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| 11. |  | MACRI, T. H. M.; HAYASHIDA, R.; SILVA, D. M. da; BUENO, A. de F. Injúrias causadas pela broca-das-axilas Crocidosema aporema (Lepidoptera: Tortricidae) em soja Bt. In: CONGRESSO BRASILEIRO DE SOJA, 9., 2022, Foz do iguaçu, PR. Desafios para a produtividade sustentável no Mercosul: resumos. Brasília, DF: Embrapa, 2022. Regina Maria Villas Bôas de Campos Leite, Adeney de Freitas Bueno, editores técnicos. resumo 82. p. 100.| Biblioteca(s): Embrapa Soja. |
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| 13. | | AUAD, A. M.; CARVALHO, C. A. de; SILVA, D. M. da; DERESZ, F. Flutuação populacional de cigarrinhas-das-pastagens em braquiária e capim-elefante. Pesquisa Agropecuária Brasileira, Brasílila, DF, v. 44, n. 9, p. 1205-1208, 2009.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Gado de Leite; Embrapa Unidades Centrais. |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
27/10/2015 |
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Data da última atualização: |
18/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
DÓRIA, M. S.; SOUSA, A. O. de; BARBOSA, C. de J.; COSTA, M. G. C.; GESTEIRA, A. da S.; SOUZA, R. M.; FREITAS, A. C. O.; PIROVANI, C. P. |
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Afiliação: |
MILENA SANTOS DÓRIA, UESC; AURIZÂNGELA OLIVEIRA DE SOUSA, UESC; CRISTIANE DE JESUS BARBOSA, CNPMF; MÁRCIO GILBERTO CARDOSO COSTA, UESC; ABELMON DA SILVA GESTEIRA, CNPMF; REGINA MARTINS SOUZA; ANA CAMILA OLIVEIRA FREITAS, UESC; CARLOS PRIMINHO PIROVANI, UESC. |
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Título: |
Citrus tristeza virus (CTV) causing proteomic and enzymatic changes in sweet orange variety "Westin". |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plos One, July, 2015. |
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ISSN: |
1932-6203 |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus Tristeza disease, caused by CTV (Citrus tristeza virus), committs citrus plantations around the world and specifically attacks phloem tissues of the plant. The virus exists as a mixture of more or less severe variants, which may or may not cause symptoms of Tristeza. The objective of this study was to analyze the changes caused by CTV in the proteome of stems of sweet orange, as well as in the activity and gene expression of antioxidant enzymes. The CTV-infected sweet orange displayed mild symptoms, which were characterized by the presence of sparse stem pitting throughout their stems. The presence of virus was confirmed by RT-PCR. Proteomic analysis by 2DE-PAGE-MS / MS revealed the identity of 40 proteins differentially expressed between CTV- infected and -non-infected samples. Of these, 33 were up-regulated and 7 were down-regulated in CTV-infected samples. Among the proteins identified stands out a specific from the virus, the coat protein. Other proteins identified are involved with oxidative stress and for this their enzymatic activity was measured. The activity of superoxide dismutase (SOD) was higher in CTV-infected samples, as catalase (CAT) showed higher activity in uninfected samples. The activity of guaiacol peroxidase (GPX) did not vary significantly between samples. However, ascorbate peroxidase (APX) was more active in the infected samples. The relative expression of the genes encoding CAT, SOD, APX and GPX was analyzed by quantitative real time PCR (RTqPCR). The CTV-infected samples showed greater accumulation of transcripts, except for the CAT gene. This gene showed higher expression in the uninfected samples. Taken together, it can be concluded that the CTV affects the protein profile and activity and gene expression of antioxidant enzymes in plants infected by this virus. MenosCitrus Tristeza disease, caused by CTV (Citrus tristeza virus), committs citrus plantations around the world and specifically attacks phloem tissues of the plant. The virus exists as a mixture of more or less severe variants, which may or may not cause symptoms of Tristeza. The objective of this study was to analyze the changes caused by CTV in the proteome of stems of sweet orange, as well as in the activity and gene expression of antioxidant enzymes. The CTV-infected sweet orange displayed mild symptoms, which were characterized by the presence of sparse stem pitting throughout their stems. The presence of virus was confirmed by RT-PCR. Proteomic analysis by 2DE-PAGE-MS / MS revealed the identity of 40 proteins differentially expressed between CTV- infected and -non-infected samples. Of these, 33 were up-regulated and 7 were down-regulated in CTV-infected samples. Among the proteins identified stands out a specific from the virus, the coat protein. Other proteins identified are involved with oxidative stress and for this their enzymatic activity was measured. The activity of superoxide dismutase (SOD) was higher in CTV-infected samples, as catalase (CAT) showed higher activity in uninfected samples. The activity of guaiacol peroxidase (GPX) did not vary significantly between samples. However, ascorbate peroxidase (APX) was more active in the infected samples. The relative expression of the genes encoding CAT, SOD, APX and GPX was analyzed by quantitative real time PCR (RTq... Mostrar Tudo |
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Palavras-Chave: |
Plant disease. |
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Thesagro: |
Doença de planta; Fruta cítrica; Vírus. |
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Thesaurus NAL: |
Citrus. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02591naa a2200277 a 4500
001 2027385
005 2023-05-18
008 2015 bl uuuu u00u1 u #d
022 $a1932-6203
100 1 $aDÓRIA, M. S.
245 $aCitrus tristeza virus (CTV) causing proteomic and enzymatic changes in sweet orange variety "Westin".$h[electronic resource]
260 $c2015
520 $aCitrus Tristeza disease, caused by CTV (Citrus tristeza virus), committs citrus plantations around the world and specifically attacks phloem tissues of the plant. The virus exists as a mixture of more or less severe variants, which may or may not cause symptoms of Tristeza. The objective of this study was to analyze the changes caused by CTV in the proteome of stems of sweet orange, as well as in the activity and gene expression of antioxidant enzymes. The CTV-infected sweet orange displayed mild symptoms, which were characterized by the presence of sparse stem pitting throughout their stems. The presence of virus was confirmed by RT-PCR. Proteomic analysis by 2DE-PAGE-MS / MS revealed the identity of 40 proteins differentially expressed between CTV- infected and -non-infected samples. Of these, 33 were up-regulated and 7 were down-regulated in CTV-infected samples. Among the proteins identified stands out a specific from the virus, the coat protein. Other proteins identified are involved with oxidative stress and for this their enzymatic activity was measured. The activity of superoxide dismutase (SOD) was higher in CTV-infected samples, as catalase (CAT) showed higher activity in uninfected samples. The activity of guaiacol peroxidase (GPX) did not vary significantly between samples. However, ascorbate peroxidase (APX) was more active in the infected samples. The relative expression of the genes encoding CAT, SOD, APX and GPX was analyzed by quantitative real time PCR (RTqPCR). The CTV-infected samples showed greater accumulation of transcripts, except for the CAT gene. This gene showed higher expression in the uninfected samples. Taken together, it can be concluded that the CTV affects the protein profile and activity and gene expression of antioxidant enzymes in plants infected by this virus.
650 $aCitrus
650 $aDoença de planta
650 $aFruta cítrica
650 $aVírus
653 $aPlant disease
700 1 $aSOUSA, A. O. de
700 1 $aBARBOSA, C. de J.
700 1 $aCOSTA, M. G. C.
700 1 $aGESTEIRA, A. da S.
700 1 $aSOUZA, R. M.
700 1 $aFREITAS, A. C. O.
700 1 $aPIROVANI, C. P.
773 $tPlos One, July, 2015.
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