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Registro Completo |
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Biblioteca(s): |
Embrapa Acre. |
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Data corrente: |
06/11/2019 |
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Data da última atualização: |
02/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, J. C. de; RUFINO, P. B.; AZÊVEDO, H. S. F. da S.; SOUSA, A. C. B. de; ASSIS, G. M. L. de; SILVA, L. M. da; SEBBENN, A. M.; CAMPOS, T. de. |
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Afiliação: |
Jônatas Chagas de Oliveira, Universidade Federal do Acre (Ufac); Polinar Bandeira Rufino, Faculdade Meta; Hellen Sandra Freires da Silva Azêvedo, Fundação Oswaldo Cruz; Adna Cristina Barbosa de Sousa, Universidade Federal da Paraíba (UFPB); GISELLE MARIANO LESSA DE ASSIS, CPAF-AC; LUCIELIO MANOEL DA SILVA, CPAF-AC; Alexandre Magno Sebbenn, Instituto Florestal de São Paulo; TATIANA DE CAMPOS, CPAF-AC. |
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Título: |
Inferring mating system parameters in forage peanut, Arachis pintoi, for Brazilian Amazon conditions. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Acta Amazonica, v. 49, n. 4, p. 277-282, 2019. |
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ISSN: |
0044-5967 (print) / 1809-4392 (online) |
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DOI: |
10.1590/1809-4392201900200 |
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Idioma: |
Inglês |
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Conteúdo: |
The search for alternatives to increase productivity and sustainability of livestock production in the Amazon region without increasing deforestation is challenging. Mixed pastures of grasses with forage peanut (Arachis pintoi) have shown positive economic impacts. However, gaps in the knowledge of the reproductive biology of A. pintoi have limited the development of new cultivars adapted to the environmental variations in the Brazilian Amazon. Pasture consortiums of Brachiaria humidicola with forage peanuts (cv. Mandobi) resulted in a 42% increase in weight gain productivity. New cultivars better adapted to the Amazon climate should bring even greater gains. We evaluated the mating system in twenty A. pintoi accessions, and approximately 40 offspring per accession genotyped with eight microsatellites (or markers). The parameters of genetic diversity and inbreeding, the outcrossing rate and coancestry were calculated. The observed heterozygosity was significantly higher and the fixation index was significantly lower in adults compared with the offspring. The crossing rate was variable among genotypes (2 to 80%), and the mean outcrossing rate was 36%. These results indicate that pollinator presence in pastures can influence gene flow in A. pintoi more than expected. Arachis pintoi presented a mixed mating system with a predominance of selfing, and families presented inbreeding and different levels of relatedness. New strategies of genotype conservation are needed to avoid pollinator-mediated crossing between accessions. A busca por alternativas para aumentar a produtividade e a sustentabilidade da pecuária na região Amazônica sem aumentar o desmatamento é desafiadora. Pastagens consorciadas de gramíneas com amendoim forrageiro (Arachis pintoi) têm mostrado impactos econômicos positivos. Porém, lacunas no conhecimento da biologia reprodutiva do amendoim forrageiro tem limitado o desenvolvimento de novas cultivares adaptadas as variações ambientais na Amazônia brasileira. Pastos consorciados de Brachiaria humidicola com amendoim forrageiro (cv. Mandobi) apresentaram cerca de 42% de aumento na produtividade do ganho de peso. Novas cultivares mais adaptadas ao clima amazônico, poderão trazer ganhos ainda maiores. O objetivo desse estudo foi avaliar o sistema reprodutivo em vinte acessos de A. pintoi e aproximadamente 40 progênies por acesso, genotipadas com oito microssatélites (ou marcadores). Os parâmetros de diversidade genética e endogamia, taxa de cruzamento e coancestria foram calculados. A heterozigosidade observada foi significativamente maior e o índice de fixação foi significativamente menor nos adultos comparado às progênies. A taxa de cruzamento foi variável entre os genótipos (2 a 80%) e a média da taxa de cruzamento foi 36%. Esses resultados indicam que a presença de polinizadores em pastagens pode influenciar o fluxo gênico em A. pintoi mais do que o esperado. Arachis pintoi apresentou um sistema de cruzamento misto com predominância de autofecundação e as famílias apresentaram endogamia e diferentes níveis de parentesco. Novas estratégias de conservação de genótipos são necessárias para evitar polinizadores mediadores de cruzamento entre os acessos. MenosThe search for alternatives to increase productivity and sustainability of livestock production in the Amazon region without increasing deforestation is challenging. Mixed pastures of grasses with forage peanut (Arachis pintoi) have shown positive economic impacts. However, gaps in the knowledge of the reproductive biology of A. pintoi have limited the development of new cultivars adapted to the environmental variations in the Brazilian Amazon. Pasture consortiums of Brachiaria humidicola with forage peanuts (cv. Mandobi) resulted in a 42% increase in weight gain productivity. New cultivars better adapted to the Amazon climate should bring even greater gains. We evaluated the mating system in twenty A. pintoi accessions, and approximately 40 offspring per accession genotyped with eight microsatellites (or markers). The parameters of genetic diversity and inbreeding, the outcrossing rate and coancestry were calculated. The observed heterozygosity was significantly higher and the fixation index was significantly lower in adults compared with the offspring. The crossing rate was variable among genotypes (2 to 80%), and the mean outcrossing rate was 36%. These results indicate that pollinator presence in pastures can influence gene flow in A. pintoi more than expected. Arachis pintoi presented a mixed mating system with a predominance of selfing, and families presented inbreeding and different levels of relatedness. New strategies of genotype conservation are needed to avoid pol... Mostrar Tudo |
