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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
04/11/2020 |
Data da última atualização: |
04/11/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ESPÍNDOLA, J. P.; BALBINOTT, N.; GRESSLER, L. T.; MACHADO, G.; KLEIN, C. S.; REBELATTO, R.; MARTÍN, C. B. G.; KREUTZ, L. C.; SCHRYVERS, A. B.; FRANDOLOSO, R. |
Afiliação: |
JÚLIA PIRES ESPÍNDOLA, UPF; NATÁLIA BALBINOTT, UPF; LETÍCIA TREVISAN GRESSLER, UPF; GUSTAVO MACHADO, North Carolina State University; CATIA SILENE KLEIN, CNPSA; RAQUEL REBELATTO, CNPSA; CÉSAR BERNARDO GUTIÉRREZ MARTÍN, University of León; LUIZ CARLOS KREUTZ, UPF; ANTHONY BERNARD SCHRYVERS, University of Calgary; RAFAEL FRANDOLOSO, UPF. |
Título: |
Molecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glassers disease outbreaks in Brazil. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
PeerJ, v. 7, n. e6817, 2019. |
DOI: |
http://doi.org/10.7717/peerj.6817 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Glässer's disease (GD) is an important infectious disease of swine caused by Haemophilus (Glaesserella) parasuis. Vaccination with inactivated whole cell vaccines is the major approach for prevention of H. parasuis infection worldwide, but the immunity induced is predominantly against the specific polysaccharide capsule. As a consequence, the available vaccines may not induce adequate protection against the field strains, when the capsules present in the vaccine strains are different from those in strains isolated from the farms. Therefore, it is crucial to map H. parasuis serovars associated with regional outbreaks so that appropriate bacterin vaccines can be developed and distributed for prevention of infection. In this study, 459 H. parasuis field strains isolated from different Glässer's disease outbreaks that occurred in 10 different Brazilian States were analyzed for serotype using PCR-based approaches. Surprisingly, non-typeable (NT) strains were the second most prevalent group of field strains and along with serovars 4, 5 and 1 comprised more than 70% of the isolates. A PCRbased approach designed to amplify the entire polysaccharide capsule locus revealed 9 different band patterns in the NT strains, and 75% of the NT strains belonged to three clusters, suggesting that a number of new serovars are responsible for a substantial proportion of disease. These results indicate that commercially available vaccines in Brazil do not cover the most prevalent H. parasuis serovars associated with GD. Disease mapping, Haemophilus parasuis, Typification, Serovars, Vaccines, Brazil Microbiology, Veterinary Medicine MenosAbstract: Glässer's disease (GD) is an important infectious disease of swine caused by Haemophilus (Glaesserella) parasuis. Vaccination with inactivated whole cell vaccines is the major approach for prevention of H. parasuis infection worldwide, but the immunity induced is predominantly against the specific polysaccharide capsule. As a consequence, the available vaccines may not induce adequate protection against the field strains, when the capsules present in the vaccine strains are different from those in strains isolated from the farms. Therefore, it is crucial to map H. parasuis serovars associated with regional outbreaks so that appropriate bacterin vaccines can be developed and distributed for prevention of infection. In this study, 459 H. parasuis field strains isolated from different Glässer's disease outbreaks that occurred in 10 different Brazilian States were analyzed for serotype using PCR-based approaches. Surprisingly, non-typeable (NT) strains were the second most prevalent group of field strains and along with serovars 4, 5 and 1 comprised more than 70% of the isolates. A PCRbased approach designed to amplify the entire polysaccharide capsule locus revealed 9 different band patterns in the NT strains, and 75% of the NT strains belonged to three clusters, suggesting that a number of new serovars are responsible for a substantial proportion of disease. These results indicate that commercially available vaccines in Brazil do not cover the most prevalent H. paras... Mostrar Tudo |
Palavras-Chave: |
Brasil; Disease mapping; Serovares; Serovars; Tipificação; Typification. |
Thesagro: |
Microbiologia; Vacina; Veterinária. |
Thesaurus Nal: |
