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| 15. |  | GRUCHOUSKEI, L.; CARVALHO, A. L. de; VIOTT, A. de M.; DELGADO, L. E. da S.; KRASINSKI, F.; MATOS, M. R. de; CALEFFO, T. Cistomatose de glândula apócrina em onça-pintada (Panthera onca). Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 9, p. 991-994, setembro. 2017. Título em inglês: Apocrine cystomatosis in a jaguar (Panthera onca).| Biblioteca(s): Embrapa Unidades Centrais. |
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| 17. | | DIAS, M. J.; TANEZINI, C. A.; D'ALESSANDRO, W. T.; OLIVEIRA, A. B. C. de; ROCHA, J. de M.; PONTES, I. dos S.; CARVALHO, A. L. de; SOUSA, J. T. de. Mineral characteristics of raw goat milk from the region of Goiania, Brazil. Australian Journal of Animal Science, v. 7, n. 1, p. 63-68, 1994.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 18. | | MIRANDA, M. A. de L.; ESCOBAR, A. F.; CARVALHO, A. L. de; CORREA, A. M.; FRAGA, C. C.; CURY, F. M.; PIRES, J. A.; PAZZINI, J. J.; MAKASHIMA, N.; PASSOS, W. A. Padronizacao de produtos horticolas. Sao Paulo: Secretaria da Agricultura, 1974. 54p.| Biblioteca(s): Embrapa Hortaliças. |
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| 19. | | CARVALHO, A. L. de A.; REZENDE, L. C. de; COSTA, L. B.; HALFELD-VIEIRA, B. de A.; PINTO, Z. V.; MORANDI, M. A. B.; MEDEIROS, F. H. V.; BETTIOL, W. Optimizing the mass production of Clonostachys rosea by liquid-state fermentation. Biological Control, v. 118, p. 16-25, 2018.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 20. | | REZENDE, L. C. de; CARVALHO, A. L. de A.; COSTA, L. B.; HALFELD-VIEIRA, B. de A.; SILVA, L. G.; PINTO. Z. V.; MORANDI, M. A. B.; MEDEIROS, F. H. V. de; MASCARIN, G. M.; BETTIOL, W. Optimizing mass production of Trichoderma asperelloides by submerged liquid fermentation and its antagonism against Sclerotinia sclerotiorum. World Journal of Microbiology & Biotechnology, v. 36, n. 8, article 113, 2020.| Biblioteca(s): Embrapa Meio Ambiente. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
15/10/2020 |
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Data da última atualização: |
15/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
REZENDE, L. C. de; CARVALHO, A. L. de A.; COSTA, L. B.; HALFELD-VIEIRA, B. de A.; SILVA, L. G.; PINTO. Z. V.; MORANDI, M. A. B.; MEDEIROS, F. H. V. de; MASCARIN, G. M.; BETTIOL, W. |
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Afiliação: |
LARISSA CASTRO DE REZANDE, UFLA; ANDRÉ LUIZ DE ANDRADE CARVALHO, UFLA; LÚCIO BERTOLDO COSTA, FCA-UNESP; BERNARDO DE ALMEIDA HALFELD VIEIRA, CNPMA; LUCAS GUEDES SILVA, FCA-UNESP; ZAYAME VEGETTE PINTO, FCA-UNESP; MARCELO AUGUSTO BOECHAT MORANDI, CNPMA; FLÁVIO HENRIQUE VASCONCELOS DE MEDEIROS, UFLA; GABRIEL MOURA MASCARIN, CNPMA; WAGNER BETTIOL, CNPMA. |
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Título: |
Optimizing mass production of Trichoderma asperelloides by submerged liquid fermentation and its antagonism against Sclerotinia sclerotiorum. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
World Journal of Microbiology & Biotechnology, v. 36, n. 8, article 113, 2020. |
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ISSN: |
1573-0972 |
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DOI: |
https://doi.org/10.1007/s11274-020-02882-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Commercial products based on Trichoderma are obtained mainly from solid-state fermentation. Submerged liquid fermentation is the most appropriate method compared to the solid medium for large-scale production of Trichoderma spp. The present study aimed to optimize the combination of key variables that influence the liquid fermentation process of Trichoderma asperelloides LQC-96 for conidial production coupled with its efficiency in the control of Sclerotinia sclerotiorum. In addition, we verified whether the optimized culture conditions can be used for the conidial production of Trichoderma erinaceum T-12 and T-18 and Trichoderma harzianum T-15. Fermentation studies were performed in shake flasks following a planned experimental design to reduce the number of tests and consumable costs. The effect of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on conidial production were assessed, which of pH was the only meaningful factor contributing to increased conidial production of T. asperelloides LQC-96. From the five variables studied initially, pH and C:N ratio were further used in the second design (rotational central composite design-RCCD). Hence, the best conditions for the production of T. asperelloides LQC-96 conidia by liquid fermentation consisted of initial pH of 3.5, C:N ratio of 200:1 at 30 °C, without glycerol, and under 24 h photoperiod. The highest conidial concentration was observed after seven days of fermentation. Under these optimal conditions, T. erinaceum T-12 and T-18, and T. harzianum T-15 were also cultivated, but only LQC-96 efficiently parasitized S. sclerotiorum, precluding