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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
05/11/2021 |
Data da última atualização: |
05/11/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
OKINO, C. H.; CAMINOTTO, L. C. F.; KAPRITCHKOFF, R. T. I.; NICIURA, S. C. M.; ESTEVES, S. N.; SANTOS, I. B. DOS; TOSCANO, J. H. B.; OLIVEIRA, M. C. de S.; CHAGAS, A. C. de S. |
Afiliação: |
CINTIA HIROMI OKINO, CPPSE; LARA CRISTINA FRIZONI CAMINOTTO, UNICEP; RAFAELA TAMI IKEDA KAPRITCHKOFF, UNESP; SIMONE CRISTINA MEO NICIURA, CPPSE; SERGIO NOVITA ESTEVES, CPPSE; ISABELLA BARBOSA DOS SANTOS, UNESP; JOAO HENRIQUE BARBOSA TOSCANO, UNESP; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
Título: |
Comparison of ovine B-globin haplotype sequences and a new multiplex PCR for identification. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Veterinary Parasitology, v.300, 109592, dec. 2021. |
Páginas: |
5 p. |
DOI: |
https://doi.org/10.1016/j.vetpar.2021.109592 |
Idioma: |
Inglês |
Conteúdo: |
Deletion of pre-adult BC-globin in sheep harboring BB haplotype of B-globin was associated to decreased tolerance to anemia and hypoxia, and consequently, reduced resistance to Haemonchus contortus infection, which is closely related to severe anemia. Recently, a qPCR using hydrolysis probe was successfully developed for B-globin haplotype identification, and association between resistance against H. contortus and presence of ?A allele was observed in Morada Nova sheep. Thus, this study aimed to better investigate the differences between B-globin haplotypes and to develop a conventional multiplex PCR, as an alternative to qPCR assay for B-globin haplotype identification. A total of 333 Morada Nova lambs had their blood collected and tested by both qPCR and new multiplex PCR, and 100 % of agreement was observed between the results. Since different primers were designed for such assay development, including different target genes, high specificity of both methods may be also highlighted. Three A haplotype samples were submitted to DNA Sanger sequencing of B-globin gene and compared to sequences previously deposited in Genbank. One nucleotide deletion in intronic region was observed only in AA haplotype of Morada Nova animals, while in BB animals the nucleotide remained present. To the best of our knowledge, this is the first report of multiplex conventional PCR for ovine B-globin haplotype identification. The advantages of the developed conventional PCR are reduced reagents costs (less than a half price) and wider reachability, since even labs without real time PCR thermocyclers are able to offer this assay. Therefore, it may become an important tool for sheep producers to improve genetic selection of parasite resistant animals. MenosDeletion of pre-adult BC-globin in sheep harboring BB haplotype of B-globin was associated to decreased tolerance to anemia and hypoxia, and consequently, reduced resistance to Haemonchus contortus infection, which is closely related to severe anemia. Recently, a qPCR using hydrolysis probe was successfully developed for B-globin haplotype identification, and association between resistance against H. contortus and presence of ?A allele was observed in Morada Nova sheep. Thus, this study aimed to better investigate the differences between B-globin haplotypes and to develop a conventional multiplex PCR, as an alternative to qPCR assay for B-globin haplotype identification. A total of 333 Morada Nova lambs had their blood collected and tested by both qPCR and new multiplex PCR, and 100 % of agreement was observed between the results. Since different primers were designed for such assay development, including different target genes, high specificity of both methods may be also highlighted. Three A haplotype samples were submitted to DNA Sanger sequencing of B-globin gene and compared to sequences previously deposited in Genbank. One nucleotide deletion in intronic region was observed only in AA haplotype of Morada Nova animals, while in BB animals the nucleotide remained present. To the best of our knowledge, this is the first report of multiplex conventional PCR for ovine B-globin haplotype identification. The advantages of the developed conventional PCR are reduced reagents co... Mostrar Tudo |
Palavras-Chave: |
Allele; B haplotype; Beta globin C; Morada Nova; Resistance. |
Thesaurus Nal: |
