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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
12/11/2019 |
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Data da última atualização: |
12/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COELHO, S. V. B.; ROSA, S. D. V. F. da; FANTAZZINI, T. B.; SILVA, L. C. |
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Afiliação: |
Stefânia Vilas Boas Coelho, Universidade Federal de Lavras - UFLA; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; Tatiana Botelho Fantazzini, Universidade Federal de Lavras - UFLA; Luciano Coutinho Silva, Universidade Federal da Paraíba - UFPB. |
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Título: |
Cryopreservation of Coffea canephora Pierre ex A. Froehner seeds: importance of drying rate and moisture content. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Australian Journal of Crop Science, v.13, n. 8, p. 1335-1342, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Seeds of the Coffea canephora species are considered more recalcitrant than those of the Coffea arabica species. They tolerate partial dehydration and they cannot be stored in conventional seed banks at -18ºC because they are sensitive to freezing temperatures. Cryopreservation is a reliable method for storing seeds with these characteristics for long periods. However, preliminary studies are necessary to determine ideal storage conditions. The aim of this study was to investigate the ideal physical and physiological conditions for cryopreservation of Coffea canephora seeds to reduce seed mortality caused by the formation of intracellular ice crystals and to avoid cell damage caused by excessive desiccation. Seeds were subjected to rapid drying in silica gel and slow drying in saturated NaCl solution to moisture contents of 0.20, 0.25, and 0.28 g.g-¹ (dry basis), followed by direct immersion in liquid nitrogen for rapid freezing. Physiological and biochemical analyses were performed to evaluate seed quality before and after cryopreservation. Rapid drying of Coffea canephora seeds to values near 0.20 g.g-1 (db) does not cause reduction in physiological quality. Moisture content of 0.25 g.g-¹ brings about greater survival of Coffea canephora seeds after cryopreservation. Drying rate affects the physiological quality of cryopreserved Coffea canephora seeds, and rapid drying in silica gel is more favorable than slow drying in a saturated NaCl solution. The activity of the enzymes catalase, esterase, glutamic oxaloacetic transaminase, and polyphenol oxidase are indicators of seed quality for Coffea canephora seeds subjected to cryopreservation. MenosSeeds of the Coffea canephora species are considered more recalcitrant than those of the Coffea arabica species. They tolerate partial dehydration and they cannot be stored in conventional seed banks at -18ºC because they are sensitive to freezing temperatures. Cryopreservation is a reliable method for storing seeds with these characteristics for long periods. However, preliminary studies are necessary to determine ideal storage conditions. The aim of this study was to investigate the ideal physical and physiological conditions for cryopreservation of Coffea canephora seeds to reduce seed mortality caused by the formation of intracellular ice crystals and to avoid cell damage caused by excessive desiccation. Seeds were subjected to rapid drying in silica gel and slow drying in saturated NaCl solution to moisture contents of 0.20, 0.25, and 0.28 g.g-¹ (dry basis), followed by direct immersion in liquid nitrogen for rapid freezing. Physiological and biochemical analyses were performed to evaluate seed quality before and after cryopreservation. Rapid drying of Coffea canephora seeds to values near 0.20 g.g-1 (db) does not cause reduction in physiological quality. Moisture content of 0.25 g.g-¹ brings about greater survival of Coffea canephora seeds after cryopreservation. Drying rate affects the physiological quality of cryopreserved Coffea canephora seeds, and rapid drying in silica gel is more favorable than slow drying in a saturated NaCl solution. The activity of the enzyme... Mostrar Tudo |
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Palavras-Chave: |
Antioxidant enzymes; Drying in silica gel; Ice crystals; Physiological quality; Rapid drying. |
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Thesaurus Nal: |
Recalcitrant seeds. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/204558/1/Cryopreservation-of-Coffea-canephora-Pierre.pdf
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Marc: |
LEADER 02404naa a2200229 a 4500
001 2114203
005 2019-11-12
008 2019 bl uuuu u00u1 u #d
100 1 $aCOELHO, S. V. B.
