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| Registros recuperados : 23 | |
| 1. |  | LOPES, V. S.; CARVALHO, M. C. C. G. de; DIAS, W. P.; MARCELINO-GUIMARÃES, F. C. Genes Hsp20 de soja: organização no genoma e categorização entre subfamílias. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: resumos. Brasília, DF: Embrapa, 2012. p. 17, res. 12.| Biblioteca(s): Embrapa Soja. |
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| 4. | | CARVALHO, K. de; RINCÃO, M. P.; DARBEN, L. M.; CARVALHO, M. C. C. G. de; LOPES, I. de O. N.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. External application of dsRNA targeting a Phakopsora pachyrhizi effector candidate attenuated fungal pathogenicity. In: INTERNATIONAL PLANT & ANIMAL GENOME CONFERENCE, 25., 2017, San Diego. Abstracts... [S. l.: s. n.], 2017. não paginado. PLANT AND ANIMAL GENOME XXV CONFERENCE - INTLPAG, 2017.| Biblioteca(s): Embrapa Soja. |
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| 5. | | LOPES, V. S.; FUGANTI, R.; BARBOSA, J. F.; DE CARVALHO, M. C. C. G.; MARIN, S. R. R.; SILVA, J. F. V.; NEPOMUCENO, A. L.; MARCELINO, F. C. Estudos in vivo do papel de inserções AT(n) da região promotora do gene Gmhsp17.6-L na regulação de genes em soja. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 4., 2009, Londrina. Resumos... Londrina: Embrapa Soja, 2009. p. 169-174. (Embrapa Soja. Documentos, 312). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo.| Biblioteca(s): Embrapa Soja. |
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| 6. | | DARBEN, L. M.; QI, M.; YOKOYAMA, A.; CARVALHO, M. C. C. G. de; ABDELNOOR, R. V.; WHITHAM, S. A.; MARCELINO-GUIMARÃES, F. C. Functional characterization of P. pachyrhizi effector candidates. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls. Abstract... Abstract: 840.pdf.| Biblioteca(s): Embrapa Soja. |
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| 7. | | UTIYAMA, A. S.; ABE, V. Y.; DARBEN, L. M.; CARVALHO, K.; CARVALHO, M. C. C. G. de; MARCELINO-GUIMARÃES, F. C. Avaliação funcional de proteínas candidatas a efetores de Phakopsora pachyrhizi por meio da análise de espécies reativas a oxigênio (ROS) e deposição de calose. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 13., 2018, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2018. p. 38-45.| Biblioteca(s): Embrapa Soja. |
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| 8. | | POLIZEL-PODANOSQUI, A. M.; CARVALHO, M. C. C. G. de; ROCHA, C. S.; DARBEN, L. M.; LOPES-CAITAR, V. S.; AOYAOGI, L. N.; MARCELINO-GUIMARÃES, F. C. Temporal gene expression analysis of effector candidates of Phakopsora pachyrhizi across the infection cycle. In: INTERNATIONAL CONGRESS ON MOLECULAR PLANT-MICROBE INTERACTIONS, 16., 2014, Rhodes. [Abstracts...]. Atenas: International Society of Molecular Plant-Microbe Interactions, 2014.| Biblioteca(s): Embrapa Soja. |
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| 9. | | SILVA, A. G.; HISHINUMA-SILVA, S. M.; LOPES-CAITAR, V. S.; NOMURA, R. G.; DIAS, W. P.; LOPES, I. de O. N.; CARVALHO, M. C. C. G.; MARCELINO-GUIMARÃES, F. C. Análise funcional do gene At4g10250 em resposta à infecção por Meloidogyne javanica em Arabidopsis thaliana. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 13., 2018, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2018. p. 46-52.| Biblioteca(s): Embrapa Soja. |
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| 10. | | DARBEN, L. M.; YOKOYAMA, A.; PARMEZAN, T. R.; LOPES-CAITAR, V. S.; AOYAGI, L. N.; CARVALHO, M. C. C. G. de; GODOY, C. V.; GONELA, A.; SOARES, R. M.; ALMEIDA, A. M. R.; MARCELINO-GUIMARÃES, F. C. Molecular diversity of monouredinial Phakopsora pachyrhizi isolates collected in Brazil. In: WORKSHOP ON BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS, 2013, Ilhéus. The challenge for the 21st century: book of abstracts. [Brasília]: Embrapa: International Advanced Biology Consortium, 2013. p. 56.| Biblioteca(s): Embrapa Soja. |
