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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
19/12/2018 |
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Data da última atualização: |
29/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, P. R. A. da; VIDAL, M. S.; SOARES, C. de P.; POLESE, V.; TADRA-SFEIR, M. Z.; SOUZA, E. M. de; ARAUJO, J. L. S. de; BALDANI, J. I. |
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Afiliação: |
PAULA RENATA ALVES DA SILVA, BOLSISTA EMBRAPA AGROBIOLOGIA; MARCIA SOARES VIDAL, CNPAB; CLEITON DE PAULA SOARES, UFRJ; VALÉRIA POLESE, UFRJ; MICHELLE ZIBETTI TADRA-SFEIR,, UFRJ; EMANUEL MALTEMPI DE SOUZA, UFRJ; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; JOSE IVO BALDANI, CNPAB. |
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Título: |
Sugarcane apoplast fluid modulates the global transcriptional profile of the diazotrophic bacteria Paraburkholderia tropica strain Ppe8. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
PLOS ONE, v. 13, n. 12, e0207863, 2018. |
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ISSN: |
1932-6203 |
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Idioma: |
Português |
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Conteúdo: |
The stalk apoplast fluid of sugarcane contains different sugars, organic acids and amino acids that may supply the demand for carbohydrates by endophytic bacteria including diazotrophs P. tropica (syn. B. tropica) strain Ppe8, isolated from sugarcane, is part of the bacterial consortium recommended as inoculant to sugarcane. However, little information has been accumulated regarding this plant-bacterium interaction considering that it colonizes internal sugarcane tissues. Here, we made use of the RNA-Seq transcriptomic analysis to study the influence of sugarcane stalk apoplast fluid on Ppe8 gene expression. The bacterium was grown in JMV liquid medium (100 ml), divided equally and then supplemented with 50 ml of fresh JMV medium or 50 ml of apoplast fluid extracted from sugarcane variety RB867515. Total RNA was extracted 2 hours later, the rRNAs were depleted and mRNAs used to construct libraries to sequence the fragments using Ion Torrent technology. The mapping and statistical analysis were carried out with CLC Genomics Workbench software. The RNA-seq data was validated by RT-qPCR using the reference genes fliP1, paaF, and groL. The data analysis showed that 544 genes were repressed and 153 genes were induced in the presence of apoplast fluid. Genes that induce plant defense responses, genes related to chemotaxis and movements were repressed in the presence of apoplast fluid, indicating that strain Ppe8 recognizes the apoplast fluid as a plant component. The expression of genes involved in bacterial metabolism was regulated (up and down), suggesting that the metabolism of strain Ppe8 is modulated by the apoplast fluid. These results suggest that Ppe8 alters its gene expression pattern in the presence of apoplast fluid mainly in order to use compounds present in the fluid as well as to avoid the induction of plant defense mechanisms. This is a pioneer study showing the role played by the sugarcane apoplast fluid on the global modulation of genes in P. tropica strain Ppe8. MenosThe stalk apoplast fluid of sugarcane contains different sugars, organic acids and amino acids that may supply the demand for carbohydrates by endophytic bacteria including diazotrophs P. tropica (syn. B. tropica) strain Ppe8, isolated from sugarcane, is part of the bacterial consortium recommended as inoculant to sugarcane. However, little information has been accumulated regarding this plant-bacterium interaction considering that it colonizes internal sugarcane tissues. Here, we made use of the RNA-Seq transcriptomic analysis to study the influence of sugarcane stalk apoplast fluid on Ppe8 gene expression. The bacterium was grown in JMV liquid medium (100 ml), divided equally and then supplemented with 50 ml of fresh JMV medium or 50 ml of apoplast fluid extracted from sugarcane variety RB867515. Total RNA was extracted 2 hours later, the rRNAs were depleted and mRNAs used to construct libraries to sequence the fragments using Ion Torrent technology. The mapping and statistical analysis were carried out with CLC Genomics Workbench software. The RNA-seq data was validated by RT-qPCR using the reference genes fliP1, paaF, and groL. The data analysis showed that 544 genes were repressed and 153 genes were induced in the presence of apoplast fluid. Genes that induce plant defense responses, genes related to chemotaxis and movements were repressed in the presence of apoplast fluid, indicating that strain Ppe8 recognizes the apoplast fluid as a plant component. The expression of... Mostrar Tudo |
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Palavras-Chave: |
Bacterial growth; RNA Sequence; Sugarcane variety RB867515; Transcriptomic profile. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02828naa a2200265 a 4500
001 2102134
005 2019-04-29
008 2018 bl uuuu u00u1 u #d
022 $a1932-6203
100 1 $aSILVA, P. R. A. da
245 $aSugarcane apoplast fluid modulates the global transcriptional profile of the diazotrophic bacteria Paraburkholderia tropica strain Ppe8.$h[electronic resource]
260 $c2018
520 $aThe stalk apoplast fluid of sugarcane contains different sugars, organic acids and amino acids that may supply the demand for carbohydrates by endophytic bacteria including diazotrophs P. tropica (syn. B. tropica) strain Ppe8, isolated from sugarcane, is part of the bacterial consortium recommended as inoculant to sugarcane. However, little information has been accumulated regarding this plant-bacterium interaction considering that it colonizes internal sugarcane tissues. Here, we made use of the RNA-Seq transcriptomic analysis to study the influence of sugarcane stalk apoplast fluid on Ppe8 gene expression. The bacterium was grown in JMV liquid medium (100 ml), divided equally and then supplemented with 50 ml of fresh JMV medium or 50 ml of apoplast fluid extracted from sugarcane variety RB867515. Total RNA was extracted 2 hours later, the rRNAs were depleted and mRNAs used to construct libraries to sequence the fragments using Ion Torrent technology. The mapping and statistical analysis were carried out with CLC Genomics Workbench software. The RNA-seq data was validated by RT-qPCR using the reference genes fliP1, paaF, and groL. The data analysis showed that 544 genes were repressed and 153 genes were induced in the presence of apoplast fluid. Genes that induce plant defense responses, genes related to chemotaxis and movements were repressed in the presence of apoplast fluid, indicating that strain Ppe8 recognizes the apoplast fluid as a plant component. The expression of genes involved in bacterial metabolism was regulated (up and down), suggesting that the metabolism of strain Ppe8 is modulated by the apoplast fluid. These results suggest that Ppe8 alters its gene expression pattern in the presence of apoplast fluid mainly in order to use compounds present in the fluid as well as to avoid the induction of plant defense mechanisms. This is a pioneer study showing the role played by the sugarcane apoplast fluid on the global modulation of genes in P. tropica strain Ppe8.
