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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
24/10/2011 |
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Data da última atualização: |
19/02/2013 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
MUNIZ, F. L.; SILVEIRA, R. DA.; CAMPOS, Z.; MAGNUSSON, W. E.; HRBEK, T.; FARIAS, I. P. |
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Afiliação: |
FABIO L. MUNIZ, UNIVERSIDADE FEDERAL DO AMAZONAS; RONIS DA SILVEIRA, UNIVERSIDADE FEDERAL DO AMAZONAS; ZILCA MARIA DA SILVA CAMPOS, CPAP; WILLIAM E. MAGNUSSON, INSTITUTO NACIONAL DE PESQUISAS DA AMAZÔNIA; TOMAS HRBEK, UNIVERSIDADE FEDERAL DO AMAZONAS; IZENI P. FARIAS, UNIVERSIDADE FEDERAL DO AMAZONAS. |
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Título: |
Multiple paternity in the Black Caiman (Melanosuchus niger) population in the Anavilhanas National Park, Brazilian Amazonia. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Amphibia Reptilia, v.32, n.3, p. 428-434, aug., 2011. |
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Idioma: |
Português |
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Conteúdo: |
The formation of dominance hierarchies in which the female mates with a large dominant male is common among crocodilians. However, there is the possibility of polyandry, in which females mate with multiple partners during a single breeding season and generate offspring with multiple paternity. In the present study, eight pairs of heterologous primers developed for Alligator mississippiensis and Caiman latirostris were used to determine whether multiple paternity exists in the Black Caiman, Melanosuchus niger. For such, we analyzed 34 Black Caiman offspring from the Anavilhanas Archipelago in the Negro River (state of Amazonas, Brazil). The specimens came from six groups, each containing five or six hatchlings. Paternity exclusion and genetic identity indices were calculated to test the robustness of the microsatellite loci. Simple allele counts and maximum likelihood estimation of family clusters were used to determine the likelihood of occurrence of multiple paternity. Among the eight loci tested, five were effective at determining paternity, with paternity exclusion values close to 1.0 (QC = 0.92) and genetic identity values close to zero (IC < 0.01). Using the simple allele count, six cases of multiple paternity were detected and confirmed in three hatchling groups by four different microsatellite loci. However, maximum likelihood analysis indicated multiple paternity in all the groups analyzed, with five family clusters identified in one hatchling group alone. Considering that this species is listed according to IUCN as Lower Risk/Conservation Dependent, our results have direct conservation implications. Multiple paternity increases effective population size by maintaining genetic variation, and thus could be an important mechanism to maintain genetic diversity in isolated local populations. MenosThe formation of dominance hierarchies in which the female mates with a large dominant male is common among crocodilians. However, there is the possibility of polyandry, in which females mate with multiple partners during a single breeding season and generate offspring with multiple paternity. In the present study, eight pairs of heterologous primers developed for Alligator mississippiensis and Caiman latirostris were used to determine whether multiple paternity exists in the Black Caiman, Melanosuchus niger. For such, we analyzed 34 Black Caiman offspring from the Anavilhanas Archipelago in the Negro River (state of Amazonas, Brazil). The specimens came from six groups, each containing five or six hatchlings. Paternity exclusion and genetic identity indices were calculated to test the robustness of the microsatellite loci. Simple allele counts and maximum likelihood estimation of family clusters were used to determine the likelihood of occurrence of multiple paternity. Among the eight loci tested, five were effective at determining paternity, with paternity exclusion values close to 1.0 (QC = 0.92) and genetic identity values close to zero (IC < 0.01). Using the simple allele count, six cases of multiple paternity were detected and confirmed in three hatchling groups by four different microsatellite loci. However, maximum likelihood analysis indicated multiple paternity in all the groups analyzed, with five family clusters identified in one hatchling group alone. Considerin... Mostrar Tudo |
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Palavras-Chave: |
Microsatellites. |
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Thesaurus Nal: |
Alligatoridae; Melanosuchus niger. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 02474naa a2200217 a 4500
001 1903858
005 2013-02-19
008 2011 bl --- 0-- u #d
100 1 $aMUNIZ, F. L.
245 $aMultiple paternity in the Black Caiman (Melanosuchus niger) population in the Anavilhanas National Park, Brazilian Amazonia.
260 $c2011
520 $aThe formation of dominance hierarchies in which the female mates with a large dominant male is common among crocodilians. However, there is the possibility of polyandry, in which females mate with multiple partners during a single breeding season and generate offspring with multiple paternity. In the present study, eight pairs of heterologous primers developed for Alligator mississippiensis and Caiman latirostris were used to determine whether multiple paternity exists in the Black Caiman, Melanosuchus niger. For such, we analyzed 34 Black Caiman offspring from the Anavilhanas Archipelago in the Negro River (state of Amazonas, Brazil). The specimens came from six groups, each containing five or six hatchlings. Paternity exclusion and genetic identity indices were calculated to test the robustness of the microsatellite loci. Simple allele counts and maximum likelihood estimation of family clusters were used to determine the likelihood of occurrence of multiple paternity. Among the eight loci tested, five were effective at determining paternity, with paternity exclusion values close to 1.0 (QC = 0.92) and genetic identity values close to zero (IC < 0.01). Using the simple allele count, six cases of multiple paternity were detected and confirmed in three hatchling groups by four different microsatellite loci. However, maximum likelihood analysis indicated multiple paternity in all the groups analyzed, with five family clusters identified in one hatchling group alone. Considering that this species is listed according to IUCN as Lower Risk/Conservation Dependent, our results have direct conservation implications. Multiple paternity increases effective population size by maintaining genetic variation, and thus could be an important mechanism to maintain genetic diversity in isolated local populations.
