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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
22/03/2012 |
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Data da última atualização: |
22/03/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FONSECA JÚNIOR, A. A.; MAGALHÃES, C. G.; SALES, E. B.; D'AMBROS, R. M.; ZANELLA, J. R. C.; HEINEMANN, M. B.; LEITE, R. C.; REIS, J. K. P. dos. |
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Afiliação: |
ANTONIO AUGUSTO FONSECA JÚNIOR, LANAGRO; CRISTINA GONÇALVES MAGALHÃES, LANAGRO; ERICA BRAVO SALES, LANAGRO; REGIA MARIA D'AMBROS, CEDISA; JANICE REIS CIACCI ZANELLA, CNPSA; MARCOS BRYAN HEINEMANN, UFMG; ROMULO CERQUEIRA LEITE, UFMG; JENNER KARLISSON PIMENTA DOS REIS, UFMG. |
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Título: |
Genotyping of the Pseudorabies vírus by multiplex PCR followed by restriction enzyme analysis. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
ISRN Microbiology, v. 2011. Doi:10.5402/2011/458294. |
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Idioma: |
Inglês |
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Notas: |
Projeto/Plano de Ação: 03.09.00.046. |
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Conteúdo: |
Suid herpesvirus 1 (SuHV-1) is the causative agent of Aujeszky?s disease. The infectious agent has only one serotype, but it was classified by restriction enzyme analysis of the whole genome into four genotypes, named I to IV. The aim of this study was to standardize a rapid method for genotyping SuHV-1 without virus isolation, using a multiplex-PCR followed by enzymatic restriction analysis. The complete genome of the virus was analyzed in silico to determine the restriction sites for the enzyme BamHI. Primers were designed to flank sites with emphasis on certain points of differentiation of genotypes. The standard PCRs were able to detect the SuHV-1 and also to differentiate genotypes from brain tissue of infected pigs. The BamHI-PCR is a rapid, practical, and sensitive way to genotype SuHV-1. |
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Palavras-Chave: |
PCR. |
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Thesagro: |
Genótipo; Vírus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01560naa a2200253 a 4500
001 1919948
005 2012-03-22
008 2011 bl uuuu u00u1 u #d
100 1 $aFONSECA JÚNIOR, A. A.
245 $aGenotyping of the Pseudorabies vírus by multiplex PCR followed by restriction enzyme analysis.$h[electronic resource]
260 $c2011
500 $aProjeto/Plano de Ação: 03.09.00.046.
520 $aSuid herpesvirus 1 (SuHV-1) is the causative agent of Aujeszky?s disease. The infectious agent has only one serotype, but it was classified by restriction enzyme analysis of the whole genome into four genotypes, named I to IV. The aim of this study was to standardize a rapid method for genotyping SuHV-1 without virus isolation, using a multiplex-PCR followed by enzymatic restriction analysis. The complete genome of the virus was analyzed in silico to determine the restriction sites for the enzyme BamHI. Primers were designed to flank sites with emphasis on certain points of differentiation of genotypes. The standard PCRs were able to detect the SuHV-1 and also to differentiate genotypes from brain tissue of infected pigs. The BamHI-PCR is a rapid, practical, and sensitive way to genotype SuHV-1.
650 $aGenótipo
650 $aVírus
653 $aPCR
700 1 $aMAGALHÃES, C. G.
700 1 $aSALES, E. B.
700 1 $aD'AMBROS, R. M.
700 1 $aZANELLA, J. R. C.
700 1 $aHEINEMANN, M. B.
700 1 $aLEITE, R. C.
700 1 $aREIS, J. K. P. dos
773 $tISRN Microbiology$gv. 2011. Doi:10.5402/2011/458294.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Registro |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
05/11/1996 |
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Data da última atualização: |
03/04/2017 |
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Autoria: |
CATELLA, A. C.; PETRERE JUNIOR, M. |
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Afiliação: |
EMBRAPA. Centro de Pesquisa Agropecuaria do Pantanal (Corumba, MS); UNESP. Departamento de Ecologia (Rio Claro, SP). |
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Título: |
Feeding patterns in a fish community of Baia da Onca, a floodplain lake of the Aquidauana River, Pantanal, Brazil. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Fisheries Management and Ecology, v.3, p.229-237, 1996. |
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Idioma: |
Inglês |
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Conteúdo: |
The stomach contents of 24 species of fish and unidentified species from four genera in a floodplain lake of the Pantanal were studied. Fish were collected during the dry season when the lake was isolated from the main river. A community food ingestion index (feeding index weighted by consumer biomass, FIWCB, where 0 < FIWCB < 1)was calculated to quantify feeding patterns. Detritus and algae ingested by Curimatidae were responsible for 46,5% of the FIWCB, while that for algae ingested by Loricariidae (armoured catfish) accounted for 13,2%. The importance of detritus in fish diets is discussed as a strategy for shortening food chains, thereby increasing community efficiency, and as a consequence, fish biomass. It was learnt that the main route of energy flow inthis perennial lake was through the detritus chain. The floodplain lakes function as a dry season feeding ground for small-sized species of fish, which are potential prey for the more highly valued larger species of fish. |
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Palavras-Chave: |
Alimentacao de comunidade; Conteudo estomacal; Feeding index; Fish community feeding; Indice alimentar; Stomach contents. |
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Thesagro: |
Cadeia Alimentar; Peixe. |
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Thesaurus NAL: |
Brazil; food chain; Pantanal. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01766naa a2200265 a 4500
001 1789891
005 2017-04-03
008 1996 bl --- 0-- u #d
100 1 $aCATELLA, A. C.
245 $aFeeding patterns in a fish community of Baia da Onca, a floodplain lake of the Aquidauana River, Pantanal, Brazil.
260 $c1996
520 $aThe stomach contents of 24 species of fish and unidentified species from four genera in a floodplain lake of the Pantanal were studied. Fish were collected during the dry season when the lake was isolated from the main river. A community food ingestion index (feeding index weighted by consumer biomass, FIWCB, where 0 < FIWCB < 1)was calculated to quantify feeding patterns. Detritus and algae ingested by Curimatidae were responsible for 46,5% of the FIWCB, while that for algae ingested by Loricariidae (armoured catfish) accounted for 13,2%. The importance of detritus in fish diets is discussed as a strategy for shortening food chains, thereby increasing community efficiency, and as a consequence, fish biomass. It was learnt that the main route of energy flow inthis perennial lake was through the detritus chain. The floodplain lakes function as a dry season feeding ground for small-sized species of fish, which are potential prey for the more highly valued larger species of fish.
650 $aBrazil
650 $afood chain
650 $aPantanal
650 $aCadeia Alimentar
650 $aPeixe
653 $aAlimentacao de comunidade
653 $aConteudo estomacal
653 $aFeeding index
653 $aFish community feeding
653 $aIndice alimentar
653 $aStomach contents
700 1 $aPETRERE JUNIOR, M.
773 $tFisheries Management and Ecology$gv.3, p.229-237, 1996.
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Embrapa Pantanal (CPAP) |
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