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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
23/09/2019 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
GREGORINE, L. F.; MONCLARO, A. V.; SILVA, C. de O. G.; RODRIGUES, K. B.; PEIXOTO, J. S. G.; ABDELNUR, P. V.; FAVARO, L. C. de L. |
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Afiliação: |
Lucas F. Gregorine; Antonielle V. Monclaro; Caio de Oliveira Gorgulho Silva, Consultor da Embrapa Agroenergia; Kelly B. Rodrigues; Jéssica S. G. Peixoto, UnB; PATRICIA VERARDI ABDELNUR, CNPAE; LEIA CECILIA DE LIMA FAVARO, CNPAE. |
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Título: |
Heterologous expression of a new lytic polysaccharide monooxygenase from Hahella ganghwensis and their functional characterization. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 22.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 13., 2019, Uberlandia, MG. [Anais ...]. São Paulo: Associação Brasileira de Engenharia Química, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
The powerful class of oxidative enzymes, lytic polysaccharide monooxygenases (LPMOs) - also named Auxiliary Activity (AA) - are able to oxidize recalcitrant polysaccharides on lignocellulosic biomass. In this work, we successfully expressed three catalytic domains from bacterial LPMOs in the yeast Komagataella phaffii: domain MdAA10.1-SD (from Moritella dasanensis), domain VmAA10.2-SD (from Verrucosispora maris), and domain HgAA10.1-SD (from Hahella ganghwensis). Heterologous expression was analyzed by SDS-PAGE, Western-Blot, and Dot-Blot, while functional activity of these proteins was investigated by a combination of mass spectrometric and chromatographic methods. The recombinant LPMO catalytic domain HgAA10.1-SD from H. ganghwensis, a C1-oxidizer, was able to promote an oxidative cleavage of phosphoric-acid swollen cellulose (PASC) substrate in the presence of ascorbic acid as an electron donor, showing its potential for cellulose depolymerization. |
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Palavras-Chave: |
Chromatographic; Spectrometric. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/202240/1/107193-786998-field-submission-fulltext-file1.pdf
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Marc: |
LEADER 01787nam a2200205 a 4500
001 2112393
005 2019-09-23
008 2019 bl uuuu u00u1 u #d
100 1 $aGREGORINE, L. F.
245 $aHeterologous expression of a new lytic polysaccharide monooxygenase from Hahella ganghwensis and their functional characterization.$h[electronic resource]
260 $aIn: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 22.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 13., 2019, Uberlandia, MG. [Anais ...]. São Paulo: Associação Brasileira de Engenharia Química$c2019
520 $aThe powerful class of oxidative enzymes, lytic polysaccharide monooxygenases (LPMOs) - also named Auxiliary Activity (AA) - are able to oxidize recalcitrant polysaccharides on lignocellulosic biomass. In this work, we successfully expressed three catalytic domains from bacterial LPMOs in the yeast Komagataella phaffii: domain MdAA10.1-SD (from Moritella dasanensis), domain VmAA10.2-SD (from Verrucosispora maris), and domain HgAA10.1-SD (from Hahella ganghwensis). Heterologous expression was analyzed by SDS-PAGE, Western-Blot, and Dot-Blot, while functional activity of these proteins was investigated by a combination of mass spectrometric and chromatographic methods. The recombinant LPMO catalytic domain HgAA10.1-SD from H. ganghwensis, a C1-oxidizer, was able to promote an oxidative cleavage of phosphoric-acid swollen cellulose (PASC) substrate in the presence of ascorbic acid as an electron donor, showing its potential for cellulose depolymerization.
653 $aChromatographic
653 $aSpectrometric
700 1 $aMONCLARO, A. V.
700 1 $aSILVA, C. de O. G.
700 1 $aRODRIGUES, K. B.
700 1 $aPEIXOTO, J. S. G.
700 1 $aABDELNUR, P. V.
700 1 $aFAVARO, L. C. de L.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
29/11/2017 |
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Data da última atualização: |
07/02/2024 |
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Tipo da produção científica: |
Documentos |
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Autoria: |
SILVA, M. V. G. B.; MARTINS, M. F.; CEMBRANELLI, M. DE A. R.; PANETTO, J. C. do C.; PAIVA, L. DE C.; GONÇALVES, G. S.; MACHADO, M. A.; ALVES, B. R. C. |
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Afiliação: |
MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; MARTA FONSECA MARTINS, CNPGL; Marcello de Aguiar Rodrigues Cembranelli, ABCG; JOAO CLAUDIO DO CARMO PANETTO, CNPGL; Leandro de Carvalho Paiva, ABCG; Gustavo Souza Gonçalves, ABCG; MARCO ANTONIO MACHADO, CNPGL; BRUNA RIOS COELHO ALVES, CNPGL. |
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Título: |
Girolando breed genetic improvement program - Genetic evaluation of cows - June/2017. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Juiz de Fora: Embrapa Gado de Leite, 2017. |
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Páginas: |
33 p. |
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Série: |
(Embrapa Gado de Leite. Documentos, 208). |
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ISSN: |
1516-7453 |
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Idioma: |
Inglês |
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Conteúdo: |
The Girolando breed progeny test was established in 1997, as a result of the partnership between Girolando and Embrapa Dairy Cattle. In 2007, the Programa de Melhoramento Genético da Raça Girolando - PMGG (Genetic Improvement Program of the Girolando Breed) was implemented. This Sire Summary/Progeny Test Results document has innovative tools and resources, for breeders and/or other professionals. Those include information regarding molecular markers, the Girolando Linear Evaluation System (SALG) and the updated genetic evaluation of age at first calving. The methods for analysis were modified for this current evaluation and are detailed further in this document. |
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Palavras-Chave: |
Gado de leite; Girolando breed; Girolando cows; Melhoramento genético; Raça girolando. |
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Thesaurus NAL: |
animal breeding; dairy cattle. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/167773/1/DOC-208-Sumario-de-Vacas-ingles-2017.pdf
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Marc: |
LEADER 01598nam a2200313 a 4500
001 2081019
005 2024-02-07
008 2017 bl uuuu u0uu1 u #d
022 $a1516-7453
100 1 $aSILVA, M. V. G. B.
245 $aGirolando breed genetic improvement program - Genetic evaluation of cows - June/2017.$h[electronic resource]
260 $aJuiz de Fora: Embrapa Gado de Leite$c2017
300 $a33 p.
490 $a(Embrapa Gado de Leite. Documentos, 208).
520 $aThe Girolando breed progeny test was established in 1997, as a result of the partnership between Girolando and Embrapa Dairy Cattle. In 2007, the Programa de Melhoramento Genético da Raça Girolando - PMGG (Genetic Improvement Program of the Girolando Breed) was implemented. This Sire Summary/Progeny Test Results document has innovative tools and resources, for breeders and/or other professionals. Those include information regarding molecular markers, the Girolando Linear Evaluation System (SALG) and the updated genetic evaluation of age at first calving. The methods for analysis were modified for this current evaluation and are detailed further in this document.
650 $aanimal breeding
650 $adairy cattle
653 $aGado de leite
653 $aGirolando breed
653 $aGirolando cows
653 $aMelhoramento genético
653 $aRaça girolando
700 1 $aMARTINS, M. F.
700 1 $aCEMBRANELLI, M. DE A. R.
700 1 $aPANETTO, J. C. do C.
700 1 $aPAIVA, L. DE C.
700 1 $aGONÇALVES, G. S.
700 1 $aMACHADO, M. A.
700 1 $aALVES, B. R. C.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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