|
|
Registro Completo |
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
04/01/2021 |
Data da última atualização: |
08/01/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SANTOS, C. M. dos; NASCIMENTO, W. B. A.; NASCIMENRO, B. P. do; SCHWAB, S.; BALDANI, J. I.; VIDAL, M. S. |
Afiliação: |
Carlos M. Dos Santos, UFRRJ; Wiglison B. A. Nascimento, UFRRK; Bruna P. do Nascimento, UFRRJ; STEFAN SCHWAB, CNPAB; JOSE IVO BALDANI, CNPAB; MARCIA SOARES VIDAL, CNPAB. |
Título: |
Temporal assessment of root and shoot colonization of elephant grass (Pennisetum purpureum Schum.) host seedlings by Gluconacetobacter diazotrophicus strain LP343. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Microbiological Research, v. 244, e126651, 2021. |
DOI: |
10.1016/j.micres.2020.126651 |
Idioma: |
Inglês |
Conteúdo: |
Gluconacetobacter diazotrophicus is a species of great agronomic potential due to its growth-promotion traits. Its colonization process in different plants has been reported. However, there have been no studies regarding its structural colonization in elephant grass. This is a fast-growing C4-Poaceae plant, and its application in Brazil is mainly aimed at feeding dairy cattle, due to its high nutritional value. Also, in the last decade, this grass has been applied in the production of biofuels. The present study aimed to monitor the colonization process of strain LP343 of G. diazotrophicus inoculated in elephant grass seedlings of PCEA genotype, by using a mCherry-tagged bacterium. Samples of roots and shoots collected at different periods were visualized by confocal laser-scanning microscopy. The colony-counting assay was used to compare the number of cells recovered in different niches and a qPCR was performed for the quantification of endophytic cells in root and shoot tissues. Results suggested that the strain LP343 quickly recognized the PCEA roots as host, attached to the elephant grass roots at 6 h, and 7 days after inoculation were able to colonize the xylem vessels of roots and shoots of elephant grass. This study advances our knowledge about the colonization process of G. diazotrophicus species in elephant grass, contributing to future studies involving the plant-bacteria interaction cultivated under gnotobiotic conditions. |
Palavras-Chave: |
Endophytic colonization; FBN. |
Thesaurus Nal: |
Genotype. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02215naa a2200229 a 4500 001 2128964 005 2021-01-08 008 2021 bl uuuu u00u1 u #d 024 7 $a10.1016/j.micres.2020.126651$2DOI 100 1 $aSANTOS, C. M. dos 245 $aTemporal assessment of root and shoot colonization of elephant grass (Pennisetum purpureum Schum.) host seedlings by Gluconacetobacter diazotrophicus strain LP343.$h[electronic resource] 260 $c2021 520 $aGluconacetobacter diazotrophicus is a species of great agronomic potential due to its growth-promotion traits. Its colonization process in different plants has been reported. However, there have been no studies regarding its structural colonization in elephant grass. This is a fast-growing C4-Poaceae plant, and its application in Brazil is mainly aimed at feeding dairy cattle, due to its high nutritional value. Also, in the last decade, this grass has been applied in the production of biofuels. The present study aimed to monitor the colonization process of strain LP343 of G. diazotrophicus inoculated in elephant grass seedlings of PCEA genotype, by using a mCherry-tagged bacterium. Samples of roots and shoots collected at different periods were visualized by confocal laser-scanning microscopy. The colony-counting assay was used to compare the number of cells recovered in different niches and a qPCR was performed for the quantification of endophytic cells in root and shoot tissues. Results suggested that the strain LP343 quickly recognized the PCEA roots as host, attached to the elephant grass roots at 6 h, and 7 days after inoculation were able to colonize the xylem vessels of roots and shoots of elephant grass. This study advances our knowledge about the colonization process of G. diazotrophicus species in elephant grass, contributing to future studies involving the plant-bacteria interaction cultivated under gnotobiotic conditions. 650 $aGenotype 653 $aEndophytic colonization 653 $aFBN 700 1 $aNASCIMENTO, W. B. A. 700 1 $aNASCIMENRO, B. P. do 700 1 $aSCHWAB, S. 700 1 $aBALDANI, J. I. 700 1 $aVIDAL, M. S. 773 $tMicrobiological Research$gv. 244, e126651, 2021.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Agrobiologia (CNPAB) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
09/04/2015 |
Data da última atualização: |
30/01/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
COSENDEY-KEZENLEITE, R. I. J.; OLIVEIRA, F. C. R. de; FRAZÃO-TEIXEIRA, E.; DUBEY, J. P.; SOUZA, G. N. de; FERREIRA, A. M. R.; LILENBAUM, W. |
Afiliação: |
RACHEL INGRID JULIBONI COSENDEY-KEZENLEITE, UFF; FRANCISCO CARLOS RODRIGUES DE OLIVEIRA, UENF; EDWARDS FRAZÃO-TEIXEIRA, UENF; JITENDER PRAKASH DUBEY, Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville; GUILHERME NUNES DE SOUZA, CNPGL; ANA MARIA REIS FERREIRA, UFF; WALTER LILENBAUM, UFF. |
Título: |
Occurrence and risk factors associated to Toxoplasma gondii infection in sheep from Rio de Janeiro, Brazil. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Tropical Animal Health and Production, v. 46, n. 8, p. 1463-1466, 2014. |
Idioma: |
Inglês |
Thesaurus NAL: |
Brazil; risk factors; serology; sheep; toxoplasmosis. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 00754naa a2200241 a 4500 001 2013138 005 2024-01-30 008 2014 bl uuuu u00u1 u #d 100 1 $aCOSENDEY-KEZENLEITE, R. I. J. 245 $aOccurrence and risk factors associated to Toxoplasma gondii infection in sheep from Rio de Janeiro, Brazil.$h[electronic resource] 260 $c2014 650 $aBrazil 650 $arisk factors 650 $aserology 650 $asheep 650 $atoxoplasmosis 700 1 $aOLIVEIRA, F. C. R. de 700 1 $aFRAZÃO-TEIXEIRA, E. 700 1 $aDUBEY, J. P. 700 1 $aSOUZA, G. N. de 700 1 $aFERREIRA, A. M. R. 700 1 $aLILENBAUM, W. 773 $tTropical Animal Health and Production$gv. 46, n. 8, p. 1463-1466, 2014.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|