Brazil; Haemophilus parasuis; Microbiology; Vaccines; Veterinary medicine. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02802naa a2200409 a 4500 001 2126299 005 2020-11-04 008 2019 bl uuuu u00u1 u #d 024 7 $ahttp://doi.org/10.7717/peerj.6817$2DOI 100 1 $aESPÍNDOLA, J. P. 245 $aMolecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glassers disease outbreaks in Brazil.$h[electronic resource] 260 $c2019 520 $aAbstract: Glässer's disease (GD) is an important infectious disease of swine caused by Haemophilus (Glaesserella) parasuis. Vaccination with inactivated whole cell vaccines is the major approach for prevention of H. parasuis infection worldwide, but the immunity induced is predominantly against the specific polysaccharide capsule. As a consequence, the available vaccines may not induce adequate protection against the field strains, when the capsules present in the vaccine strains are different from those in strains isolated from the farms. Therefore, it is crucial to map H. parasuis serovars associated with regional outbreaks so that appropriate bacterin vaccines can be developed and distributed for prevention of infection. In this study, 459 H. parasuis field strains isolated from different Glässer's disease outbreaks that occurred in 10 different Brazilian States were analyzed for serotype using PCR-based approaches. Surprisingly, non-typeable (NT) strains were the second most prevalent group of field strains and along with serovars 4, 5 and 1 comprised more than 70% of the isolates. A PCRbased approach designed to amplify the entire polysaccharide capsule locus revealed 9 different band patterns in the NT strains, and 75% of the NT strains belonged to three clusters, suggesting that a number of new serovars are responsible for a substantial proportion of disease. These results indicate that commercially available vaccines in Brazil do not cover the most prevalent H. parasuis serovars associated with GD. Disease mapping, Haemophilus parasuis, Typification, Serovars, Vaccines, Brazil Microbiology, Veterinary Medicine 650 $aBrazil 650 $aHaemophilus parasuis 650 $aMicrobiology 650 $aVaccines 650 $aVeterinary medicine 650 $aMicrobiologia 650 $aVacina 650 $aVeterinária 653 $aBrasil 653 $aDisease mapping 653 $aSerovares 653 $aSerovars 653 $aTipificação 653 $aTypification 700 1 $aBALBINOTT, N. 700 1 $aGRESSLER, L. T. 700 1 $aMACHADO, G. 700 1 $aKLEIN, C. S. 700 1 $aREBELATTO, R. 700 1 $aMARTÍN, C. B. G. 700 1 $aKREUTZ, L. C. 700 1 $aSCHRYVERS, A. B. 700 1 $aFRANDOLOSO, R. 773 $tPeerJ$gv. 7, n. e6817, 2019.
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Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Agroindústria Tropical. |
Data corrente: |
23/08/2017 |
Data da última atualização: |
15/02/2018 |
Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
Autoria: |
LEITAO, R. C.; GEHRING, T. A.; CAVALCANTE, W. de A.; FREITAS, I. B. F.; COLARES, A. S.; HAANDEL, A. C. van; ANGENENT, L. T.; SANTAELLA, S. T. |
Afiliação: |
RENATO CARRHA LEITAO, CNPAT; TITO AUGUSTO GEHRING, Engenheiro sanitaristae ambiental, doutor em Engenharia Civil e Ambiental, pós-doutando e bolsista BJT na Embrapa Agroindústria Tropical; WILLAME de ARAÚJO CAVALCANTE, Cientista ambental, mestre em Engenharia civil pela Universidade de São Paulo, Escola de Engenharia de São Carlos; ISABELE BAIMA FERREIRA FREITAS, Cientista ambiental, mestranda no Depto. de Hidráulica e Saneamento da Escola de Engenharia de São Carlos; ALDO SOUZA COLARES, Graduando em Engenharia Química pela Universidade Federal do Ceará, bolsista PIBIC, Embrapa Agroindústria Tropical; ADRIANUS CORNELIUS van HAANDEL, Engenheiro químico, doutado em Engenharia Civil, Professor Adjunto da Universidade Federal de Campina Grande; LARGUS T. ANGENENT, Cientista ambiental, doutor em Engenharia Ambiental, professor na Universidade de Cornell Depto. de Engenharia Biológica e Ambiental EUA; SANDRA TÉDDE SANTAELLA, Química doutora em Hidráulica e Saneamento, professora associada da Universidade Federal do Ceará. |
Título: |
Produção biológica de ácido caproico em um reator em escala de laboratório. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Fortaleza: Embrapa Agroindústria Tropical, 2017. |
Páginas: |
6 p. |
Série: |
(Embrapa Agroindústria Tropical. Comunicado técnico, 229). |
Idioma: |
Português |
Palavras-Chave: |
Ácido capróico. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162982/1/COT17003.pdf
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Marc: |
LEADER 00701nam a2200217 a 4500 001 2074384 005 2018-02-15 008 2017 bl uuuu u0uu1 u #d 100 1 $aLEITAO, R. C. 245 $aProdução biológica de ácido caproico em um reator em escala de laboratório.$h[electronic resource] 260 $aFortaleza: Embrapa Agroindústria Tropical$c2017 300 $a6 p. 490 $a(Embrapa Agroindústria Tropical. Comunicado técnico, 229). 653 $aÁcido capróico 700 1 $aGEHRING, T. A. 700 1 $aCAVALCANTE, W. de A. 700 1 $aFREITAS, I. B. F. 700 1 $aCOLARES, A. S. 700 1 $aHAANDEL, A. C. van 700 1 $aANGENENT, L. T. 700 1 $aSANTAELLA, S. T.
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