estudo de metaloproteinases no sangue de caprinos experimentalmente infectados pelo vírus da artrite encefalite caprina]. Resumo: A encefalite por artrite caprina é uma doença lentiviral que leva a perdas consideráveis na criação de caprinos. Na fase aguda da infecção viral, embora os anticorpos antivirais sejam produzidos pelo sistema imunológico do hospedeiro, eles não são suficientes para serem detectados por testes sorológicos. As infecções agudas começam com um período de incubação, durante o qual o genoma viral se replica e as respostas inatas do hospedeiro são iniciadas. As metaloproteinases da matriz (MMPs) são enzimas que desempenham um papel importante nos processos fisiológicos e patológicos da remodelação tecidual. O presente estudo teve como objetivo avaliar a expressão de MMPs e sua atividade no soro sanguíneo de reprodutores caprinos infectados experimentalmente pelo vírus da encefalite por artrite caprina (CAEV). Cinco machos caprinos, com idades entre 3-4 anos, foram inoculadas por via intravenosa com a cepa de CAEV Cork (título: 105-6 TCID50/mL) após serem testados negativamente para CAEV três vezes em intervalos consecutivos de 30 dias usando a análise de western blot e nested-PCR. O estudo incluiu três etapas: estágio S1 ou pré-infecção; S2 ou estágio de soroconversão, correspondente à ocorrência de primeira soroconversão; e S3 ou pós-soroconversão, correspondendo a 23 semanas após a soroconversão. A zimografia foi realizada para as amostras utilizando gel de gelatina (12,5%), que foram submetidos à eletroforese em 170V, 1A e 300W por 50-70 min. Verificou-se que a densidade de MMP-2 era menor em S1 (1456,20 pixels) do que em S2 e S3 (1943,80 e 2104,40 pixels, respectivamente) (P < 0,05); e a densidade de MMP-9 foi menor em S3 (133,60 pixels) do que em S1 e S2 (359,60 e 370,60 pixels, respectivamente). A densidade do proMMP-2 era baixa em S1 e S3 (130,45 e 145,20 pixels, respectivamente). Por outro lado, a densidade do proMMP-9 foi estatisticamente diferente entre S1 e S3 (89,22 vs. 415,60 pixels). ProMMP-2 e proMMP-9 estavam ausentes no S2. Assim, MMP-2 e MMP-9 exibiram comportamentos opostos, dependendo do estágio da infecção. Como a maior atividade da MMP-2 foi detectada no estágio S3, sugerimos que a MMP-2 possa ser usada como biomarcador para o diagnóstico complementar de infecção aguda por CAEV. Além disso, a presença de proMMP-13 pode ser usada para indicar infecção viral ativa.
650 $aBrazil
650 $aCaprine arthritis encephalitis virus
650 $aCaprine arthritis-encephalitis
650 $aDisease diagnosis
650 $aGoat diseases
650 $aGoats
650 $aInfectious diseases
650 $aLentivirus
650 $aSemiarid zones
650 $aSmall ruminants
650 $aCaprino
650 $aDiagnostico
650 $aDoença Animal
650 $aProtease
650 $aVirose Animal
653 $aCAEV
653 $aCaprine arthritis encephalit virus
653 $aEncefalite artrite caprina
653 $aExperimental infection
653 $aInfecção experimental
653 $aMMPs
653 $aZimografia
653 $aZymography
700 1 $aELOY, A. M. X.
700 1 $aPINHEIRO, R. R.
700 1 $aPEIXOTO, R. M.
700 1 $aLIMA, A. M. C.
700 1 $aBARROSO, M. L. da S.
700 1 $aFONSECA, L. M.
773 $tSemina: Ciências Agrárias, Londrina$gv. 41, n. 6, p. 3165-3176, 2020. Suplemento 2.