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| 11. | | YOKOYAMA, A.; DARBEN, L. M.; LOPES-CAITAR, V. S.; AOYAGI, L. N.; CARVALHO, M. C. C. G. de; SOARES, R. M.; ALMEIDA, A. M. R.; MARCELINO-GUIMARÃES, F. C. Diversidade genética de isolados monouredinias de Phakopsora pachyrhizi coletados em diferentes regiões do Brasil. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 8., 2013, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2013. p. 11-15. (Embrapa Soja. Documentos, 339).| Biblioteca(s): Embrapa Soja. |
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| 12. | | AOYAGI, L. N.; LOPES-CAITAR, V. S.; CARVALHO, M. C. C. G. de; DARBEN, L. M.; POLIZEL-PODANOSQUI, A.; KUWAHARA, M. K.; NEPOMUCENO, A. L.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. Genomic and transcriptomic characterization of the transcription factor family R2R3-MYB in soybean and its involvement in the resistance responses to Phakopsora pachyrhizi. Plant Sciense, Limerick, v. 229, p. 32-42, Dec. 2014.| Biblioteca(s): Embrapa Soja. |
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| 13. | | CARVALHO, K.; ABE, V. Y.; DARBEN, L. M.; RINCÃO, M. P.; QI, M.; UTIYAMA, A. S.; CARVALHO, M. C. C. G.; LOPES, I. de O. N.; ABDELNOOR, R. V.; WHITHAM, S. A.; MARCELINO-GUIMARÃES, F. C. Host induced gene silencing in P. pachyrhizi effectors interfere in virulence of soybean attenuating fungal pathogenicity In: IS-MPMI Congress, 18., 2019, Glasgow. [Abstracts...]. [S. l.: s. n.], 2019.| Biblioteca(s): Embrapa Soja. |
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| 14. | | KUMA, K. M.; LOPES-CAITAR, V. S.; ROMERO, C. C. T.; SILVA, S. M. H.; CARVALHO, M. C. C. G.; ABDELNOOR, R. V.; DIAS, W. P.; MARCELINO-GUIMARÃES, F. C. A high efficient protocol for soybean root transformation by Agrobacterium rhizogenes and most stable reference genes for RT-qPCR analysis. Plant Cell Reports, v. 34, n. 11, p. 1987-2000, 2015.| Biblioteca(s): Embrapa Soja. |
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| 15. | | LOPES-CAITAR, V. S.; CARVALHO, M. C. C. G. de; LUANA M. DARBEN; KUWAHARA, M. K.; NEPOMUCENO, A. L.; DIAS, W. P.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. Genome-wide analysis of the Hsp20 gene family in soybean: comprehensive sequence, genomic organization and expression profile analysis under abiotic and biotic stresses. BMC Genomics, v. 14, article 577, 2013. 17 p.| Biblioteca(s): Embrapa Soja. |
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| 16. | | PEREIRA, S. S.; SENA, J. A. D.; RODRIGUES, F. A.; CARVALHO, M. C. C. G.; PEREIRA, R. M.; MARIN, S. R. R.; BINNECK, E.; ABDELNOOR, R. V.; NEPOMUCENO, A. L.; MARCELINO, F. C. Expression profile of soybean MYB family genes under drought conditions. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 221.| Biblioteca(s): Embrapa Soja. |
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| 17. | | DARBEN, L. M.; YOKOYAMA, A.; PARMEZAN, T. R.; KUWAHARA, M. K.; CARVALHO, M. C. C. G. de; GONELA, A.; SOARES, R. M.; ALMEIDA, A. M. R.; GODOY, C. V.; MARCELINO-GUIMARÃES, F. C. Evaluation of virulence of Phakopsora pachyrhizi monourediniais isolates collected in Brazil. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 47.; SIMPÓSIO BRASILEIRO DE MOFO BRANCO, 2014, Londrina. Desafios futuros: anais. Londrina: SBF, 2014. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
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| 18. | | KUMA, K. M.; LOPES-CAITAR, V. S.; ROMERO, C. C. T.; DARBEN, L. M.; SILVA, S. M. H.; CARVALHO, M. C. C. G. de; KUWAHARA, M. K.; OLIVEIRA, M. C. N. de; DIAS, W. P.; MARCELINO-GUIMARÃES, F. C. Functional characterization of soybean GmHsp17.6B and GmHsp22.4 genes in response to Meloidogyne javanica infection. In: WORKSHOP ON BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS, 2013, Ilhéus. The challenge for the 21st century: book of abstracts. [Brasília]: Embrapa: International Advanced Biology Consortium, 2013. p. 34.| Biblioteca(s): Embrapa Soja. |
