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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
06/03/2017 |
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Data da última atualização: |
29/03/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SANTOS, R. C. dos; SILVA, C. R. C. da; SOARES, T. da C; CAVALCANTI, J. J. V.; LIMA, L. M. de; MARTINS, E. S.; MONNERAT, R. |
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Afiliação: |
ROSEANE CAVALCANTI DOS SANTOS, CNPA; CARLIANE REBECA COELHO da SILVA, UFRPE; TAÍZA da CUNHA SOARES, UFRPE; JOSE JAIME VASCONCELOS CAVALCANTI, CNPA; LIZIANE MARIA DE LIMA, CNPA; ÉRICA SOARES MARTINS, Cenargen; ROSE GOMES MONNERAT SOLON DE PONTES, Cenargen. |
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Título: |
Relative expression of CRy10 in GM cotton lines resistant to boll weevil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: ENCONTRO DE GENÉTICA DO NORDESTE, 21., 2016, Recife. Anais... Ribeirão Preto: SBG; Recife: UFPE: UFRPE: UPE, 2016. p. 87. |
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Idioma: |
Inglês |
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Conteúdo: |
Boll weevil (Anthonomus grandis) is a serious pest of cotton crop in several parts of world. The control is done by synthetic pesticides that increase the management costs. Embrapa coordinates an important research involving development of GM cotton resistant to boll weevil, by genetic transformation. Seven line (T0-8H) containing a Btcry 10 have been identified by feeding bioassays and ELISA, which showed reasonable toxicity against adult boll weevils. In this work, these lines were used in order to estimate the presence and relative expression of cry 10 by PCR and RT-qPCR, respectively, from young buds of 50d-plants. RT-qPCR assays were performed, by using Syber Green Rox Plus Master Mix 2X (LGC). Three endogenous cotton gene (GhACT, GhUBQ14 and GhPP2A) were used as a standard control. At first, 95°C/15 min and 40 cycles of 95°C/ 20 sec, 60°C/ 20 sec and 72°C/ 20 sec. Then, a curve of denaturation (melting curve) was performed after conclusion of amplification, at 95°C/15 sec, 60°C/15 sec, rising 2°/min until reaching 95°C. All reactions were carried out with experimental and biological replications. The threshold cycle (Ct) and PCR efficiency was estimated by Real-time PCR Miner program. The analyses were performed by using qBASEPlus program. Graphics, Cqs and Melt curve were automatically generated, based on the normalization method with a reference gene, ??Cq. Varied levels of expression were found in GM lines, too low in 8H-269 and 8H-357, mid in 8H-282 and 8H-346 and high in 8H- 336 (14x). These data agreed with previous results obtained by ELISA assays. Eleven lines derivate from 8H-336 (T1) were analyzed by PCR assays with genomic DNA, using 2 primer combinations. More than 50% showed amplicons confirming the presence of gene in selected lines. Taking in account that a reasonable level of resistance should overcoming 2 ug of protein/g tissue, we suggested that 8H-366 is the best genotype for control the cotton boll weevil. This material will be further advanced for entomological assays with larvae and adults of boll weevil. MenosBoll weevil (Anthonomus grandis) is a serious pest of cotton crop in several parts of world. The control is done by synthetic pesticides that increase the management costs. Embrapa coordinates an important research involving development of GM cotton resistant to boll weevil, by genetic transformation. Seven line (T0-8H) containing a Btcry 10 have been identified by feeding bioassays and ELISA, which showed reasonable toxicity against adult boll weevils. In this work, these lines were used in order to estimate the presence and relative expression of cry 10 by PCR and RT-qPCR, respectively, from young buds of 50d-plants. RT-qPCR assays were performed, by using Syber