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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
12/09/2014 |
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Data da última atualização: |
13/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FREIRE, I. S.; MIRANDA-VILELA, A. L.; FASCINELI, M. L.; OLIVEIRA FILHO, E. C. de; MARTINS, E. S.; PONTES, R. G. M. S. de; GRISOLIA, C. K. |
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Afiliação: |
EDUARDO CYRINO DE OLIVEIRA FILHO, CPAC; ROSE GOMES MONNERAT SOLON DE PONTES, CENARGEN. |
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Título: |
Genotoxic evaluation in Oreochromis niloticus (Fish: Characidae) of recombinant spore-crystal complexes Cry1Ia, Cry10Aa and Cry1Ba6 from Bacillus thuringiensis. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Ecotoxicology, v. 23, p. 267-272, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
Bioinsecticides from Bacillus thuringiensis (Bt) are widely used around the world in biological control against larval stages of many insect species. Bt has been considered a biopesticide that is highly specific to different orders of insects, non-polluting and harmless to humans and other vertebrates, thus becoming a viable alternative for combating agricultural pests and insect vectors of diseases. The family of Bt d-endotoxins are crystal-protein inclusions showing toxicity to insects? midgut, causing cell lysis leading to starvation, septicemia and death. The aim of this study is to evaluate the genotoxic potential of recombinant Bt spore?crystals expressing Cry1Ia, Cry10Aa and Cry1Ba6 on peripheral erythrocyte cells of Oreochromis niloticus, through comet assay, micronucleus (MN) test and nuclear abnormalities (NA) analysis. Fish (n = 10/group) were exposed for 96 h at 107 spores 30 l-1, 108 spores 30 l-1 or 109 spores 30 l-1 of Bt spore?crystals. Cry1Ia showed a significant increase in comet cells at levels 1 and 2, but not at levels 3 and 4, so it was not mutagenic nor did it induce MN or NA. These three spore?crystals showed some fish toxicity at only the highest exposure level, which normally does not occur in the field. |
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Palavras-Chave: |
Avaliação genotóxica; Biopesticida; Comet assay; Micronucleus; Spore-crystal. |
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Thesagro: |
Bacillus Thuringiensis; Pesticida. |
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Thesaurus Nal: |
Biopesticides; Genotoxicity. |
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Categoria do assunto: |
--
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/180007/1/Freire2014-Article-GenotoxicEvaluationInOreochrom.pdf
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Marc: |
LEADER 02166naa a2200301 a 4500
001 1994844
005 2023-03-13
008 2014 bl uuuu u00u1 u #d
100 1 $aFREIRE, I. S.
245 $aGenotoxic evaluation in Oreochromis niloticus (Fish$bCharacidae) of recombinant spore-crystal complexes Cry1Ia, Cry10Aa and Cry1Ba6 from Bacillus thuringiensis.
260 $c2014
520 $aBioinsecticides from Bacillus thuringiensis (Bt) are widely used around the world in biological control against larval stages of many insect species. Bt has been considered a biopesticide that is highly specific to different orders of insects, non-polluting and harmless to humans and other vertebrates, thus becoming a viable alternative for combating agricultural pests and insect vectors of diseases. The family of Bt d-endotoxins are crystal-protein inclusions showing toxicity to insects? midgut, causing cell lysis leading to starvation, septicemia and death. The aim of this study is to evaluate the genotoxic potential of recombinant Bt spore?crystals expressing Cry1Ia, Cry10Aa and Cry1Ba6 on peripheral erythrocyte cells of Oreochromis niloticus, through comet assay, micronucleus (MN) test and nuclear abnormalities (NA) analysis. Fish (n = 10/group) were exposed for 96 h at 107 spores 30 l-1, 108 spores 30 l-1 or 109 spores 30 l-1 of Bt spore?crystals. Cry1Ia showed a significant increase in comet cells at levels 1 and 2, but not at levels 3 and 4, so it was not mutagenic nor did it induce MN or NA. These three spore?crystals showed some fish toxicity at only the highest exposure level, which normally does not occur in the field.
650 $aBiopesticides
650 $aGenotoxicity
650 $aBacillus Thuringiensis
650 $aPesticida
653 $aAvaliação genotóxica
653 $aBiopesticida
653 $aComet assay
653 $aMicronucleus
653 $aSpore-crystal
700 1 $aMIRANDA-VILELA, A. L.
700 1 $aFASCINELI, M. L.
700 1 $aOLIVEIRA FILHO, E. C. de
700 1 $aMARTINS, E. S.
700 1 $aPONTES, R. G. M. S. de
700 1 $aGRISOLIA, C. K.
773 $tEcotoxicology$gv. 23, p. 267-272, 2014.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| 1. |  | CORRÊA-FERREIRA, B. S. Amostragem de pragas da soja. In: HOFFMANN-CAMPO, C. B.; CORRÊA-FERREIRA, B. S.; MOSCARDI, F. Soja: manejo integrado de insetos e outros Artrópodes-praga. Brasília, DF: Embrapa, 2012. p. 631-672.| Tipo: Capítulo em Livro Técnico-Científico |
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