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| Registros recuperados : 30 | |
| 21. |  | COELHO, S. V. B.; ROSA, S. D. V. F. da; CLEMENTE, A. da C. S.; PEREIRA, C. C.; FIGUEIREDO, M. A. de; REIS, L. V. Tolerance of Coffea arabica L. seeds to sub zero temperatures. Ciência e Agrotecnologia, v. 41, n. 3, p. 312-321, May/Jun. 2017. Título em português: Tolerância de sementes de Coffea arabica L. à temperaturas sub zero.| Biblioteca(s): Embrapa Café. |
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| 22. | | ABREU, G. F. de; ROSA, S. D. V. F. da; CAIXETA, F.; CLEMENTE, A. da C. S.; PEREIRA, C. C.; COELHO, S. V. B. Colorimetria em grãos de café submetidos a diferentes métodos de processamento e armazenados em ambiente resfriado. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 8., 2013, Salvador. Sustentabilidade e inclusão Social. Brasília, DF: Embrapa Café, 2013.| Biblioteca(s): Embrapa Café. |
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| 23. | | ABREU, G. F.; ROSA, S. D. V. F. da; COELHO, S. V. B.; PEREIRA, C. C.; MALTA, M. R.; FANTAZZINI, T. B.; VILELA, A. L. Influence of hulling and storage conditions on maintaining coffee quality. Anais da Academia Brasileira de Ciências, v. 95, n. 4, 2023. 12 p.| Biblioteca(s): Embrapa Café. |
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| 24. | | SANTOS, F. C. dos; ROSA, S. D. V. F. da; COELHO, S. V. B.; CLEMENTE, A. C. S.; FANTAZZINI, T. B.; PEREIRA, C. C.; CAIXETA, F.; FÁVARIS, N. A. B.; BAÚTE, J. L. GA3 and ABI3 gene expression in coffee seeds during maturation. Genetics and Molecular Research, v. 18, n. 3, 2019| Biblioteca(s): Embrapa Café. |
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| 25. | | FANTAZZINI, T. B.; ROSA, S. D. V. F. da; CARVALHO, G. R.; LISKA, G. R.; CARVALHO, M. L. M. de; COELHO, S. V. B.; CIRILLO, M. A.; RIBEIRO, F. A. S. Correlation between historical data of the germination test and of the tetrazolium test in coffee seeds by GAMLSS. Seed Science and Technology, v. 48, n. 2, p. 179-188, Apr. 2020.| Biblioteca(s): Embrapa Café. |
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| 26. | | FANTAZZINI, T. B.; ROSA, S. D. V. F. da; COELHO, S. V. B.; SOUSA, P. H. A.; PEREIRA, D. de S.; VILELA, A. L. de O.; RIBEIRO, F. A. S. Applicability of the tetrazolium test to coffee seeds with different types and levels of stresses. Coffee Science, v. 16, e161904, 2022.| Biblioteca(s): Embrapa Café. |
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| 27. | | CARVALHO, M. V. de; MORAES, P. I. C.; COELHO, S. V. B.; FIGUEIREDO, M. A. de; VILELA, A. L. O.; SOUZA, A. C. de; ROSA, S. D. V. F. da. Accelerated aging test for coffee seeds requires higher temperature. Australian Journal of Crop Science, v. 17, n. 6, 2023. 8 p.| Biblioteca(s): Embrapa Café. |
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| 28. | | COELHO, S. V. B.; ROSA, S. D. V. F. da; LACERDA, L. N. C.; CLEMENTE, A. da C. S.; SILVA, L. C.; FANTAZZINI, T. B.; RIBEIRO, F. S.; CASTRO, E. de M. Ultrastructural damage in coffee seeds exposed to drying and to subzero (°C) temperatures. Coffee Science, v. 15, e151760, p. 1-8, 2020.| Biblioteca(s): Embrapa Café. |
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| 29. | | SOUZA, A. C. de; COSTA, M. C.; FIGUEIREDO, M. A. de; PEREIRA, C. C.; COELHO, S. V. B.; VILELA, A. L. O.; PEREIRA, D. de S.; ROSA, S. D. V. F. da. Steps of cryopreservation of coffee seeds: physiological responses and antioxidant systems. Ciência Rural, v. 54, n. 2, e20220480, 2024. 12 p. Título em português: Etapas da criopreservação de sementes de café: respostas fisiológicas e de sistemas antioxidantes.| Biblioteca(s): Embrapa Café. |
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| 30. | | CARVALHO, M. H. de; ROSA, S. D. V. F. da; COELHO, S. V. B.; GUIMARÃES, C. C.; MARTINS, R. de S.; CLEMENTE, A. da C. S.; PAIVA, L. V. Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality. Acta Scientiarum: Agronomy, v. 45, e56908, 2023.| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 30 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
12/11/2019 |
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Data da última atualização: |
12/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SANTOS, F. C. dos; ROSA, S. D. V. F. da; COELHO, S. V. B.; CLEMENTE, A. C. S.; FANTAZZINI, T. B.; PEREIRA, C. C.; CAIXETA, F.; FÁVARIS, N. A. B.; BAÚTE, J. L. |
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Afiliação: |
Hehagro Pesquisa; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; Universidade Federal de Lavras - UFLA/Laboratório Central de Sementes/DAG; Grupo Fertilágua; Universidade Federal de Lavras - UFLA/Laboratório Central de Sementes/DAG; Universidade Federal de Lavras - UFLA/Laboratório Central de Sementes/DAG; Universidade Federal de Lavras - UFLA/Laboratório Central de Sementes/DAG; Universidade Federal de Lavras - UFLA/Laboratório Central de Sementes/DAG; Universidade Federal de Lavras - UFLA/Laboratório Central de Sementes/DAG. |
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Título: |
GA3 and ABI3 gene expression in coffee seeds during maturation. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 18, n. 3, 2019 |
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Idioma: |
Inglês |
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Conteúdo: |