Green Rox Plus Master Mix 2X (LGC). Three endogenous cotton gene (GhACT, GhUBQ14 and GhPP2A) were used as a standard control. At first, 95°C/15 min and 40 cycles of 95°C/ 20 sec, 60°C/ 20 sec and 72°C/ 20 sec. Then, a curve of denaturation (melting curve) was performed after conclusion of amplification, at 95°C/15 sec, 60°C/15 sec, rising 2°/min until reaching 95°C. All reactions were carried out with experimental and biological replications. The threshold cycle (Ct) and PCR efficiency was estimated by Real-time PCR Miner program. The analyses were performed by using qBASEPlus program. Graphics, Cqs and Melt curve were automatically generated, based on the normalization method with a reference gene, ??Cq. Varied levels of expression were found in GM lines, too low in 8H-269 and 8H-357, mid in 8H-282 and 8H-346 and ... Mostrar Tudo |
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Palavras-Chave: |
Boll weevil. |
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Thesagro: |
Algodão; Anthonomus Grandis; Bicudo. |
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Thesaurus Nal: |
Cotton. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/157044/1/Relative-expression-of-Cry10.pdf
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Marc: |
LEADER 02841nam a2200241 a 4500
001 2066203
005 2023-03-29
008 2016 bl uuuu u01u1 u #d
100 1 $aSANTOS, R. C. dos
245 $aRelative expression of CRy10 in GM cotton lines resistant to boll weevil.$h[electronic resource]
260 $aIn: ENCONTRO DE GENÉTICA DO NORDESTE, 21., 2016, Recife. Anais... Ribeirão Preto: SBG; Recife: UFPE: UFRPE: UPE, 2016. p. 87.$c2016
520 $aBoll weevil (Anthonomus grandis) is a serious pest of cotton crop in several parts of world. The control is done by synthetic pesticides that increase the management costs. Embrapa coordinates an important research involving development of GM cotton resistant to boll weevil, by genetic transformation. Seven line (T0-8H) containing a Btcry 10 have been identified by feeding bioassays and ELISA, which showed reasonable toxicity against adult boll weevils. In this work, these lines were used in order to estimate the presence and relative expression of cry 10 by PCR and RT-qPCR, respectively, from young buds of 50d-plants. RT-qPCR assays were performed, by using Syber Green Rox Plus Master Mix 2X (LGC). Three endogenous cotton gene (GhACT, GhUBQ14 and GhPP2A) were used as a standard control. At first, 95°C/15 min and 40 cycles of 95°C/ 20 sec, 60°C/ 20 sec and 72°C/ 20 sec. Then, a curve of denaturation (melting curve) was performed after conclusion of amplification, at 95°C/15 sec, 60°C/15 sec, rising 2°/min until reaching 95°C. All reactions were carried out with experimental and biological replications. The threshold cycle (Ct) and PCR efficiency was estimated by Real-time PCR Miner program. The analyses were performed by using qBASEPlus program. Graphics, Cqs and Melt curve were automatically generated, based on the normalization method with a reference gene, ??Cq. Varied levels of expression were found in GM lines, too low in 8H-269 and 8H-357, mid in 8H-282 and 8H-346 and high in 8H- 336 (14x). These data agreed with previous results obtained by ELISA assays. Eleven lines derivate from 8H-336 (T1) were analyzed by PCR assays with genomic DNA, using 2 primer combinations. More than 50% showed amplicons confirming the presence of gene in selected lines. Taking in account that a reasonable level of resistance should overcoming 2 ug of protein/g tissue, we suggested that 8H-366 is the best genotype for control the cotton boll weevil. This material will be further advanced for entomological assays with larvae and adults of boll weevil.
650 $aCotton
650 $aAlgodão
650 $aAnthonomus Grandis
650 $aBicudo
653 $aBoll weevil
700 1 $aSILVA, C. R. C. da
700 1 $aSOARES, T. da C
700 1 $aCAVALCANTI, J. J. V.
700 1 $aLIMA, L. M. de
700 1 $aMARTINS, E. S.