653 $aBacterial growth
653 $aRNA Sequence
653 $aSugarcane variety RB867515
653 $aTranscriptomic profile
700 1 $aVIDAL, M. S.
700 1 $aSOARES, C. de P.
700 1 $aPOLESE, V.
700 1 $aTADRA-SFEIR, M. Z.
700 1 $aSOUZA, E. M. de
700 1 $aARAUJO, J. L. S. de
700 1 $aBALDANI, J. I.
773 $tPLOS ONE$gv. 13, n. 12, e0207863, 2018.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
13/08/2010 |
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Data da última atualização: |
04/05/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 4 |
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Autoria: |
CASTRO, F. da S.; PEZZOPANE, J. R. M.; PEZZOPANE, J. E. M.; CECÍLIO, R. A.; XAVIER, A. C. |
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Afiliação: |
FÁBIO DA SILVEIRA CASTRO, ENGENHEIRO AGRÔNOMO/PORTO ALEGRE; JOSE RICARDO MACEDO PEZZOPANE, CPPSE; JOSÉ EDUARDO MACEDO PEZZOPANE, PROF. UFES/ALEGRE, ES; ROBERTO AVELINO CECÍLIO, PROF. UFES, ALEGRE, ES; ALEXANDRE CÂNDIDO XAVIER, PROD. UFES/ALEGRE, ES. |
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Título: |
Zoneamento agroclimático para espécies do gênero pinus no estado do Espirito Santo. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Floresta, Curitiba, v. 40, n. 1, p. 235-250, 2010. |
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Idioma: |
Português |
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Conteúdo: |
Com o objetivo de determinar a aptidão a groclimática das espécies Pinus elliottii Engelm var. elliottii, Pinus caribaea var. hondurensis Morelet, Pinus taeda Linnaeuse Pinus oocarpa Schiedee x Schltdl no Espirito Santo (ES), foi realizado o zoneamentoa groclimatico do estado. Utilizaram-se dados meteorológicos do período de 1977 a 2006, provenientes de 96 postos pluviometricos da Agência Nacionald e Águas (ANA), sendo 80 localizados dentro do estado e 16 em estados circunvizinhos de 11 estações meteorológicas pertencentes ao INCAPER (Instituto Capixaba de Pesquisa e Extensão Rural) e de três do INMET (Instituto Nacional de Meteorologia). as elementos climáticos para detenninar a aptidão foram a temperatura do ar e a deficiência hídrica anual para a capacidade de água disponivel de 300 mm. De acordo com os resultados O estado apresenta áreas com aptidão climática para todas as especies de Pinus, sendo que a espécie Pinus caribaea var. hondurensis apresenta a maior área de aptidão quando comparada com as demais, devido às suas exigências climáticas serem mais restritas. |
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Palavras-Chave: |
Zoneamento agroclimático. |
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Thesagro: |
Balanço Hídrico; Sistema de Informação Geográfica. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/25025/1/PROCIJRMP2010.00049.pdf
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Marc: |
LEADER 01736naa a2200205 a 4500
001 1859997
005 2012-05-04
008 2010 bl uuuu u00u1 u #d
100 1 $aCASTRO, F. da S.
245 $aZoneamento agroclimático para espécies do gênero pinus no estado do Espirito Santo.
260 $c2010
520 $aCom o objetivo de determinar a aptidão a groclimática das espécies Pinus elliottii Engelm var. elliottii, Pinus caribaea var. hondurensis Morelet, Pinus taeda Linnaeuse Pinus oocarpa Schiedee x Schltdl no Espirito Santo (ES), foi realizado o zoneamentoa groclimatico do estado. Utilizaram-se dados meteorológicos do período de 1977 a 2006, provenientes de 96 postos pluviometricos da Agência Nacionald e Águas (ANA), sendo 80 localizados dentro do estado e 16 em estados circunvizinhos de 11 estações meteorológicas pertencentes ao INCAPER (Instituto Capixaba de Pesquisa e Extensão Rural) e de três do INMET (Instituto Nacional de Meteorologia). as elementos climáticos para detenninar a aptidão foram a temperatura do ar e a deficiência hídrica anual para a capacidade de água disponivel de 300 mm. De acordo com os resultados O estado apresenta áreas com aptidão climática para todas as especies de Pinus, sendo que a espécie Pinus caribaea var. hondurensis apresenta a maior área de aptidão quando comparada com as demais, devido às suas exigências climáticas serem mais restritas.
650 $aBalanço Hídrico
650 $aSistema de Informação Geográfica
653 $aZoneamento agroclimático
700 1 $aPEZZOPANE, J. R. M.
700 1 $aPEZZOPANE, J. E. M.
700 1 $aCECÍLIO, R. A.
700 1 $aXAVIER, A. C.
773 $tFloresta, Curitiba$gv. 40, n. 1, p. 235-250, 2010.
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Embrapa Pecuária Sudeste (CPPSE) |
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