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| 19. | | CARVALHO, M. C. C. G. de; NASCIMENTO, L. C.; POLIZEL-PODANOSQUI, A. M.; ROCHA, C. S.; DARBEN, L. M.; LOPES-CAITAR, V. S.; CARAZZOLLE, M. F.; OLIVEIRA, M. L. C. S.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C. The transcriptome interactions between Phakopsora pachykopsora pachyrhizi-soybean. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 47.; SIMPÓSIO BRASILEIRO DE MOFO BRANCO, 2014, Londrina. Desafios futuros: anais. Londrina: SBF, 2014. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
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| 20. | | COSTA, R. P. S.; DE CARVALHO, K.; RINCÃO, M. P.; NOMURA, R. B. G.; AVELINO, B. B.; LOPES, I. de O. N.; ABDELNOOR, R. V.; DE CARVALHO, M. C. C. G.; MARCELINO-GUIMARÃES, F. C. Validation of a system for functional gene analysis in P. pachyrhizi by ectopic application of dsRNA. In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 23 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
17/12/2015 |
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Data da última atualização: |
05/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
KUMA, K. M.; LOPES-CAITAR, V. S.; ROMERO, C. C. T.; SILVA, S. M. H.; CARVALHO, M. C. C. G.; ABDELNOOR, R. V.; DIAS, W. P.; MARCELINO-GUIMARÃES, F. C. |
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Afiliação: |
UEL; UEL; CNPSo; UEL; UENP; RICARDO VILELA ABDELNOOR, CNPSO; WALDIR PEREIRA DIAS, CNPSO; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO. |
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Título: |
A high efficient protocol for soybean root transformation by Agrobacterium rhizogenes and most stable reference genes for RT-qPCR analysis. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plant Cell Reports, v. 34, n. 11, p. 1987-2000, 2015. |
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ISSN: |
1432-203X |
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DOI: |
10.1007/s00299-015-1845-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Gene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis in composite plants, mainly under nematode infection. MenosGene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis i... Mostrar Tudo |
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Thesagro: |
Soja. |
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Categoria do assunto: |
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Marc: |
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260 $c2015
520 $aGene functional analyses are essential to the validation of results obtained from in silico and/or geneprospecting studies. Genetic transformation methods that yield tissues of transient expression quickly have been of considerable interest to researchers. Agrobacterium rhizogenes-mediated transformation methods, which are employed to generate plants with transformed roots, have proven useful for the study of stress caused by root phytopathogens via gene overexpression and/or silencing. While some protocols have been adapted to soybean plants, transformation efficiencies remain limited; thus, few viable plants are available for performing bioassays. Furthermore, mRNA analyses that employ reverse transcription quantitative polymerase chain reactions (RT-qPCR) require the use of reference genes with stable expression levels across different organs, development steps and treatments. In the present study, an A. rhizogenes-mediated soybean root transformation approach was optimized. The method delivers significantly higher transformation efficiency levels and rates of transformed plant recovery, thus enhancing studies of soybean abiotic conditions or interactions between phytopathogens, such as nematodes. A 55 % transformation efficiency was obtained following the addition of an acclimation step that involves hydroponics and different selection processes. The present study also validated the reference genes GmELF1-b and GmELF1-a as the most stable to be used in RT-qPCR analysis in composite plants, mainly under nematode infection.
650 $aSoja
700 1 $aLOPES-CAITAR, V. S.
700 1 $aROMERO, C. C. T.
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700 1 $aDIAS, W. P.
700 1 $aMARCELINO-GUIMARÃES, F. C.
773 $tPlant Cell Reports$gv. 34, n. 11, p. 1987-2000, 2015.
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