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Registro Completo |
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
11/12/2017 |
Data da última atualização: |
11/12/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
COELHO, S. V. B.; ROSA, S. D. V. F. da; CLEMENTE, A. da C. S.; PEREIRA, C. C.; FIGUEIREDO, M. A. de; REIS, L. V. |
Afiliação: |
STEFANIA VILAS BOAS COELHO, UFLA; STTELA DELLYZETE VEIGA F DA ROSA, SAPC; ALINE DA CONSOLAÇÃO SAMPAIO CLEMENTE, UFLA; CRISTIANE CARVALHO PEREIRA, UFLA; MADELEINE ALVES DE FIGUEIREDO, UFLA; LEANDRO VILELA REIS, UFLA. |
Título: |
Tolerance of Coffea arabica L. seeds to sub zero temperatures. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Ciência e Agrotecnologia, v. 41, n. 3, p. 312-321, May/Jun. 2017. |
Idioma: |
Inglês |
Notas: |
Título em português: Tolerância de sementes de Coffea arabica L. à temperaturas sub zero. |
Conteúdo: |
Preservation of the quality of coffee seeds is hindered by their intermediate behavior in storage. However, long-term storage at sub zero temperatures may be achieved by adjusting the water content of the seeds. The aim of this study was to evaluate the tolerance of coffee seeds to freezing, in relation to physiological and enzymatic modifications. Coffee seeds were dried in two manners, rapid and slow, to water contents of interest, 0.67, 0.43, 0.25, 0.18, 0.11, and 0.05 g H2O g-¹ dw (dry basis). After drying, the seeds were stored at a temperature of -20 ºC and of 86 ºC for 24 hours and for 12 months, and then compared to seeds in cold storage at 10 ºC. The seeds were evaluated through calculation of percentage of normal seedlings, percentage of seedlings with expanded cotyledonary leaves, dry matter of roots and of hypocotyls, and viability of embryos in the tetrazolium test. Expression of the enzymes superoxide dismutase, catalase, and peroxidase were evaluated by means of electrophoretic analysis. Only seeds dried more slowly to 0.18 g H2O g-1 dw present relative tolerance to storing at -20 °C for 12 months. Coffee seeds do not tolerate storage at a temperature of -86 ºC for 12 months. Water contents below 0.11g H2O g-¹ dw and above 0.43 g H2O g-¹ dw hurt the physiological quality of coffee seeds, regardless of the type of drying, temperature, and storage period. Coffee seed embryos are more tolerant to desiccation and to freezing compared to whole seeds, especially when the seeds are dried to 0.05 g H2O g-¹ dw. The catalase enzyme can be used as a biochemical marker to study tolerance to freezing in coffee seeds. MenosPreservation of the quality of coffee seeds is hindered by their intermediate behavior in storage. However, long-term storage at sub zero temperatures may be achieved by adjusting the water content of the seeds. The aim of this study was to evaluate the tolerance of coffee seeds to freezing, in relation to physiological and enzymatic modifications. Coffee seeds were dried in two manners, rapid and slow, to water contents of interest, 0.67, 0.43, 0.25, 0.18, 0.11, and 0.05 g H2O g-¹ dw (dry basis). After drying, the seeds were stored at a temperature of -20 ºC and of 86 ºC for 24 hours and for 12 months, and then compared to seeds in cold storage at 10 ºC. The seeds were evaluated through calculation of percentage of normal seedlings, percentage of seedlings with expanded cotyledonary leaves, dry matter of roots and of hypocotyls, and viability of embryos in the tetrazolium test. Expression of the enzymes superoxide dismutase, catalase, and peroxidase were evaluated by means of electrophoretic analysis. Only seeds dried more slowly to 0.18 g H2O g-1 dw present relative tolerance to storing at -20 °C for 12 months. Coffee seeds do not tolerate storage at a temperature of -86 ºC for 12 months. Water contents below 0.11g H2O g-¹ dw and above 0.43 g H2O g-¹ dw hurt the physiological quality of coffee seeds, regardless of the type of drying, temperature, and storage period. Coffee seed embryos are more tolerant to desiccation and to freezing compared to whole seeds, especially whe... Mostrar Tudo |
Palavras-Chave: |
Antioxidant enzymes; Enzima antioxidante; Saturated saline solutions; Solução salina saturada. |
Thesagro: |