245 $aCryopreservation of Coffea canephora Pierre ex A. Froehner seeds$bimportance of drying rate and moisture content.$h[electronic resource]
260 $c2019
520 $aSeeds of the Coffea canephora species are considered more recalcitrant than those of the Coffea arabica species. They tolerate partial dehydration and they cannot be stored in conventional seed banks at -18ºC because they are sensitive to freezing temperatures. Cryopreservation is a reliable method for storing seeds with these characteristics for long periods. However, preliminary studies are necessary to determine ideal storage conditions. The aim of this study was to investigate the ideal physical and physiological conditions for cryopreservation of Coffea canephora seeds to reduce seed mortality caused by the formation of intracellular ice crystals and to avoid cell damage caused by excessive desiccation. Seeds were subjected to rapid drying in silica gel and slow drying in saturated NaCl solution to moisture contents of 0.20, 0.25, and 0.28 g.g-¹ (dry basis), followed by direct immersion in liquid nitrogen for rapid freezing. Physiological and biochemical analyses were performed to evaluate seed quality before and after cryopreservation. Rapid drying of Coffea canephora seeds to values near 0.20 g.g-1 (db) does not cause reduction in physiological quality. Moisture content of 0.25 g.g-¹ brings about greater survival of Coffea canephora seeds after cryopreservation. Drying rate affects the physiological quality of cryopreserved Coffea canephora seeds, and rapid drying in silica gel is more favorable than slow drying in a saturated NaCl solution. The activity of the enzymes catalase, esterase, glutamic oxaloacetic transaminase, and polyphenol oxidase are indicators of seed quality for Coffea canephora seeds subjected to cryopreservation.
650 $aRecalcitrant seeds
653 $aAntioxidant enzymes
653 $aDrying in silica gel
653 $aIce crystals
653 $aPhysiological quality
653 $aRapid drying
700 1 $aROSA, S. D. V. F. da
700 1 $aFANTAZZINI, T. B.
700 1 $aSILVA, L. C.
773 $tAustralian Journal of Crop Science$gv.13, n. 8, p. 1335-1342, 2019.
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Registro original: |
Embrapa Café (CNPCa) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
09/08/2019 |
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Data da última atualização: |
02/10/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
CAMARGO, A. P.; SOUZA, R. S. C. de; COSTA, P. de B.; GERHARDT, I. R.; DANTE, R. A.; TEODORO, G. S.; ABRAHÃO, A.; LAMBERS, H.; CARAZZOLLE, M. F.; HUNTEMANN, M.; CLUM, A.; FOSTER, B.; FOSTER, B.; ROUX, S.; PALANIAPPAN, K.; VARGHESE, N.; MUKHERJEE, S.; REDDY, T. B. K.; DAUM, C.; COPELAND, A.; CHENM U, M. A.; IVANOVA, N. N.; KYRPIDES, N. C.; PENNACCHIO, C.; ELOE-FADROSH, E. A.; ARRUDA, P.; OLIVEIRA, R. S. |
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Afiliação: |
ANTONIO PEDRO CAMARGO, Unicamp; RAFAEL SOARES CORREA DE SOUZA, Unicamp; PATRÍCIA DE BRITTO COSTA, Unicamp, University of Western Australia; ISABEL RODRIGUES GERHARDT, CNPTIA, Unicamp; RICARDO AUGUSTO DANTE, CNPTIA, Unicamp; GRAZIELLE SALES TEODORO, UFPA; ANNA ABRAHÃO, Unicamp, University of Western Australia; HANS LAMBERS, University of Western Australia; MARCELO FALSARELLA CARAZZOLLE, Unicamp; MARCEL HUNTEMANN, Department of Energy Joint Genome Institute; ALICIA CLUM, Department of Energy Joint Genome Institute; BRIAN FOSTER, Department of Energy Joint Genome Institute; BRYCE FOSTER, Department of Energy Joint Genome Institute; SIMON ROUX, Department of Energy Joint Genome Institute; KRISHNAVENI PALANIAPPAN, Department of Energy Joint Genome Institute; NEHA VARGHESE, Department of Energy Joint Genome Institute; SUPRATIM MUKHERJEE, Department of Energy Joint Genome Institute; T. B. K. REDDY, Department of Energy Joint Genome Institute; CHRIS DAUM, Department of Energy Joint Genome Institute; ALEX COPELAND, Department of Energy Joint Genome Institute; I.-MIN A. CHEN, Department of Energy Joint Genome Institute; NATALIA N. IVANOVA, Department of Energy Joint Genome Institute; NIKOS C. KYRPIDES, Department of Energy Joint Genome Institute; CHRISTA PENNACCHIO, Department of Energy Joint Genome Institute; EMILEY A. ELOE-FADROSH, Department of Energy Joint Genome Institute; PAULO ARRUDA, Unicamp; RAFAEL SILVA OLIVEIRA, Unicamp, University of Western Australia. |
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Título: |
Microbiomes of Velloziaceae from phosphorus-impoverished soils of the campos rupestres, a biodiversity hotspot. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Scientific Data, v. 6, p. 1-11, 2019. |
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DOI: |
https://doi.org/10.1038/s41597-019-0141-3 |
