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| Registros recuperados : 151 | |
| Registros recuperados : 151 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
05/01/2004 |
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Data da última atualização: |
27/03/2017 |
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Autoria: |
EGITO, A. A.; ALBUQUERQUE, M. do S. M.; CASTRO, S. T. R.; PAIVA, S. R.; MARQUEZ, J. R. F.; McMANUS, C.; MARIANTE, A. da S.; ABREU, U. P. G.; SANTOS, S. A.; SERENO, J. R.; FIORAVANTI, M. C. S.; VAZ, C. M.; NOBRE, F. V.; OLIVEIRA, J. V.; CARVALHO, J. H. de; COSTA, M. R.; RIBEIRO, M. N.; LARA, M. A. |
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Afiliação: |
Embrapa Recursos Geneticos e Biotecnologia, DF. Embrapa Amazonia Oriental, PA. UnB, DF. Embrapa Pantanal, MS. UFG, GO. Embrapa Pecuaria Sul, RS. UFRGN, RN. Instituo de Zootecnia, SP. Embrapa Meio-Norte, PI. |
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Título: |
Present status of the ANGR DNA Bank in Brazil. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
In: CONGRESO IBEROAMERICANO DE RAZAS CRIOLLAS, 6.; SIMPOSIO IBEROAMERICANO SOBRE CONSERVACION Y UTILIZACION DE RECURSOS ZOOGENETICOS, 4., 2003, Recife. Resumenes de Ponencias y Comunicaciones. Recife: FIRC / CYTED, 2003. nao paginado. |
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Idioma: |
Inglês |
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Conteúdo: |
Ex situ conservation include the cryopreservation of genetic material: semen, oocytes, embryos, somatic cells and DNA (FAO, 1998). Although, it is not possible to regenerate whole animals from isolated DNA, it has been proved that DNA banking was useful in population genetic studies and epidemiological investigations. The Animal Genetics Laboratory (AGL) at Embrapa Genetic Resources and Biotecnology - Cenargen, Brasilia - DF, Brazil, works with genetic characterization of animal populations that belong to the Brazilian conservation program. At the same time, a DNA Bank is being set up, which already has samples from several animals species. The DNA was extracted from cryopreserved leukocyte pellets or butty coats. After extraction it was quantified and checked for its integrity and quality. Each specimen extracted was divided in at least two samples. One of them was used for characterization studies (-20 C) while the other was stored at -20C at the DNA bank. All animals that were collected received a code number and were catalogued in a data bank where all the available information was registered. The refinement of molecular biology techniques increase the usefulness of banked material that will provide a ready reservoir of valuable scientific information. At this time, the AGL DNA bank has specimens of several breeds of six xpecies. Most of these breeds are in risk of extinction and are involved in the Conservation Program of Embrapa/Cenargen. Actually there are 4,036 specimens that are being stored in our DNA Bank. The samples collected represent 9 breeds of sheep (991 specimens), 11 of horses (674 specimens), 4 of native goats (700 specimens), 5 of buffaloes (404 specimens), 3 of donkeys (140 specimens) and 16 of cattle (1127 specimens). The Bank was improved in 110% in the past three years. DNA banking is proving to be useful for characterization of domestic animal population that are in Brazilian conservation program. In near future, it might be the method of choice when many representatives of a breed are to be stored. MenosEx situ conservation include the cryopreservation of genetic material: semen, oocytes, embryos, somatic cells and DNA (FAO, 1998). Although, it is not possible to regenerate whole animals from isolated DNA, it has been proved that DNA banking was useful in population genetic studies and epidemiological investigations. The Animal Genetics Laboratory (AGL) at Embrapa Genetic Resources and Biotecnology - Cenargen, Brasilia - DF, Brazil, works with genetic characterization of animal populations that belong to the Brazilian conservation program. At the same time, a DNA Bank is being set up, which already has samples from several animals species. The DNA was extracted from cryopreserved leukocyte pellets or butty coats. After extraction it was quantified and checked for its integrity and quality. Each specimen extracted was divided in at least two samples. One of them was used for characterization studies (-20 C) while the other was stored at -20C at the DNA bank. All animals that were collected received a code number and were catalogued in a data bank where all the available information was registered. The refinement of molecular biology techniques increase the usefulness of banked material that will provide a ready reservoir of valuable scientific information. At this time, the AGL DNA bank has specimens of several breeds of six xpecies. Most of these breeds are in risk of extinction and are involved in the Conservation Program of Embrapa/Cenargen. Actually there are 4,036 speci... Mostrar Tudo |
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Palavras-Chave: |
ANGR DNA; Genetic. |
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Thesagro: |
Genética; Sêmen. |
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Thesaurus NAL: |
Brazil. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03224naa a2200385 a 4500
001 1803670
005 2017-03-27
008 2003 bl --- 0-- u #d
100 1 $aEGITO, A. A.
