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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
24/01/2011 |
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Data da última atualização: |
03/06/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
KULCHESKI, F. R.; MARCELINO, F. C.; NEPOMUCENO, A. L.; ABDELNOOR, R. V.; MARGIS, R. |
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Afiliação: |
F. R. KULCHESKI, Universidade Federal do Rio Grande do Sul, UFRGS; FRANCISMAR CORREA MARCELINO, CNPSO; ALEXANDRE LIMA NEPOMUCENO, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; R. MARGIS, Universidade Federal do Rio Grande do Sul, UFRGS. |
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Título: |
The use of microRNAs as universal reference genes in quantitative PCR. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE GENÉTICA, 56., 2010, Guarujá. Resumos... [Curitiba]: UFPR, 2010. p. 312. |
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Idioma: |
Português |
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Conteúdo: |
Reverse transcription quantitative real-time PCR (RT-qPCR) is a robust and widely applied technique used to investigate gene expression. However, for correct analysis and interpretation of results, the choice of a suitable gene to use as an internal control is a crucial factor. These genes, such as housekeeping genes, should have a constant expression level in different tissues and across different conditions. The advances in genome sequencing have provided high-throughput gene expression analysis and have contributed to the identification of new genes, including microRNAs (miRNAs). The miRNAs are fundamental regulatory genes of eukaryotic genomes, acting on several biological functions. In this study, miRNA expression stability was investigated in different soybean tissues and genotypes as well as after abiotic or biotic stress treatments. The present study represents the first investigation into the suitability of miRNAs as housekeeping genes in plants. The transcript stability of ten miRNAs was compared to those of six previously reported housekeeping genes for the soybean. In this study, we provide evidence that miRNA expression stability can be greater than the expression stability of protein-coding genes. In addition, we conclude that miRNAs are optimal reference genes not only. |
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Thesagro: |
Soja. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/25959/1/GP-312.pdf
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Marc: |
LEADER 01837nam a2200169 a 4500
001 1874076
005 2011-06-03
008 2010 bl uuuu u00u1 u #d
100 1 $aKULCHESKI, F. R.
245 $aThe use of microRNAs as universal reference genes in quantitative PCR.
260 $aIn: CONGRESSO BRASILEIRO DE GENÉTICA, 56., 2010, Guarujá. Resumos... [Curitiba]: UFPR, 2010. p. 312.$c2010
520 $aReverse transcription quantitative real-time PCR (RT-qPCR) is a robust and widely applied technique used to investigate gene expression. However, for correct analysis and interpretation of results, the choice of a suitable gene to use as an internal control is a crucial factor. These genes, such as housekeeping genes, should have a constant expression level in different tissues and across different conditions. The advances in genome sequencing have provided high-throughput gene expression analysis and have contributed to the identification of new genes, including microRNAs (miRNAs). The miRNAs are fundamental regulatory genes of eukaryotic genomes, acting on several biological functions. In this study, miRNA expression stability was investigated in different soybean tissues and genotypes as well as after abiotic or biotic stress treatments. The present study represents the first investigation into the suitability of miRNAs as housekeeping genes in plants. The transcript stability of ten miRNAs was compared to those of six previously reported housekeeping genes for the soybean. In this study, we provide evidence that miRNA expression stability can be greater than the expression stability of protein-coding genes. In addition, we conclude that miRNAs are optimal reference genes not only.
650 $aSoja
700 1 $aMARCELINO, F. C.
700 1 $aNEPOMUCENO, A. L.
700 1 $aABDELNOOR, R. V.
700 1 $aMARGIS, R.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
18/10/2011 |
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Data da última atualização: |
02/07/2012 |
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Autoria: |
COLLARES, D. G.; VASCONCELLOS, J. H. |
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Afiliação: |
DANIELA GARCIA COLLARES, CNPAE; JOSE HEITOR VASCONCELLOS, CNPMS. |
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Título: |
O sorgo sacarino na mídia. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Agroenergia em revista, ano 2, n. 3, p. 48-49, agosto 2011. |
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Idioma: |
Português |
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Palavras-Chave: |
Mídia; Sorgo sacarino. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/44191/1/Revista-Agroenergia-3-1433.pdf
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Marc: |
LEADER 00408naa a2200145 a 4500
001 1903325
005 2012-07-02
008 2011 bl uuuu u00u1 u #d
100 1 $aCOLLARES, D. G.
245 $aO sorgo sacarino na mídia.$h[electronic resource]
260 $c2011
653 $aMídia
653 $aSorgo sacarino
700 1 $aVASCONCELLOS, J. H.
773 $tAgroenergia em revista, ano 2$gn. 3, p. 48-49, agosto 2011.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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