Data/hora: 10/06/2024 18:42:09

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	07/02/2017	
Data da última atualização: 	30/01/2023	
Autoria: 	GUIMARÃES-CESTARO, L.; SERRÃO, J. E.; MESSAGE, D.; MARTINS, M. F.; TEIXEIRA, É. W.	
Afiliação: 	Lubiane Guimarães-Cestaro, UFV; José Eduardo Serrão, UFV; Dejair Message, UFERSA; MARTA FONSECA MARTINS, CNPGL; Érica Weinstein Teixeira, APTA-SAA.	
Título: 	Simultaneous detection of Nosema spp., Ascosphaera apis and Paenibacillus larvae in honey bee products.	
Ano de publicação: 	2016	
Fonte/Imprenta: 	Journal of Hymenoptera Research, n. 49, p. 43-50, 2016.	
Idioma: 	Inglês	
Conteúdo: 	Abstract Honey bees are responsible for pollinating many native and cultivated plant species. These insects can be affected by many pathogens, including fungi and bacteria, both of which can form spores that are easily dispersed within the colony by means of the stored products, among other routes. The objective of this study was to develop a method to detect spores of the honey bee pathogens Nosema apis, Nosema ceranae, Ascosphaera apis and Paenibacillus larvae in samples of honey, bee pollen and royal jelly. The method was standardized for each product individually, and then analyzed by monoplex and multiplex PCR, which showed the same detection thresholds: 1.25 spores/mL of honey for N. ceranae; 7.5 spores/mL of honey for A. apis; and 0.4 spore/mL of honey for P. larvae, respectively. The standardized technique was effective and rapid for the detection of these pathogens in bee products and can be used for the establishment of official methods of sanitary control of bee products, considering the growing national and international trade of these products and the movement or migration of colonies between regions.	
Palavras-Chave: 	Bee pathogens; Detection of spores.	
Thesagro: 	Apis Mellifera.	
Thesaurus Nal: 	honey; pollen; royal jelly.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/154930/1/Cnpgl-2016-JHymenopRes-Simultaneous.pdf	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (23124-1) - UPC - AP - DD -  -  -  - DIS