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| 2. |  | OSPINA, B.; CEBALLOS, H. La yuca en el tercer milenio: sistemas modernos de producción, processamiento, utilización y comercialización. Cali: CIAT, 2002. 586 p. (Publicación CIAT, n.327). Suplementado por: ALVAREZ, E.; BELLOTTI, A.; CALVER, L.; CADAVID, L. F.; PINEDA, B.; LLANO, G.; CUERVO, M. Guía práctica para el manejo de las enfermedades, las plagas y las deficiencias nutricionales de la yuca. Cali: CIAT, 2002. 120 p.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | URREA, R.; CEBALLOS, H.; PANDEY, S.; BAHIA, A. F. C.; LEON, L. A.; FENALCE, A. A. Greenhouse screening technique for soil acidity tolerance in maize (ZEA MAYS L.). In: INTERNATIONAL SYMPOSIUM ON PLANT-SOIL INTERACTIONS AT LOW pH, 4., 1996, Belo Horizonte. Abstracts/Programs. Sete Lagoas: EMBRAPA-CNPMS, 1996. p.81.| Biblioteca(s): Embrapa Cerrados. |
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| 8. | | URREA, R.; CEBALLOS, H.; PANDEY, S.; BAHIA, A. F. C.; LEON, L. A.; FENALCE, A. A. Greenhouse screening technique for soil acidity tolerance in maize (Zea mays L.) In: INTERNATIONAL SYMPOSIUM ON PLANT SOIL INTERACTIONS AT LOW pH, 4., 1996, Belo Horizonte. Abstracts... Sete Lagoas: EMBRAPA-CNPMS, 1996. p. 47.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 9. | | FUKUDA, W.; OLIVEIRA, L.; PEREIRA, M.; CEBALLOS, H.; CARVALHO, J.; NUTTI, M.; DITA, M. Breeding cassava for enhancement of carotenoid in the biofortification program in Brazil. In: INTERNATIONAL MEETING OF THE MICRONUTRIENT FORUM, 2., 2009, Beijing, China. Micronutrients, health and development: evidence-based programs: program/abstracts. Washington, DC: USAID: Academy for Educational Development, 2009. p. 106.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 10. | | FUKUDA, W.; SANTOS, V.; OLIVEIRA, L.; PEREIRA, M.; CEBALLOS, H.; NUTTI, M.; CARVALHO, J.; DITA, M. Breeding cassava for enhancement of carotenoid, iron and zinc contents. In: SCIENTIFIC MEETING OF THE GLOBAL CASSAVA PARTNERSHIP, 1., 2008, Ghent, Belgium. Cassava: meeting the challenges of the new millennium. Ghent, Belgium: Institute of Plant Biotechnology for Developing Countries, 2008. p. 106. S7-9.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 11. | | FUKUDA, W.; SANTOS, V.; OLIVEIRA, L.; PEREIRA, M.; CEBALLOS, H.; NUTTI, M.; CARVALHO, J.; DITA, MIGUEL. Breeding cassava for enhancement of carotenoid, iron and zinc contents. In: SCIENTIFIC MEETING OF THE GLOBAL CASSAVA PARTNERSHIP, 1., 2008, Ghent. Cassava: meeting the challenges of the new millennium.Ghent:: IPBO, 2008. p. 106. S7-9.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 12. | | FUKUDA, W.; OLIVEIRA, L.; SANTOS, V.; CEBALLOS, H.; NUTTI, M.; CARVALHO, J. L. Development of cassava cultivars with high iron and zinc contents roots. In: SCIENTIFIC MEETING OF THE GLOBAL CASSAVA PARTNERSHIP, 1., 2008, Ghent. Cassava: meeting the challenges of the new millennium. Ghent:: IPBO, 2008. p. 111. SP07-09.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 13. | | FUKUDA, W.; OLIVEIRA, L.; SANTOS, V.; CEBALLOS, H.; NUTTI, M.; CARVALHO, J. L. Development of cassava cultivars with high iron and zinc contents in roots. In: SCIENTIFIC MEETING OF THE GLOBAL CASSAVA PARTNERSHIP, 1., 2008, Ghent, Belgium. Cassava: meeting the challenges of the new millennium. Ghent, Belgium: Institute of Plant Biotechnology for Developing Countries, 2008. p. 111. SP07-09.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 14. | | BORRERO, J. C.; PANDEY, S.; CEBALLOS, H.; MAGNAVACA, R.; BAHIA FILHO, A. F. C. Genetic variances for tolerance to soil acidity in a tropical maize population. Maydica, Bergamo, v. 40, n. 3, p. 283-288, 1995.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 18. | | SALAZAR, F. S.; PANDEY, S.; NARRO, L.; PEREZ, J. C.; CEBALLOS, H.; PARENTONI, S. N.; BAHIA FILHO, A. F. C. Diallel analysis of acid-soil tolerant and intolerant tropical maize populations. Crop Science, Madison, v. 37, n. 5, p. 1457-1462, 1997.