During maturation in seeds, metabolic processes dependent on gene expression are controlled by hormones, including abscisic acid and gibberellin. These hormones determine dormancy or the capacity for germination in seeds and may have different expression levels in the endosperm and embryo of coffee seeds. We quantified gene expression in the biosynthetic pathway of gibberellin and abscisic acid in different parts of Coffea arabica L. seeds in pre and post physiological maturity, in order to better understand the germination mechanisms of this species. Coffee fruits were harvested at green, yellowish-green, cherry, over-ripe and dry stages. For studies of gene expression, intact seeds were used in addition to endosperm and isolated embryos. The RNA from different tissues was extracted and treated with DNAse to synthesize cDNA. Transition levels of CaGA3 (gibberellin) and CaABI3 (abscisic acid) genes were quantified with qRT-PCR, using specific primers for coffee. Intact seeds at each phenological stage were submitted to germination tests, to evaluate the physiological quality of the seeds. Seed harvested at green and dry stages showed lower hysiological quality when compared to the other maturation stages, and germination at the green stage was close to zero. Greatest expression of CaGA3 and CaABI3 occurs in the endosperm of coffee seeds in cherry and over-ripe stages, with better physiological performance. There are differences in CaABI3 and CaGA3 gene expression in embryos and endosperm of the coffee seeds. MenosDuring maturation in seeds, metabolic processes dependent on gene expression are controlled by hormones, including abscisic acid and gibberellin. These hormones determine dormancy or the capacity for germination in seeds and may have different expression levels in the endosperm and embryo of coffee seeds. We quantified gene expression in the biosynthetic pathway of gibberellin and abscisic acid in different parts of Coffea arabica L. seeds in pre and post physiological maturity, in order to better understand the germination mechanisms of this species. Coffee fruits were harvested at green, yellowish-green, cherry, over-ripe and dry stages. For studies of gene expression, intact seeds were used in addition to endosperm and isolated embryos. The RNA from different tissues was extracted and treated with DNAse to synthesize cDNA. Transition levels of CaGA3 (gibberellin) and CaABI3 (abscisic acid) genes were quantified with qRT-PCR, using specific primers for coffee. Intact seeds at each phenological stage were submitted to germination tests, to evaluate the physiological quality of the seeds. Seed harvested at green and dry stages showed lower hysiological quality when compared to the other maturation stages, and germination at the green stage was close to zero. Greatest expression of CaGA3 and CaABI3 occurs in the endosperm of coffee seeds in cherry and over-ripe stages, with better physiological performance. There are differences in CaABI3 and CaGA3 gene expression in embryos ... Mostrar Tudo |
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Palavras-Chave: |
QRT-PCR. |
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Thesagro: |
Coffea Arábica. |
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Thesaurus NAL: |
Abscisic acid; Gibberellic acid. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/204559/1/Ga3-and-ABI3-gene-expression.pdf
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Marc: |
LEADER 02279naa a2200265 a 4500
001 2114204
005 2019-11-12
008 2019 bl uuuu u00u1 u #d
100 1 $aSANTOS, F. C. dos
245 $aGA3 and ABI3 gene expression in coffee seeds during maturation.$h[electronic resource]
260 $c2019
520 $aDuring maturation in seeds, metabolic processes dependent on gene expression are controlled by hormones, including abscisic acid and gibberellin. These hormones determine dormancy or the capacity for germination in seeds and may have different expression levels in the endosperm and embryo of coffee seeds. We quantified gene expression in the biosynthetic pathway of gibberellin and abscisic acid in different parts of Coffea arabica L. seeds in pre and post physiological maturity, in order to better understand the germination mechanisms of this species. Coffee fruits were harvested at green, yellowish-green, cherry, over-ripe and dry stages. For studies of gene expression, intact seeds were used in addition to endosperm and isolated embryos. The RNA from different tissues was extracted and treated with DNAse to synthesize cDNA. Transition levels of CaGA3 (gibberellin) and CaABI3 (abscisic acid) genes were quantified with qRT-PCR, using specific primers for coffee. Intact seeds at each phenological stage were submitted to germination tests, to evaluate the physiological quality of the seeds. Seed harvested at green and dry stages showed lower hysiological quality when compared to the other maturation stages, and germination at the green stage was close to zero. Greatest expression of CaGA3 and CaABI3 occurs in the endosperm of coffee seeds in cherry and over-ripe stages, with better physiological performance. There are differences in CaABI3 and CaGA3 gene expression in embryos and endosperm of the coffee seeds.
650 $aAbscisic acid
650 $aGibberellic acid
650 $aCoffea Arábica
653 $aQRT-PCR
700 1 $aROSA, S. D. V. F. da
700 1 $aCOELHO, S. V. B.
700 1 $aCLEMENTE, A. C. S.
700 1 $aFANTAZZINI, T. B.
700 1 $aPEREIRA, C. C.
700 1 $aCAIXETA, F.
700 1 $aFÁVARIS, N. A. B.
700 1 $aBAÚTE, J. L.
773 $tGenetics and Molecular Research$gv. 18, n. 3, 2019
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