Data/hora: 18/06/2024 13:33:58

Biblioteca(s): | Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 1
Data corrente: | 19/05/2017
Data da última atualização: | 17/06/2020
Autoria: | SILVA, R. A. da; SOUZA, G.; LEMOS, L. S. L.; LOPES, U. V.; PATROCÌNIO, N. G. R. B.; ALVES, R. M.; MARCELLINO, L. H.; CLEMENT, D.; MICHELI, F.; GRAMACHO, K. P.
Afiliação: | RANGELINE AZEVEDO DA SILVA, UESC; GUSTAVO SOUZA, UFPE; LÍVIA SANTOS LIMA LEMOS, UFSB; UILSON VANDERLEI LOPES, CEPLAC/CEPEC; NARA GEÓRGIA RIBEIRO BRAZ PATROCÌNIO, UESC; RAFAEL MOYSES ALVES, CPATU; LUCILIA HELENA MARCELLINO, Cenargen; DIDIER CLEMENT, CEPLAC/CEPEC; FABIENNE MICHELI, UESC; KARINA PERES GRAMACHO, CEPLAC/CEPEC.
Título: | Genome size, cytogenetic data and transferability of EST-SSRs markers in wild and cultivated species of the genus Theobroma L. (Byttnerioideae, Malvaceae).
Ano de publicação: | 2017
Fonte/Imprenta: | PLoS ONE, v. 12, n. 2, 2017.
DOI: | 10.1371/journal.pone.0170799
Idioma: | Inglês
Conteúdo: | The genus Theobroma comprises several trees species native to the Amazon. Theobroma cacao L. plays a key economic role mainly in the chocolate industry. Both cultivated and wild forms are described within the genus. Variations in genome size and chromosome number have been used for prediction purposes including the frequency of interspecific hybridization or inference about evolutionary relationships. In this study, the nuclear DNA content, karyotype and genetic diversity using functional microsatellites (EST-SSR) of seven Theobroma species were characterized. The nuclear content of DNA for all analyzed Theobroma species was 1C = ~ 0.46 pg. These species presented 2n = 20 with small chromosomes and only one pair of terminal heterochromatic bands positively stained (CMA+/DAPI&#8722; bands). The small size of Theobroma ssp. genomes was equivalent to other Byttnerioideae species, suggesting that the basal lineage of Malvaceae have smaller genomes and that there was an expansion of 2C values in the more specialized family clades. A set of 20 EST-SSR primers were characterized for related species of Theobroma, in which 12 loci were polymorphic. The polymorphism information content (PIC) ranged from 0.23 to 0.65, indicating a high level of information per locus. Combined results of flow cytometry, cytogenetic data and EST-SSRs markers will contribute to better describe the species and infer about the evolutionary relationships among Theobroma species. In addition, the importance of a core collection for conservation purposes is highlighted.
Thesagro: | Theobroma Cacao.
Thesaurus Nal: | Theobroma.
Categoria do assunto: | --

URL:

http://ainfo.cnptia.embrapa.br/digital/bitstream/item/214031/1/journal.pone.0170799.pdf
http://ainfo.cnptia.embrapa.br/digital/bitstream/item/180926/1/journal.pone.0170799.pdf

Registro original: | Embrapa Amazônia Oriental (CPATU)

Exemplares:

CENARGEN - (36822-1) - UPC - AP - DD -  -  -  - DIS
CPATU - (56434-1) - UPC - AP - DD -  -  -  - DIS