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Idioma: |
Inglês |
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Notas: |
Article number: 140. |
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Conteúdo: |
The rocky, seasonally-dry and nutrient-impoverished soils of the Brazilian campos rupestres impose severe growth-limiting conditions on plants. Species of a dominant plant family, Velloziaceae, are highly specialized to low-nutrient conditions and seasonal water availability of this environment, where phosphorus (P) is the key limiting nutrient. Despite plant-microbe associations playing critical roles in stressful ecosystems, the contribution of these interactions in the campos rupestres remains poorly studied. Here we present the first microbiome data of Velloziaceae spp. thriving in contrasting substrates of campos rupestres. We assessed the microbiomes of Vellozia epidendroides, which occupies shallow patches of soil, and Barbacenia macrantha, growing on exposed rocks. The prokaryotic and fungal profiles were assessed by rRNA barcode sequencing of epiphytic and endophytic compartments of roots, stems, leaves and surrounding soil/rocks. We also generated root and substrate (rock/soil)-associated metagenomes of each plant species. We foresee that these data will contribute to decipher how the microbiome contributes to plant functioning in the campos rupestres, and to unravel new strategies for improved crop productivity in stressful environments. |
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Palavras-Chave: |
Campos rupestres brasileiros; Condições extremas; DNA sequencing; Microbioma de plantas; Sequenciamento genético; Soils; Solos; Stressful environments. |
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Thesagro: |
Microrganismo. |
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Thesaurus NAL: |
Microbiome; Microorganisms; Velloziaceae. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/200538/1/AP-Microbiomes-Velloziaceae.pdf
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Marc: |
LEADER 02937naa a2200601 a 4500
001 2111238
005 2019-10-02
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/s41597-019-0141-3$2DOI
100 1 $aCAMARGO, A. P.
245 $aMicrobiomes of Velloziaceae from phosphorus-impoverished soils of the campos rupestres, a biodiversity hotspot.$h[electronic resource]
260 $c2019
500 $aArticle number: 140.
520 $aThe rocky, seasonally-dry and nutrient-impoverished soils of the Brazilian campos rupestres impose severe growth-limiting conditions on plants. Species of a dominant plant family, Velloziaceae, are highly specialized to low-nutrient conditions and seasonal water availability of this environment, where phosphorus (P) is the key limiting nutrient. Despite plant-microbe associations playing critical roles in stressful ecosystems, the contribution of these interactions in the campos rupestres remains poorly studied. Here we present the first microbiome data of Velloziaceae spp. thriving in contrasting substrates of campos rupestres. We assessed the microbiomes of Vellozia epidendroides, which occupies shallow patches of soil, and Barbacenia macrantha, growing on exposed rocks. The prokaryotic and fungal profiles were assessed by rRNA barcode sequencing of epiphytic and endophytic compartments of roots, stems, leaves and surrounding soil/rocks. We also generated root and substrate (rock/soil)-associated metagenomes of each plant species. We foresee that these data will contribute to decipher how the microbiome contributes to plant functioning in the campos rupestres, and to unravel new strategies for improved crop productivity in stressful environments.
650 $aMicrobiome
650 $aMicroorganisms
650 $aVelloziaceae
650 $aMicrorganismo
653 $aCampos rupestres brasileiros
653 $aCondições extremas
653 $aDNA sequencing
653 $aMicrobioma de plantas
653 $aSequenciamento genético
653 $aSoils
653 $aSolos
653 $aStressful environments
700 1 $aSOUZA, R. S. C. de
700 1 $aCOSTA, P. de B.
700 1 $aGERHARDT, I. R.
700 1 $aDANTE, R. A.
700 1 $aTEODORO, G. S.
700 1 $aABRAHÃO, A.
700 1 $aLAMBERS, H.
700 1 $aCARAZZOLLE, M. F.
700 1 $aHUNTEMANN, M.
700 1 $aCLUM, A.
700 1 $aFOSTER, B.
700 1 $aFOSTER, B.
700 1 $aROUX, S.
700 1 $aPALANIAPPAN, K.
700 1 $aVARGHESE, N.
700 1 $aMUKHERJEE, S.
700 1 $aREDDY, T. B. K.
700 1 $aDAUM, C.
700 1 $aCOPELAND, A.
700 1 $aCHENM U, M. A.
700 1 $aIVANOVA, N. N.
700 1 $aKYRPIDES, N. C.
700 1 $aPENNACCHIO, C.
700 1 $aELOE-FADROSH, E. A.
700 1 $aARRUDA, P.
700 1 $aOLIVEIRA, R. S.
773 $tScientific Data$gv. 6, p. 1-11, 2019.
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Embrapa Agricultura Digital (CNPTIA) |
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