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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
17/05/1993 |
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Data da última atualização: |
17/05/1993 |
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Autoria: |
LOPEZ, M. M.; NAVARRO, L. |
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Afiliação: |
INIA. Centro de Levante (CRIDA 07). Moncada, Valencia, Spain. |
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Título: |
A new in vitro inoculation method for citrus canker diabnosis |
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Ano de publicação: |
0 |
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Fonte/Imprenta: |
Tokyo, Japan: International Society of Citricultural, l981. |
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Volume: |
v.1 |
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Páginas: |
p.399-402 |
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Idioma: |
Inglês |
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Conteúdo: |
Twenty-day-old 'Marsh' grapefruit (Citrus paradisi Macf.) and 'Parson Brown' sweet orange [C. sinensis (L.) osbeck] seedlings growing under sterile conditions in Murashige and Skoog salt solutioons were inoculated by placing a drop of Xanthomonas campestris pv. citri suspension on wounded and unwounded leaves. Inoculated seedlings were incubated at 27 degree C under 16 hr daily exposure to 1,000 lux illumination. Seven to ten days following inoculation, typical symptoms of citrus canker were observed in all inoculated plants. Disease expression appeared sooner and was more severe in wounded than in unwounded plants. Symptoms were not observed in plants inoculated with a strain of Pseudomanas syringae isolated from citrus or with Xamthomonas spp. pathogenic tonon-citrus hosts. Advantages and disadvantages of the method are discussed. |
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Thesaurus Nal: |
quarantine; tissue culture. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01320naa a2200181 a 4500
001 1644002
005 1993-05-17
008 bl uuuu u00u1 u #d
100 1 $aLOPEZ, M. M.
245 $aA new in vitro inoculation method for citrus canker diabnosis
260 $c0
300 $ap.399-402 v.1
490 $vv.1
520 $aTwenty-day-old 'Marsh' grapefruit (Citrus paradisi Macf.) and 'Parson Brown' sweet orange [C. sinensis (L.) osbeck] seedlings growing under sterile conditions in Murashige and Skoog salt solutioons were inoculated by placing a drop of Xanthomonas campestris pv. citri suspension on wounded and unwounded leaves. Inoculated seedlings were incubated at 27 degree C under 16 hr daily exposure to 1,000 lux illumination. Seven to ten days following inoculation, typical symptoms of citrus canker were observed in all inoculated plants. Disease expression appeared sooner and was more severe in wounded than in unwounded plants. Symptoms were not observed in plants inoculated with a strain of Pseudomanas syringae isolated from citrus or with Xamthomonas spp. pathogenic tonon-citrus hosts. Advantages and disadvantages of the method are discussed.
650 $aquarantine
650 $atissue culture
700 1 $aNAVARRO, L.
773 $tTokyo, Japan: International Society of Citricultural, l981.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
