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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
18/09/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
MAYER, F. Q.; REIS, E. M.; BEZERRA, A. V. A.; RODRIGUES, R. O.; MICHEL, T.; CERVA, C.; BERTAGNOLLI, A. C. |
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Afiliação: |
Fabiana Q. Mayer, Laboratório de Biologia Molecular/Instituto de Pesquisas Veterinárias Desidério Finamor/Fundação Estadual de Pesquisa Agropecuária; Emily M. dos Reis, Laboratório de Biologia Molecular/Instituto de Pesquisas Veterinárias Desidério Finamor/Fundação Estadual de Pesquisa Agropecuária; André Vinícius A. Bezerra, Laboratório de Biologia Molecular/Instituto de Pesquisas Veterinárias Desidério Finamor/Fundação Estadual de Pesquisa Agropecuária; Rogério O. Rodrigues, Laboratório de Leptospirose/Instituto de Pesquisas Veterinárias Desidério Finamor/Fundação Estadual de Pesquisa Agropecuária; Thais Michel, Laboratório de Parasitologia/Instituto de Pesquisas Veterinárias Desidério Finamor/Fundação Estadual de Pesquisa Agropecuária; Cristine Cerva, Laboratório de Biologia Molecular/Instituto de Pesquisas Veterinárias Desidério Finamor/Fundação Estadual de Pesquisa Agropecuária; Angélica C. Bertagnolli, Laboratório de Histopatologia/Instituto de Pesquisas Veterinárias Desidério Finamor/Fundação Estadual de Pesquisa Agropecuária. |
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Título: |
Nasal swab real-time PCR is not suitable for in vivo diagnosis of bovine tuberculosis. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 6, p. 49-554, jun. 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em português: A PCR em tempo real de swab nasal não é adequada para o diagnóstico in vivo de tuberculose bovina. |
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Conteúdo: |
Bovine tuberculosis (bTB) is a zoonosis causing economic losses and public health risks in many countries. The disease diagnosis in live animals is performed by intradermal tuberculin test, which is based on delayed hypersensitivity reactions. As tuberculosis has complex immune response, this test has limitations in sensitivity and specificity. This study sought to test an alternative approach for in vivo diagnosis of bovine tuberculosis, based on real-time polymerase chain reaction (PCR). DNA samples, extracted from nasal swabs of live cows, were sed for SYBR® Green real-time PCR, which is able to differentiate between Mycobacterium tuberculosis and Mycobacterium avium complexes. Statistical analysis was performed to compare the results of tuberculin test, the in vivo gold standard bTB diagnosis method, with realtime PCR, thereby determining the specificity and sensitivity of molecular method. Cervical comparative test (CCT) was performed in 238 animals, of which 193 had suitable DNA from nasal swabs for molecular analysis, as indicated by amplification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, and were included in the study. In total, 25 (10.5%) of the animals were C T reactive, of which none was positive in the molecular test. Of the 168 CCT negative animals, four were positive for M. tuberculosis complex at real time PCR from nasal swabs. The comparison of these results generated values of sensitivity and specificity of 0% and 97.6%, respe ctively; moreover, low coefficients of agreement and correlation (-0.029 and -0.049, respectively) between the results obtained with both tests were also observed. This study showed that real-time PCR from nasal swabs is not suitable for in vivo diagnosis of bovine tuberculosis; thus tuberculin skin test is still the best option for this purpose. MenosBovine tuberculosis (bTB) is a zoonosis causing economic losses and public health risks in many countries. The disease diagnosis in live animals is performed by intradermal tuberculin test, which is based on delayed hypersensitivity reactions. As tuberculosis has complex immune response, this test has limitations in sensitivity and specificity. This study sought to test an alternative approach for in vivo diagnosis of bovine tuberculosis, based on real-time polymerase chain reaction (PCR). DNA samples, extracted from nasal swabs of live cows, were sed for SYBR® Green real-time PCR, which is able to differentiate between Mycobacterium