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Palavras-Chave: |
Aclimatación; Amendoim forrageiro; Arachis pintoi cv Mandobi; Cacahuetes forrajeros; Coancestria; Cross rate; Fitomejoramiento; Forage peanut; Heterocigosidad; Heterozigozidade; Leguminosas forrajeras; Marcador microssatélite; Pastoreo mixto; Pastos forrajeros; Rendimiento de los cultivos; Repeticiones de microsatélite; Tasa de cruce; Taxa de cruzamento; Variación genética. |
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Thesagro: |
Aclimatação; Brachiaria Humidicola; Capim Quicuio; Cruzamento Vegetal; Endogamia; Gramínea Forrageira; Leguminosa Forrageira; Marcador Genético; Melhoramento Genético Vegetal; Pastagem Consorciada; Produtividade; Taxa; Variação Genética. |
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Thesaurus Nal: |
Acclimation; Crop yield; Forage grasses; Forage legumes; Genetic variation; Heterozygosity; Inbreeding; Microsatellite repeats; Mixed grazing; Plant breeding; Urochloa humidicola. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/204322/1/26897.pdf
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Marc: |
LEADER 05445naa a2200745 a 4500
001 2113957
005 2021-07-02
008 2019 bl uuuu u00u1 u #d
022 $a0044-5967 (print) / 1809-4392 (online)
024 7 $a10.1590/1809-4392201900200$2DOI
100 1 $aOLIVEIRA, J. C. de
245 $aInferring mating system parameters in forage peanut, Arachis pintoi, for Brazilian Amazon conditions.$h[electronic resource]
260 $c2019
520 $aThe search for alternatives to increase productivity and sustainability of livestock production in the Amazon region without increasing deforestation is challenging. Mixed pastures of grasses with forage peanut (Arachis pintoi) have shown positive economic impacts. However, gaps in the knowledge of the reproductive biology of A. pintoi have limited the development of new cultivars adapted to the environmental variations in the Brazilian Amazon. Pasture consortiums of Brachiaria humidicola with forage peanuts (cv. Mandobi) resulted in a 42% increase in weight gain productivity. New cultivars better adapted to the Amazon climate should bring even greater gains. We evaluated the mating system in twenty A. pintoi accessions, and approximately 40 offspring per accession genotyped with eight microsatellites (or markers). The parameters of genetic diversity and inbreeding, the outcrossing rate and coancestry were calculated. The observed heterozygosity was significantly higher and the fixation index was significantly lower in adults compared with the offspring. The crossing rate was variable among genotypes (2 to 80%), and the mean outcrossing rate was 36%. These results indicate that pollinator presence in pastures can influence gene flow in A. pintoi more than expected. Arachis pintoi presented a mixed mating system with a predominance of selfing, and families presented inbreeding and different levels of relatedness. New strategies of genotype conservation are needed to avoid pollinator-mediated crossing between accessions. A busca por alternativas para aumentar a produtividade e a sustentabilidade da pecuária na região Amazônica sem aumentar o desmatamento é desafiadora. Pastagens consorciadas de gramíneas com amendoim forrageiro (Arachis pintoi) têm mostrado impactos econômicos positivos. Porém, lacunas no conhecimento da biologia reprodutiva do amendoim forrageiro tem limitado o desenvolvimento de novas cultivares adaptadas as variações ambientais na Amazônia brasileira. Pastos consorciados de Brachiaria humidicola com amendoim forrageiro (cv. Mandobi) apresentaram cerca de 42% de aumento na produtividade do ganho de peso. Novas cultivares mais adaptadas ao clima amazônico, poderão trazer ganhos ainda maiores. O objetivo desse estudo foi avaliar o sistema reprodutivo em vinte acessos de A. pintoi e aproximadamente 40 progênies por acesso, genotipadas com oito microssatélites (ou marcadores). Os parâmetros de diversidade genética e endogamia, taxa de cruzamento e coancestria foram calculados. A heterozigosidade observada foi significativamente maior e o índice de fixação foi significativamente menor nos adultos comparado às progênies. A taxa de cruzamento foi variável entre os genótipos (2 a 80%) e a média da taxa de cruzamento foi 36%. Esses resultados indicam que a presença de polinizadores em pastagens pode influenciar o fluxo gênico em A. pintoi mais do que o esperado. Arachis pintoi apresentou um sistema de cruzamento misto com predominância de autofecundação e as famílias apresentaram endogamia e diferentes níveis de parentesco. Novas estratégias de conservação de genótipos são necessárias para evitar polinizadores mediadores de cruzamento entre os acessos.