Sheep. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02671naa a2200313 a 4500 001 2135834 005 2021-11-05 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.vetpar.2021.109592$2DOI 100 1 $aOKINO, C. H. 245 $aComparison of ovine B-globin haplotype sequences and a new multiplex PCR for identification.$h[electronic resource] 260 $c2021 300 $a5 p. 520 $aDeletion of pre-adult BC-globin in sheep harboring BB haplotype of B-globin was associated to decreased tolerance to anemia and hypoxia, and consequently, reduced resistance to Haemonchus contortus infection, which is closely related to severe anemia. Recently, a qPCR using hydrolysis probe was successfully developed for B-globin haplotype identification, and association between resistance against H. contortus and presence of ?A allele was observed in Morada Nova sheep. Thus, this study aimed to better investigate the differences between B-globin haplotypes and to develop a conventional multiplex PCR, as an alternative to qPCR assay for B-globin haplotype identification. A total of 333 Morada Nova lambs had their blood collected and tested by both qPCR and new multiplex PCR, and 100 % of agreement was observed between the results. Since different primers were designed for such assay development, including different target genes, high specificity of both methods may be also highlighted. Three A haplotype samples were submitted to DNA Sanger sequencing of B-globin gene and compared to sequences previously deposited in Genbank. One nucleotide deletion in intronic region was observed only in AA haplotype of Morada Nova animals, while in BB animals the nucleotide remained present. To the best of our knowledge, this is the first report of multiplex conventional PCR for ovine B-globin haplotype identification. The advantages of the developed conventional PCR are reduced reagents costs (less than a half price) and wider reachability, since even labs without real time PCR thermocyclers are able to offer this assay. Therefore, it may become an important tool for sheep producers to improve genetic selection of parasite resistant animals. 650 $aSheep 653 $aAllele 653 $aB haplotype 653 $aBeta globin C 653 $aMorada Nova 653 $aResistance 700 1 $aCAMINOTTO, L. C. F. 700 1 $aKAPRITCHKOFF, R. T. I. 700 1 $aNICIURA, S. C. M. 700 1 $aESTEVES, S. N. 700 1 $aSANTOS, I. B. DOS 700 1 $aTOSCANO, J. H. B. 700 1 $aOLIVEIRA, M. C. de S. 700 1 $aCHAGAS, A. C. de S. 773 $tVeterinary Parasitology$gv.300, 109592, dec. 2021.
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Embrapa Pecuária Sudeste (CPPSE) |
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Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
20/02/2013 |
Data da última atualização: |
28/06/2023 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
PEREIRA, L. G. R.; AZEVEDO, J. A. G.; MACHADO, F. S.; CAMPOS, M. M.; GUIMARAES JUNIOR, R.; TOMICH, T. R.; CARVALHO, B. C. de. |
Afiliação: |
LUIZ GUSTAVO RIBEIRO PEREIRA, CNPGL; JOSE AUGUSTO GOMES AZEVEDO; FERNANDA SAMARINI MACHADO, CNPGL; MARIANA MAGALHAES CAMPOS, CNPGL; ROBERTO GUIMARAES JUNIOR, CPAC; THIERRY RIBEIRO TOMICH, CNPGL; BRUNO CAMPOS DE CARVALHO, CNPGL. |
Título: |
Emissão de gases de efeito estufa na pecuaria - uma análise ambiental e conceitual. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
In: FERNANDES, E. N.; MARTINS, C. E.; NOBRE, M. M.; NOVAES, E.; LOPES, F. C. F.; MENDONCA, L. C.; PEREIRA, L. G. R.; MACHADO, M. A.; TEIXEIRA, S. R.; TALONE, A.; SILVA, F. H.; FIORINI, J. P.; MARTINS, M. C. (ed.). Desafios para a sustentabilidade ambiental, social e econômica da cadeia produtiva do leite. Juiz de Fora: Embrapa Gado de Leite, 2012. |
Páginas: |
p. 101-130. |
Idioma: |
Português |
Palavras-Chave: |
Demanda para alimentos; Proteina animal. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/950229/1/Emissao-de-gases-de-efeito-estufa-na-pecuaria.pdf
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Marc: |
LEADER 00965naa a2200217 a 4500 001 1950229 005 2023-06-28 008 2012 bl uuuu u00u1 u #d 100 1 $aPEREIRA, L. G. R. 245 $aEmissão de gases de efeito estufa na pecuaria - uma análise ambiental e conceitual.$h[electronic resource] 260 $c2012 300 $ap. 101-130. 653 $aDemanda para alimentos 653 $aProteina animal 700 1 $aAZEVEDO, J. A. G. 700 1 $aMACHADO, F. S. 700 1 $aCAMPOS, M. M. 700 1 $aGUIMARAES JUNIOR, R. 700 1 $aTOMICH, T. R. 700 1 $aCARVALHO, B. C. de 773 $tIn: FERNANDES, E. N.; MARTINS, C. E.; NOBRE, M. M.; NOVAES, E.; LOPES, F. C. F.; MENDONCA, L. C.; PEREIRA, L. G. R.; MACHADO, M. A.; TEIXEIRA, S. R.; TALONE, A.; SILVA, F. H.; FIORINI, J. P.; MARTINS, M. C. (ed.). Desafios para a sustentabilidade ambiental, social e econômica da cadeia produtiva do leite. Juiz de Fora: Embrapa Gado de Leite, 2012.
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