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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
23/01/2017 |
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Data da última atualização: |
23/01/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CALVI, G. P.; AUD, F. F.; FERRAZ, I. D. K.; PRITCHARD, H. W.; KRANNER, I. |
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Afiliação: |
G. P. CALVI, INPA; FABIANA FERRAZ AUD, CNPMF; I. D. K. FERRAZ, INPA; H. W. PRITCHARD, Royal Botanic Gardens; I. KRANNER, Royal Botanic Gardens. |
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Título: |
Analyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Plant Biology, n. 19, p. 6-13, German Botanical Society; d The Royal Botanical Society of the Netherlands, 2017. |
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ISSN: |
1435-8603 |
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Idioma: |
Inglês |
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Conteúdo: |
The use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (c-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (EGSSG/2GSH) was less negative than 215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in EGSSG/2GSH, and seed death. In contrast, the cyst(e)ine half-cell reduction potential (ECySS/2CYS) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at EGSSG/2GSH values between 180 and 160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towards more negative values. We discuss the contribution of cellular pH to EGSSG/2GSH and recommend E. stipitata as a recalcitrant seed model to study stress response on a single seed basis. MenosThe use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (c-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (EGSSG/2GSH) was less negative than 215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in EGSSG/2GSH, and seed death. In contrast, the cyst(e)ine half-cell reduction potential (ECySS/2CYS) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at EGSSG/2GSH values between 180 and 160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towar... Mostrar Tudo |
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Thesagro: |
Semente. |
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Categoria do assunto: |
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Marc: |
LEADER 02347naa a2200193 a 4500
001 2061470
005 2017-01-23
008 2017 bl uuuu u00u1 u #d
022 $a1435-8603
100 1 $aCALVI, G. P.
245 $aAnalyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination.$h[electronic resource]
260 $c2017
520 $aThe use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (c-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (EGSSG/2GSH) was less negative than 215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in EGSSG/2GSH, and seed death. In contrast, the cyst(e)ine half-cell reduction potential (ECySS/2CYS) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at EGSSG/2GSH values between 180 and 160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towards more negative values. We discuss the contribution of cellular pH to EGSSG/2GSH and recommend E. stipitata as a recalcitrant seed model to study stress response on a single seed basis.
650 $aSemente
700 1 $aAUD, F. F.
700 1 $aFERRAZ, I. D. K.
700 1 $aPRITCHARD, H. W.
700 1 $aKRANNER, I.
773 $tPlant Biology$gn. 19, p. 6-13, German Botanical Society; d The Royal Botanical Society of the Netherlands, 2017.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 505 | |