sclerotium myceliogenic germination. Our findings propose optimal fermentation conditions that maximize conidial production of T. asperelloides as a potential biofungicide against S. sclerotiorum. MenosAbstract: Commercial products based on Trichoderma are obtained mainly from solid-state fermentation. Submerged liquid fermentation is the most appropriate method compared to the solid medium for large-scale production of Trichoderma spp. The present study aimed to optimize the combination of key variables that influence the liquid fermentation process of Trichoderma asperelloides LQC-96 for conidial production coupled with its efficiency in the control of Sclerotinia sclerotiorum. In addition, we verified whether the optimized culture conditions can be used for the conidial production of Trichoderma erinaceum T-12 and T-18 and Trichoderma harzianum T-15. Fermentation studies were performed in shake flasks following a planned experimental design to reduce the number of tests and consumable costs. The effect of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on conidial production were assessed, which of pH was the only meaningful factor contributing to increased conidial production of T. asperelloides LQC-96. From the five variables studied initially, pH and C:N ratio were further used in the second design (rotational central composite design-RCCD). Hence, the best conditions for the production of T. asperelloides LQC-96 conidia by liquid fermentation consisted of initial pH of 3.5, C:N ratio of 200:1 at 30 °C, without glycerol, and under 24 h photoperiod. The highest conidial concentration was observed after seven days of fermentation. Under these opt... Mostrar Tudo |
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Palavras-Chave: |
Shite mold; Submerged spores. |
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Thesagro: |
Fermentação; Fungo Para Controle Biológico; Trichoderma. |
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Thesaurus NAL: |
Biological control; Biological control agents; Bioreactors; Experimental design; Mass rearing; Molds (fungi); Solid state fermentation. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
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024 7 $ahttps://doi.org/10.1007/s11274-020-02882-7$2DOI
100 1 $aREZENDE, L. C. de
245 $aOptimizing mass production of Trichoderma asperelloides by submerged liquid fermentation and its antagonism against Sclerotinia sclerotiorum.$h[electronic resource]
260 $c2020
520 $aAbstract: Commercial products based on Trichoderma are obtained mainly from solid-state fermentation. Submerged liquid fermentation is the most appropriate method compared to the solid medium for large-scale production of Trichoderma spp. The present study aimed to optimize the combination of key variables that influence the liquid fermentation process of Trichoderma asperelloides LQC-96 for conidial production coupled with its efficiency in the control of Sclerotinia sclerotiorum. In addition, we verified whether the optimized culture conditions can be used for the conidial production of Trichoderma erinaceum T-12 and T-18 and Trichoderma harzianum T-15. Fermentation studies were performed in shake flasks following a planned experimental design to reduce the number of tests and consumable costs. The effect of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on conidial production were assessed, which of pH was the only meaningful factor contributing to increased conidial production of T. asperelloides LQC-96. From the five variables studied initially, pH and C:N ratio were further used in the second design (rotational central composite design-RCCD). Hence, the best conditions for the production of T. asperelloides LQC-96 conidia by liquid fermentation consisted of initial pH of 3.5, C:N ratio of 200:1 at 30 °C, without glycerol, and under 24 h photoperiod. The highest conidial concentration was observed after seven days of fermentation. Under these optimal conditions, T. erinaceum T-12 and T-18, and T. harzianum T-15 were also cultivated, but only LQC-96 efficiently parasitized S. sclerotiorum, precluding sclerotium myceliogenic germination. Our findings propose optimal fermentation conditions that maximize conidial production of T. asperelloides as a potential biofungicide against S. sclerotiorum.
650 $aBiological control
650 $aBiological control agents
650 $aBioreactors
650 $aExperimental design
650 $aMass rearing
650 $aMolds (fungi)
650 $aSolid state fermentation
650 $aFermentação
650 $aFungo Para Controle Biológico
650 $aTrichoderma
653 $aShite mold
653 $aSubmerged spores
700 1 $aCARVALHO, A. L. de A.
700 1 $aCOSTA, L. B.
700 1 $aHALFELD-VIEIRA, B. de A.
700 1 $aSILVA, L. G.
700 1 $aPINTO. Z. V.
700 1 $aMORANDI, M. A. B.
700 1 $aMEDEIROS, F. H. V. de
700 1 $aMASCARIN, G. M.
700 1 $aBETTIOL, W.
773 $tWorld Journal of Microbiology & Biotechnology$gv. 36, n. 8, article 113, 2020.
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