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| Registros recuperados : 95 | |
| 42. |  | SILVA, V. F. da; VENTURIN, N.; OLIVEIRA FILHO, A. T. de; MACEDO, R. L. G. de; CARVALHO, W. A. C.; BERG, E. van den. Caracterização estrutural de um fragmento de floresta semidecídua no município de Ibituruna, MG. Cerne, Lavras, v. 9, n. 1, p. 92-106, 2003.| Biblioteca(s): Embrapa Florestas. |
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| 44. | | MEDEIROS, G. X. DE; VIEIRA, F.; OLIVEIRA, T.; PAIVA, R.; BARBOSA, M.; MACHADO, M. A.; MARTINS, M. F.; BRANDAO, H. de M.; CARVALHO, W. A. Desenvolvimento de metodologia de cultivo de hepatócitos bovinos in vitro para estudos moleculares e bioquímicos. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 20. 2017, 4 p. Anais... Juiz de Fora/MG. Embrapa Gado de Leite.| Biblioteca(s): Embrapa Gado de Leite. |
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| 45. | | PEREIRA, E. M. C.; CAMPOS, M. M.; SILVA, A. S.; DAVIÃO, J.; NEVES, L. F. M.; GOMES, M. B.; PRATA, M. C. de A.; CARVALHO, W. A. Desenvolvimento de nanossistemas imunomoduladores para aplicação no controle do carrapato bovino. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE PIBIC/CNPQ, 26., 2022, Juiz de Fora. Anais... Juiz de Fora: Embrapa Gado de Leite, 2022. p. 101-104.| Biblioteca(s): Embrapa Gado de Leite. |
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| 46. | | FERREIRA, S. E.; GOMES, M. B.; NEVES, L. F. M.; LOURES, A. R. S.; SILVA, A. S.; DIAVÃO, J.; COELHO, S. G.; CARVALHO, W. A.; CAMPOS, M. M. Efeitos da amplitude termica nos parâmetros fisiologicos de bezerros nos primeiros 28 dias de idade. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE PIBIC/CNPQ, 26., 2022, Juiz de Fora. Anais... Juiz de Fora: Embrapa Gado de Leite, 2022. p. 115-119.| Biblioteca(s): Embrapa Gado de Leite. |
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| 47. | | CARVALHO, W. A.; DOMINGUES, R.; PRATA, M. C. de A.; SILVA, M. V. G. B.; GUIMARÃES, S. E. F.; MACHADO, M. A. Microarray analysis of tick-infested skin in resistant and susceptible cattle confirms the role of inflammatory pathways in immune activation and larval rejection. Veterinary Parasitology, Amsterdam, v. 205, n. 1?2, p. 307?317, Sept. 2014.| Biblioteca(s): Embrapa Pecuária Sul. |
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| 48. | | CARVALHO, W. A.; DOMINGUES, R.; PRATA, M. C. de A.; SILVA, M. V. G. B.; OLIVEIRA, G. C. de; GUIMARÃES, S. E. F.; MACHADO, M. A. Microarray analysis of tick-infested skin in resistant and susceptible cattle confirms the role of inflammatory pathways in immune activation and larval rejection. Veterinary Parasitology, Amsterdam, v. 205, n. 1/2, p. 307-317, 2014.| Biblioteca(s): Embrapa Gado de Leite. |
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| 50. | | CARVALHO, W. A.; GERN, J. C.; GUIMARAES, A. S.; BRAYNER, R.; GUELBER, G. T.; SALES, E. B.; MACHADO, M. A.; SILVA, S. R.; BRANDAO, H. de M. Toxicity of barium titanate in bovine fibroblasts. In: ENCONTRO DA SOCIEDADE BRASILEIRA DE PESQUISA EM MATERIAIS, SBPMAT, 12., 2013, Campos do Jordão. Resumos. Campos do Jordão: SBPMat, 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 51. | | GERN, J. C.; RIBEIRO, J. B.; CARVALHO, W. A.; SOUZA, G. N. de; SILVA, M. R.; GUIMARAES, A. S.; BRANDAO, H. de M. Terapêutica de precisão e suas perspectivas em um país de pecuária heterogênea. In: ZOCCAL, R.; PEREIRA, V. da F. (Coord.). PANORAMA DO LEITE. Boletim Eletrônico Mensal, v. 8, n. 86, 2016. Juiz de Fora: Embrapa Gado de Leite, abr. 2016. p. 10-11| Biblioteca(s): Embrapa Gado de Leite. |
