| |
|
|
| Registros recuperados : 9 | |
| 5. |  | ALMEIDA, M. M. de; CARVALHO, M. A. M. de; MACHADO JÚNIOR, A. A. N.; RIGHI, D. A.; XAVIER, F. G.; CONDE JÚNIOR, A. M.; BOMBONATO, P. P. Efeito do grau de bipartição escrotal sobre a vascularização arterial do escroto de caprinos nativos do Estado do Piauí. Brazilian Journal of Veterinary Research and Animal Science, São Paulo, v. 45, n. 3, p. 167-173, 2008.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
|   |
| 6. | | FERRAZ, M. S.; MORAES JÚNIOR, F. de J.; FEITOSA, M. L. T.; ALMEIDA, H. M. de; BEZERRA, D. de O.; PESSOA, G. T.; ALBUQUERQUE, D. M. de N.; CARVALHO, M. A. M. de. Técnica de fatiamento do ovário para obtenção de oócitos em cutias (Dasyprocta prymnolpha). Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 3, p. 204-208, mar. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
|  |
| 7. | | ALMEIDA, M. M. de; ASSIS NETO, A. C. de; PENNO, A. K.; CONDE JÚNIOR, A. M.; MENEZES, D. J. A. de; PEREIRA, G. R.; AZEVÊDO, L. M. de; CARVALHO, M. A. M. de. Testicular arteries systematization based on different levels of scrotal configuration in caprines. Ciência Rural, Santa Maria, v. 38, n. 5, p. 1308-1312, ago. 2008.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
| |
| 8. | | CARVALHO, Y. K. P. de; ARGÔLO-NETO, N. M.; AMBRÓSIO, C. E.; OLIVEIRA, L. de J. de; ROCHA, A. R. da; SILVA, J. B. da; CARVALHO, M. A. M. de; ALVES, F. R. Isolation, expansion and differentiation of cellular progenitors obbtained from dental pulp of agouti (Dasyprocta prymnolopha Wagler, 1831). Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 6, p. 590-598, jun. 2015| Biblioteca(s): Embrapa Unidades Centrais. |
| |
| 9. | | SOUSA, R. P.; MONTEIRO, H. M. de A.; BEZERRA, D. de O.; SOARES, L. L. da S.; ASSIS NETO, A. C.; RICI, R. E. G.; CONDE JÚNIOR, A. M.; CARVALHO, M. A. M. de. Morphogenesis of the rhea (Rhea americana) respiratory system in different embryonic and foetal stages. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 1, p. 154-166, janeiro 2018. Título em português: Morfogênese do sistema respiratório da ema (Rhea americana) em diferentes estágios embrionários e fetais.| Biblioteca(s): Embrapa Unidades Centrais. |
| |
| Registros recuperados : 9 | |
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Unidades Centrais. |
|
Data corrente: |
30/03/2016 |
|
Data da última atualização: |
30/03/2016 |
|
Autoria: |
CARVALHO, Y. K. P. de; ARGÔLO-NETO, N. M.; AMBRÓSIO, C. E.; OLIVEIRA, L. de J. de; ROCHA, A. R. da; SILVA, J. B. da; CARVALHO, M. A. M. de; ALVES, F. R. |
|
Afiliação: |
YULLA K. P. DE CARVALHO, UFPI; NAPOLEÃO M. ARGÔLO-NETO, UFPI; CARLOS E. AMBRÓSIO, USP; LILIAN DE JESUS DE OLIVEIRA, USP; ANDRESSA R. DA ROCHA, UFPI; JANETE B. DA SILVA, UFPI; MARIA ACELINA M. DE CARVALHO, UFPI; FLÁVIO R. ALVES, UFPI. |
|
Título: |
Isolation, expansion and differentiation of cellular progenitors obbtained from dental pulp of agouti (Dasyprocta prymnolopha Wagler, 1831). |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 6, p. 590-598, jun. 2015 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The study aimed to isolate, expand, differentiate and characterize progenitor cells existent in the dental pulp of agouti. The material was washed with PBS solution and dissociated mechanically with the aid of a scalpel blade on plates containing culture medium D-MEM/F-12, and incubated at 5% CO2-37°C. The growth curve, CFU assay, osteogenic/ adipogenic differentiation and characterization were obtained from the isolation. The cells began to be released from the explant tissue around the 7th day of culture. By day 22 of culture, cells reached 80% confluence. At the UFC test, 81 colonies were counted with 12 days of cultivation. The growth curves before and after freezing showed a regular growth with intense proliferation and clonogenic potential. The cell differentiation showed formation of osteoblasts and fat in culture, starting at 15 days of culture in a specific medium. Flow cytometry (FACs) was as follows: CD34 (positive), CD14 (negative), CD45 (negative), CD73 (positive), CD79 (negative), CD90 (positive), CD105 (positive), demonstrating high specificity and commitment of isolated cells with mesenchymal stem cells strains. These results suggest the existence of a cell population of stem cells with mesenchymal features from the isolated tissue in the explants of agouti dental pulp, a potential model for study of stem cell strains obtained from the pulp tissue. |
|
Palavras-Chave: |
Biologia celular; Cultivo celular; Dasyprocta prymnolopha; Diferenciação celular; MSC da polpa dentária; MSC from dental pulp. |
|
Thesaurus NAL: |
Cell biology; Cell culture; Cell differentiation. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141856/1/Isolation-expansion.pdf
|
|
Marc: |
LEADER 02395naa a2200313 a 4500
001 2042235
005 2016-03-30
008 2015 bl uuuu u00u1 u #d
100 1 $aCARVALHO, Y. K. P. de
245 $aIsolation, expansion and differentiation of cellular progenitors obbtained from dental pulp of agouti (Dasyprocta prymnolopha Wagler, 1831).
260 $c2015
520 $aThe study aimed to isolate, expand, differentiate and characterize progenitor cells existent in the dental pulp of agouti. The material was washed with PBS solution and dissociated mechanically with the aid of a scalpel blade on plates containing culture medium D-MEM/F-12, and incubated at 5% CO2-37°C. The growth curve, CFU assay, osteogenic/ adipogenic differentiation and characterization were obtained from the isolation. The cells began to be released from the explant tissue around the 7th day of culture. By day 22 of culture, cells reached 80% confluence. At the UFC test, 81 colonies were counted with 12 days of cultivation. The growth curves before and after freezing showed a regular growth with intense proliferation and clonogenic potential. The cell differentiation showed formation of osteoblasts and fat in culture, starting at 15 days of culture in a specific medium. Flow cytometry (FACs) was as follows: CD34 (positive), CD14 (negative), CD45 (negative), CD73 (positive), CD79 (negative), CD90 (positive), CD105 (positive), demonstrating high specificity and commitment of isolated cells with mesenchymal stem cells strains. These results suggest the existence of a cell population of stem cells with mesenchymal features from the isolated tissue in the explants of agouti dental pulp, a potential model for study of stem cell strains obtained from the pulp tissue.
650 $aCell biology
650 $aCell culture
650 $aCell differentiation
653 $aBiologia celular
653 $aCultivo celular
653 $aDasyprocta prymnolopha
653 $aDiferenciação celular
653 $aMSC da polpa dentária
653 $aMSC from dental pulp
700 1 $aARGÔLO-NETO, N. M.
700 1 $aAMBRÓSIO, C. E.
700 1 $aOLIVEIRA, L. de J. de
700 1 $aROCHA, A. R. da
700 1 $aSILVA, J. B. da
700 1 $aCARVALHO, M. A. M. de
700 1 $aALVES, F. R.
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 35, n. 6, p. 590-598, jun. 2015
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