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 19. | | FUKUDA, W. M. G.; OLIVEIRA, L. A. de; SANTOS, V. da S.; NUTTI, M.; CARVALHO, J. L. V. de; CEBALLOS, H. Mejoramiento de yuca para el contenido de carotenos, hierro y zinc en las raíces. In: REUNIÓN ANUAL DEL PROGRAMA COOPERATIVO CENTROAMERICANO PARA EL MEJORAMIENTO DE CULTIVOS Y ANIMALES, 54., 2008, San José, Costa Rica. Resúmenes. [S.l.: s.n.], 2008. p. 103.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 20. | | NUTTI, M.; BEEBE, S.; GRENIER, C.; VICENTE, F. SAN; GRUNEBERG, W.; CEBALLOS, H.; CARVALHO, J. V. de; TOHME, J.; GONZÁLEZ, C.; TALSMA, E. Mejorando agricultura, mejorando nutrición. In: REUNIÓN ANUAL PROGRAMA COOPERATIVO CENTROAMERICANO PARA EL MEJORAMIENTO DE CULTIVOS Y ANIMALES, 61., 2016, Costa Rica. Trabajos. Costa Rica: Instituto de Ciencia y Tecnología Agrícola, 2016. 1 p. PCCMCA. LEG 47.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| Registros recuperados : 35 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
11/05/2020 |
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Data da última atualização: |
15/07/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CARMO, C. D. do; SOUSA, M. B. e; SILVA, P. P. dos S.; OLIVEIRA, G. A. F.; CEBALLOS, H.; OLIVEIRA, E. J. de. |
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Afiliação: |
CÁTIA DIAS DO CARMO; MASSAINE BANDEIRA E SOUSA; PRISCILA PATRÍCIA DOS SANTOS SILVA; GILMARA ALVARENGA FACHARDO OLIVEIRA; HERNÁN CEBALLOS; EDER JORGE DE OLIVEIRA, CNPMF. |
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Título: |
Identification and validation of mutation points associated with waxy phenotype in cassava. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
BMC Plant Biology, v. 20, p.164, 2020. |
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ISSN: |
1471-2229 |
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Idioma: |
Inglês |
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Conteúdo: |
BACKGROUND: The granule-bound starch synthase I (GBSSI) enzyme is responsible for the synthesis of amylose, and therefore, its absence results in individuals with a waxy starch phenotype in various amylaceous crops. The validation of mutation points previously associated with the waxy starch phenotype in cassava, as well as the identification of alternative mutant alleles in the GBSSI gene, can allow the development of molecular-assisted selection to introgress the waxy starch mutation into cassava breeding populations. RESULTS: A waxy cassava allele has been identified previously, associated with several SNPs. A particular SNP (intron 11) was used to develop SNAP markers for screening heterozygote types in cassava germplasm. Although the molecular segregation corresponds to the expected segregation at 3:1 ratio (dominant gene for the presence of amylose), the homozygotes containing the SNP associated with the waxy mutation did not show waxy phenotypes. To identify more markers, we sequenced the GBSS gene from 89 genotypes, including some that were segregated from a cross with a line carrying the known waxy allele. As a result, 17 mutations in the GBSSI gene were identified, in which only the deletion in exon 6 (MeWxEx6-del-C) was correlated with the waxy phenotype. The evaluation of mutation points by discriminant analysis of principal component analysis (DAPC) also did not completely discriminate the waxy individuals. Therefore, we developed Kompetitive Allele Specific PCR (KASP) markers that allowed discrimination between WX and wx alleles. The results demonstrated the non-existence of heterozygous individuals of the MeWxEx6-del-C deletion in the analyzed germplasm. Therefore, the deletion MeWxEx6-del-C should not be used for assisted selection in genetic backgrounds different from the original source of waxy starch. Also, the alternative SNPs identified in this study were not associated with the waxy phenotype when compared to a panel of accessions with high genetic diversity. CONCLUSION: Although the GBSSI gene can exhibit several mutations in cassava, only the deletion in exon 6 (MeWxEx6-del-C) was correlated with the waxy phenotype in the original AM206-5 source. MenosBACKGROUND: The granule-bound starch synthase I (GBSSI) enzyme is responsible for the synthesis of amylose, and therefore, its absence results in individuals with a waxy starch phenotype in various amylaceous crops. The validation