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| 11. |  | FERREIRA, J. A. M.; VENZON, M.; CARVALHO, V. F. Flutuacao populacional de cigarrinhas-de-pastagens no municipio de Uberaba, Minas Gerais. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 17.; ENCONTRO NACIONAL DE FITOSSANITARISTAS, 8., 1998, Rio de Janeiro, RJ. Resumos. [Rio de Janeiro]: UFRRJ, 1998. p.249.| Biblioteca(s): Embrapa Cerrados. |
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| 13. | | PAULA JÚNIOR, T. J. de; VENZON, M.; MORANDI, M. A. B.; BETTIOL, W.; TEIXEIRA, H. Comercialização de produtos biológicos para o controle de doenças de plantas e pragas no Brasil. Informe Agropecuário, Belo Horizonte, v. 30, n. 251, p. 16-123, jul./ago.2009.| Biblioteca(s): Embrapa Meio Ambiente. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
01/08/2013 |
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Data da última atualização: |
01/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CLEMENTE, Z.; CASTRO, V. L. S. S. de; FEITOSA, L. O.; LIMA, R.; JONSSON, C. M.; MAIA, A. de H. N.; FRACETO, L. F. |
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Afiliação: |
ZAIRA CLEMENTE, IB-UNICAMP; VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA; L. O. FEITOSA, UFSCar; R. LIMA, UFSCar; CLAUDIO MARTIN JONSSON, CNPMA; ALINE DE HOLANDA NUNES MAIA, CNPMA; LEONARDO FERNANDES FRACETO, UNESP. |
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Título: |
Fish exposure to nano-TiO2 under different experimental conditions: methodological aspects for nanoecotoxicology investigations. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Science of the Total Environment, Amsterdam, v. 463-464, p. 647-656, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
The ecotoxicology of nano-TiO2 has been extensively studied in recent years; however, few toxicological investigations have considered the photocatalytic properties of the substance, which can increase its toxicity to aquatic biota. The aim of this work was to evaluate the effects on fish exposed to different nano-TiO2 concentrations and illumination conditions. The interaction of these variables was investigated by observing the survival of the organisms, together with biomarkers of biochemical and genetic alterations. Fish (Piaractus mesopotamicus) were exposed for 96 h to 0, 1, 10, and 100 mg/L of nano-TiO2, under visible light, and visible light with ultraviolet (UV) light (22.47 J/cm2/h). The following biomarkers of oxidative stress were monitored in the liver: concentrations of lipid hydroperoxide and carbonylated protein, and specific activities of superoxide dismutase, catalase, and glutathione S-transferase. Other biomarkers of physiological function were also studied: the specific activities of acid phosphatase and Na,K-ATPase were analyzed in the liver and brain, respectively, and the concentration of metallothionein was measured in the gills. In addition, micronucleus and comet assays were performed with blood as genotoxic biomarkers. Nano-TiO2 caused no mortality under any of the conditions tested, but induced sublethal effects that were influenced by illumination condition. Under both illumination conditions tested, exposure to 100 mg/L showed an inhibition of acid phosphatase activity. Under visible light, there was an increase in metallothionein level in fish exposed to 1 mg/L of nano-TiO2. Under UV light, protein carbonylation was reduced in groups exposed to 1 and 10 mg/L, while nucleus alterations in erythrocytes were higher in fish exposed to 10 mg/L. As well as improving the understanding of nano-TiO2 toxicity, the findings demonstrated the importance of considering the experimental conditions in nanoecotoxicological tests. This work provides information for the development of protocols to study substances whose toxicity is affected by illumination conditions. MenosThe ecotoxicology of nano-TiO2 has been extensively studied in recent years; however, few toxicological investigations have considered the photocatalytic properties of the substance, which can increase its toxicity to aquatic biota. The aim of this work was to evaluate the effects on fish exposed to different nano-TiO2 concentrations and illumination conditions. The interaction of these variables was investigated by observing the survival of the organisms, together with biomarkers of