Congelamento; Secagem. |
Thesaurus Nal: |
Drying; Freezing; Silica gel. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/168585/1/Tolerance-of-Coffea-arabica-L.-seeds.pdf
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Marc: |
LEADER 02600naa a2200301 a 4500 001 2082056 005 2017-12-11 008 2017 bl uuuu u00u1 u #d 100 1 $aCOELHO, S. V. B. 245 $aTolerance of Coffea arabica L. seeds to sub zero temperatures.$h[electronic resource] 260 $c2017 500 $aTítulo em português: Tolerância de sementes de Coffea arabica L. à temperaturas sub zero. 520 $aPreservation of the quality of coffee seeds is hindered by their intermediate behavior in storage. However, long-term storage at sub zero temperatures may be achieved by adjusting the water content of the seeds. The aim of this study was to evaluate the tolerance of coffee seeds to freezing, in relation to physiological and enzymatic modifications. Coffee seeds were dried in two manners, rapid and slow, to water contents of interest, 0.67, 0.43, 0.25, 0.18, 0.11, and 0.05 g H2O g-¹ dw (dry basis). After drying, the seeds were stored at a temperature of -20 ºC and of 86 ºC for 24 hours and for 12 months, and then compared to seeds in cold storage at 10 ºC. The seeds were evaluated through calculation of percentage of normal seedlings, percentage of seedlings with expanded cotyledonary leaves, dry matter of roots and of hypocotyls, and viability of embryos in the tetrazolium test. Expression of the enzymes superoxide dismutase, catalase, and peroxidase were evaluated by means of electrophoretic analysis. Only seeds dried more slowly to 0.18 g H2O g-1 dw present relative tolerance to storing at -20 °C for 12 months. Coffee seeds do not tolerate storage at a temperature of -86 ºC for 12 months. Water contents below 0.11g H2O g-¹ dw and above 0.43 g H2O g-¹ dw hurt the physiological quality of coffee seeds, regardless of the type of drying, temperature, and storage period. Coffee seed embryos are more tolerant to desiccation and to freezing compared to whole seeds, especially when the seeds are dried to 0.05 g H2O g-¹ dw. The catalase enzyme can be used as a biochemical marker to study tolerance to freezing in coffee seeds. 650 $aDrying 650 $aFreezing 650 $aSilica gel 650 $aCongelamento 650 $aSecagem 653 $aAntioxidant enzymes 653 $aEnzima antioxidante 653 $aSaturated saline solutions 653 $aSolução salina saturada 700 1 $aROSA, S. D. V. F. da 700 1 $aCLEMENTE, A. da C. S. 700 1 $aPEREIRA, C. C. 700 1 $aFIGUEIREDO, M. A. de 700 1 $aREIS, L. V. 773 $tCiência e Agrotecnologia$gv. 41, n. 3, p. 312-321, May/Jun. 2017.
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Embrapa Café (CNPCa) |
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
Data corrente: |
30/11/2015 |
Data da última atualização: |
06/01/2016 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SERAFIM, C. M.; CASTRO, A. C. R. de; CARVALHO, A. C. P. P. de; BERTINI, C. H. C. de M. |
Afiliação: |
Cecília Moreira Serafim, Universidade Federal do Ceará; ANA CECILIA RIBEIRO DE CASTRO, CNPAT; ANA CRISTINA PORTUGAL P DE CARVALHO, CNPAT; Cândida Hermínia Campos de Magalhães Bertini, Universidade Federal do Ceará. |
Título: |
Micropropagação de Anthurium maricense por meio da indução ao estiolamento. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AGROINDÚSTRIA TROPICAL, 12., 2014, Fortaleza. Resumos... Fortaleza: Embrapa Agroindústria Tropical, 2015. |
Idioma: |
Português |
Palavras-Chave: |
Cultura de tecidos. |
Thesagro: |
Antúrio; Organogénese. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/134287/1/RES15010.pdf
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Marc: |
LEADER 00630nam a2200169 a 4500 001 2029959 005 2016-01-06 008 2014 bl uuuu u00u1 u #d 100 1 $aSERAFIM, C. M. 245 $aMicropropagação de Anthurium maricense por meio da indução ao estiolamento. 260 $aIn: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AGROINDÚSTRIA TROPICAL, 12., 2014, Fortaleza. Resumos... Fortaleza: Embrapa Agroindústria Tropical$c2015 650 $aAntúrio 650 $aOrganogénese 653 $aCultura de tecidos 700 1 $aCASTRO, A. C. R. de 700 1 $aCARVALHO, A. C. P. P. de 700 1 $aBERTINI, C. H. C. de M.
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