|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
08/12/2023 |
Data da última atualização: |
13/12/2023 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
LACORTE, C. C.; FERREIRA, A. L.; MURAD, A. M.; CUNHA, N. B. da; PINHEIRO, P. V. |
Afiliação: |
CRISTIANO CASTRO LACORTE, Cenargen; AMANDA LOPES FERREIRA, bolsista CNPAF; ALINE MELRO MURAD, UFSC; NICOLAU BRITO DA CUNHA, UNB; PATRICIA VALLE PINHEIRO, CNPAF. |
Título: |
Seed-based production of recombinant proteins. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
In: KOLE, C.; CHAURASIA, A.; HEFFERON, K. L.; PANIGRAHI, J. (ed.). Tools & techniques of plant molecular farming. [S.l.]: Springer Singapore, 2023. Cap. 6. |
Páginas: |
Cap. 6, p. 185-208. |
DOI: |
https://doi.org/10.1007/978-981-99-4859-8_6 |
Idioma: |
Inglês |
Conteúdo: |
The use of plants to produce recombinant proteins has become a promising alternative to current expression systems based on microorganisms and cell cultures. Producing recombinant proteins in plants presents advantages such as reduced upstream costs, lower probability of infection by mammals’ pathogens, and easy scale-up production. Furthermore, plants can make posttranslational modifications and express large and complex proteins. Seeds stand out for their intrinsic characteristics among the plant tissues that can be used for recombinant protein production. In seeds, recombinant proteins are stored in protein storage vacuoles in the endosperm cells. They can be stably maintained for 4–6 years at room temperature without significant loss of protein and biological activity. Progress in methods for genome editing, alteration of the glycosylation pattern of the recombinant proteins produced, and use of protein fusions to aid downstream processes are some trends that shall contribute further to make molecular farming an attractive option for recombinant protein production. This chapter discusses the potential of seeds as a platform for producing recombinant proteins, across the scale-up of the production systems, basic approaches for the purification of recombinant proteins from plant cells, and biosafety issues |
Palavras-Chave: |
Foreign gene expression; Fusion proteins; Heterologous protein production; Plant molecular farming; Recombinant protein purification. |
Thesaurus Nal: |
Biosafety; Glycosylation. |
Categoria do assunto: |
-- X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02273naa a2200277 a 4500 001 2159472 005 2023-12-13 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/978-981-99-4859-8_6$2DOI 100 1 $aLACORTE, C. C. 245 $aSeed-based production of recombinant proteins.$h[electronic resource] 260 $c2023 300 $aCap. 6, p. 185-208. 520 $aThe use of plants to produce recombinant proteins has become a promising alternative to current expression systems based on microorganisms and cell cultures. Producing recombinant proteins in plants presents advantages such as reduced upstream costs, lower probability of infection by mammals’ pathogens, and easy scale-up production. Furthermore, plants can make posttranslational modifications and express large and complex proteins. Seeds stand out for their intrinsic characteristics among the plant tissues that can be used for recombinant protein production. In seeds, recombinant proteins are stored in protein storage vacuoles in the endosperm cells. They can be stably maintained for 4–6 years at room temperature without significant loss of protein and biological activity. Progress in methods for genome editing, alteration of the glycosylation pattern of the recombinant proteins produced, and use of protein fusions to aid downstream processes are some trends that shall contribute further to make molecular farming an attractive option for recombinant protein production. This chapter discusses the potential of seeds as a platform for producing recombinant proteins, across the scale-up of the production systems, basic approaches for the purification of recombinant proteins from plant cells, and biosafety issues 650 $aBiosafety 650 $aGlycosylation 653 $aForeign gene expression 653 $aFusion proteins 653 $aHeterologous protein production 653 $aPlant molecular farming 653 $aRecombinant protein purification 700 1 $aFERREIRA, A. L. 700 1 $aMURAD, A. M. 700 1 $aCUNHA, N. B. da 700 1 $aPINHEIRO, P. V. 773 $tIn: KOLE, C.; CHAURASIA, A.; HEFFERON, K. L.; PANIGRAHI, J. (ed.). Tools & techniques of plant molecular farming. [S.l.]: Springer Singapore, 2023. Cap. 6.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Algodão. |
Data corrente: |
18/03/2008 |
Data da última atualização: |
18/03/2008 |
Tipo da produção científica: |
Documentos |
Autoria: |
CARVALHO, J. M. F. C.; ARAÚJO, S. de S. |
Afiliação: |
Julita Maria Frota Chagas Carvalho, Embrapa Algodão; Silvany de Sousa Araújo, UEPB. |
Título: |
Aplicação do cultivo de embrião zigótico ou imaturo, no melhoramento vegetal. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
Campina Grande: Embrapa Algodão, 2007. |
Páginas: |
35 p. |
Série: |
(Documentos, 170). |
ISSN: |
0103-0205 |
Idioma: |
Português |
Palavras-Chave: |
Condições ambientais de cultivo; Dormência de sementes; Embrião zigótico; Micropropagação clonal; Reguladores de crescimento; Superação de esterelidade. |
Thesagro: |
Cultura de Embrião. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPA/20841/1/DOC170.pdf
|
Marc: |
LEADER 00718nam a2200229 a 4500 001 1277194 005 2008-03-18 008 2007 bl uuuu u0uu1 u #d 022 $a0103-0205 100 1 $aCARVALHO, J. M. F. C. 245 $aAplicação do cultivo de embrião zigótico ou imaturo, no melhoramento vegetal. 260 $aCampina Grande: Embrapa Algodão$c2007 300 $a35 p. 490 $a(Documentos, 170). 650 $aCultura de Embrião 653 $aCondições ambientais de cultivo 653 $aDormência de sementes 653 $aEmbrião zigótico 653 $aMicropropagação clonal 653 $aReguladores de crescimento 653 $aSuperação de esterelidade 700 1 $aARAÚJO, S. de S.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Algodão (CNPA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|