of mutation points previously associated with the waxy starch phenotype in cassava, as well as the identification of alternative mutant alleles in the GBSSI gene, can allow the development of molecular-assisted selection to introgress the waxy starch mutation into cassava breeding populations. RESULTS: A waxy cassava allele has been identified previously, associated with several SNPs. A particular SNP (intron 11) was used to develop SNAP markers for screening heterozygote types in cassava germplasm. Although the molecular segregation corresponds to the expected segregation at 3:1 ratio (dominant gene for the presence of amylose), the homozygotes containing the SNP associated with the waxy mutation did not show waxy phenotypes. To identify more markers, we sequenced the GBSS gene from 89 genotypes, including some that were segregated from a cross with a line carrying the known waxy allele. As a result, 17 mutations in the GBSSI gene were identified, in which only the deletion in exon 6 (MeWxEx6-del-C) was correlated with the waxy phenotype. The evaluation of mutation points by discriminant analysis of principal component analysis (DAPC) also did not completely discriminate the waxy individuals. Therefore, we developed Kompetitive Allele Specific PCR... Mostrar Tudo |
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Thesagro: |
Mandioca. |
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Thesaurus NAL: |
Cassava. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02827naa a2200217 a 4500
001 2122191
005 2020-07-15
008 2020 bl uuuu u00u1 u #d
022 $a1471-2229
100 1 $aCARMO, C. D. do
245 $aIdentification and validation of mutation points associated with waxy phenotype in cassava.$h[electronic resource]
260 $c2020
520 $aBACKGROUND: The granule-bound starch synthase I (GBSSI) enzyme is responsible for the synthesis of amylose, and therefore, its absence results in individuals with a waxy starch phenotype in various amylaceous crops. The validation of mutation points previously associated with the waxy starch phenotype in cassava, as well as the identification of alternative mutant alleles in the GBSSI gene, can allow the development of molecular-assisted selection to introgress the waxy starch mutation into cassava breeding populations. RESULTS: A waxy cassava allele has been identified previously, associated with several SNPs. A particular SNP (intron 11) was used to develop SNAP markers for screening heterozygote types in cassava germplasm. Although the molecular segregation corresponds to the expected segregation at 3:1 ratio (dominant gene for the presence of amylose), the homozygotes containing the SNP associated with the waxy mutation did not show waxy phenotypes. To identify more markers, we sequenced the GBSS gene from 89 genotypes, including some that were segregated from a cross with a line carrying the known waxy allele. As a result, 17 mutations in the GBSSI gene were identified, in which only the deletion in exon 6 (MeWxEx6-del-C) was correlated with the waxy phenotype. The evaluation of mutation points by discriminant analysis of principal component analysis (DAPC) also did not completely discriminate the waxy individuals. Therefore, we developed Kompetitive Allele Specific PCR (KASP) markers that allowed discrimination between WX and wx alleles. The results demonstrated the non-existence of heterozygous individuals of the MeWxEx6-del-C deletion in the analyzed germplasm. Therefore, the deletion MeWxEx6-del-C should not be used for assisted selection in genetic backgrounds different from the original source of waxy starch. Also, the alternative SNPs identified in this study were not associated with the waxy phenotype when compared to a panel of accessions with high genetic diversity. CONCLUSION: Although the GBSSI gene can exhibit several mutations in cassava, only the deletion in exon 6 (MeWxEx6-del-C) was correlated with the waxy phenotype in the original AM206-5 source.
650 $aCassava
650 $aMandioca
700 1 $aSOUSA, M. B. e
700 1 $aSILVA, P. P. dos S.
700 1 $aOLIVEIRA, G. A. F.
700 1 $aCEBALLOS, H.
700 1 $aOLIVEIRA, E. J. de
773 $tBMC Plant Biology$gv. 20, p.164, 2020.
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