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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
19/09/2022 |
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Data da última atualização: |
19/09/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MATTOS, L. M.; REIS, F. C.; RACANICCI, A. M. C.; PIVATO, I.; TONELLI, G. S. S. S.; BÁO, S. N.; MARTINS, C. F. |
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Afiliação: |
LUCIANA MIRANDA MATTOS; FILIPE CARNEIRO REIS; ALINE M.C. RACANICCI; IVO PIVATO; GUILHERME S.S.S. TONELLI; SÔNIA NAIR BÁO; CARLOS FREDERICO MARTINS, CPAC. |
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Título: |
Freezing for the Future: obtaining fibroblast samples from deceased wild mammals for the Brazilian Cerrado Germplasm Bank. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Biopreservation and Biobanking, ahead of print, 2022. |
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DOI: |
DOI: 10.1089/bio.2022.0039 |
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Idioma: |
Inglês |
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Conteúdo: |
We isolated and further characterized fibroblasts obtained from postmortem skin biopsies of three different Brazilian wild species (Chrysocyon brachyurus?maned wolf, Cerdocyon thous?crab-eating fox, Mazama gouazoubira?brown brocket deer). The effects of two cryoprotectants, 10% dimethyl sulfoxide (DMSO) and 5% dimethylformamide (DMF), were assessed to determine the most efficient cryopreservation protocol. Such an investigation promotes the creation of germplasm banks, using samples that would otherwise be rejected and permanently lost following the death of the animals. We utilized animal corpses that were involved in highway accidents, found dead in the natural environment, or referred to us from the veterinary hospital at the Brası ́lia Zoo. Fibroblasts from C. brachyurus specimens presented a delay in cell growth in Dulbecco?s modified Eagle?s medium in relation to other species. This observation is a limiting factor for the future storage of cells from this species. Differences in cellular morphology were observed between C. brachyurus, C. thous, and M. goua- zoubira, presenting branched, fusiform, and spherical forms, respectively. The cryoprotective solution con- taining 10% DMSO was more efficient than 5% DMF medium in preserving the viability of fibroblasts of the three species ( p < 0.05). After defining the best cryopreservation solution, a germplasm bank was successfully formed. This biological reservoir is configured as the first germplasm bank containing somatic cells and gametes of wild mammals of the Cerrado biome of Brazil. This material will be used for future characterization of the species and multiplication by means of nuclear transfer cloning. MenosWe isolated and further characterized fibroblasts obtained from postmortem skin biopsies of three different Brazilian wild species (Chrysocyon brachyurus?maned wolf, Cerdocyon thous?crab-eating fox, Mazama gouazoubira?brown brocket deer). The effects of two cryoprotectants, 10% dimethyl sulfoxide (DMSO) and 5% dimethylformamide (DMF), were assessed to determine the most efficient cryopreservation protocol. Such an investigation promotes the creation of germplasm banks, using samples that would otherwise be rejected and permanently lost following the death of the animals. We utilized animal corpses that were involved in highway accidents, found dead in the natural environment, or referred to us from the veterinary hospital at the Brası ́lia Zoo. Fibroblasts from C. brachyurus specimens presented a delay in cell growth in Dulbecco?s modified Eagle?s medium in relation to other species. This observation is a limiting factor for the future storage of cells from this species. Differences in cellular morphology were observed between C. brachyurus, C. thous, and M. goua- zoubira, presenting branched, fusiform, and spherical forms, respectively. The cryoprotective solution con- taining 10% DMSO was more efficient than 5% DMF medium in preserving the viability of fibroblasts of the three species ( p < 0.05). After defining the best cryopreservation solution, a germplasm bank was successfully formed. This biological reservoir is configured as the first germplasm bank contain... Mostrar Tudo |
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Thesagro: |
Animal Selvagem; Banco de Germoplasma; Biotecnologia; Criopreservação. |
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Thesaurus Nal: |
Biotechnology; Cryopreservation; Germplasm; Wild animals. |
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Categoria do assunto: |
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Marc: |
LEADER 02613naa a2200301 a 4500
001 2146609
005 2022-09-19
008 2022 bl uuuu u00u1 u #d
024 7 $aDOI: 10.1089/bio.2022.0039$2DOI
100 1 $aMATTOS, L. M.
245 $aFreezing for the Future$bobtaining fibroblast samples from deceased wild mammals for the Brazilian Cerrado Germplasm Bank.$h[electronic resource]
260 $c2022
520 $aWe isolated and further characterized fibroblasts obtained from postmortem skin biopsies of three different Brazilian wild species (Chrysocyon brachyurus?maned wolf, Cerdocyon thous?crab-eating fox, Mazama gouazoubira?brown brocket deer). The effects of two cryoprotectants, 10% dimethyl sulfoxide (DMSO) and 5% dimethylformamide (DMF), were assessed to determine the most efficient cryopreservation protocol. Such an investigation promotes the creation of germplasm banks, using samples that would otherwise be rejected and permanently lost following the death of the animals. We utilized animal corpses that were involved in highway accidents, found dead in the natural environment, or referred to us from the veterinary hospital at the Brası ́lia Zoo. Fibroblasts from C. brachyurus specimens presented a delay in cell growth in Dulbecco?s modified Eagle?s medium in relation to other species. This observation is a limiting factor for the future storage of cells from this species. Differences in cellular morphology were observed between C. brachyurus, C. thous, and M. goua- zoubira, presenting branched, fusiform, and spherical forms, respectively. The cryoprotective solution con- taining 10% DMSO was more efficient than 5% DMF medium in preserving the viability of fibroblasts of the three species ( p < 0.05). After defining the best cryopreservation solution, a germplasm bank was successfully formed. This biological reservoir is configured as the first germplasm bank containing somatic cells and gametes of wild mammals of the Cerrado biome of Brazil. This material will be used for future characterization of the species and multiplication by means of nuclear transfer cloning.
650 $aBiotechnology
650 $aCryopreservation
650 $aGermplasm
650 $aWild animals
650 $aAnimal Selvagem
650 $aBanco de Germoplasma
650 $aBiotecnologia
650 $aCriopreservação
700 1 $aREIS, F. C.
700 1 $aRACANICCI, A. M. C.
700 1 $aPIVATO, I.
700 1 $aTONELLI, G. S. S. S.
700 1 $aBÁO, S. N.
700 1 $aMARTINS, C. F.
773 $tBiopreservation and Biobanking, ahead of print, 2022.
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Registro original: |
Embrapa Cerrados (CPAC) |
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