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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
01/06/2023 |
Data da última atualização: |
01/06/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
DORTA, S. DE O.; ATTÍLIO, L. B.; ZANARDI, O. Z.; LOPES, J. R. S.; MACHADO, M. A.; ASTUA, J. de F. |
Afiliação: |
SÍLVIA DE OLIVEIRA DORTA, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; LÍSIA BORGES ATTÍLIO, INSTITUTO AGRONÔMICO DE CAMPINAS; ODIMAR ZANUZO ZANARDI, INSTITUTO FEDERAL DE SANTA CATARINA; JOÃO ROBERTO SPOTTI LOPES, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; MARCOS ANTONIO MACHADO, INSTITUTO AGRONÔMICO DE CAMPINAS; JULIANA DE FREITAS ASTUA, CNPMF. |
Título: |
Genetic transformation of 'Hamlin' and 'Valencia' sweet orange plants expressing the cry11A gene of Bacillus thuringiensis as an additional tool for the management of Diaphorina citri (Hemiptera: Liviidae). |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Journal of Biotechnolog, v.68, May, p.60-70, 2023. |
ISSN: |
0168-1656 |
DOI: |
https://doi.org/10.1016/j.jbiotec.2023.04.007 |
Idioma: |
Inglês |
Conteúdo: |
The Asian citrus psyllid (ACP) Diaphorina citri Kuwayama (Hemiptera: Liviidae) is the vector of Candidatus Liberibacter spp., the bacteria associated with huanglongbing (HLB), the most devastating disease of citrus worldwide. HLB management has heavily relied on insecticide applications to control the ACP, although there are efforts towards more sustainable alternatives. In previous work, our group assessed the potential bioactivity of different strains of Bacillus thuringiensis (Eubacteriales: Bacillaceae) (Bt) containing cry/cyt genes as feasible tools to control ACP nymphs. Here, we report an attempt to use the cry11A gene from Bt to produce transgenic sweet orange plants using two promoters. For the genetic transformation, ?Hamlin? and ?Valencia? sweet orange seedlings were used as sources of explants. Transgenic plants were detected by polymerase chain reaction (PCR) with specific primers, and the transgene copy number was confirmed by Southern blot analyses. Transcript expression levels were determined by qPCR. Mortality assays of D. citri nymphs were carried out in a greenhouse, and the effect of the events tested ranged from 22% to 43% at the end of the five-day exposure period. To our knowledge, this is the first manuscript reporting the production of citrus plants expressing the Bt cry11A gene for the management of D. citri nymphs. |
Thesagro: |
Laranja; Melhoramento Vegetal. |
Thesaurus Nal: |
Oranges. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02204naa a2200241 a 4500 001 2154159 005 2023-06-01 008 2023 bl uuuu u00u1 u #d 022 $a0168-1656 024 7 $ahttps://doi.org/10.1016/j.jbiotec.2023.04.007$2DOI 100 1 $aDORTA, S. DE O. 245 $aGenetic transformation of 'Hamlin' and 'Valencia' sweet orange plants expressing the cry11A gene of Bacillus thuringiensis as an additional tool for the management of Diaphorina citri (Hemiptera$bLiviidae).$h[electronic resource] 260 $c2023 520 $aThe Asian citrus psyllid (ACP) Diaphorina citri Kuwayama (Hemiptera: Liviidae) is the vector of Candidatus Liberibacter spp., the bacteria associated with huanglongbing (HLB), the most devastating disease of citrus worldwide. HLB management has heavily relied on insecticide applications to control the ACP, although there are efforts towards more sustainable alternatives. In previous work, our group assessed the potential bioactivity of different strains of Bacillus thuringiensis (Eubacteriales: Bacillaceae) (Bt) containing cry/cyt genes as feasible tools to control ACP nymphs. Here, we report an attempt to use the cry11A gene from Bt to produce transgenic sweet orange plants using two promoters. For the genetic transformation, ?Hamlin? and ?Valencia? sweet orange seedlings were used as sources of explants. Transgenic plants were detected by polymerase chain reaction (PCR) with specific primers, and the transgene copy number was confirmed by Southern blot analyses. Transcript expression levels were determined by qPCR. Mortality assays of D. citri nymphs were carried out in a greenhouse, and the effect of the events tested ranged from 22% to 43% at the end of the five-day exposure period. To our knowledge, this is the first manuscript reporting the production of citrus plants expressing the Bt cry11A gene for the management of D. citri nymphs. 650 $aOranges 650 $aLaranja 650 $aMelhoramento Vegetal 700 1 $aATTÍLIO, L. B. 700 1 $aZANARDI, O. Z. 700 1 $aLOPES, J. R. S. 700 1 $aMACHADO, M. A. 700 1 $aASTUA, J. de F. 773 $tJournal of Biotechnolog$gv.68, May, p.60-70, 2023.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
06/01/2011 |
Data da última atualização: |
07/01/2011 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
CERQUEIRA-SILVA, C. B. M.; CARDOSO-SILVA, C. B.; CONCEIÇÃO, L. D. H. C. S. da; PEREIRA, A. S.; OLIVEIRA, A. C.; CORRÊA, R. X. |
Afiliação: |
C. B. M. CERQUEIRA-SILVA, UESB; C. B. CARDOSO-SILVA, UNICAMP; LEO DUC HAA CARSON SCHWARTZHAUPT DA CONCEIÇÃO, CPAC; A. S. PEREIRA, UESC; A. C. OLIVEIRA, UESB; R. X. CORRÊA, UESC. |
Título: |
Genetic diversity in wild species of passion fruit (Passiflora trintae) based on molecular markers. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 9, n. 4, p. 2123-2130, 2010. |
Idioma: |
Inglês |
Palavras-Chave: |
Passiflora trintae. |
Thesagro: |
Marcador Molecular. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00630naa a2200193 a 4500 001 1871768 005 2011-01-07 008 2010 bl uuuu u00u1 u #d 100 1 $aCERQUEIRA-SILVA, C. B. M. 245 $aGenetic diversity in wild species of passion fruit (Passiflora trintae) based on molecular markers. 260 $c2010 650 $aMarcador Molecular 653 $aPassiflora trintae 700 1 $aCARDOSO-SILVA, C. B. 700 1 $aCONCEIÇÃO, L. D. H. C. S. da 700 1 $aPEREIRA, A. S. 700 1 $aOLIVEIRA, A. C. 700 1 $aCORRÊA, R. X. 773 $tGenetics and Molecular Research$gv. 9, n. 4, p. 2123-2130, 2010.
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