tuberculosis and Mycobacterium avium complexes. Statistical analysis was performed to compare the results of tuberculin test, the in vivo gold standard bTB diagnosis method, with realtime PCR, thereby determining the specificity and sensitivity of molecular method. Cervical comparative test (CCT) was performed in 238 animals, of which 193 had suitable DNA from nasal swabs for molecular analysis, as indicated by amplification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, and were included in the study. In total, 25 (10.5%) of the animals were C T reactive, of which none was positive in the molecular test. Of the 168 CCT negative animals, four were positive for M. tuberculosis complex at real time PCR from nasal swabs. The comparison of these results generated values of sensitivity and specificity of 0% and 97.6%, respe ctively; moreov... Mostrar Tudo |
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Palavras-Chave: |
Complexo Mycobacterium avium; Complexo Mycobacterium tuberculosis; Diagnóstico molecular; Molecular diagnosis; Nasal swab real-time PCR; Teste da tuberculina; Tuberculin test. |
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Thesagro: |
Mycobacterium Bovis. |
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Thesaurus Nal: |
Bovine tuberculosis; Mycobacterium avium complex; Mycobacterium tuberculosis complex. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163895/1/Nasal-swab-real-time-PCR.pdf
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Marc: |
LEADER 03022naa a2200337 a 4500
001 2075708
005 2017-12-29
008 2017 bl uuuu u00u1 u #d
100 1 $aMAYER, F. Q.
245 $aNasal swab real-time PCR is not suitable for in vivo diagnosis of bovine tuberculosis.$h[electronic resource]
260 $c2017
500 $aTítulo em português: A PCR em tempo real de swab nasal não é adequada para o diagnóstico in vivo de tuberculose bovina.
520 $aBovine tuberculosis (bTB) is a zoonosis causing economic losses and public health risks in many countries. The disease diagnosis in live animals is performed by intradermal tuberculin test, which is based on delayed hypersensitivity reactions. As tuberculosis has complex immune response, this test has limitations in sensitivity and specificity. This study sought to test an alternative approach for in vivo diagnosis of bovine tuberculosis, based on real-time polymerase chain reaction (PCR). DNA samples, extracted from nasal swabs of live cows, were sed for SYBR® Green real-time PCR, which is able to differentiate between Mycobacterium tuberculosis and Mycobacterium avium complexes. Statistical analysis was performed to compare the results of tuberculin test, the in vivo gold standard bTB diagnosis method, with realtime PCR, thereby determining the specificity and sensitivity of molecular method. Cervical comparative test (CCT) was performed in 238 animals, of which 193 had suitable DNA from nasal swabs for molecular analysis, as indicated by amplification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, and were included in the study. In total, 25 (10.5%) of the animals were C T reactive, of which none was positive in the molecular test. Of the 168 CCT negative animals, four were positive for M. tuberculosis complex at real time PCR from nasal swabs. The comparison of these results generated values of sensitivity and specificity of 0% and 97.6%, respe ctively; moreover, low coefficients of agreement and correlation (-0.029 and -0.049, respectively) between the results obtained with both tests were also observed. This study showed that real-time PCR from nasal swabs is not suitable for in vivo diagnosis of bovine tuberculosis; thus tuberculin skin test is still the best option for this purpose.
650 $aBovine tuberculosis
650 $aMycobacterium avium complex
650 $aMycobacterium tuberculosis complex
650 $aMycobacterium Bovis
653 $aComplexo Mycobacterium avium
653 $aComplexo Mycobacterium tuberculosis
653 $aDiagnóstico molecular
653 $aMolecular diagnosis
653 $aNasal swab real-time PCR
653 $aTeste da tuberculina
653 $aTuberculin test
700 1 $aREIS, E. M.
700 1 $aBEZERRA, A. V. A.
700 1 $aRODRIGUES, R. O.
700 1 $aMICHEL, T.
700 1 $aCERVA, C.
700 1 $aBERTAGNOLLI, A. C.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 6, p. 49-554, jun. 2017.
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