245 $aPresent status of the ANGR DNA Bank in Brazil.
260 $c2003
520 $aEx situ conservation include the cryopreservation of genetic material: semen, oocytes, embryos, somatic cells and DNA (FAO, 1998). Although, it is not possible to regenerate whole animals from isolated DNA, it has been proved that DNA banking was useful in population genetic studies and epidemiological investigations. The Animal Genetics Laboratory (AGL) at Embrapa Genetic Resources and Biotecnology - Cenargen, Brasilia - DF, Brazil, works with genetic characterization of animal populations that belong to the Brazilian conservation program. At the same time, a DNA Bank is being set up, which already has samples from several animals species. The DNA was extracted from cryopreserved leukocyte pellets or butty coats. After extraction it was quantified and checked for its integrity and quality. Each specimen extracted was divided in at least two samples. One of them was used for characterization studies (-20 C) while the other was stored at -20C at the DNA bank. All animals that were collected received a code number and were catalogued in a data bank where all the available information was registered. The refinement of molecular biology techniques increase the usefulness of banked material that will provide a ready reservoir of valuable scientific information. At this time, the AGL DNA bank has specimens of several breeds of six xpecies. Most of these breeds are in risk of extinction and are involved in the Conservation Program of Embrapa/Cenargen. Actually there are 4,036 specimens that are being stored in our DNA Bank. The samples collected represent 9 breeds of sheep (991 specimens), 11 of horses (674 specimens), 4 of native goats (700 specimens), 5 of buffaloes (404 specimens), 3 of donkeys (140 specimens) and 16 of cattle (1127 specimens). The Bank was improved in 110% in the past three years. DNA banking is proving to be useful for characterization of domestic animal population that are in Brazilian conservation program. In near future, it might be the method of choice when many representatives of a breed are to be stored.
650 $aBrazil
650 $aGenética
650 $aSêmen
653 $aANGR DNA
653 $aGenetic
700 1 $aALBUQUERQUE, M. do S. M.
700 1 $aCASTRO, S. T. R.
700 1 $aPAIVA, S. R.
700 1 $aMARQUEZ, J. R. F.
700 1 $aMcMANUS, C.
700 1 $aMARIANTE, A. da S.
700 1 $aABREU, U. P. G.
700 1 $aSANTOS, S. A.
700 1 $aSERENO, J. R.
700 1 $aFIORAVANTI, M. C. S.
700 1 $aVAZ, C. M.
700 1 $aNOBRE, F. V.
700 1 $aOLIVEIRA, J. V.
700 1 $aCARVALHO, J. H. de
700 1 $aCOSTA, M. R.
700 1 $aRIBEIRO, M. N.
700 1 $aLARA, M. A.
773 $tIn: CONGRESO IBEROAMERICANO DE RAZAS CRIOLLAS, 6.; SIMPOSIO IBEROAMERICANO SOBRE CONSERVACION Y UTILIZACION DE RECURSOS ZOOGENETICOS, 4., 2003, Recife. Resumenes de Ponencias y Comunicaciones. Recife: FIRC / CYTED, 2003. nao paginado.
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Embrapa Pantanal (CPAP) |
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