700 1 $aMONNERAT, R.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
12/01/2012 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
SOUSA, M. M. de; CHAVES, S. C.; NASCIMENTO, M. T. dos S. C. do; SANTOS, C. M. dos; CHAGAS, A. C. de S.; VIEIRA, L. da S.; CAVALCANTE, A. C. R. |
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Afiliação: |
Maximiana Mesquita de Sousa, Graduanda, Universidade Estadual Vale do Acaraú – UVA - Sobral, CE; Sueline Cavalcante Chaves, Graduanda, UVA - Sobral, CE; Maria Thayana dos Santos Canuto do Nascimento, Graduanda, UVA - Sobral, CE; Claudine Morais dos Santos, Pós-graduanda, UVA - Sobral, CE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE; LUIZ DA SILVA VIEIRA, CNPC; ANTONIO CEZAR ROCHA CAVALCANTE, CNPC. |
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Título: |
Eficácia in vitro dos óleos essenciais das plantas Lippia sidóides, Cymbopogon shoenathus, Cymbopogon martinii e Eucalyptus stageiriana no desenvolvimento larvar de nematóides gastrintestinais de caprinos. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ZOOTECNIA, 21., 2011, Maceió. Inovações tecnológicas e mercado consumidor: anais. Maceió: Associação Brasileira de Zootecnistas, 2011. 3 f. 1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
As infecções causadas por nematóides gastrintestinais, causam prejuízos econômicos constituindo-se uma das maiores limitações à criação de caprinos principalmente aos criados de forma extensiva, onde a maioria dos produtores não contam com orientação e assistência técnica. A fitoterapia, é uma alternativa de controle que busca minimizar o uso de anti-helminticos, diminuindo o desenvolvimento de populações resistentes, prolongando a vida útil dos produtos químicos disponíveis. O presente trabalho teve por objetivo avaliar a eficácia in vitro dos óleos essenciais das plantas Lippia sidoides, Cymbopogon shoenathus, Cymbopogon martinii, e Eucalyptus stageiriana no desenvolvimento larvar de nematóides gastrintestinais de caprinos. Os óleos essenciais foram avaliados nas seguintes concentrações 5%, 2,5%, 1,25%, 0,625% e 0,312%,com um controle positivo contendo Tween 80 (3%) e um controle negativo composto por água e ovos do parasito. O percentual de inibição obtido neste experimento foi de 100% em todas as concentrações, diferindo apenas nos grupos controle. Os resultados sugerem a possibilidade da utilização destas plantas no controle de nematóídes gastrintestinais. Abstract: Gastrintestinal nematode infections are responsible for high economic losses in goat production systems, especially the extensive systems where farmers have no access to technical support and knowledge. The ever increasing problem of parasite resistance against traditional anthelmintic drugs has, among other factors, generated a high demand for alternative methods for parasite control such as phyitotherapy. In this study we evaluated the efficacy of several plant extracts (Lippia sidoides, Cymbopogon shoenathus, Cymbopogon martinii, e Eucalyptus stageiriana) as inhibitors in larval development assays against caprine gastrintestinal nematodes. Plant extracts were tested at different concentrations (5%, 2.5%, 1.25%, 0.625% and 0.312%) using 3% Tween 80 and parasite eggs as positive and negative controls respectively. All tested concentrations showed 100% efficacy and these results suggest that these extracts are strong candidates for the control of gastrintestinal parasites. MenosAs infecções causadas por nematóides gastrintestinais, causam prejuízos econômicos constituindo-se uma das maiores limitações à criação de caprinos principalmente aos criados de forma extensiva, onde a maioria dos produtores não contam com orientação e assistência técnica. A fitoterapia, é uma alternativa de controle que busca minimizar o uso de anti-helminticos, diminuindo o desenvolvimento de populações resistentes, prolongando a vida útil dos produtos químicos disponíveis. O presente trabalho teve por objetivo avaliar a eficácia in vitro dos óleos essenciais das plantas Lippia sidoides, Cymbopogon shoenathus, Cymbopogon martinii, e Eucalyptus stageiriana no desenvolvimento larvar de nematóides gastrintestinais de caprinos. Os óleos essenciais foram avaliados nas seguintes concentrações 5%, 2,5%, 1,25%, 0,625% e 0,312%,com um controle positivo contendo Tween 80 (3%) e um controle negativo composto por água e ovos do parasito. O percentual de inibição obtido neste experimento foi de 100% em todas as concentrações, diferindo apenas nos grupos controle. Os resultados sugerem a possibilidade da utilização destas plantas no controle de nematóídes gastrintestinais. Abstract: Gastrintestinal nematode infections are responsible for high economic losses in goat production systems, especially the extensive systems where farmers have no access to technical support and knowledge. The ever increasing problem of parasite resistance against traditional anthelmintic drugs has, among other... Mostrar Tudo |