| 501. |  | MOREIRA, C. T.; FARIAS NETO, A. L. de; SOUZA, P. I. de M. de; SILVA, S. A. da; MONTEIRO, P. M. F. O.; NEIVA, L. C. S.; NUNES, M. R.; SILVA, L. O.; PIMENTA, C. B.; TOLEDO, R. M. C. P.; VIEIRA, N. E.; NUNES JÚNIOR, J.; SEII, A. H.; ARANTES, N. E.; BROGIN, R. L.; MELLO FILHO, O. L.; CARNEIRO, G. E. de S.; DIAS, W. P.; MEYER, M. C.; ANDRADE, P. J. M.; SOARES, R. M.; KASTER, M.; ALMEIDA, A. M. R.; ARIAS, C. A. A.; TOLEDO, J. F. F.; PÍPOLO, A. E.; ABDELNOOR, R. V.; CARRÃO-PANIZZI, M. C.; MOREIRA, J. U. V.; OLIVEIRA, M. F.; TEIXEIRA, R. N. Cultivar de soja BRSGO 8660: indicação para os estados de Minas Gerais, Mato Grosso, Goiás e para o Distrito Federal. In: CONGRESSO BRASILEIRO DE SOJA, 5.; MERCOSOJA 2009, 2009, Goiânia. Resumos... Londrina: Embrapa Soja, 2009. 258 p. p. 232. Resumo 424.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| 502. | | MELLO FILHO, O. L.; TOLEDO, R. M. C. P.; BORGES, A. O.; VIEIRA, N. E.; NUNES JÚNIOR, J.; CARNEIRO, G. E. S.; NUNES, M. R.; NEIVA, L. C. S.; MONTEIRO, P. M. F. O.; COUTO, M. B.; DIAS, W. P.; SOARES, R. M.; ALMEIDA, A. M. R.; MOREIRA, J. U. V.; OLIVEIRA, M. F.; BROGIN, R. L.; PIPOLO, A. E.; ARIAS, C. A. A.; KASTER, M.; ABDELNOOR, R. V.; CARRÃO-PANIZZI, M. C.; CÂMARA, A. R.; SEII, A. H.; MEYER, M. C.; VAZ BISNETA, M. Cultivar de soja BRSGO 8661 RR: indicação para os estados de Goiás e do Tocantins. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 31., 2010, Brasília, DF. Resumos... Londrina: Embrapa Soja, 2010. p. 324-325. Editores técnicos: Adilson de Oliveira Junior, Odilon Ferreira Saraiva, Regina Maria Villas Bôas de Campo Leite, César de Castro, Jussara Flores de Oliveira Arbues, Wellington Cavalcanti.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 503. | | MONTEIRO, P. M. F. O.; VIEIRA, N. E.; MELLO FILHO, O. L. de; NUNES JÚNIOR, J.; TOLEDO, R. M. C. P.; BORGES, A. O.; CARNEIRO, G. E. S.; NUNES M. R.; NEIVA, L. C. S.; COUTO, M. B.; DIAS, W. P.; SOARES, R. M.; ALMEIDA, A. M. R.; MOREIRA, J. U. V.; OLIVEIRA, M. F.; BROGIN, R. L.; PIPOLO, A. E.; ARIAS, C. A. A.; KASTER, M.; ABDELNOOR, R. V.; CARRÃO-PANIZZI, M. C.; CÂMARA, A. R.; SEII, A. H.; MEYER, M. C.; VAZ BISNETA, M. Cultivar de soja BRSGO 8860RR: indicação para os estados de Goiás e do Tocantins. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 31., 2010, Brasília, DF. Resumos... Londrina: Embrapa Soja, 2010. p. 253-254. Editores técnicos: Adilson de Oliveira Junior, Odilon Ferreira Saraiva, Regina Maria Villas Bôas de Campo Leite, César de Castro, Jussara Flores de Oliveira Arbues, Wellington Cavalcanti.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 504. | | MOREIRA. C. T.; FARIAS NETO, A. L.; SOUZA, P. I. M.; ABUD, S.; SILVA, N. S.; MONTEIRO, P. M. F. O.; NEIVA, L. C. S.; ALMEIDA, A. M. R.; DIAS, W. P.; SOARES, R. M.; NUNES, M. R.; VIEIRA, N. E.; SILVA, L. O.; TOLEDO, R. M. C. P.; NUNES JÚNIOR, J.; ARANTES, N. E.; BROGIN, R.; MELLO FILHO, O. L.; ARIAS, C. A. A.; TOLEDO, J. F. F.; CARNEIRO, G. E. de S.; KASTER, M.; PÍPOLO, A. E.; MOREIRA, J. U. V.; OLIVEIRA, M. F.; CARRÃO-PANIZZI, M. C.; ABDELNOOR, R. V.; ALMEIDA, L. A. Cultivar de soja BRS Juliana RR: extensão de indicação para os estados da Bahia, do Tocantins e do Maranhão. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 30., 2008, Rio Verde. Resumos... Londrina: Embrapa Soja, 2008. p. 240-241. (Embrapa Soja. Documentos, 304).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados; Embrapa Soja. |
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| 505. | | SOUZA, P. I. de M. de; TOLEDO, J. F. F. de; MOREIRA, C. T.; FARIAS NETO, A.; SILVA, S. A. da; ARANTES, N. E.; MELLO FILHO, O. L. de; CARNEIRO, G. E. de S.; MONTEIRO, P. M. F. O.; ARIAS, C. A. A.; KASTER, M.; OLIVEIRA, M. F. de; PIPOLO, A. E.; BROGIN, R. L.; NUNES JÚNIOR, J.; ABDELNOOR, R. V.; ALMEIDA, A. M. R.; MEYER, M. C.; ANDRADE, P. J. M.; CARRAO-PANIZZI, M. C.; DIAS, W. P.; MOREIRA, J. U. V.; NEIVA, L. C. da S.; NUNES, M. R.; SEII, A. H.; SILVA, L. O. e; SILVA, N. S.; TEIXEIRA, R. N.; SOARES, R. M.; TOLEDO, R. M. C. P.; VIEIRA, N. E. Variedade de soja resistente à ferrugem (BRSGRO 7560): posicionamento da nova tecnologia. In: REUNIÃO DO CONSÓRCIO ANTIFERRUGEM SAFRA 2008-09, 2009, Londrina. Resumos... Londrina: Embrapa Soja, 2009. p. 89-91. (Embrapa Soja. Documentos, 315). Editado por Claudia Vieira Godoy, Claudine Dinali Santos Seixas, Rafael Moreira Soares.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 505 | |
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