650 $aAcclimation
650 $aCrop yield
650 $aForage grasses
650 $aForage legumes
650 $aGenetic variation
650 $aHeterozygosity
650 $aInbreeding
650 $aMicrosatellite repeats
650 $aMixed grazing
650 $aPlant breeding
650 $aUrochloa humidicola
650 $aAclimatação
650 $aBrachiaria Humidicola
650 $aCapim Quicuio
650 $aCruzamento Vegetal
650 $aEndogamia
650 $aGramínea Forrageira
650 $aLeguminosa Forrageira
650 $aMarcador Genético
650 $aMelhoramento Genético Vegetal
650 $aPastagem Consorciada
650 $aProdutividade
650 $aTaxa
650 $aVariação Genética
653 $aAclimatación
653 $aAmendoim forrageiro
653 $aArachis pintoi cv Mandobi
653 $aCacahuetes forrajeros
653 $aCoancestria
653 $aCross rate
653 $aFitomejoramiento
653 $aForage peanut
653 $aHeterocigosidad
653 $aHeterozigozidade
653 $aLeguminosas forrajeras
653 $aMarcador microssatélite
653 $aPastoreo mixto
653 $aPastos forrajeros
653 $aRendimiento de los cultivos
653 $aRepeticiones de microsatélite
653 $aTasa de cruce
653 $aTaxa de cruzamento
653 $aVariación genética
700 1 $aRUFINO, P. B.
700 1 $aAZÊVEDO, H. S. F. da S.
700 1 $aSOUSA, A. C. B. de
700 1 $aASSIS, G. M. L. de
700 1 $aSILVA, L. M. da
700 1 $aSEBBENN, A. M.
700 1 $aCAMPOS, T. de
773 $tActa Amazonica$gv. 49, n. 4, p. 277-282, 2019.
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Registro original: |
Embrapa Acre (CPAF-AC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
17/11/2021 |
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Data da última atualização: |
17/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SANTOS, J. D. R.; BATISTA, R. I. T. P.; UNGERFELD, R.; TAIRA, A. R.; ESPÍRITO SANTO, C. G. do; SOUZA-FABJAN, J. M. G.; FERNANDES, D. A. de M.; BALARO, M. F. A.; COSENTINO, I. O.; BRAIR, V. L.; PINTO, P. H. N.; CARVALHO, A. B. da S.; FONSECA, J. F. da; BRANDÃO, F. Z. |
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Afiliação: |
JULIANA DANTAS RODRIGUES SANTOS, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; RODOLFO UNGERFELD, Universidad de la República - Montevideo, Uruguay; AUGUSTO RYONOSUKE TAIRA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; CAROLINE GOMES DO ESPÍRITO SANTO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; JOANNA MARIA GONÇALVES SOUZA-FABJAN, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; DANIEL ANDREWS DE MOURA FERNANDES, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; MÁRIO FELIPE ALVAREZ BALARO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; ISABEL OLIVEIRA COSENTINO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; VIVIANE LOPES BRAIR, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; PEDRO HENRIQUE NICOLAU PINTO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; ANA BEATRIZ DA SILVA CARVALHO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; JEFERSON FERREIRA DA FONSECA, CNPC; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil. |
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Título: |
Hormonal protocol used for cervical dilation in ewes does not affect morphological embryo quality but reduces recovery rate and temporarily alters gene expression. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Veterinary Record, e1064, 2021. |
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DOI: |
https://doi.org/10.1002/vetr.1064 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Background: Information on the impact of hormonal protocols for cervical dilation on the quality of ovine embryos is scarce. Methods: To compare the quality of embryos after cervical dilation protocol, ewes (n = 64) were allocated into either a treated group (100 ?g estradiol benzoate intravenous and 0.12 mg cloprostenol intramuscularly, 12 hours before embryo collection plus 100 iu oxytocin intravenous 15 minutes before the collection procedure) or a control group (saline). Luteal function was analysed using ultrasonography and P4 measurement. Some collected embryos were frozen/thawed for gene expression, others were cultured in vitro, frozen/thawed for gene expression, and the remaining embryos were fixed for the apoptosis test (TUNEL test). Results: The treatment reduced fluid (p=0.04) and structure (p=0.03) recovery rates, but the morphological quality, development stage, and apoptosis incidence of the embryos were not affected by treatment. The corpora lutea of the control group had greater blood perfusion (p = 0.002) and greater P4 concentrations at 6, 9, and 12 h after the treatment (p < 0.0001). The expression of BAX, BCL2, PRDX1, and HSP90 genes were not affected by the treatment. However, the embryos in the treated group had fewer NANOG and OCT4 transcripts than control embryos (p = 