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Palavras-Chave: |
Controle; Cymbopogon shoenathus; Eucalyptus stageiriana; Nematódeo gastrintestinal. |
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Thesagro: |
Caprino; Fitoterapia; Lippia sidoides; Óleo essencial. |
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Thesaurus NAL: |
Cymbopogon martinii. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/52131/1/AAC-Eficacia-in-vitro.pdf
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Marc: |
LEADER 03354nam a2200289 a 4500
001 1912518
005 2019-09-23
008 2011 bl uuuu u00u1 u #d
100 1 $aSOUSA, M. M. de
245 $aEficácia in vitro dos óleos essenciais das plantas Lippia sidóides, Cymbopogon shoenathus, Cymbopogon martinii e Eucalyptus stageiriana no desenvolvimento larvar de nematóides gastrintestinais de caprinos.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE ZOOTECNIA, 21., 2011, Maceió. Inovações tecnológicas e mercado consumidor: anais. Maceió: Associação Brasileira de Zootecnistas, 2011. 3 f. 1 CD-ROM.$c2011
520 $aAs infecções causadas por nematóides gastrintestinais, causam prejuízos econômicos constituindo-se uma das maiores limitações à criação de caprinos principalmente aos criados de forma extensiva, onde a maioria dos produtores não contam com orientação e assistência técnica. A fitoterapia, é uma alternativa de controle que busca minimizar o uso de anti-helminticos, diminuindo o desenvolvimento de populações resistentes, prolongando a vida útil dos produtos químicos disponíveis. O presente trabalho teve por objetivo avaliar a eficácia in vitro dos óleos essenciais das plantas Lippia sidoides, Cymbopogon shoenathus, Cymbopogon martinii, e Eucalyptus stageiriana no desenvolvimento larvar de nematóides gastrintestinais de caprinos. Os óleos essenciais foram avaliados nas seguintes concentrações 5%, 2,5%, 1,25%, 0,625% e 0,312%,com um controle positivo contendo Tween 80 (3%) e um controle negativo composto por água e ovos do parasito. O percentual de inibição obtido neste experimento foi de 100% em todas as concentrações, diferindo apenas nos grupos controle. Os resultados sugerem a possibilidade da utilização destas plantas no controle de nematóídes gastrintestinais. Abstract: Gastrintestinal nematode infections are responsible for high economic losses in goat production systems, especially the extensive systems where farmers have no access to technical support and knowledge. The ever increasing problem of parasite resistance against traditional anthelmintic drugs has, among other factors, generated a high demand for alternative methods for parasite control such as phyitotherapy. In this study we evaluated the efficacy of several plant extracts (Lippia sidoides, Cymbopogon shoenathus, Cymbopogon martinii, e Eucalyptus stageiriana) as inhibitors in larval development assays against caprine gastrintestinal nematodes. Plant extracts were tested at different concentrations (5%, 2.5%, 1.25%, 0.625% and 0.312%) using 3% Tween 80 and parasite eggs as positive and negative controls respectively. All tested concentrations showed 100% efficacy and these results suggest that these extracts are strong candidates for the control of gastrintestinal parasites.
650 $aCymbopogon martinii
650 $aCaprino
650 $aFitoterapia
650 $aLippia sidoides
650 $aÓleo essencial
653 $aControle
653 $aCymbopogon shoenathus
653 $aEucalyptus stageiriana
653 $aNematódeo gastrintestinal
700 1 $aCHAVES, S. C.
700 1 $aNASCIMENTO, M. T. dos S. C. do
700 1 $aSANTOS, C. M. dos
700 1 $aCHAGAS, A. C. de S.
700 1 $aVIEIRA, L. da S.
700 1 $aCAVALCANTE, A. C. R.
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Embrapa Caprinos e Ovinos (CNPC) |
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