biochemical and genetic alterations. Fish (Piaractus mesopotamicus) were exposed for 96 h to 0, 1, 10, and 100 mg/L of nano-TiO2, under visible light, and visible light with ultraviolet (UV) light (22.47 J/cm2/h). The following biomarkers of oxidative stress were monitored in the liver: concentrations of lipid hydroperoxide and carbonylated protein, and specific activities of superoxide dismutase, catalase, and glutathione S-transferase. Other biomarkers of physiological function were also studied: the specific activities of acid phosphatase and Na,K-ATPase were analyzed in the liver and brain, respectively, and the concentration of metallothionein was measured in the gills. In addition, micronucleus and comet assays were performed with blood as genotoxic biomarkers. Nano-TiO2 caused no mortality under any of the conditions tested, but induced sublethal effects that were influenced by illumination condition. Under both illumination conditions tested, exposure to 100 mg/L showed an inhibition of ... Mostrar Tudo |
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Palavras-Chave: |
Nanotoxicity. |
|
Thesagro: |
Pacu; Peixe; Substância tóxica; Toxidez. |
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Thesaurus NAL: |
Biomarkers; Ecotoxicology; Fish; Nanotechnology; Oxidative stress; Piaractus mesopotamicus; Titanium dioxide; Ultraviolet radiation. |
|
Categoria do assunto: |
H Saúde e Patologia |
|
Marc: |
LEADER 03141naa a2200349 a 4500
001 1963123
005 2023-03-01
008 2013 bl uuuu u00u1 u #d
100 1 $aCLEMENTE, Z.
245 $aFish exposure to nano-TiO2 under different experimental conditions$bmethodological aspects for nanoecotoxicology investigations.$h[electronic resource]
260 $c2013
520 $aThe ecotoxicology of nano-TiO2 has been extensively studied in recent years; however, few toxicological investigations have considered the photocatalytic properties of the substance, which can increase its toxicity to aquatic biota. The aim of this work was to evaluate the effects on fish exposed to different nano-TiO2 concentrations and illumination conditions. The interaction of these variables was investigated by observing the survival of the organisms, together with biomarkers of biochemical and genetic alterations. Fish (Piaractus mesopotamicus) were exposed for 96 h to 0, 1, 10, and 100 mg/L of nano-TiO2, under visible light, and visible light with ultraviolet (UV) light (22.47 J/cm2/h). The following biomarkers of oxidative stress were monitored in the liver: concentrations of lipid hydroperoxide and carbonylated protein, and specific activities of superoxide dismutase, catalase, and glutathione S-transferase. Other biomarkers of physiological function were also studied: the specific activities of acid phosphatase and Na,K-ATPase were analyzed in the liver and brain, respectively, and the concentration of metallothionein was measured in the gills. In addition, micronucleus and comet assays were performed with blood as genotoxic biomarkers. Nano-TiO2 caused no mortality under any of the conditions tested, but induced sublethal effects that were influenced by illumination condition. Under both illumination conditions tested, exposure to 100 mg/L showed an inhibition of acid phosphatase activity. Under visible light, there was an increase in metallothionein level in fish exposed to 1 mg/L of nano-TiO2. Under UV light, protein carbonylation was reduced in groups exposed to 1 and 10 mg/L, while nucleus alterations in erythrocytes were higher in fish exposed to 10 mg/L. As well as improving the understanding of nano-TiO2 toxicity, the findings demonstrated the importance of considering the experimental conditions in nanoecotoxicological tests. This work provides information for the development of protocols to study substances whose toxicity is affected by illumination conditions.
650 $aBiomarkers
650 $aEcotoxicology
650 $aFish
650 $aNanotechnology
650 $aOxidative stress
650 $aPiaractus mesopotamicus
650 $aTitanium dioxide
650 $aUltraviolet radiation
650 $aPacu
650 $aPeixe
650 $aSubstância tóxica
650 $aToxidez
653 $aNanotoxicity
700 1 $aCASTRO, V. L. S. S. de
700 1 $aFEITOSA, L. O.
700 1 $aLIMA, R.
700 1 $aJONSSON, C. M.
700 1 $aMAIA, A. de H. N.
700 1 $aFRACETO, L. F.
773 $tScience of the Total Environment, Amsterdam$gv. 463-464, p. 647-656, 2013.
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