0.008; p = 0.006, respectively). After culture, there was no difference between the groups in any gene. Conclusion: The hormonal protocol for cervical dilation reduced the efficiency of embryo collection. In addition, the treatment induced luteolysis and a transient alteration of embryo gene expression, however there were no detectable changes in embryo morphological quality, development stage, or incidence of apoptosis. MenosAbstract: Background: Information on the impact of hormonal protocols for cervical dilation on the quality of ovine embryos is scarce. Methods: To compare the quality of embryos after cervical dilation protocol, ewes (n = 64) were allocated into either a treated group (100 ?g estradiol benzoate intravenous and 0.12 mg cloprostenol intramuscularly, 12 hours before embryo collection plus 100 iu oxytocin intravenous 15 minutes before the collection procedure) or a control group (saline). Luteal function was analysed using ultrasonography and P4 measurement. Some collected embryos were frozen/thawed for gene expression, others were cultured in vitro, frozen/thawed for gene expression, and the remaining embryos were fixed for the apoptosis test (TUNEL test). Results: The treatment reduced fluid (p=0.04) and structure (p=0.03) recovery rates, but the morphological quality, development stage, and apoptosis incidence of the embryos were not affected by treatment. The corpora lutea of the control group had greater blood perfusion (p = 0.002) and greater P4 concentrations at 6, 9, and 12 h after the treatment (p < 0.0001). The expression of BAX, BCL2, PRDX1, and HSP90 genes were not affected by the treatment. However, the embryos in the treated group had fewer NANOG and OCT4 transcripts than control embryos (p = 0.008; p = 0.006, respectively). After culture, there was no difference between the groups in any gene. Conclusion: The hormonal protocol for cervical dilation reduced the eff... Mostrar Tudo |
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Thesaurus NAL: |
Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02718naa a2200301 a 4500
001 2136205
005 2021-11-17
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1002/vetr.1064$2DOI
100 1 $aSANTOS, J. D. R.
245 $aHormonal protocol used for cervical dilation in ewes does not affect morphological embryo quality but reduces recovery rate and temporarily alters gene expression.$h[electronic resource]
260 $c2021
520 $aAbstract: Background: Information on the impact of hormonal protocols for cervical dilation on the quality of ovine embryos is scarce. Methods: To compare the quality of embryos after cervical dilation protocol, ewes (n = 64) were allocated into either a treated group (100 ?g estradiol benzoate intravenous and 0.12 mg cloprostenol intramuscularly, 12 hours before embryo collection plus 100 iu oxytocin intravenous 15 minutes before the collection procedure) or a control group (saline). Luteal function was analysed using ultrasonography and P4 measurement. Some collected embryos were frozen/thawed for gene expression, others were cultured in vitro, frozen/thawed for gene expression, and the remaining embryos were fixed for the apoptosis test (TUNEL test). Results: The treatment reduced fluid (p=0.04) and structure (p=0.03) recovery rates, but the morphological quality, development stage, and apoptosis incidence of the embryos were not affected by treatment. The corpora lutea of the control group had greater blood perfusion (p = 0.002) and greater P4 concentrations at 6, 9, and 12 h after the treatment (p < 0.0001). The expression of BAX, BCL2, PRDX1, and HSP90 genes were not affected by the treatment. However, the embryos in the treated group had fewer NANOG and OCT4 transcripts than control embryos (p = 0.008; p = 0.006, respectively). After culture, there was no difference between the groups in any gene. Conclusion: The hormonal protocol for cervical dilation reduced the efficiency of embryo collection. In addition, the treatment induced luteolysis and a transient alteration of embryo gene expression, however there were no detectable changes in embryo morphological quality, development stage, or incidence of apoptosis.
650 $aSheep
700 1 $aBATISTA, R. I. T. P.
700 1 $aUNGERFELD, R.
700 1 $aTAIRA, A. R.
700 1 $aESPÍRITO SANTO, C. G. do
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aFERNANDES, D. A. de M.
700 1 $aBALARO, M. F. A.
700 1 $aCOSENTINO, I. O.
700 1 $aBRAIR, V. L.
700 1 $aPINTO, P. H. N.
700 1 $aCARVALHO, A. B. da S.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDÃO, F. Z.
773 $tVeterinary Record, e1064, 2021.
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