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Embrapa Caprinos e Ovinos (CNPC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
30/11/2005 |
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Data da última atualização: |
05/10/2007 |
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Autoria: |
MESQUITA, C. M.; HANNA, M. A.; COSTA, N. P. |
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Título: |
Self-propelled prototype soybean harvester. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Transactions of the Asae, St. Joseph, v. 48, n. 4, p. 1301-1310, 2005. |
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Idioma: |
Inglês |
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Conteúdo: |
A four-row self-propelled prototype of a new and simplified concept soybean harvester was built and fied tested as part of a major project acrried out for several years. The prototype threshes soybeans from standing, uncut plants using impact energy provided by plastic fingers mounted on opposite rotating shafts, and uses a pneumatic system for capturing, separating, cleaning, and transporting the beans. Compared to ten different combines from each of three brands with conventional tangentially fed threshing systems, and ten different combines from a brand with an axial-flow threshing system, the prototype-harvested seeds were significantly better with respect to broken/split beans and seed coat damage than all the combines with tangentially fed threshing systems. The amounts of material-other-than-grain (MOG) in the sample harvested with the prototype were significantly less than for all conventional brands. The seed vigor of the sample from the prototype was significantly better than those from one of the combine brands with a tangentially fed threshing system. Invisible mechanical damage, germination, and grain field losses were not statistically different between the prototype and all combine brands evaluated. The overall results confirmed the better quality of the collected beans, including that obtained with previous versions of the prototype. It also showed that the harvesting module, initially designed for small and medium-size farms, was adaptable to a sel-propelled unit and therefore to larger-scale operations. MenosA four-row self-propelled prototype of a new and simplified concept soybean harvester was built and fied tested as part of a major project acrried out for several years. The prototype threshes soybeans from standing, uncut plants using impact energy provided by plastic fingers mounted on opposite rotating shafts, and uses a pneumatic system for capturing, separating, cleaning, and transporting the beans. Compared to ten different combines from each of three brands with conventional tangentially fed threshing systems, and ten different combines from a brand with an axial-flow threshing system, the prototype-harvested seeds were significantly better with respect to broken/split beans and seed coat damage than all the combines with tangentially fed threshing systems. The amounts of material-other-than-grain (MOG) in the sample harvested with the prototype were significantly less than for all conventional brands. The seed vigor of the sample from the prototype was significantly better than those from one of the combine brands with a tangentially fed threshing system. Invisible mechanical damage, germination, and grain field losses were not statistically different between the prototype and all combine brands evaluated. The overall results confirmed the better quality of the collected beans, including that obtained with previous versions of the prototype. It also showed that the harvesting module, initially designed for small and medium-size farms, was adaptable to a sel-propelled... Mostrar Tudo |
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Thesagro: |
Colheita; Dano Mecânico; Soja. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02020naa a2200181 a 4500
001 1468604
005 2007-10-05
008 2005 bl --- 0-- u #d
100 1 $aMESQUITA, C. M.
245 $aSelf-propelled prototype soybean harvester.
260 $c2005
520 $aA four-row self-propelled prototype of a new and simplified concept soybean harvester was built and fied tested as part of a major project acrried out for several years. The prototype threshes soybeans from standing, uncut plants using impact energy provided by plastic fingers mounted on opposite rotating shafts, and uses a pneumatic system for capturing, separating, cleaning, and transporting the beans. Compared to ten different combines from each of three brands with conventional tangentially fed threshing systems, and ten different combines from a brand with an axial-flow threshing system, the prototype-harvested seeds were significantly better with respect to broken/split beans and seed coat damage than all the combines with tangentially fed threshing systems. The amounts of material-other-than-grain (MOG) in the sample harvested with the prototype were significantly less than for all conventional brands. The seed vigor of the sample from the prototype was significantly better than those from one of the combine brands with a tangentially fed threshing system. Invisible mechanical damage, germination, and grain field losses were not statistically different between the prototype and all combine brands evaluated. The overall results confirmed the better quality of the collected beans, including that obtained with previous versions of the prototype. It also showed that the harvesting module, initially designed for small and medium-size farms, was adaptable to a sel-propelled unit and therefore to larger-scale operations.
650 $aColheita
650 $aDano Mecânico
650 $aSoja
700 1 $aHANNA, M. A.
700 1 $aCOSTA, N. P.
773 $tTransactions of the Asae, St. Joseph$gv. 48, n. 4, p. 1301-1310, 2005.
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Embrapa Soja (CNPSO) |
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