650 $aAlligatoridae
650 $aMelanosuchus niger
653 $aMicrosatellites
700 1 $aSILVEIRA, R. DA.
700 1 $aCAMPOS, Z.
700 1 $aMAGNUSSON, W. E.
700 1 $aHRBEK, T.
700 1 $aFARIAS, I. P.
773 $tAmphibia Reptilia$gv.32, n.3, p. 428-434, aug., 2011.
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Registro original: |
Embrapa Pantanal (CPAP) |
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
18/01/2022 |
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Data da última atualização: |
18/01/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
COELHO, S. V. B.; ROSA, S. D. V. F. da; LACERDA, L. N. C.; CLEMENTE, A. da C. S.; SILVA, L. C.; FANTAZZINI, T. B.; RIBEIRO, F. S.; CASTRO, E. de M. |
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Afiliação: |
STEFÂNIA VILAS BOAS COELHO, UFLA; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; LAURA NARDELLI CASTANHEIRA LACERDA, UFLA; ALINE DA CONSOLAÇÃO SAMPAIO CLEMENTE, GRUPO FERTILÁQUA; LUCIANO COUTINHO SILVA, UNIVERSIDADE FEDERAL DA PARAÍBA; TATIANA BOTELHO FANTAZZINI, CAMPEÃ AGRONEGÓCIO; FERNANDO SALES RIBEIRO, UFLA; ELISA DE MELO CASTRO, UFLA. |
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Título: |
Ultrastructural damage in coffee seeds exposed to drying and to subzero (°C) temperatures. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Coffee Science, v. 15, e151760, p. 1-8, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
During drying and freezing, protective mechanisms act to maintain seed physiological quality. Some of these mechanisms contribute to the integrity of cell membranes. The damage caused to cell membranes due to cell stress can be seen in ultrastructural studies. The aim of this study was to investigate ultrastructural changes in endosperm cells of coffee seeds brought about by drying and by exposure to low temperatures. Seeds of Coffea Arabica were dried in silica gel to moisture contents of 40, 20, and 5 % (wb) and brought to equilibrium at temperatures of 10, -20, and -86oC. Germination, vigor, and tetrazolium tests were performed for evaluation of seed physiological quality. Ultrastructural damage was analyzed by scanning electron microscopy. Coffee seeds with 40% moisture content have whole, swollen, and expanded cells, with a filled lumen and without signs of damage. The physiological and ultrastructural quality of seeds exposed to below zero temperatures with 40% moisture content is compromised. They have null germination and empty cells, indicating leakage of cell content. Drying of coffee seeds leads to uniform contraction of inner cell content. Drying of coffee seeds to 5% moisture content leads to intense contraction of cell volume, with physiological and ultrastructural damage. |
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Thesagro: |
Coffea Arábica; Dano Pelo Frio; Semente. |
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Thesaurus NAL: |
Coffea arabica var. arabica; Scanning electron microscopy; Seeds. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/230356/1/Ultrastructural-damage-in-coffee-seeds.pdf
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Marc: |
LEADER 02127naa a2200277 a 4500
001 2139148
005 2022-01-18
008 2020 bl uuuu u00u1 u #d
100 1 $aCOELHO, S. V. B.
245 $aUltrastructural damage in coffee seeds exposed to drying and to subzero (°C) temperatures.$h[electronic resource]
260 $c2020
520 $aDuring drying and freezing, protective mechanisms act to maintain seed physiological quality. Some of these mechanisms contribute to the integrity of cell membranes. The damage caused to cell membranes due to cell stress can be seen in ultrastructural studies. The aim of this study was to investigate ultrastructural changes in endosperm cells of coffee seeds brought about by drying and by exposure to low temperatures. Seeds of Coffea Arabica were dried in silica gel to moisture contents of 40, 20, and 5 % (wb) and brought to equilibrium at temperatures of 10, -20, and -86oC. Germination, vigor, and tetrazolium tests were performed for evaluation of seed physiological quality. Ultrastructural damage was analyzed by scanning electron microscopy. Coffee seeds with 40% moisture content have whole, swollen, and expanded cells, with a filled lumen and without signs of damage. The physiological and ultrastructural quality of seeds exposed to below zero temperatures with 40% moisture content is compromised. They have null germination and empty cells, indicating leakage of cell content. Drying of coffee seeds leads to uniform contraction of inner cell content. Drying of coffee seeds to 5% moisture content leads to intense contraction of cell volume, with physiological and ultrastructural damage.
650 $aCoffea arabica var. arabica
650 $aScanning electron microscopy
650 $aSeeds
650 $aCoffea Arábica
650 $aDano Pelo Frio
650 $aSemente
700 1 $aROSA, S. D. V. F. da
700 1 $aLACERDA, L. N. C.
700 1 $aCLEMENTE, A. da C. S.
700 1 $aSILVA, L. C.
700 1 $aFANTAZZINI, T. B.
700 1 $aRIBEIRO, F. S.
700 1 $aCASTRO, E. de M.
773 $tCoffee Science$gv. 15, e151760, p. 1-8, 2020.
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Embrapa Café (CNPCa) |
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