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| 54. | | SOUZA, G. N. de; GREGO, C. R.; HOTT, M. C.; SILVA, M. R.; CARVALHO, W. A.; HYLARIO, S. M.; AMARAL, C. M.; OLIVEIRA, E. F. DE. Spatial analysis of bulk tank milk somatic cell counts from dairy herds located in Brazil Southeast Region, 2011. In: CONGRESSO INTERNACIONAL DO LEITE, 11.; WORKSHOP DE POLÍTICAS PÚBLICAS, 11.; SIMPÓSIO DE SUSTENTABILIDADE DA ATIVIDADE LEITEIRA, 12., 2012, Goiânia. Anais... Juiz de Fora: Embrapa Gado de Leite, 2012.| Biblioteca(s): Embrapa Gado de Leite. |
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| 55. | | SOUZA, G. N. de; GREGO, C. R.; HOTT, M. C.; SILVA, M. R.; CARVALHO, W. A.; HYLARIO, S. M.; AMARAL, C. M.; OLIVEIRA, E. F. DE. Spatial analysis of bulk tank milk somatic cell counts from dairy herds located in southeast region of Brazil, 2011. In: NATIONAL MASTITIS COUNCIL ANNUAL MEETING, 52, 2013, San Diego. Proceedings... San Diego: [s.n.], 2013. p. 207-208.| Biblioteca(s): Embrapa Gado de Leite. |
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| 56. | | CARVALHO, W. A.; BECHARA, G. H.; MORÉ, D. D.; FERREIRA, B. R.; SILVA, J. S. da; SANTOS, I. K. F. de M. S. Rhipicephalus (Boophilus) microplus: distinct acute phase proteins vary during infestations according to the genetic composition of the bovine hosts, Bos taurus and Bos indicus. Experimental Parasitology, v. 118, p. 587-591, 2008.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 57. | | DIAS, B.; ABREU, C. P. V.; MAIA, J. M. S.; SILVA, M. R.; SOUZA, G. N. de; RIBEIRO, J. B.; BRANDAO, H. de M.; GUIMARAES, A. S.; CARVALHO, W. A. Aumento da eficácia do tratamento da ceratoconjuntivite com formulação mucoadesiva nanoparticulada envolve modulação da resposta humoral em bovinos infectados com Moraxella bovis. In: CONGRESSO INTERNACIONAL DO LEITE, 13., 2015, Porto Alegre. Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. 4 p.| Biblioteca(s): Embrapa Gado de Leite. |
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| 58. | | DIAS, B.; ABREU, C. P. V.; MAIA, J. M. S.; SILVA, M. R.; SOUZA, G. N. de; RIBEIRO, J. B.; BRANDAO, H. de M.; GUIMARAES, A. S.; CARVALHO, W. A. Aumento da eficácia do tratamento da ceratoconjuntivite com formulação mucoadesiva nanoparticulada envolve modulação da resposta humoral em bovinos infectados com Moraxella bovis. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 16., 2015, Juiz de Fora, MG Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. 1 CD-ROM. (Embrapa Gado de Leite. Documentos, 185.). 2 p.| Biblioteca(s): Embrapa Gado de Leite. |
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| 59. | | CARVALHO, B. de O.; PEREIRA, H. P.; SEIBERLICK, O. S. G.; REIS, D. R. de L.; CARVALHO, W. A.; SILVA, M. V. G. B.; MACHADO, M. A.; MARTINS, M. F. Análise de genes diferencialmente expressos em resposta à infecção por Streptococcus agalactiae. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 24., 2019, Juiz de Fora. Anais... Juiz de Fora: Embrapa Gado de Leite, 2019. Editor Técnico: Leônidas Paixão Passos, Embrapa Gado de Leite.| Biblioteca(s): Embrapa Gado de Leite. |
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| 60. | | FONSECA, I.; PINTO, I. S. B.; MENDONÇA, J. F. M. de; VALVERDE, I. D.; BRANDAO, H. de M.; GUIMARAES, A. S.; CARVALHO, W. A.; VICCINI, L. F.; MARTINS, M. F. Análise do perfil de expressão dos genes IL-1beta e IL-10 em úberes extracorpóreos em resposta à Streptococcus agalactiae. In: CONGRESSO INTERNACIONAL DA RAÇA GIROLANDO, 1.; CONGRESSO BRASILEIRO DA RAÇA GIROLANDO, 2., 2015, Belo Horizonte. Anais... Belo Horizonte: Associação Brasileira dos Criadores de Girolando, 2015. 5 p.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 95 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
29/12/2019 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
WELLER, M. M. D. C. A.; FONSECA, I.; SBARDELLA, A. P.; PINTO, I. S. B.; VICCINI, L. F.; BRANDAO, H. de M.; GERN, J. C.; CARVALHO, W. A.; GUIMARÃES, A. S.; BRITO, M. A. V. P.; MUNARI, D. P.; SILVA, M. V. G. B.; MARTINS, M. F. |
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Afiliação: |
HUMBERTO DE MELLO BRANDAO, CNPGL; JULIANA CARINE GERN, CNPGL; WANESSA ARAUJO CARVALHO, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; MARTA FONSECA MARTINS, CNPGL. |
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Título: |
Isolated perfused udder model fortranscriptome analysis in response to Streptococcus agalactiae. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Dairy Research, v. 86, n. 3, p. 307-314, 2019. |
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DOI: |
https://doi.org/10.1017/s0022029919000451 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to evaluate the transcriptional changes occurring in isolated perfused mammary alveolar tissue in response to inoculation with S. agalactiae and to identify the most affected biological functions and pathways after 3 h. Four udders taken at slaughter from cows with healthy mammary gland were perfused ex situ with warmed and gassed Tyrode's solution. Mammary alveolar tissue samples were taken from the left fore and rear quarters (IQ-inoculated quarters) before inoculation (hour 0) and at 3 h post inoculation (hpi) and at the same times from control right fore and rear quarters (not inoculated: NIQ). A total of 1756 differentially expressed genes (DEGs) were identified between IQ and NIQ at 3 hpi using edgeR package. Within this set of DEGs, 952 were up regulated and mainly involved with innate immune response and inflammatory response, e.g., CD14, CCL5, TLR2, IL-8, SAA3, as well as in transcriptional regulation such as FOS, STAT3 and NFKBIA. Genes down-regulated (804) included those involved with lipid synthesis e.g., APOC2, SCD, FABP3 and FABP4. The most affected pathways were chemokine signaling, Wnt signaling and complement and coagulation cascades, which likely reflects the early stage response of mammary tissue to S. agalactiae infection. No significant gene expression changes were detected by RNA-Seq in the others contrasts. Real time-PCR confirmed the increase in mRNA abundance of immune-related genes: TLR2, TLR4, IL-1β, and IL-10 at 3 hpi between IQ and NIQ. The expression profiles of Casp1 and Bax for any contrasts were unaffected whereas Bcl2 was increased in IQ, which suggests no induction of apoptosis during the first hours after infection. Results provided novel information regarding the early functional pathways and gene network that orchestrate innate immune responses to S. agalactiae infection. This knowledge could contribute to new strategies to enhance resistance to this disease, such as genomic selection. MenosThis study aimed to evaluate the transcriptional changes occurring in isolated perfused mammary alveolar tissue in response to inoculation with S. agalactiae and to identify the most affected biological functions and pathways after 3 h. Four udders taken at slaughter from cows with healthy mammary gland were perfused ex situ with warmed and gassed Tyrode's solution. Mammary alveolar tissue samples were taken from the left fore and rear quarters (IQ-inoculated quarters) before inoculation (hour 0) and at 3 h post inoculation (hpi) and at the same times from control right fore and rear quarters (not inoculated: NIQ). A total of 1756 differentially expressed genes (DEGs) were identified between IQ and NIQ at 3 hpi using edgeR package. Within this set of DEGs, 952 were up regulated and mainly involved with innate immune response and inflammatory response, e.g., CD14, CCL5, TLR2, IL-8, SAA3, as well as in transcriptional regulation such as FOS, STAT3 and NFKBIA. Genes down-regulated (804) included those involved with lipid synthesis e.g., APOC2, SCD, FABP3 and FABP4. The most affected pathways were chemokine signaling, Wnt signaling and complement and coagulation cascades, which likely reflects the early stage response of mammary tissue to S. agalactiae infection. No significant gene expression changes were detected by RNA-Seq in the others contrasts. Real time-PCR confirmed the increase in mRNA abundance of immune-related genes: TLR2, TLR4, IL-1β, and IL-10 at 3 hpi between IQ a... Mostrar Tudo |
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Palavras-Chave: |
Mammary gland perfused; RNA-Seq. |
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Thesaurus NAL: |
Dairy cattle; Immune response; Mastitis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02978naa a2200337 a 4500
001 2117822
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/s0022029919000451$2DOI
100 1 $aWELLER, M. M. D. C. A.
245 $aIsolated perfused udder model fortranscriptome analysis in response to Streptococcus agalactiae.$h[electronic resource]
260 $c2019
520 $aThis study aimed to evaluate the transcriptional changes occurring in isolated perfused mammary alveolar tissue in response to inoculation with S. agalactiae and to identify the most affected biological functions and pathways after 3 h. Four udders taken at slaughter from cows with healthy mammary gland were perfused ex situ with warmed and gassed Tyrode's solution. Mammary alveolar tissue samples were taken from the left fore and rear quarters (IQ-inoculated quarters) before inoculation (hour 0) and at 3 h post inoculation (hpi) and at the same times from control right fore and rear quarters (not inoculated: NIQ). A total of 1756 differentially expressed genes (DEGs) were identified between IQ and NIQ at 3 hpi using edgeR package. Within this set of DEGs, 952 were up regulated and mainly involved with innate immune response and inflammatory response, e.g., CD14, CCL5, TLR2, IL-8, SAA3, as well as in transcriptional regulation such as FOS, STAT3 and NFKBIA. Genes down-regulated (804) included those involved with lipid synthesis e.g., APOC2, SCD, FABP3 and FABP4. The most affected pathways were chemokine signaling, Wnt signaling and complement and coagulation cascades, which likely reflects the early stage response of mammary tissue to S. agalactiae infection. No significant gene expression changes were detected by RNA-Seq in the others contrasts. Real time-PCR confirmed the increase in mRNA abundance of immune-related genes: TLR2, TLR4, IL-1β, and IL-10 at 3 hpi between IQ and NIQ. The expression profiles of Casp1 and Bax for any contrasts were unaffected whereas Bcl2 was increased in IQ, which suggests no induction of apoptosis during the first hours after infection. Results provided novel information regarding the early functional pathways and gene network that orchestrate innate immune responses to S. agalactiae infection. This knowledge could contribute to new strategies to enhance resistance to this disease, such as genomic selection.
650 $aDairy cattle
650 $aImmune response
650 $aMastitis
653 $aMammary gland perfused
653 $aRNA-Seq
700 1 $aFONSECA, I.
700 1 $aSBARDELLA, A. P.
700 1 $aPINTO, I. S. B.
700 1 $aVICCINI, L. F.
700 1 $aBRANDAO, H. de M.
700 1 $aGERN, J. C.
700 1 $aCARVALHO, W. A.
700 1 $aGUIMARÃES, A. S.
700 1 $aBRITO, M. A. V. P.
700 1 $aMUNARI, D. P.
700 1 $aSILVA, M. V. G. B.
700 1 $aMARTINS, M. F.
773 $tJournal of Dairy Research$gv. 86, n. 3, p. 307-314, 2019.
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